Graft can induce inheritable variations in the progenies of the scion plants. Seedling of mungbean ( Vigna radiata (L.) Wilczek) was grafted onto the stem of sweet potato ( Ipomoea batatas (L.) Lam.). The growth...Graft can induce inheritable variations in the progenies of the scion plants. Seedling of mungbean ( Vigna radiata (L.) Wilczek) was grafted onto the stem of sweet potato ( Ipomoea batatas (L.) Lam.). The growth of the scion was maintained until the scion produced selfed seeds. We sowed the seeds for several generations under normal conditions. Distinct genetic variations appeared in the progenies. Similar variations did not appear in the generations of the scion sowed normally without graft. The variations seemed to be induced by the graft and they inherited steadily. For understanding the possible mechanism of the phenomenon (graft_induced inheritable variation), we analyzed the cytoplasmic and genomic DNA of the variations. The results showed that there was no restriction fragment length polymorphism (RFLP) in the cytoplasmic DNA between the original scion and the variation. However, significant difference between the scion and variation was recognized by random amplified polymorphic DNA (RAPD) analysis. In addition, there was no evidence that indicated the gene transformation from stock to scion. Our results suggest that the non_specific grafting has a pragmatic potential for plant breeding and crop improvement and, the genetic variation seems not to be caused simply by DNA transformation but most likely the stress induced mutation.展开更多
This study aimed to remediate in-situ leach mining contaminated soil by amendment-plant synergism. The results showed that plant species exhibited ex-tremely significant effects on the concentration of nitrate nitroge...This study aimed to remediate in-situ leach mining contaminated soil by amendment-plant synergism. The results showed that plant species exhibited ex-tremely significant effects on the concentration of nitrate nitrogen; to be specific, the concentration of nitrate nitrogen in soil planted with wheat was reduced from 692.19 mg/kg to lower than 100 mg/kg; when the mass ratio of amendment to soil reached 3:50 and the amendment particle size was 1-2 mm, the concentration of nitrate ni-trogen in soil planted with wheat was reduced to 43 mg/kg. The amendment type exhibited extremely significant effects on the concentration of ammonium nitrogen; to be specific, when the mass ratio of amendment to soil reached 10:50, the concen-tration of ammonium nitrogen in soil added with 2-3 mm zeolite was reduced from 23 593.75 to 3 300 mg/kg on day 15. Amendments and plants mainly exhibited desorption performance for sulfate radical in soil, and the amendment type extreme-ly significantly affected the concentration of sulfate radical; to be specific, the con-centration of sulfate radical in soil added with limestone increased from 370 mg/kg to 900 mg/kg on day 7.展开更多
This study was conducted to study the feasibility of biochar in soil im- provement and tobacco quality. The black soil in NinganCountry of Mudanjiang was used as the material in this study, and the effects of biochar ...This study was conducted to study the feasibility of biochar in soil im- provement and tobacco quality. The black soil in NinganCountry of Mudanjiang was used as the material in this study, and the effects of biochar on tobacco-planting soil C/N, soil microorganisms, the development of tobacco, and chemical compo- nents and neutral aroma components in flue-cured tobacco werestudied. The results showed that the application of biochar at a rate of 1 800 kg/hm2 could increase soil C/N by 31.79%, and the quantity of actinomycetes in the soil was 3.8 times as much as that in control. The growth wasobviously better after biochar application, plant height and effective leaf number were significantly higher than those ofcontrol, but total sugar and nicotine were not significantly different. The application of biochar at a rate of 1 200 kg/hm2 increased the potassium content by 11%, the application of biochar at a rate of 2 400 kg/hm2 improved total sugar content by 5.40%, and the application of biochar at a rate of 1 800 kg/hm2 made the tobacco solanone content 1.97 times as that of control. Comprehensive studies showed that the application of biochar 1 200-1 800 kg/hm2 could improvesoilenvironment, and promote tobacco growth and quality.展开更多
[Objective] The study aimed to introduce a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [ Method ] The genomic DNAs were extracted from tender leaves of 24 peanut cuhiv...[Objective] The study aimed to introduce a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [ Method ] The genomic DNAs were extracted from tender leaves of 24 peanut cuhivars and from the liver, lung and kidney of white mouse through the specifically modified CTAB method. The DNAs were run on agarose gel, next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. [ Result] The clear and orderly bands were observed in gel detection, and the values of OD200/OD200 for DNAs extracted via modified CTAB method were between 1.77 - 1.83. The DNAs performed well in PCR amplification. [ Conclusion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification.展开更多
The effects of five amendments such as acetic acid(AA), citric acid (CA), ethylenediamine tetraacetic acid (EDTA), sepiolite and phosphogypsum on growth and metal uptake of giant reed (Arundo donax L.) grown o...The effects of five amendments such as acetic acid(AA), citric acid (CA), ethylenediamine tetraacetic acid (EDTA), sepiolite and phosphogypsum on growth and metal uptake of giant reed (Arundo donax L.) grown on soil contaminated by arsenic (As), cadmium (Cd) and lead (Pb) were studied. The results showed that the shoot biomass of giant reed was enhanced by 24.8% and 15.0%, while superoxide mutase and catalase activities slightly varied when adding 5.0 mmol/kg CA and 2.5 mol/kg EDTA to soil as compared to the control, respectively. The concentrations of As, Cd and Pb in shoots were remarkably increased by the addition of 2.5 mmol/kg AA and CA, 5.0 mmol/kg EDTA, and 4.0 g/kg sepiolite as compared to the control. The accumulations of As and Cd were also significantly enhanced in the above condition, while the shoot Pb accumulation was noticeably enhanced by amending with 4.0 g/kg sepiolite and 8.0 g/kg phosphogysum, respectively. The results suggested that AA, CA and sepiolite could be used as optimum soil amendments for giant reed remediation system.展开更多
Objective: To study the telomerase activity in human renal cell carcinoma and to evaluate the correlation with the clinicobiologic features of the neogrowth.Methods: The telomerase activity was studied by means of a m...Objective: To study the telomerase activity in human renal cell carcinoma and to evaluate the correlation with the clinicobiologic features of the neogrowth.Methods: The telomerase activity was studied by means of a modified telomeric repeat amplification protocol (TRAP) in 32 renal cell carcinoma tissues, 32 normal renal tissues and 32 paracancer tissues and its correlation with the clinicopathologic features of the tumor was evaluated.Results: Telomerase activity was strongly positive in 17, positive in 12 and negative in 3 cases of renal cell carcinoma tissues, the total positive rate being 91%. Telomerase activity was weakly positive (6%) in only 2 out of 32 samples of normal renal cortex tissues and positive in 6 paracancer tissues (19%), the difference was conspicuous (P<0.01).Conclusion: The positive rate of telomerase activity was significantly higher in renal cell carcinoma tissues and might serve as a prognostic marker for estimating the biologic characteristics of renal cell carcinoma.展开更多
This paper summarized the history and present condition of studying and utilizing entomopathogenic nematodes at home and abroad, expounded its taxonomy, life cycle and the mechanism with symbiotic bacteria killing hos...This paper summarized the history and present condition of studying and utilizing entomopathogenic nematodes at home and abroad, expounded its taxonomy, life cycle and the mechanism with symbiotic bacteria killing host insect. Taxonomy, mycelial form, pathogenic function and anti-bacteria function of symbiotic bacteria were introduced. Production and utilization of entomopathogenic nematodes, the characteristic genetic improvement by use of biological engineering technology, as well as the existing problem and applying foreground were also discussed.展开更多
Biochar(BC)is widely applied in agricultural production for its multiple uses such as carbon sequestration.However,application of BC alone has limited effect on soil fertility and crop yield,especially alkaline soil.T...Biochar(BC)is widely applied in agricultural production for its multiple uses such as carbon sequestration.However,application of BC alone has limited effect on soil fertility and crop yield,especially alkaline soil.Therefore,a pot experiment on Chinese cabbage(Brassica rapa var.glabra)was carried out in this study to investigate the effect of BC applied with organic fertilizer(OF)on alkaline soil properties and crop yield.To be specific,BC and OF were respectively applied at 0,1%,2%,and 3%,and Chinese cabbage was transplanted and cultivated for 2.5 months.Results showed that BC and OF increased the content of both organic matter and available P in alkaline soil(P<0.05).Moreover,the application of OF alone decreased the pH value but raised available N content of alkaline soil,and the application of only BC demonstrated the contrary effect(P<0.05).OF significantly improved crop yield(P<0.05),but the effect of BC was insignificant.Crop yield was the highest under the treatment of 1%BC and 3%OF.Thus,BC had limited effect on alkaline soil fertility and crop yield,but the application with OF was a good option for ameliorating alkaline soil and raising crop yield.展开更多
Bauxite residue is an alkaline waste material in the process of alumina production due to its characteristics of higher salinity and alkalinity,which results in environmental issues and extremely restricts the sustain...Bauxite residue is an alkaline waste material in the process of alumina production due to its characteristics of higher salinity and alkalinity,which results in environmental issues and extremely restricts the sustainable development of alumina industries.In this work,we conduct a column experiment to study the effects of two amendments on aggregate stability and variations in alkaline minerals of bauxite residue.The two amendments are phosphogypsum(PG)and phosphogypsum and vermicompost(PVC).The dominant fraction in aggregate is 1–0.25 mm in diameter on the surface,which takes up 39.34%,39.38%,and 44.51%for CK,PG,and PVC,respectively.Additions of PG and PVC decreased pH,EC,ESP,exchangeable Na^+concentration and the percentage of alkaline minerals,and then increased exchangeable Ca^2+concentration in bauxite residue.There was significant positive correlation between pH and exchangeable Na^+concentration,the percentage of cancrinite,tricalcium aluminate and calcite;while negative correlation was found in pH value versus exchangeable Ca^2+concentration.Theses findings confirmed that additions of phosphogypsum and vermicompost have a stimulative effect on aggregate stability in bauxite residue.In particular,amendment neutralization(phosphogypsum+vermicompost)in column represents an advantage for large-scale simulation of vegetation rehabilitate in bauxite residue disposal areas.展开更多
文摘Graft can induce inheritable variations in the progenies of the scion plants. Seedling of mungbean ( Vigna radiata (L.) Wilczek) was grafted onto the stem of sweet potato ( Ipomoea batatas (L.) Lam.). The growth of the scion was maintained until the scion produced selfed seeds. We sowed the seeds for several generations under normal conditions. Distinct genetic variations appeared in the progenies. Similar variations did not appear in the generations of the scion sowed normally without graft. The variations seemed to be induced by the graft and they inherited steadily. For understanding the possible mechanism of the phenomenon (graft_induced inheritable variation), we analyzed the cytoplasmic and genomic DNA of the variations. The results showed that there was no restriction fragment length polymorphism (RFLP) in the cytoplasmic DNA between the original scion and the variation. However, significant difference between the scion and variation was recognized by random amplified polymorphic DNA (RAPD) analysis. In addition, there was no evidence that indicated the gene transformation from stock to scion. Our results suggest that the non_specific grafting has a pragmatic potential for plant breeding and crop improvement and, the genetic variation seems not to be caused simply by DNA transformation but most likely the stress induced mutation.
基金Supported by National Natural Science Foundation of China(Grant No.21067003,5136-4015)Natural Science Foundation of Jiangxi Province(Grant No.20114BAB203024)National High-Tech Research and Development Program of China(Grant No.2012BAC11B07)~~
文摘This study aimed to remediate in-situ leach mining contaminated soil by amendment-plant synergism. The results showed that plant species exhibited ex-tremely significant effects on the concentration of nitrate nitrogen; to be specific, the concentration of nitrate nitrogen in soil planted with wheat was reduced from 692.19 mg/kg to lower than 100 mg/kg; when the mass ratio of amendment to soil reached 3:50 and the amendment particle size was 1-2 mm, the concentration of nitrate ni-trogen in soil planted with wheat was reduced to 43 mg/kg. The amendment type exhibited extremely significant effects on the concentration of ammonium nitrogen; to be specific, when the mass ratio of amendment to soil reached 10:50, the concen-tration of ammonium nitrogen in soil added with 2-3 mm zeolite was reduced from 23 593.75 to 3 300 mg/kg on day 15. Amendments and plants mainly exhibited desorption performance for sulfate radical in soil, and the amendment type extreme-ly significantly affected the concentration of sulfate radical; to be specific, the con-centration of sulfate radical in soil added with limestone increased from 370 mg/kg to 900 mg/kg on day 7.
基金Supported by Scientific and Technological Development Project of Tobacco Industry in Helongjiang Province(HYK[2015]59)~~
文摘This study was conducted to study the feasibility of biochar in soil im- provement and tobacco quality. The black soil in NinganCountry of Mudanjiang was used as the material in this study, and the effects of biochar on tobacco-planting soil C/N, soil microorganisms, the development of tobacco, and chemical compo- nents and neutral aroma components in flue-cured tobacco werestudied. The results showed that the application of biochar at a rate of 1 800 kg/hm2 could increase soil C/N by 31.79%, and the quantity of actinomycetes in the soil was 3.8 times as much as that in control. The growth wasobviously better after biochar application, plant height and effective leaf number were significantly higher than those ofcontrol, but total sugar and nicotine were not significantly different. The application of biochar at a rate of 1 200 kg/hm2 increased the potassium content by 11%, the application of biochar at a rate of 2 400 kg/hm2 improved total sugar content by 5.40%, and the application of biochar at a rate of 1 800 kg/hm2 made the tobacco solanone content 1.97 times as that of control. Comprehensive studies showed that the application of biochar 1 200-1 800 kg/hm2 could improvesoilenvironment, and promote tobacco growth and quality.
文摘[Objective] The study aimed to introduce a rapid and effective method that is suitable for extracting genomic DNA from animal and plant. [ Method ] The genomic DNAs were extracted from tender leaves of 24 peanut cuhivars and from the liver, lung and kidney of white mouse through the specifically modified CTAB method. The DNAs were run on agarose gel, next detected by DNA/Protein analyzer. Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method. [ Result] The clear and orderly bands were observed in gel detection, and the values of OD200/OD200 for DNAs extracted via modified CTAB method were between 1.77 - 1.83. The DNAs performed well in PCR amplification. [ Conclusion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification.
基金Project (2012BAC09B04) supported by National Key Technology Research and Development Program of the Ministry of Science and Technology of ChinaProject (2010-277-027) supported by Science and Technology Foundation of Environmental Protection in Hunan Province,ChinaProject (2011SK3262) supported by Science and Technology Planning of Hunan Province,China
文摘The effects of five amendments such as acetic acid(AA), citric acid (CA), ethylenediamine tetraacetic acid (EDTA), sepiolite and phosphogypsum on growth and metal uptake of giant reed (Arundo donax L.) grown on soil contaminated by arsenic (As), cadmium (Cd) and lead (Pb) were studied. The results showed that the shoot biomass of giant reed was enhanced by 24.8% and 15.0%, while superoxide mutase and catalase activities slightly varied when adding 5.0 mmol/kg CA and 2.5 mol/kg EDTA to soil as compared to the control, respectively. The concentrations of As, Cd and Pb in shoots were remarkably increased by the addition of 2.5 mmol/kg AA and CA, 5.0 mmol/kg EDTA, and 4.0 g/kg sepiolite as compared to the control. The accumulations of As and Cd were also significantly enhanced in the above condition, while the shoot Pb accumulation was noticeably enhanced by amending with 4.0 g/kg sepiolite and 8.0 g/kg phosphogysum, respectively. The results suggested that AA, CA and sepiolite could be used as optimum soil amendments for giant reed remediation system.
文摘Objective: To study the telomerase activity in human renal cell carcinoma and to evaluate the correlation with the clinicobiologic features of the neogrowth.Methods: The telomerase activity was studied by means of a modified telomeric repeat amplification protocol (TRAP) in 32 renal cell carcinoma tissues, 32 normal renal tissues and 32 paracancer tissues and its correlation with the clinicopathologic features of the tumor was evaluated.Results: Telomerase activity was strongly positive in 17, positive in 12 and negative in 3 cases of renal cell carcinoma tissues, the total positive rate being 91%. Telomerase activity was weakly positive (6%) in only 2 out of 32 samples of normal renal cortex tissues and positive in 6 paracancer tissues (19%), the difference was conspicuous (P<0.01).Conclusion: The positive rate of telomerase activity was significantly higher in renal cell carcinoma tissues and might serve as a prognostic marker for estimating the biologic characteristics of renal cell carcinoma.
基金This paper was supported by Natural Science Founda-tion of Heilongjiang Province (No.C-9735).
文摘This paper summarized the history and present condition of studying and utilizing entomopathogenic nematodes at home and abroad, expounded its taxonomy, life cycle and the mechanism with symbiotic bacteria killing host insect. Taxonomy, mycelial form, pathogenic function and anti-bacteria function of symbiotic bacteria were introduced. Production and utilization of entomopathogenic nematodes, the characteristic genetic improvement by use of biological engineering technology, as well as the existing problem and applying foreground were also discussed.
文摘Biochar(BC)is widely applied in agricultural production for its multiple uses such as carbon sequestration.However,application of BC alone has limited effect on soil fertility and crop yield,especially alkaline soil.Therefore,a pot experiment on Chinese cabbage(Brassica rapa var.glabra)was carried out in this study to investigate the effect of BC applied with organic fertilizer(OF)on alkaline soil properties and crop yield.To be specific,BC and OF were respectively applied at 0,1%,2%,and 3%,and Chinese cabbage was transplanted and cultivated for 2.5 months.Results showed that BC and OF increased the content of both organic matter and available P in alkaline soil(P<0.05).Moreover,the application of OF alone decreased the pH value but raised available N content of alkaline soil,and the application of only BC demonstrated the contrary effect(P<0.05).OF significantly improved crop yield(P<0.05),but the effect of BC was insignificant.Crop yield was the highest under the treatment of 1%BC and 3%OF.Thus,BC had limited effect on alkaline soil fertility and crop yield,but the application with OF was a good option for ameliorating alkaline soil and raising crop yield.
基金Projects(41701587,41877511)supported by the National Natural Science Foundation of China
文摘Bauxite residue is an alkaline waste material in the process of alumina production due to its characteristics of higher salinity and alkalinity,which results in environmental issues and extremely restricts the sustainable development of alumina industries.In this work,we conduct a column experiment to study the effects of two amendments on aggregate stability and variations in alkaline minerals of bauxite residue.The two amendments are phosphogypsum(PG)and phosphogypsum and vermicompost(PVC).The dominant fraction in aggregate is 1–0.25 mm in diameter on the surface,which takes up 39.34%,39.38%,and 44.51%for CK,PG,and PVC,respectively.Additions of PG and PVC decreased pH,EC,ESP,exchangeable Na^+concentration and the percentage of alkaline minerals,and then increased exchangeable Ca^2+concentration in bauxite residue.There was significant positive correlation between pH and exchangeable Na^+concentration,the percentage of cancrinite,tricalcium aluminate and calcite;while negative correlation was found in pH value versus exchangeable Ca^2+concentration.Theses findings confirmed that additions of phosphogypsum and vermicompost have a stimulative effect on aggregate stability in bauxite residue.In particular,amendment neutralization(phosphogypsum+vermicompost)in column represents an advantage for large-scale simulation of vegetation rehabilitate in bauxite residue disposal areas.