聚焦“设、评、改”的生物学实验教学实践

本文在分析生物学实验教学中存在的问题的基础上,阐述了设、评、改贯通的实验教学的内涵及过程。通过课堂教学实践,可有效实现聚焦高阶思维,促进学生的深度学习,提升核心素养的效果。

《城乡建设》创刊65周年大事记(1956-2021)

1956年,《城乡建设》杂志的前身《城市建设》杂志创刊。1955年4月6日,国务院第三办公室印发《关于成立城市建设总局的报告》。1956年5月,撤消城市建设总局,改设城市建设部。万里同志任城市建设部部长。1958年,城市建设部与建筑工程部、建筑材料工业部合并为建筑工程部。

同一控制下的企业吸收合并在实践中的应用

本文通过一个实际案例,详细描述了在同一控制条件下的法人企业改设为分公司的过程,同时引入了吸收式合并的概念,使得国企改制的举措及实际操作流程跃然纸上,具有一定的参考价值。

Reconstruction of HP Intein with Overlap-extension PCR

[Objective] This study aimed to reconstruct the HP intein by overlap-exten- sion PCR. [Method] Several primers were designed according to the principle of overlap-extension PCR that the repeat sequences were overlapped and served as the template to the other DNA strand in amplification. Then, several rounds of over- lap PCR reaction were conducted to mutate the four cysteines in the HP intein into serines, and introduce a linker sequence containing a tag for product detection and purification. [Result] DNA sequencing analysis proved that the four cysteines in the HP intein were successfully mutated into serines; the PCR precursor fragments were also rejoined into an intact HP gene fragment, without introducing any other unex- pected mutation; the DNA linker of 46 bp was successfully inserted into the de- signed HP gene sequences, without any mutation and base pair mismatch. [Conclu- sion] The HP intein was rapidly reconstructed by overlap-extension PCR in this study, which not only expands the application of overlap PCR in protein engineering, but also provides a simple, efficient and highly accurate tool for the reconstruction and modification of intein.

China's Infrastructure Development and Supply-Side Structural Reforms

Despite significant improvements in transport, energy and information communication technology （ICT） infrastructures, inadequacies still exist in China＇s infrastructure development in light of emerging demand and industrial transition and upgrade. China lags far behind advanced economies such as the United States, Germany and Japan in terms of the quality of transport infrastructures except railroad,＂ electricity infrastructure is insufficient and service efficiency needs improvement; ICT infrastructure requires all-round upgrade and gaps in Internet bandwidth are striking. This paper suggests that, based on the needs of supply-side structural reform, China must reform its investment and financing systems to enhance the efficiency of infrastructure investment. Efforts must be made to enhance air transport and port infrastructure to support industrial upgrade; to develop the energy Internet to address emerging demands; and to forge a new generation of ICT infrastructure with industrial Internet as the priority to prepare China for the new round of industrial competition.

试论明清地方行政建置——以江西定南地区为例

明清时期,新设厅县的建置,使得边远偏僻之所形成了相对独立和完整的行政区域,促进了国家政权力量的深入,维持了统治的稳定。本文旨在探究江西定南地区从设县到改厅的沿革过程,复原出初设县时的辖境、官署人员配备,以及县(厅)之下的基层地域组织等情况,从而有利于进一步加深对明清时期江西地方行政体制的认识。

Study on key techniques on ecological renovation design of existing buildings in China

With the rapid development of China economy and critical ecological situation in China,ecological renovation of existing buildings has become a special important topic. Key techniques on ecological renovation design of existing buildings in China are explored in this paper. Special attention is paid to specific treatments suitable for China.

Security Analysis and Enhanced Design of a Dynamic Block Cipher

There are a lot of security issues in block cipher algorithm.Security analysis and enhanced design of a dynamic block cipher was proposed.Firstly,the safety of ciphertext was enhanced based on confusion substitution of S-box,thus disordering the internal structure of data blocks by four steps of matrix transformation.Then,the diffusivity of ciphertext was obtained by cyclic displacement of bytes using column ambiguity function.The dynamic key was finally generated by using LFSR,which improved the stochastic characters of secret key in each of round of iteration.The safety performance of proposed algorithm was analyzed by simulation test.The results showed the proposed algorithm has a little effect on the speed of encryption and decryption while enhancing the security.Meanwhile,the proposed algorithm has highly scalability,the dimension of S-box and the number of register can be dynamically extended according to the security requirement.

Development of XY scanner with minimized coupling motions for high-speed atomic force microscope

The design and fabrication processes of a novel scanner with minimized coupling motions for a high-speed atomic force microscope （AFM） were addressed. An appropriate design modification was proposed through the analyses of the dynamic characteristics of existing linear motion stages using a dynamic analysis program, Recurdyn. Because the scanning speed of each direction may differ, the linear motion stage for a high-speed scanner was designed to have different resonance frequencies for the modes, with one dominant displacement in the desired directions. This objective was achieved by using one-direction flexure mechanisms for each direction and mounting one stage for fast motion on the other stage for slow motion. This unsymmetrical configuration separated the frequencies of two vibration modes with one dominant displacement in each desired direction, and hence suppressed the coupling between motions in two directions. A pair of actuators was used for each axis to decrease the crosstalk between the two motions and give a sufficient force to actuate the slow motion stage, which carried the fast motion stage, A lossy material, such as grease, was inserted into the flexure hinge to suppress vibration problems that occurred when using an input triangular waveforrn. With these design modifications and the vibration suppression method, a novel scanner with a scanning speed greater than 20 Hz is achieved.

Investigation of a Design Modification for Double Helical Gears Reducing Vibration and Noise

To reduce vibration and noise and increase transmission efficiency, a three segment method for modifying the pinion profile was proposed. Cutter surface equations were deduced by changing the shape of the cutter-edge, substituting three segment parabolas for the line. The influence of longitudinal tooth modifications on tooth surface load distributions was discussed. Transmission error minimization and uniformity of tooth surface load distribution were chosen as optimization goals and the modified parameters were obtained by applying the complex method. Finally, an experiment comparing the loaded transmission error, vibration, and noise both before and after modifications was carried out. The results indicate that the modified design is reliable.

Application of polypropylene biofilm carrier modified by biocompatible, hydrophlic and magnetic additives to aerobic process

The designed new-type polypropylene biomass carrier/packing was modified by biocompatible, hydrophilic and magnetic additives and further charged with a permanent magnet （〈5.0×10^-3 Tesla）. The cartier was applied to biological contact-oxidation of synthesized wastewater. The results showed that the surface contact angle of modified carrier was reduced 22%, and the adsorbed water content increased by 300%-500%. The period of biofilm formation reduced from 7-9 days to 3-4 days after modification and magnetization. The hiodegradation process was reduced from 22.5 h to 10.5 h for completely removal of 500 mg/L CODcr by using the modified carrier. When operating in continuous mode, the modified carrier can reach a maximum CODa removal rate of 99% at the air-water ration of 40：1, while the original polypropylene packing may only achieve the maximum degradation efficiency of 79% at the gas-water ratio of 30：1.

State-of-the-art imaging techniques in endoscopic ultrasound

Endoscopic ultrasound (EUS) has recently evolved through technological improvement of equipment,with a major clinical impact in digestive and mediastinal diseases.State-of-the-art EUS equipment now includes real-time sono-elastography,which might be useful for a better characterization of lesions and increased accuracy of differential diagnosis (for e.g.lymph nodes or focal pancreatic lesions).Contrast-enhanced EUS imaging is also available,and is already being used for the differential diagnosis of focal pancreatic masses.The recent development of low mechanical index contrast harmonic EUS imaging offers hope for improved diagnosis,staging and monitoring of anti-angiogenic treatment.Tridimensional EUS (3D-EUS) techniques can be applied to enhance the spatial understanding of EUS anatomy,especially for improved staging of tumors,obtained through a better assessment of the relationship with major surrounding vessels.Despite the progress gained through all these imaging techniques,they cannot replace cytological or histological diagnosis.However,real-time optical histological diagnosis can be achieved through the use of single-fiber confocal laser endomicroscopy techniques placed under real-time EUS-guidance through a 22G needle.Last,but not least,EUS-assisted natural orifice transluminal endoscopic surgery (NOTES) procedures offer a whole new area of imaging applications,used either for combination of NOTES peritoneoscopy and intraperitoneal EUS,but also for access of retroperitoneal organs through posterior EUS guidance.

Farmer Income Differential in Regions

China＇s success in reform and opening up policy for twenty years is regarded as China＇s miracles in the world, whereas the income differential widening phenomenon has been the focus of the policymakers and researchers. This article researches 1994-2003 China＇s rural regions income differential and its decomposition. The method this paper used to measure the disparity is Gini Index. There are many ways to compute it, so the easiest way to decompose Gini Index-Matrix method is adopted. And based on it, farmer＇s income could be divided into wage income, fanning income, transfer income and property income according to its composition. The conclusion is that all of the indexes are between 0.2 and 0.3, at the comparatively average level. From the fluctuation trend, it increased from 1994 to 1995, while reduced from 1995 to 1996, fluctuated in 1997, and then diminished again. In general, farmer＇s regions income differential stays at comparatively average level, but it has the widening trend with time. Through decomposing Gini Index, wage income is the most important increasing factor, while fanning income is the reducing factor.

The Design and Implementation of the Network Interactive Virtual Experiment Platform for Electronic Information Courses

Experimental teaching plays a crucial role in the trainings of students＇ abilities to analyze and solve problems. Therefore, it is very necessary to continuously carry out experiment teaching reform, improve experiment teaching methods and means, and comprehensively open laboratories so as to provide students with more opportunities of doing experiments. To improve the teaching effect of electronic information experimental courses, a network interactive virtual experimental platform is developed. This platform allows students to finish the construction of all kinds of circuit structures and test virtual instruments in a simulation environment, upload experimental data and also interactively communicate with teachers through the lnternet. The platform can be applied to the teaching activities of related electronic information experimental courses, and also can be used as an aid teaching method for theory teachers. Therefore, it possesses a very good interactivity, and makes up the disadvantages of the experimental teaching in hardware environment, so that a feasible solution is provided for the opening of laboratories.

Remuneration Reforms in Public Sector： A Case of Russian Healthcare

In 2008 remuneration reform for public sector was introduced in Russia. Its main idea was to implement P4P principle well-known in business, including more flexible approach to wage setting. This paper presents an attempt to estimate an influence of the new remuneration system （NRS） on earnings level, inequalities and job motivation. The estimates are based on microdata of monitoring survey of healthcare economic problems conducted in Russia in 2009 and 2010. The extended specification of Mincer earning equation and probit-models were used. One could observe increasing wage rates and earning inequalities within healthcare institutions adopted NRS though worker＇s experience and regional economic differences remain significant wage determining factors. As it occurs, NRS is widely adopted by large regional and central hospitals while smaller health care institutions show less enthusiasm in implementing reform. Obviously, the larger institutions have more money and better educated administrative staff to introduce the new system. Those chief physicians who adopted NRS point out a positive correlation between earnings and individual input. At the same time, those committed to old principles of wage setting more often note declining job turnover. This latter result could possibly indicate negative personnel sorting, less productive workers tending to stay with employer who doesn＇t assess their performance. As concerns anticipated NRS results such as increasing motivation and quality of health services, the evidence is still ambiguous.

A study on the Application of the Activity Theory to Leaming Activity Design of MOOC in English Grammar Teaching

MOOC has become the topic of the education reform in recent years. From activity theory point of view, meaningful learning is an active, conscious, constructive, and socially medicated practice that includes reciprocal intention-action-reflection activities. This paper aims to try to give a MOOC learning activity design model in grammar teaching which tries to offer a reference for the MOOC teaching in grammar lesson.

Impact of Low-Impact Development Technologies from an Ecological Perspective in Different Residential Zones of the City of Atlanta, Georgia

Low-impact development （LID） technologies have a great potential to reduce water usage and stormwa- ter runoff and are therefore seen as sustainable improvements that can be made to traditional water infrastructure, These technologies include bioretention areas, rainwater capturing, and xeriscaping, all of which can be used in residential zones, Within the City of Atlanta, residential water usage accounts for 53% of the total water consumption; therefore, residential zones offer significant impact potential for the implementation of LID, This study analyzes the use of LID strategies within the different residen- tial zones of the City of Atlanta from an ecological perspective by drawing analogies to natural ecosys- tems, The analysis shows that these technologies, especially with the addition of a graywater system, work to improve the conventional residential water network based upon these ecological metrics, The higher metric values suggest greater parity with healthy, natural ecosystems.

Contribution of technology in language learning： A focus

The emerging technologies in various fields are affecting all walks of life including learning, teaching, education, etc.. The present learning in engineering colleges in the Indian scenario and their effect on general language proficiency is discussed. In the present era of job-oriented education how the accurate language learning and strengthening of skills facilitate one＇s career building is discussed here. The present paper focuses on independent, collaborative and supportive learning environment in the class to mould the reluctant learners. It also brings out the differences between conventional learning and technology assisted learning. In the midst of thousands of soft ware available, it is difficult for one to choose which is most suitable, to use friendly English learning process and which will be specific, more and more learners center and the need for such soft wares is given specific high light here. How the assessment should be carried through language modules is discussed here with a special attention to the learners and product assessment separately.

Pedestrianisation and Parking Policy as a Solution towards Green Transport Plan： A Case Study of Zagreb

Strategy of development trends in this moment and at this stage of the traffic comes from understanding the possibilities of the implementation of measures to raise the quality of movement and life in the city. It also includes planning and implementation of targeted interventions in the transport network or facilities. Mobility is a complex process in the area, starting from the departure from the apartment, using various means of transportation, a number of activities in different goals until to return to the location of the apartment. It thus requires extensive transport infrastructure in the form of walkways, bicycle paths, street and railway network. At the same time, reshaping of public transport facilities is needed in which the pedestrian, biker and green concept shall be the main elements of the new spatial conditions. Pedestrian zones development in the city means to create pedestrian areas and green islands in all the places in the city where possible. This process is essential for the transformation of public spaces, in particular, transport corridors. To accomplish the concept of the green transport plan, the need for change in the parking policy is emphasized. This should be an integral part of the city planning and should go hand in hand with the traffic policy.

Retro-Analysis of Rehabilitation of Existing Bridge over the River Cubiculo on Federal Highway BR-101, Brazil

This work describes the retro-analysis of design of rehabilitation of existing bridge over the river Cubiculo on BR-101 in Santa Catarina, Brazil, aiming to evaluate the intervention processes. In this way, it was verified that the project adopted for strengthening of the structure was not adequate to support the evolution of the maximum load over time and the overload due to widening, and it was also verified that the changes in the load factor do not comply with the update of the Brazilian Technical Standards ABNT： NBR-7188 [1]. The retro-analysis of the bridge rehabilitation project has demonstrated fundamental importance to avoid possible design failings, accidents, and reworks. Affirming that it is essential to comply with Standards and Technical Manuals on the elaboration of the rehabilitation project of structures and in choosing the type of intervention to the existing structure.