Objective: To study the relationship between the expression of Bax, Bcl 2 proteins, and apoptosis in radiation compound wound healing of rats. Methods: Apoptosis, Bax and Bcl 2 proteins were estimated by in situ termi...Objective: To study the relationship between the expression of Bax, Bcl 2 proteins, and apoptosis in radiation compound wound healing of rats. Methods: Apoptosis, Bax and Bcl 2 proteins were estimated by in situ terminal labeling (TUNEL) and immunohistochemical methods. Results: (1) Changes of the apoptosis in wound healing showed three typical characteristics: early occurrence, high frequency and delayed disappearance after radiation to rats when compared with those of simple wound group, which might be an important reason for radiation induced delayed wound healing. (2) The expression of Bax protein increased evidently with the increment of apoptosis and showed a good corresponding relationship with the apoptotic frequency in the process of wound healing. While the expression of Bcl 2 protein decreased obviously as the apoptosis reached a maximum and showed increasing tendency up to normal level when the apoptosis decreased distinctively. Conclusions: Bax and Bcl 2 proteins play an important role in the apoptotic regulation of radiation compound wound healing in rats.展开更多
基金ThisprojectwasgrantedbyUnit"95"Foundation (No .98Q0 86)andNational" 973"FoundationofChina (No .1 9990 542 0 4 )
文摘Objective: To study the relationship between the expression of Bax, Bcl 2 proteins, and apoptosis in radiation compound wound healing of rats. Methods: Apoptosis, Bax and Bcl 2 proteins were estimated by in situ terminal labeling (TUNEL) and immunohistochemical methods. Results: (1) Changes of the apoptosis in wound healing showed three typical characteristics: early occurrence, high frequency and delayed disappearance after radiation to rats when compared with those of simple wound group, which might be an important reason for radiation induced delayed wound healing. (2) The expression of Bax protein increased evidently with the increment of apoptosis and showed a good corresponding relationship with the apoptotic frequency in the process of wound healing. While the expression of Bcl 2 protein decreased obviously as the apoptosis reached a maximum and showed increasing tendency up to normal level when the apoptosis decreased distinctively. Conclusions: Bax and Bcl 2 proteins play an important role in the apoptotic regulation of radiation compound wound healing in rats.
