The distribution of ^(125)I recombinant E. coli L-asparaginase in tissues ororgans and the excretion in urine, feces and bile were studied with in vivo radioactive tracertechnique. The amount of radioactivity excreted...The distribution of ^(125)I recombinant E. coli L-asparaginase in tissues ororgans and the excretion in urine, feces and bile were studied with in vivo radioactive tracertechnique. The amount of radioactivity excreted in urine, feces and bile within 24 h afterintravenous administration of ^(125)I recombinant E. col L-asparaginase to rats was 68.95% ,4.44%and 5.36% of the dose respectively. ^(125)I recombinant E. coli L-asparaginase in plasma samples wasdetermined. The levels of structural intact molecule in plasma samples were evaluated by SDS-PAGEand bio-imaging analyzer system. Pharmacokinetic parameters were assessed with a model-dependentmethod. The concentration-time curves of recombinant E. coli L-asparaginase after intravenousinjection at 1 250 IU·kg^(-1), 2 500, IU·kg^(-1), 5 000 IU·kg^(-1) to rats were consistent withthe two-compartment model. The first and terminal elimination t_(1/2) were 0.52 ~ 0.63 h and 2.39 ~2.76 h respectively. AUC was linearly related to the doses. The results of distribution in tissuesor organs and excretion in urine suggested that the metabolites of the enzyme were cleared bymechanisms of urinary excretion. Pharmacokinetics parameters of recombinant E. coli L- asparaginasein rats are warranted for the design of future clinical trials.展开更多
^99Sr concentrations, resulting from the Chernobyl NPP accident, were determined in the salt lakes of the Crimea （Lakes Kiyatskoe, Kirleutskoe, Kizil-Yar, Bakalskoe and Donuzlav）, together with the redistribution be...^99Sr concentrations, resulting from the Chernobyl NPP accident, were determined in the salt lakes of the Crimea （Lakes Kiyatskoe, Kirleutskoe, Kizil-Yar, Bakalskoe and Donuzlav）, together with the redistribution between the components of the ecosystems. The content of mercury in the waters of the studied reservoirs was also established. Vertical distributions of natural radionuclide activities （^238U, ^232Th, ^226Ra, ^210pb, ^40K） and anthropogenic ^137Cs concentrations （as radiotracers） were determined in the bottom sediments of the Koyashskoe salt lake （located in the south-eastern Crimea） to evaluate the long- term dynamics and biogeochemical processes. Radiochemical and chemical analysis was undertaken and radiotracer and statistical methods were applied to the analytical data. The highest concentrations of ^99Sr in the water of Lake Kiyatskoe （350.5 and 98.0 Bq/m^3） and Lake Kirleutskoe （121.3 Bq/m^3） were due to the discharge of the Dnieper water from the North-Crimean Canal. The high content of mercury in Lake Kiyatskoe （363.2 ng/L） and in seawater near Lake Kizil-Yar （364 ng/L） exceeded the maximum permissible concentration （3.5 times the maximum）. Natural radionuclides provide the main contribution to the total radioactivity （artificial and natural combined） in the bottom sediments of Lake Koyashskoe. The significant concentration of ^210pb in the upper layer of bottom sediments of the lake indicates an active inflow of its parent radionuclide-gaseous ^222Rn from the lower layers of the bottom sediment. The average sedimentation rates in Lake Koyashskoe, determined using ^210pb and ^137Cs data, were 0.117 and 0.109 cm per year, respectively.展开更多
Y2000-62422-125 0105361采用放射性同位素示踪物对超薄键合绝缘体上硅硅片中铜扩散进行研究=Study of Cu diffusion in ultra thinbonded SOI wafers evaluated by using radioactive Isotopetracers[会,英]/Furihata,J.-I.& Nakan...Y2000-62422-125 0105361采用放射性同位素示踪物对超薄键合绝缘体上硅硅片中铜扩散进行研究=Study of Cu diffusion in ultra thinbonded SOI wafers evaluated by using radioactive Isotopetracers[会,英]/Furihata,J.-I.& Nakano,M.//1999IEEE International SOI Conference Proceedings.—125～156(EC)展开更多
基金ProjectsupportedbytheNationalNinth FivePlanKeyProjectFoundation No 96 90 2 0 1 2 5
文摘The distribution of ^(125)I recombinant E. coli L-asparaginase in tissues ororgans and the excretion in urine, feces and bile were studied with in vivo radioactive tracertechnique. The amount of radioactivity excreted in urine, feces and bile within 24 h afterintravenous administration of ^(125)I recombinant E. col L-asparaginase to rats was 68.95% ,4.44%and 5.36% of the dose respectively. ^(125)I recombinant E. coli L-asparaginase in plasma samples wasdetermined. The levels of structural intact molecule in plasma samples were evaluated by SDS-PAGEand bio-imaging analyzer system. Pharmacokinetic parameters were assessed with a model-dependentmethod. The concentration-time curves of recombinant E. coli L-asparaginase after intravenousinjection at 1 250 IU·kg^(-1), 2 500, IU·kg^(-1), 5 000 IU·kg^(-1) to rats were consistent withthe two-compartment model. The first and terminal elimination t_(1/2) were 0.52 ~ 0.63 h and 2.39 ~2.76 h respectively. AUC was linearly related to the doses. The results of distribution in tissuesor organs and excretion in urine suggested that the metabolites of the enzyme were cleared bymechanisms of urinary excretion. Pharmacokinetics parameters of recombinant E. coli L- asparaginasein rats are warranted for the design of future clinical trials.
文摘^99Sr concentrations, resulting from the Chernobyl NPP accident, were determined in the salt lakes of the Crimea （Lakes Kiyatskoe, Kirleutskoe, Kizil-Yar, Bakalskoe and Donuzlav）, together with the redistribution between the components of the ecosystems. The content of mercury in the waters of the studied reservoirs was also established. Vertical distributions of natural radionuclide activities （^238U, ^232Th, ^226Ra, ^210pb, ^40K） and anthropogenic ^137Cs concentrations （as radiotracers） were determined in the bottom sediments of the Koyashskoe salt lake （located in the south-eastern Crimea） to evaluate the long- term dynamics and biogeochemical processes. Radiochemical and chemical analysis was undertaken and radiotracer and statistical methods were applied to the analytical data. The highest concentrations of ^99Sr in the water of Lake Kiyatskoe （350.5 and 98.0 Bq/m^3） and Lake Kirleutskoe （121.3 Bq/m^3） were due to the discharge of the Dnieper water from the North-Crimean Canal. The high content of mercury in Lake Kiyatskoe （363.2 ng/L） and in seawater near Lake Kizil-Yar （364 ng/L） exceeded the maximum permissible concentration （3.5 times the maximum）. Natural radionuclides provide the main contribution to the total radioactivity （artificial and natural combined） in the bottom sediments of Lake Koyashskoe. The significant concentration of ^210pb in the upper layer of bottom sediments of the lake indicates an active inflow of its parent radionuclide-gaseous ^222Rn from the lower layers of the bottom sediment. The average sedimentation rates in Lake Koyashskoe, determined using ^210pb and ^137Cs data, were 0.117 and 0.109 cm per year, respectively.
文摘Y2000-62422-125 0105361采用放射性同位素示踪物对超薄键合绝缘体上硅硅片中铜扩散进行研究=Study of Cu diffusion in ultra thinbonded SOI wafers evaluated by using radioactive Isotopetracers[会,英]/Furihata,J.-I.& Nakano,M.//1999IEEE International SOI Conference Proceedings.—125～156(EC)
