Conventional radiotherapy remains to be one of the most useful treatments for cancer, but it is not the best strategy to maximize the effects on the tumors and minimize the damage to the surrounding tissues because of...Conventional radiotherapy remains to be one of the most useful treatments for cancer, but it is not the best strategy to maximize the effects on the tumors and minimize the damage to the surrounding tissues because of its physical and radiobiological characteristics. Synchrotrons represent a unique method for an innovative anti-cancer treatment due to the physical features (i.e. high fluence, tunable and collimated) of X-ray induced by synchrotron, so photon activation therapy and microbeam radiation treatment have been developed, but it is very imperative to understand the radiobiological mechanism of synchrotron radiation before we could transfer the strategy into the clinic. This paper is to summary the results of in vitro and in vivo experiments with synchrotron radiation, and review the advances of molecular and cellular radiobiological mechanism involved in synchrotron radiation. Since Shanghai Synchrotron Radiation Facility (SSRF) has produced the first synchrotron, it will provide the unique opportunity for the study of radiobiological effects of synchrotron radiation.展开更多
Objective: The aim of our study was to investigate the impact of abdominal hypo-fraction irradiation on liver damage in rats so as to provide a reference for its clinical application. Methods: A total of 100 Wistar ra...Objective: The aim of our study was to investigate the impact of abdominal hypo-fraction irradiation on liver damage in rats so as to provide a reference for its clinical application. Methods: A total of 100 Wistar rats were equally randomized to five groups as control, 4 Gy, 6 Gy, 8 Gy and 12 Gy group, and the corresponding fractionated doses were offered. Liver functions were examined at the 2nd, 4th, 6th, 8th and 10th week after irradiation. Morphological changes were observed by HE staining. Expressions of Bcl-2 and Bax were examined by immunohistochemical technique. Results: In all irradiation groups, hepatocellular swell, degeneration, necrosis and even hepatic fibrosis could be seen. The differences of the liver coefficient, Glutamyl pyruvic transaminase (GPT), Glutamyl oxaloacetic transaminase (GOT) were significant among the groups and different time points (F = 11.833-781.972, F = 20.857-264.692, P < 0.001). Expressions of Bcl-2 and Bax were significantly different between each group (F = 211.607, 116.577; P < 0. 001), and between each time point (F = 54.083, 68.749; P < 0. 001). Conclusion: Compare with conventional fraction, abdominal hypo-fraction irradiation may cause radiation damage to rat liver, being dose-and-time dependent. Up-regulation of activating apoptosis protein Bax and down-regulation of inhibiting apoptosis protein Bcl-2 may involve in the process.展开更多
基金Supported by Science and Technology Commission of Shanghai Municipality (NO.08411960600, NO.09JC1411900, NO.09411951100)
文摘Conventional radiotherapy remains to be one of the most useful treatments for cancer, but it is not the best strategy to maximize the effects on the tumors and minimize the damage to the surrounding tissues because of its physical and radiobiological characteristics. Synchrotrons represent a unique method for an innovative anti-cancer treatment due to the physical features (i.e. high fluence, tunable and collimated) of X-ray induced by synchrotron, so photon activation therapy and microbeam radiation treatment have been developed, but it is very imperative to understand the radiobiological mechanism of synchrotron radiation before we could transfer the strategy into the clinic. This paper is to summary the results of in vitro and in vivo experiments with synchrotron radiation, and review the advances of molecular and cellular radiobiological mechanism involved in synchrotron radiation. Since Shanghai Synchrotron Radiation Facility (SSRF) has produced the first synchrotron, it will provide the unique opportunity for the study of radiobiological effects of synchrotron radiation.
文摘Objective: The aim of our study was to investigate the impact of abdominal hypo-fraction irradiation on liver damage in rats so as to provide a reference for its clinical application. Methods: A total of 100 Wistar rats were equally randomized to five groups as control, 4 Gy, 6 Gy, 8 Gy and 12 Gy group, and the corresponding fractionated doses were offered. Liver functions were examined at the 2nd, 4th, 6th, 8th and 10th week after irradiation. Morphological changes were observed by HE staining. Expressions of Bcl-2 and Bax were examined by immunohistochemical technique. Results: In all irradiation groups, hepatocellular swell, degeneration, necrosis and even hepatic fibrosis could be seen. The differences of the liver coefficient, Glutamyl pyruvic transaminase (GPT), Glutamyl oxaloacetic transaminase (GOT) were significant among the groups and different time points (F = 11.833-781.972, F = 20.857-264.692, P < 0.001). Expressions of Bcl-2 and Bax were significantly different between each group (F = 211.607, 116.577; P < 0. 001), and between each time point (F = 54.083, 68.749; P < 0. 001). Conclusion: Compare with conventional fraction, abdominal hypo-fraction irradiation may cause radiation damage to rat liver, being dose-and-time dependent. Up-regulation of activating apoptosis protein Bax and down-regulation of inhibiting apoptosis protein Bcl-2 may involve in the process.
