Since the 1990s, the turnout in electoral processes has decreased, taking into account the Mexican context with its democratic transition, this decrease started after the 1994 federal election. The drop in turnout in ...Since the 1990s, the turnout in electoral processes has decreased, taking into account the Mexican context with its democratic transition, this decrease started after the 1994 federal election. The drop in turnout in federal elections had its lowest figure during the midterm elections of 2003, it picked up during the electoral process of 2009 and kept the same level of 2012. The change in the electoral turnout pattern for the federal elections in Mexico after 2009 can be explained as the combined effect of the electoral cycle and the concurrence between federal and local elections. Beginning from the hypothesis that there is a close link between institutions and contexts, the purpose of this study is to prove with the analysis of the electoral processes from 1997 to 2012, the effect of the electoral reforms--specifically the one in Article 116 of the Constitution in 2007-on turnout for federal elections of 2009 and 2012.展开更多
Dengue virus(DENV) nonstructural protein 1(NS1) is a highly conserved 46-kDa protein that contains 2 glycosylation sites(Asn-130 and Asn-207) and 12 conserved cysteine(Cys) residues. Here, we performed site-directed m...Dengue virus(DENV) nonstructural protein 1(NS1) is a highly conserved 46-kDa protein that contains 2 glycosylation sites(Asn-130 and Asn-207) and 12 conserved cysteine(Cys) residues. Here, we performed site-directed mutagenesis to generate systematic mutants of viral strain TSV01. The results of the subsequent analysis showed that an alanine substitution at the second N-linked glycan Asn-207 in NS1 delayed viral RNA synthesis, reduced virus plaque size, and weakened the cytopathic effect. Three mutants at Cys sites(Cys-4, Cys-55, Cys-291) and a C-terminal deletion(ΔC) mutant significantly impaired RNA synthesis, and consequently abolished viral growth, whereas alanine mutations at Asn-130 and Glu-173 resulted in phenotypes that were similar to the wild-type(WT) virus. Further analysis showed that the Asn-207 mutation slightly delayed viral replication. These results suggest that the three conserved disulfide bonds and the second N-linked glycan in NS1 are required for DENV-2 replication.展开更多
文摘Since the 1990s, the turnout in electoral processes has decreased, taking into account the Mexican context with its democratic transition, this decrease started after the 1994 federal election. The drop in turnout in federal elections had its lowest figure during the midterm elections of 2003, it picked up during the electoral process of 2009 and kept the same level of 2012. The change in the electoral turnout pattern for the federal elections in Mexico after 2009 can be explained as the combined effect of the electoral cycle and the concurrence between federal and local elections. Beginning from the hypothesis that there is a close link between institutions and contexts, the purpose of this study is to prove with the analysis of the electoral processes from 1997 to 2012, the effect of the electoral reforms--specifically the one in Article 116 of the Constitution in 2007-on turnout for federal elections of 2009 and 2012.
基金supported by Important National Science & Technology Specific Projects (2012ZX10004219, 2012ZX10004403)the National Natural Scientific Fund of China (81072675)the Wuhan Key Laboratory on Emerging Infectious Diseases and Biosafety
文摘Dengue virus(DENV) nonstructural protein 1(NS1) is a highly conserved 46-kDa protein that contains 2 glycosylation sites(Asn-130 and Asn-207) and 12 conserved cysteine(Cys) residues. Here, we performed site-directed mutagenesis to generate systematic mutants of viral strain TSV01. The results of the subsequent analysis showed that an alanine substitution at the second N-linked glycan Asn-207 in NS1 delayed viral RNA synthesis, reduced virus plaque size, and weakened the cytopathic effect. Three mutants at Cys sites(Cys-4, Cys-55, Cys-291) and a C-terminal deletion(ΔC) mutant significantly impaired RNA synthesis, and consequently abolished viral growth, whereas alanine mutations at Asn-130 and Glu-173 resulted in phenotypes that were similar to the wild-type(WT) virus. Further analysis showed that the Asn-207 mutation slightly delayed viral replication. These results suggest that the three conserved disulfide bonds and the second N-linked glycan in NS1 are required for DENV-2 replication.
