A highly sensitive SPE-liquid/liquid extraction RPLC method has been developed for the analysis of 6β-hydroxycortisol and cortisol in the urine of cancer patients. Methods： After SPE column purification and liquid-l...A highly sensitive SPE-liquid/liquid extraction RPLC method has been developed for the analysis of 6β-hydroxycortisol and cortisol in the urine of cancer patients. Methods： After SPE column purification and liquid-liquid extraction, the sample test solutions were analyzed with RPLC using a C18 analytical column. This improved analytical method has been validated for linearity, accuracy （recovery from urine）, repeatability （within-day and between-day precision）, specificity, sensitivity, and stability. This SPE-liquid/liquid extraction-RPLC is rapid, simple, accurate and reproducible. The technique is particularly useful for monitoring the CYP3A activity of cancer patients in clinical settings. The results are expressed as the ratio of 6β-hydroxycortisol to cortisol. Results： The CYP3A activity from a total of 153 samples was measured using this improved method. Considerable variation in the CYP3A activity of different cancer patients has been documented. Thus, personalized medical treatment based on the individual metabolic enzyme activity level is necessary. Conclusion： This new analytical method facilitates such individualized medical treatments.展开更多
By applying methods of investment appraisal, this paper offers a financial approach for determining the optimal level of segment-specific marketing activities and market development under the conditions of imperfect m...By applying methods of investment appraisal, this paper offers a financial approach for determining the optimal level of segment-specific marketing activities and market development under the conditions of imperfect markets and uncertainty. Within the scope of marketing planning and controlling, the model is suited to optimizing an enterprise＇s market activities and taking interdependencies between market segments, production, and investments into account. Applying duality theory of linear programming allows for identifying the income determinants and deriving formulas for a correct valuation by using （corrected） net present values （NPVs）. Under certain conditions, they can also be used to easily evaluate and financially interpret the effects of parameter changes. The author uses sensitivity analysis to support these findings and to obtain more information on the effects of these determinants.展开更多
We present a study on the retrieval sensitivity of the column-averaged dry-air mole fraction of CO2(XCO2) for the Chinese carbon dioxide observation satellite(TanSat) with a full physical forward model and the optimal...We present a study on the retrieval sensitivity of the column-averaged dry-air mole fraction of CO2(XCO2) for the Chinese carbon dioxide observation satellite(TanSat) with a full physical forward model and the optimal estimation technique. The forward model is based on the vector linearized discrete ordinate radiative transfer model(VLIDORT) and considers surface reflectance, gas absorption, and the scattering of air molecules, aerosol particles, and cloud particles. XCO2 retrieval errors from synthetic TanSat measurements show solar zenith angle(SZA), albedo dependence with values varying from 0.3 to 1 ppm for bright land surface in nadir mode and 2 to 8 ppm for dark surfaces like snow. The use of glint mode over dark oceans significantly improves the CO2 information retrieved. The aerosol type and profile are more important than the aerosol optical depth, and underestimation of aerosol plume height will introduce a bias of 1.5 ppm in XCO2. The systematic errors due to radiometric calibration are also estimated using a forward model simulation approach.展开更多
基金supported by the Shanghai Pharmaceutical Association
文摘A highly sensitive SPE-liquid/liquid extraction RPLC method has been developed for the analysis of 6β-hydroxycortisol and cortisol in the urine of cancer patients. Methods： After SPE column purification and liquid-liquid extraction, the sample test solutions were analyzed with RPLC using a C18 analytical column. This improved analytical method has been validated for linearity, accuracy （recovery from urine）, repeatability （within-day and between-day precision）, specificity, sensitivity, and stability. This SPE-liquid/liquid extraction-RPLC is rapid, simple, accurate and reproducible. The technique is particularly useful for monitoring the CYP3A activity of cancer patients in clinical settings. The results are expressed as the ratio of 6β-hydroxycortisol to cortisol. Results： The CYP3A activity from a total of 153 samples was measured using this improved method. Considerable variation in the CYP3A activity of different cancer patients has been documented. Thus, personalized medical treatment based on the individual metabolic enzyme activity level is necessary. Conclusion： This new analytical method facilitates such individualized medical treatments.
文摘By applying methods of investment appraisal, this paper offers a financial approach for determining the optimal level of segment-specific marketing activities and market development under the conditions of imperfect markets and uncertainty. Within the scope of marketing planning and controlling, the model is suited to optimizing an enterprise＇s market activities and taking interdependencies between market segments, production, and investments into account. Applying duality theory of linear programming allows for identifying the income determinants and deriving formulas for a correct valuation by using （corrected） net present values （NPVs）. Under certain conditions, they can also be used to easily evaluate and financially interpret the effects of parameter changes. The author uses sensitivity analysis to support these findings and to obtain more information on the effects of these determinants.
基金supported by the Strategic Priority Research Program- Climate Change: Carbon Budget and Relevant Issues (Grant No. XDA05040200)the National High-tech Research and Development Program of China (Grant No. 2011AA12A104)
文摘We present a study on the retrieval sensitivity of the column-averaged dry-air mole fraction of CO2(XCO2) for the Chinese carbon dioxide observation satellite(TanSat) with a full physical forward model and the optimal estimation technique. The forward model is based on the vector linearized discrete ordinate radiative transfer model(VLIDORT) and considers surface reflectance, gas absorption, and the scattering of air molecules, aerosol particles, and cloud particles. XCO2 retrieval errors from synthetic TanSat measurements show solar zenith angle(SZA), albedo dependence with values varying from 0.3 to 1 ppm for bright land surface in nadir mode and 2 to 8 ppm for dark surfaces like snow. The use of glint mode over dark oceans significantly improves the CO2 information retrieved. The aerosol type and profile are more important than the aerosol optical depth, and underestimation of aerosol plume height will introduce a bias of 1.5 ppm in XCO2. The systematic errors due to radiometric calibration are also estimated using a forward model simulation approach.
