Based on a series of aqua-planet and air–sea coupled experiments,the influence of unrealistic treatment of water substance in the Flexible Global Ocean–Atmosphere–Land System Model,spectral version 2（FGOALS-s2）,o...Based on a series of aqua-planet and air–sea coupled experiments,the influence of unrealistic treatment of water substance in the Flexible Global Ocean–Atmosphere–Land System Model,spectral version 2（FGOALS-s2）,on the model＇s climate sensitivity is investigated in this paper.Because the model does not adopt an explicit microphysics scheme,the detrained water substance from the convection scheme is converted back to the humidity.This procedure could lead to an additional increase of water vapor in the atmosphere,which could strengthen the model＇s climate sensitivity.Further sensitivity experiments confirm this deduction.After removing the water vapor converted from the detrained water substance,the water vapor reduced significantly in the upper troposphere and the high clouds also reduced.Quantitative calculations show that the water vapor reduced almost 10% of the total water vapor,and 50% at 150 h Pa,when the detrained water substance was removed,contributing to the 30% atmospheric surface temperature increase.This study calls for an explicit microphysics scheme to be introduced into the model in order to handle the detrained water vapor and thus improve the model＇s simulation skill.展开更多
Objective: To study the effects of transferred wild type p73α gene on the sensitivity to the chemotherapeutic agents and the growth of p53-null H1299 cells of human lung adenocarcinoma. Methods: The pcDNA3-HA-p73α p...Objective: To study the effects of transferred wild type p73α gene on the sensitivity to the chemotherapeutic agents and the growth of p53-null H1299 cells of human lung adenocarcinoma. Methods: The pcDNA3-HA-p73α plasmid was transferred into the cultured p53-null H1299 cells of human lung adenocarcinoma with the mediation of Dosper liposome; The cells resistant to G418 were selected. The expression of p73α gene in the cells was examined with Western blot. MTT assay was used to analyze the response of the transfected cells to cis-dichlorodiamine platinum (cDDP) and adriamycin (ADM). The rate of drug-induced apoptosis of the transfected cells was determined with flow cytometry and DNA fragmentation assay. The changes of the biological behaviors were observed with colony formation assay. Results: The transfected H1299 cells of human lung adenocarcinoma over-expressed p73α protein stably. MTT assay showed that the IC 50 values of cDDP and ADM were reduced by approximately 7 fold and 130 fold respectively in the transfected cells as compared with the untransfected ones. Lower concentration of the chemotherapeutic agents (1.25 μmol/L of cDDP and 0.05 μmol/L of ADM) could be employed to suppress markedly the growth of the transfected H1299 cells. The apoptotic rate induced by cDDP was increased from 10.1% to 38 4% (P<0.01) and that of ADM from 12.1% to 49.3% (P<0.01). The clonogenecity after the administration of chemotherapeutic agents was significantly lower in the transfected H1299 cells than in the parental cells (P<0.01). The sensitive enhancement ratios were 1.8 and 2.6 for cDDP and ADM respectively. Conclusion: The transfection of H1299 cells with wild type p73α gene results in an increase of the sensitivity of the cells to chemotherapeutic agents.展开更多
基金jointly supported by the National Basic Research Program of China[grant number 2014CB953904]the National Natural Science Foundation of China[grant numbers 41405091 and 91337110]+1 种基金the Open Projects of the Key Laboratory of Meteorological Disaster of the Ministry of Education[grant number KLME1405]the Strategic Leading Science Projects of the Chinese Academy of Sciences[grant number XDA11010402]
文摘Based on a series of aqua-planet and air–sea coupled experiments,the influence of unrealistic treatment of water substance in the Flexible Global Ocean–Atmosphere–Land System Model,spectral version 2（FGOALS-s2）,on the model＇s climate sensitivity is investigated in this paper.Because the model does not adopt an explicit microphysics scheme,the detrained water substance from the convection scheme is converted back to the humidity.This procedure could lead to an additional increase of water vapor in the atmosphere,which could strengthen the model＇s climate sensitivity.Further sensitivity experiments confirm this deduction.After removing the water vapor converted from the detrained water substance,the water vapor reduced significantly in the upper troposphere and the high clouds also reduced.Quantitative calculations show that the water vapor reduced almost 10% of the total water vapor,and 50% at 150 h Pa,when the detrained water substance was removed,contributing to the 30% atmospheric surface temperature increase.This study calls for an explicit microphysics scheme to be introduced into the model in order to handle the detrained water vapor and thus improve the model＇s simulation skill.
文摘Objective: To study the effects of transferred wild type p73α gene on the sensitivity to the chemotherapeutic agents and the growth of p53-null H1299 cells of human lung adenocarcinoma. Methods: The pcDNA3-HA-p73α plasmid was transferred into the cultured p53-null H1299 cells of human lung adenocarcinoma with the mediation of Dosper liposome; The cells resistant to G418 were selected. The expression of p73α gene in the cells was examined with Western blot. MTT assay was used to analyze the response of the transfected cells to cis-dichlorodiamine platinum (cDDP) and adriamycin (ADM). The rate of drug-induced apoptosis of the transfected cells was determined with flow cytometry and DNA fragmentation assay. The changes of the biological behaviors were observed with colony formation assay. Results: The transfected H1299 cells of human lung adenocarcinoma over-expressed p73α protein stably. MTT assay showed that the IC 50 values of cDDP and ADM were reduced by approximately 7 fold and 130 fold respectively in the transfected cells as compared with the untransfected ones. Lower concentration of the chemotherapeutic agents (1.25 μmol/L of cDDP and 0.05 μmol/L of ADM) could be employed to suppress markedly the growth of the transfected H1299 cells. The apoptotic rate induced by cDDP was increased from 10.1% to 38 4% (P<0.01) and that of ADM from 12.1% to 49.3% (P<0.01). The clonogenecity after the administration of chemotherapeutic agents was significantly lower in the transfected H1299 cells than in the parental cells (P<0.01). The sensitive enhancement ratios were 1.8 and 2.6 for cDDP and ADM respectively. Conclusion: The transfection of H1299 cells with wild type p73α gene results in an increase of the sensitivity of the cells to chemotherapeutic agents.
