A method of enhanced effect on calciumion and phosphate anion by resonance light-scattering turbidimetry(RLST) was studied in this paper.The optimum reaction conditions and the quantitative relationships of the intens...A method of enhanced effect on calciumion and phosphate anion by resonance light-scattering turbidimetry(RLST) was studied in this paper.The optimum reaction conditions and the quantitative relationships of the intensities of RLST to the concentration of phosphate anion were established.The RLS intensity at 515nm is max and proportional to the concentration of phosphate anion.The method is simple and rapid with a detection limit of 0.00608μg/mL and a linear rang of 0.02～3.0μg/mL.It was applied to the determination of organophorus pesticide residues with satisfactory results.展开更多
目的：本文对速率散射比浊法测定血清类风湿因子（RF）和抗链球菌溶血素O（ASO）进行方法学评价。方法：采用速率散射比浊法检测158例患者血清RF含量和96例患者血清ASO含量以及25例健康正常人血清RF和ASO含量，并同时与乳胶凝集法结果...目的：本文对速率散射比浊法测定血清类风湿因子（RF）和抗链球菌溶血素O（ASO）进行方法学评价。方法：采用速率散射比浊法检测158例患者血清RF含量和96例患者血清ASO含量以及25例健康正常人血清RF和ASO含量，并同时与乳胶凝集法结果对比。结果：批内、批间变异系数RF分别为1.83%和3.82%，ASO分别为2.76%和5.28%。定量检测具有满意的线性响应（RF r =0.987，ASO r =0.995）。免疫比浊法ASO阳性率为16.6%，乳胶法为8.3%，RF比浊法阳性率为24.1%，乳胶法为18.4%，比浊法阳性率明显高于乳胶法。测定25例健康正常人，RF均小于20IU/ml，ASO均值为70.7±55.6IU/ml。结论：本方法的重复性、 准确性、稳定性、特异性均较好，脂血、溶血、黄疸无明显影响，具有较高的临床应用价值。展开更多
文摘A method of enhanced effect on calciumion and phosphate anion by resonance light-scattering turbidimetry(RLST) was studied in this paper.The optimum reaction conditions and the quantitative relationships of the intensities of RLST to the concentration of phosphate anion were established.The RLS intensity at 515nm is max and proportional to the concentration of phosphate anion.The method is simple and rapid with a detection limit of 0.00608μg/mL and a linear rang of 0.02～3.0μg/mL.It was applied to the determination of organophorus pesticide residues with satisfactory results.
文摘目的：本文对速率散射比浊法测定血清类风湿因子（RF）和抗链球菌溶血素O（ASO）进行方法学评价。方法：采用速率散射比浊法检测158例患者血清RF含量和96例患者血清ASO含量以及25例健康正常人血清RF和ASO含量，并同时与乳胶凝集法结果对比。结果：批内、批间变异系数RF分别为1.83%和3.82%，ASO分别为2.76%和5.28%。定量检测具有满意的线性响应（RF r =0.987，ASO r =0.995）。免疫比浊法ASO阳性率为16.6%，乳胶法为8.3%，RF比浊法阳性率为24.1%，乳胶法为18.4%，比浊法阳性率明显高于乳胶法。测定25例健康正常人，RF均小于20IU/ml，ASO均值为70.7±55.6IU/ml。结论：本方法的重复性、 准确性、稳定性、特异性均较好，脂血、溶血、黄疸无明显影响，具有较高的临床应用价值。
