AIM： To study the association between Interleukin-1 （IL-1） and tumor necrosis factor （TNF）-α polymorphisms, infection by Helicobacter pylori （H pylori） and the development of gastrointestinal diseases.METHODS...AIM： To study the association between Interleukin-1 （IL-1） and tumor necrosis factor （TNF）-α polymorphisms, infection by Helicobacter pylori （H pylori） and the development of gastrointestinal diseases.METHODS： Genomic DNA was extracted from the peripheral blood of 177 patients with various gastrointestinal diseases and from 100 healthy volunteers. The polymorphisms in IL-1β and TNF-α genes were analyzed using the polymerase chain reactionrestriction fragment length polymorphism method （PCRRFLP） and those from IL-1RN with PCR. The presence of infection due to H pylori and the presence of the CagA toxin were detected by serology. The histopathological parameters in the gastric biopsies of the patients were according to the Sydney classification.RESULTS： A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL- 1RN＊2 was more frequent among patients with gastric ulcers and adenocarcinoma. Carriers of the allele IL- RN＊2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma, as well as a higher degree of inflammation and neutrophilic activity in the gastricCONCLUSION： Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma.展开更多
The peer-to-peer（P2P） file-sharing network as a vehicle of disseminating files has become very popular. The appearance of dozens of kinds of passive worms on this network has, however, made it unsecured. This proble...The peer-to-peer（P2P） file-sharing network as a vehicle of disseminating files has become very popular. The appearance of dozens of kinds of passive worms on this network has, however, made it unsecured. This problem has been paid attention and a few of models for passive worm propagation has been presented. Unfortunately, the dynamic properties of this network are ignored in these models. Given the fact, the characteristics of both this network and the passive worm are identified, and on this basis a new mathematical model of passive worm propagation on the P2P network is presented in applying epidemiology in this paper. Note that the dynamic properties of this network are considered in the presented model. The model has been validated by large scale simulation experiments, which demonstrates that the presented model may be used for analyzing the behaviors of passive worms and predicting the trend of their propagation.展开更多
基金Supported by Secretaria Executiva de Ciência,Tecnologiae Meio Ambiente-SECTAM and Coordenao de Aperfeioamento de Pessoal de Nível Superior-CAPES
文摘AIM： To study the association between Interleukin-1 （IL-1） and tumor necrosis factor （TNF）-α polymorphisms, infection by Helicobacter pylori （H pylori） and the development of gastrointestinal diseases.METHODS： Genomic DNA was extracted from the peripheral blood of 177 patients with various gastrointestinal diseases and from 100 healthy volunteers. The polymorphisms in IL-1β and TNF-α genes were analyzed using the polymerase chain reactionrestriction fragment length polymorphism method （PCRRFLP） and those from IL-1RN with PCR. The presence of infection due to H pylori and the presence of the CagA toxin were detected by serology. The histopathological parameters in the gastric biopsies of the patients were according to the Sydney classification.RESULTS： A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL- 1RN＊2 was more frequent among patients with gastric ulcers and adenocarcinoma. Carriers of the allele IL- RN＊2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma, as well as a higher degree of inflammation and neutrophilic activity in the gastricCONCLUSION： Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma.
文摘The peer-to-peer（P2P） file-sharing network as a vehicle of disseminating files has become very popular. The appearance of dozens of kinds of passive worms on this network has, however, made it unsecured. This problem has been paid attention and a few of models for passive worm propagation has been presented. Unfortunately, the dynamic properties of this network are ignored in these models. Given the fact, the characteristics of both this network and the passive worm are identified, and on this basis a new mathematical model of passive worm propagation on the P2P network is presented in applying epidemiology in this paper. Note that the dynamic properties of this network are considered in the presented model. The model has been validated by large scale simulation experiments, which demonstrates that the presented model may be used for analyzing the behaviors of passive worms and predicting the trend of their propagation.
