A new,simple,rapid microbial assay was put forward for the detection of chloramphenicol(CAP)residues in Turbot muscles using luminescent bacteria DB(Photobacterium phosphoreum)as test organ-ism.By the luminescence inh...A new,simple,rapid microbial assay was put forward for the detection of chloramphenicol(CAP)residues in Turbot muscles using luminescent bacteria DB(Photobacterium phosphoreum)as test organ-ism.By the luminescence inhibition of DB,the concentrations of CAP were determined.CAP spiked inTurbot muscle tissues ranging from 0.5 to 100μg/kg was extracted using ethyl acetate and hexane.Themethod had a good recovery of 93.69% with a minimum detection limit of 0.65μg/kg.It can be per-formed within 30min following simple extraction.The method therefore proved to be advantageous overchromatographic procedures as it was inexpensive,quite sensitive and can be adopted for rapid screeningof CAP in Turbot tissues.展开更多
OBJECTIVE: To screen and summarize evaluation indexes for symptom changes in Chinese medicine(CM).METHODS: A case database was established based on information from subsequent patient visits from the case records of f...OBJECTIVE: To screen and summarize evaluation indexes for symptom changes in Chinese medicine(CM).METHODS: A case database was established based on information from subsequent patient visits from the case records of famous doctors since 1866.Symptom change descriptions in the database were regarded as research materials. The evaluation indexes of the symptom changes were screened and summarized.RESULTS: In total, 243 evaluation indexes for 256symptoms were summarized. Among them, common symptoms were frequency, quantity, degree,mobility, color, and correlation with fatigue.CONCLUSION: There are many aspects to evaluating the changes in a symptom. Some symptoms occur with other simultaneous symptoms. The alleviation or aggravation of simultaneous symptoms could reflect the corresponding changes in a symptom. The changes of inducing factors are important to judging changes in symptoms.展开更多
基金Supported by the National Natural Science Foundation of China ( No. 30500384)the High Technology Research and Development Programme of China (No. 2006AA09Z431)
文摘A new,simple,rapid microbial assay was put forward for the detection of chloramphenicol(CAP)residues in Turbot muscles using luminescent bacteria DB(Photobacterium phosphoreum)as test organ-ism.By the luminescence inhibition of DB,the concentrations of CAP were determined.CAP spiked inTurbot muscle tissues ranging from 0.5 to 100μg/kg was extracted using ethyl acetate and hexane.Themethod had a good recovery of 93.69% with a minimum detection limit of 0.65μg/kg.It can be per-formed within 30min following simple extraction.The method therefore proved to be advantageous overchromatographic procedures as it was inexpensive,quite sensitive and can be adopted for rapid screeningof CAP in Turbot tissues.
基金Supported by the National Natural Science Foundation of China (the Diagnostic Points of General Syndrome Factors on the Symptomatic Units and their Clinical Characteristics,No.81001500)National Program of Science and Technology Development (the Research on Sweating Pattern Recognition of Chinese Medicine and the Development of High-Precision Detection Device,No.2012 BAI25B07)
文摘OBJECTIVE: To screen and summarize evaluation indexes for symptom changes in Chinese medicine(CM).METHODS: A case database was established based on information from subsequent patient visits from the case records of famous doctors since 1866.Symptom change descriptions in the database were regarded as research materials. The evaluation indexes of the symptom changes were screened and summarized.RESULTS: In total, 243 evaluation indexes for 256symptoms were summarized. Among them, common symptoms were frequency, quantity, degree,mobility, color, and correlation with fatigue.CONCLUSION: There are many aspects to evaluating the changes in a symptom. Some symptoms occur with other simultaneous symptoms. The alleviation or aggravation of simultaneous symptoms could reflect the corresponding changes in a symptom. The changes of inducing factors are important to judging changes in symptoms.
