The floral-organ-number mutant fon(t) was firstly discovered in the progeny of a cross between a diploid (Chunjiang 683) and a haploid (SARⅣ/-620-A) rice cultivar. The fon(t) mutant showed normal vegetative d...The floral-organ-number mutant fon(t) was firstly discovered in the progeny of a cross between a diploid (Chunjiang 683) and a haploid (SARⅣ/-620-A) rice cultivar. The fon(t) mutant showed normal vegetative development and produced normal inflorescence structures. Difference between the mutant and the wild type was observed when the stamen primordia began to form. The mature flowers offon(t) mutant showed open-hull phenotypes, which resulted in the exposure of stamens and stigmas. Normally, a single fon(t) floret consisted of six to nine stamens and one or two pistils. In addition, stamen/pistil-like structures and bulged tissues near ovaries were also observed in a few fon(t) florets. But homeotic transformation of lodicules into palea/lemma-like organs was observed almost in all the open-hull florets. The phenotypes offon(t) flowers also suggested thatfon(t) gene might affect flower organ identity in the inner whorls. Genetic analysis showed that thefon(t) mutant was controlled by a single recessive gene.展开更多
A genetic linkage map with 89 SSR marker loci was constructed based on a maize (Zea mays L.) population consisting of 184 F-2 individuals from the cross, Huangzao 4 X Ye 107. The 184 F-3 families were evaluated in the...A genetic linkage map with 89 SSR marker loci was constructed based on a maize (Zea mays L.) population consisting of 184 F-2 individuals from the cross, Huangzao 4 X Ye 107. The 184 F-3 families were evaluated in the field under well-watered and drought-stressed regimes in Shanxi Province of China. The objectives of the study were to identify genetic segments responsible for the expression of anthesis-silking interval (ASI), ear setting and grain yield, and to examine if the quantitative trait loci (QTLs) for ASI or yield components can be used in marker-assisted selection (MAS) to improve grain yield under drought conditions. Results showed that under well-watered and drought-stressed regimes, three and two QTLs involved in the expression of ASI were detected on chromosomes 1, 2 and 3, and 2 and 5, respectively. Under well-watered regime, two QTLs for ear setting were detected on chromosomes 3 and 6, explaining about 19.9% of the phenotypic variance, and displayed additive and partial dominant effects, respectively. Under drought-stressed condition, four QTLs for ear setting were detected on chromosomes 3, 7 and 10, which were responsible for interpreting 60.4% of the phenotypic variance, and showed dominant or partial dominant effects. Under well-watered condition, four QTLs controlling grain yield were identified on chromosomes 3, 6 and 7, while five QTLs were identified under drought stress on chromosomes 1, 2, 4 and 8. The gene action was of additive or partial dominant effects, and each QTL could explain 7.3% to 22.0% of the phenotypic variance, respectively. Under drought conditions, ASI and ear setting percentage were highly correlated with grain yield, which can be used as secondary traits for grain yield selection. Based on linked markers detected and gene action analyzed, an MAS strategy for yield improvement under drought condition could be established, which consists of QTLs contributing to decreased ASI and to increased ear setting and grain yield, respectively.展开更多
[ Objective] The aim of this study was to solve the problem of photographing small flowers and fruits with a common digital camera in field. [Method] Firstly, the photographic mode of a common digital camera was set ...[ Objective] The aim of this study was to solve the problem of photographing small flowers and fruits with a common digital camera in field. [Method] Firstly, the photographic mode of a common digital camera was set as "macro" (or "soft snap" ) and "flash off". Secondly, the small flower or fruit was magnified with a magnifying glass, and then the magnified flower or fruit was automatically focused with the digital camera lens. After automatic focusing, the shutter button was pressed and the photograph finished. [Result] With this method, the clear inflo- rescence or floral photographs of Eragrostis pilosa ( Gramineae ) , Bidens biternata (Compositae) and Tribulus terrester (Zygophllaceae) were obtained in field. [ Conclusion] The optical system of a digital camera has been changed by the magnifying glass, which improves the photographic function of a common digital camera to small flowers and fruits, and also easily gets their clear photographs.展开更多
[Objective] Pollen mother cell miosis and male gametophyte development of pumpkin were observed in this study, to provide some cytological basis for pumpkin anther or microspore culture. [Method] Ehrlich's hematoxyli...[Objective] Pollen mother cell miosis and male gametophyte development of pumpkin were observed in this study, to provide some cytological basis for pumpkin anther or microspore culture. [Method] Ehrlich's hematoxylin staining-methyl salicylate clearing technique was used for observation and research of the variation of cell structure and chromosomal behavior during pollen mother cell miosis and male gametophyte development of ‘Tianhong' pumpkin. [Result] The meiosis in pollen moth- er cells of pumpkin was simultaneous cytokinesis. In the process of nuclear division, nuclear membrane and nucleolus of pumpkin pollen mother cells gradually disappeared in the metaphase I and reappeared in telophase I , phragmoplast formed between the two generated crescent-shaped nuclei without cell wall, the phragmoplast gradually disappeared in the metaphase II and reappeared in telophase II. Phragmoplast spread outward from the center of spindle during the second division was connected with that formed on the central interface of two nuclei during the first division, cell wall of microspores generated from periphery to center. Most of the tetrads contained four sub-cells while a few contained extra small cells. During the period of uniuclete microspore at periphery, the single nucleolus split into 2-3 or more small nucleoli, mature pollen grain was two-celled. Mononucleate pollen cells were mostly appeared in the flower buds with length of 1.0-2.0 cm, which could be used as an important indicator to collect materials for anther or microspore culture. [Conclusion] This study laid the foundation for research of the cytogenetics of pumpkin.展开更多
Haihua Islands is a large artificial island in Danzhou, Hainan. The construction of Haihua Islands changes the hYdrodynamic environment of Yangpu waters, and further affects its morphological change. Delft3D is used t...Haihua Islands is a large artificial island in Danzhou, Hainan. The construction of Haihua Islands changes the hYdrodynamic environment of Yangpu waters, and further affects its morphological change. Delft3D is used to set up a two dimensional nested hydrodynamic and sediment model for Yangpu waters in this paper, and this paper focuses on simulating the velocity and morphological change due to the construction of Haihua Islands after the verification of the model. The seabed deposition is small because of low suspended sediment concentration and less sand source near Yangpu waters. The bed level erodes in the south area of Xiaochan Reef and the Yangpu channel due to the velocity increase in the area.展开更多
In this study, Ficus carica L. ‘Masui Dauphine' was used as the experi- mental material to investigate the effects of explant type, basal medium, hormone types and concentrations on in vitro rapid propagation of‘Ma...In this study, Ficus carica L. ‘Masui Dauphine' was used as the experi- mental material to investigate the effects of explant type, basal medium, hormone types and concentrations on in vitro rapid propagation of‘Masui Dauphine'. Accord- ing to the results, the most suitable explants for in vitro rapid propagation of ‘Masui Dauphine' were axillary buds and the best medium was modified MS + 1.0 mg/L 6-BA + 0. 05 mg/L NAA + 1.0 mg/L GA3 + 20 mg/L sucrose + 7 mg/L agar, pH 5.8. This study provided scientific basis for barge-scale cultivation of‘Masui Dauphine '.展开更多
基金This work was supported by the Program for Changjiang Scholars and Innovative Research Team in University (No. IRTO453)
文摘The floral-organ-number mutant fon(t) was firstly discovered in the progeny of a cross between a diploid (Chunjiang 683) and a haploid (SARⅣ/-620-A) rice cultivar. The fon(t) mutant showed normal vegetative development and produced normal inflorescence structures. Difference between the mutant and the wild type was observed when the stamen primordia began to form. The mature flowers offon(t) mutant showed open-hull phenotypes, which resulted in the exposure of stamens and stigmas. Normally, a single fon(t) floret consisted of six to nine stamens and one or two pistils. In addition, stamen/pistil-like structures and bulged tissues near ovaries were also observed in a few fon(t) florets. But homeotic transformation of lodicules into palea/lemma-like organs was observed almost in all the open-hull florets. The phenotypes offon(t) flowers also suggested thatfon(t) gene might affect flower organ identity in the inner whorls. Genetic analysis showed that thefon(t) mutant was controlled by a single recessive gene.
文摘A genetic linkage map with 89 SSR marker loci was constructed based on a maize (Zea mays L.) population consisting of 184 F-2 individuals from the cross, Huangzao 4 X Ye 107. The 184 F-3 families were evaluated in the field under well-watered and drought-stressed regimes in Shanxi Province of China. The objectives of the study were to identify genetic segments responsible for the expression of anthesis-silking interval (ASI), ear setting and grain yield, and to examine if the quantitative trait loci (QTLs) for ASI or yield components can be used in marker-assisted selection (MAS) to improve grain yield under drought conditions. Results showed that under well-watered and drought-stressed regimes, three and two QTLs involved in the expression of ASI were detected on chromosomes 1, 2 and 3, and 2 and 5, respectively. Under well-watered regime, two QTLs for ear setting were detected on chromosomes 3 and 6, explaining about 19.9% of the phenotypic variance, and displayed additive and partial dominant effects, respectively. Under drought-stressed condition, four QTLs for ear setting were detected on chromosomes 3, 7 and 10, which were responsible for interpreting 60.4% of the phenotypic variance, and showed dominant or partial dominant effects. Under well-watered condition, four QTLs controlling grain yield were identified on chromosomes 3, 6 and 7, while five QTLs were identified under drought stress on chromosomes 1, 2, 4 and 8. The gene action was of additive or partial dominant effects, and each QTL could explain 7.3% to 22.0% of the phenotypic variance, respectively. Under drought conditions, ASI and ear setting percentage were highly correlated with grain yield, which can be used as secondary traits for grain yield selection. Based on linked markers detected and gene action analyzed, an MAS strategy for yield improvement under drought condition could be established, which consists of QTLs contributing to decreased ASI and to increased ear setting and grain yield, respectively.
基金Supported by National Natural Science Foundation(30770124)~~
文摘[ Objective] The aim of this study was to solve the problem of photographing small flowers and fruits with a common digital camera in field. [Method] Firstly, the photographic mode of a common digital camera was set as "macro" (or "soft snap" ) and "flash off". Secondly, the small flower or fruit was magnified with a magnifying glass, and then the magnified flower or fruit was automatically focused with the digital camera lens. After automatic focusing, the shutter button was pressed and the photograph finished. [Result] With this method, the clear inflo- rescence or floral photographs of Eragrostis pilosa ( Gramineae ) , Bidens biternata (Compositae) and Tribulus terrester (Zygophllaceae) were obtained in field. [ Conclusion] The optical system of a digital camera has been changed by the magnifying glass, which improves the photographic function of a common digital camera to small flowers and fruits, and also easily gets their clear photographs.
基金Supported by Project of Jiangsu Provincial Department of Education (JHZD06-7)Qing Lan Project of Colleges and Universities in Jiangsu Province (2008 No.30)~~
文摘[Objective] Pollen mother cell miosis and male gametophyte development of pumpkin were observed in this study, to provide some cytological basis for pumpkin anther or microspore culture. [Method] Ehrlich's hematoxylin staining-methyl salicylate clearing technique was used for observation and research of the variation of cell structure and chromosomal behavior during pollen mother cell miosis and male gametophyte development of ‘Tianhong' pumpkin. [Result] The meiosis in pollen moth- er cells of pumpkin was simultaneous cytokinesis. In the process of nuclear division, nuclear membrane and nucleolus of pumpkin pollen mother cells gradually disappeared in the metaphase I and reappeared in telophase I , phragmoplast formed between the two generated crescent-shaped nuclei without cell wall, the phragmoplast gradually disappeared in the metaphase II and reappeared in telophase II. Phragmoplast spread outward from the center of spindle during the second division was connected with that formed on the central interface of two nuclei during the first division, cell wall of microspores generated from periphery to center. Most of the tetrads contained four sub-cells while a few contained extra small cells. During the period of uniuclete microspore at periphery, the single nucleolus split into 2-3 or more small nucleoli, mature pollen grain was two-celled. Mononucleate pollen cells were mostly appeared in the flower buds with length of 1.0-2.0 cm, which could be used as an important indicator to collect materials for anther or microspore culture. [Conclusion] This study laid the foundation for research of the cytogenetics of pumpkin.
文摘Haihua Islands is a large artificial island in Danzhou, Hainan. The construction of Haihua Islands changes the hYdrodynamic environment of Yangpu waters, and further affects its morphological change. Delft3D is used to set up a two dimensional nested hydrodynamic and sediment model for Yangpu waters in this paper, and this paper focuses on simulating the velocity and morphological change due to the construction of Haihua Islands after the verification of the model. The seabed deposition is small because of low suspended sediment concentration and less sand source near Yangpu waters. The bed level erodes in the south area of Xiaochan Reef and the Yangpu channel due to the velocity increase in the area.
文摘In this study, Ficus carica L. ‘Masui Dauphine' was used as the experi- mental material to investigate the effects of explant type, basal medium, hormone types and concentrations on in vitro rapid propagation of‘Masui Dauphine'. Accord- ing to the results, the most suitable explants for in vitro rapid propagation of ‘Masui Dauphine' were axillary buds and the best medium was modified MS + 1.0 mg/L 6-BA + 0. 05 mg/L NAA + 1.0 mg/L GA3 + 20 mg/L sucrose + 7 mg/L agar, pH 5.8. This study provided scientific basis for barge-scale cultivation of‘Masui Dauphine '.