According to overall mean square root of weighted deviation, a significant method of selecting coal mine production technique strategy has been put forward in this paper. In the given example, an index system of evalu...According to overall mean square root of weighted deviation, a significant method of selecting coal mine production technique strategy has been put forward in this paper. In the given example, an index system of evaluating different mining methods has also been provided, which plays a guiding effect in production of coal mine.展开更多
Objective：To establish the polytransgenic mice expressing the human HT and complement regulatory proteins （CRPs） and discuss their ability to resist the hyperacute rejection （HAR） and delayed xenograft rejection ...Objective：To establish the polytransgenic mice expressing the human HT and complement regulatory proteins （CRPs） and discuss their ability to resist the hyperacute rejection （HAR） and delayed xenograft rejection （DXR） of heterogenic transplantation. Methods ：Transgenic mice were produced by microinjection to construct gene for human HT, delay acceleration factor （DAF） and/or CD59 into the male pronucleus of zygote. PCR and Southern blot were used to screen the positive trarisgenic mice. Flow cytometry （FCM） was used to detect the expression of HT, ct-Gal and DAF or CD59 on the PBMCs of transgenic mice. The survival time and function of the heart of transgenic mice were determined by a modified Langendorff cardiac perfusion apparatus： The change of proteinosis on IgM,IgG, C3c and C9 from different cardiac vascular iendothelial cells of transgenic mice were detected by immunohistochemistry. Results：HT, DAF or CD59 were highly expressed on the positive transgenic mice by FCM. The deposition of IgM,IgG,C3c or C9 in the cardiac vascular endothelial cells of the positive transgenic mice were de- creased. The survival time and function of the heart of the co-transgenic mice with AB serum perfusion were significantly longer and higher than that of the single HT positive transgenic mice（P 〈0.05）. Conclusion ：The mice co-expressing HT/DAF or HT/CD59 could resist the HAR,which was better than those expressing HT alone. It is feasible to use HT and CRPs co-transgenic methods to resist the HAR and DXR.展开更多
文摘According to overall mean square root of weighted deviation, a significant method of selecting coal mine production technique strategy has been put forward in this paper. In the given example, an index system of evaluating different mining methods has also been provided, which plays a guiding effect in production of coal mine.
基金Tianjin Municipal Science and Technology CommissionGrant number:043803411
文摘Objective：To establish the polytransgenic mice expressing the human HT and complement regulatory proteins （CRPs） and discuss their ability to resist the hyperacute rejection （HAR） and delayed xenograft rejection （DXR） of heterogenic transplantation. Methods ：Transgenic mice were produced by microinjection to construct gene for human HT, delay acceleration factor （DAF） and/or CD59 into the male pronucleus of zygote. PCR and Southern blot were used to screen the positive trarisgenic mice. Flow cytometry （FCM） was used to detect the expression of HT, ct-Gal and DAF or CD59 on the PBMCs of transgenic mice. The survival time and function of the heart of transgenic mice were determined by a modified Langendorff cardiac perfusion apparatus： The change of proteinosis on IgM,IgG, C3c and C9 from different cardiac vascular iendothelial cells of transgenic mice were detected by immunohistochemistry. Results：HT, DAF or CD59 were highly expressed on the positive transgenic mice by FCM. The deposition of IgM,IgG,C3c or C9 in the cardiac vascular endothelial cells of the positive transgenic mice were de- creased. The survival time and function of the heart of the co-transgenic mice with AB serum perfusion were significantly longer and higher than that of the single HT positive transgenic mice（P 〈0.05）. Conclusion ：The mice co-expressing HT/DAF or HT/CD59 could resist the HAR,which was better than those expressing HT alone. It is feasible to use HT and CRPs co-transgenic methods to resist the HAR and DXR.
