In this study, the novel RGD-modified stabilized cationic liposomes were developed as the delivery vehicle for siRNA targeting human MDR1 gene. The complex of cationic liposomes and siRNA, RGD-Lipo-siRNA, was prepared...In this study, the novel RGD-modified stabilized cationic liposomes were developed as the delivery vehicle for siRNA targeting human MDR1 gene. The complex of cationic liposomes and siRNA, RGD-Lipo-siRNA, was prepared with a narrow size distribution below 200 nm. It was shown that the encapsulated siRNA in the liposomes could be effectively protected from serum degradation. Also, enhanced cell binding and intracellular uptake of siRNA in the doxorubicin-resistant human ova- rian cancer cell lines SKOV3/A were found in RGD-Lipo-siRNA preparation as compared to that of unmodified cationic lipsomes （Lipo-siRNA）. Using the post-insertion method for RGD modification, lysosome release of siRNA in pRGD-Lipo-siRNA was improved. From flow cytometry, significant increase of doxorubicin accumulation was observed in the SKOV3/A cells treated with pRGD-Lipo-siRNA targeting human MDR1 gene. In vitro cytotoxicity assay showed that the significant cell growth inhibition was achieved in the SKOV3/A cells after treating with the combined use of siRNA and doxorubicin. In conclusions, postinserted RGD modified lipoplex, pRGD-Lipo-siRNA, was successfully used for siRNA transfection and achieved drug resistance reversal in human ovarian cancer SKOV3/A （doxorubicin-resistant） cells. It suggested that this liposomes might be a potential vehicle for siRNA delivery in vivo.展开更多
In this paper, we conduct analysis on the optimization approaches of contemporary piano education system from the perspective of music knowledge integration. In the process of the piano teaching, teacher and school th...In this paper, we conduct analysis on the optimization approaches of contemporary piano education system from the perspective of music knowledge integration. In the process of the piano teaching, teacher and school the training of musicianship as the key point of teaching. This is because the skill can through student plays to display directly, appraises the student performance technique quality an important criterion. However, the student has the excellent adept technique merely also by far insufficient, but also needs the high artistic aesthetic ability, not only need be able to appreciate the piano performance, but must be able to reveal the artistic aesthetic feature in the process of piano performance. Piano education as one of the ways of music education, and other subjects, there is the subject of the universal education as its educational ideas, educational ideas and teaching methods, a direct impact on the generation after generation of the comprehensive quality of education. Under this basis, we propose the new idea on the issues that will be beneficial.展开更多
基金National Natural Science Foundation of China(Grant No.30701056)Foundation of MOST(973 Program,Grant No.2007CB935801)+1 种基金Beijing Natural Science Foundation of China(Grant No.7083112)Doctoral Foundation of Ministry of Education of China(Grant No.20070001813).
文摘In this study, the novel RGD-modified stabilized cationic liposomes were developed as the delivery vehicle for siRNA targeting human MDR1 gene. The complex of cationic liposomes and siRNA, RGD-Lipo-siRNA, was prepared with a narrow size distribution below 200 nm. It was shown that the encapsulated siRNA in the liposomes could be effectively protected from serum degradation. Also, enhanced cell binding and intracellular uptake of siRNA in the doxorubicin-resistant human ova- rian cancer cell lines SKOV3/A were found in RGD-Lipo-siRNA preparation as compared to that of unmodified cationic lipsomes （Lipo-siRNA）. Using the post-insertion method for RGD modification, lysosome release of siRNA in pRGD-Lipo-siRNA was improved. From flow cytometry, significant increase of doxorubicin accumulation was observed in the SKOV3/A cells treated with pRGD-Lipo-siRNA targeting human MDR1 gene. In vitro cytotoxicity assay showed that the significant cell growth inhibition was achieved in the SKOV3/A cells after treating with the combined use of siRNA and doxorubicin. In conclusions, postinserted RGD modified lipoplex, pRGD-Lipo-siRNA, was successfully used for siRNA transfection and achieved drug resistance reversal in human ovarian cancer SKOV3/A （doxorubicin-resistant） cells. It suggested that this liposomes might be a potential vehicle for siRNA delivery in vivo.
文摘In this paper, we conduct analysis on the optimization approaches of contemporary piano education system from the perspective of music knowledge integration. In the process of the piano teaching, teacher and school the training of musicianship as the key point of teaching. This is because the skill can through student plays to display directly, appraises the student performance technique quality an important criterion. However, the student has the excellent adept technique merely also by far insufficient, but also needs the high artistic aesthetic ability, not only need be able to appreciate the piano performance, but must be able to reveal the artistic aesthetic feature in the process of piano performance. Piano education as one of the ways of music education, and other subjects, there is the subject of the universal education as its educational ideas, educational ideas and teaching methods, a direct impact on the generation after generation of the comprehensive quality of education. Under this basis, we propose the new idea on the issues that will be beneficial.
