Aiming at the issue of the grinding integrity of face-gear with worm wheel, the envelope mathematical model of shaper, worm wheel and face-gear is established based on theories of differential geometry and gear mesh. ...Aiming at the issue of the grinding integrity of face-gear with worm wheel, the envelope mathematical model of shaper, worm wheel and face-gear is established based on theories of differential geometry and gear mesh. The judgment of completely grinding the face-gear with the avoidance of singularities is established, and the mathematical expression to show the reason why singularities appear is derived, through the research on the surface contact area and singularity rules of the worm thread surface. The disadvantage of current face-gear grinding method that only part of the working surface of the face-gear can be covered is analyzed and the influence of coefficient of judgment is studied through changing the design parameters.展开更多
Worm grinding has been applied to manufacture gears to pursue high accuracy and fine surface finish.When the worm used to grind face gears is manufactured with multi-axis computer numerical control(CNC)machining,the m...Worm grinding has been applied to manufacture gears to pursue high accuracy and fine surface finish.When the worm used to grind face gears is manufactured with multi-axis computer numerical control(CNC)machining,the machining accuracy is usually improved by increasing the number of tool paths with more time cost.Differently,this work proposes a generated method to improve the efficiency by dressing the worm surface with only one path,and a closed-loop manufacturing process is applied to ensure the machining accuracy.According to an advanced geometric analysis,the worm surface is practically approximated as a swept surface generated by a planar curve.Meanwhile,this curve is applied as the profile of a dressing wheel,which is used to dress the worm surface.The practical machining is carried out in a CNC machine tool,which was originally used to grind helical gears.Finally,a closed-loop manufacturing process including machining,measurement,and modification is proposed to compensate the machining errors.The proposed method is validated with simulations and practical experiments.展开更多
The nitrogen source requirements for riboflavin production by ccpA mutant Bacillus subtilis 24A1/pMX45 were optimized using linear regression. The optimal medium components considered included 8% glucose as carbon sou...The nitrogen source requirements for riboflavin production by ccpA mutant Bacillus subtilis 24A1/pMX45 were optimized using linear regression. The optimal medium components considered included 8% glucose as carbon source, 2% yeast powder, 0.05% MgSO4 ·7H2O, and four types of nitrogen sources ： 0.1% yeast extract, 2% soybean powder, 1% corn plasm, and 0.2% （ NH4 ） 2 HPO4 in shake flask tests. Predictive ellipsoid was applied to determining the response values under the optimal levels for riboflavin production and glucose consumption. The optimal concentrations of the four types of nitrogen sources can remedy ammonium assimilative defection of ccpA mutant. Under the optimal conditions, the riboflavin yield increases to more than 5.0 g/L and 8%, glucose can be consumed completely after 60 h.展开更多
In this paper, we established a finite element (FEM) model to analyze the dynamic characteristics of arch bridges. In this model, the effects of adjustment to the length of a suspender on its geometry stiffness matrix...In this paper, we established a finite element (FEM) model to analyze the dynamic characteristics of arch bridges. In this model, the effects of adjustment to the length of a suspender on its geometry stiffness matrix are stressed. The FEM equations of mechanics characteristics, natural frequency and main mode are set up based on the first order matrix perturbation theory. Applicantion of the proposed model to analyze a real arch bridge proved the improvement in the simulation precision of dynamical characteristics of the arch bridge by considering the effects of suspender length variation.展开更多
Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L...Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L-1. The con centration of carbencillin (carb) was 500 mg L-1. 14 transgenic cauliflower pla nts were obtained. The putative transformants were assayed by PCR and Southern b lotting analysis. The results indicated that CpTI gene was transferred into caul iflower successfully.展开更多
We report the recovery of a 7068-nt viral sequence from the ＂viral fossils＂ embedded in the genome of Alhagi sparsifolia, a typical desert plant. Although the full viral genome remains to be completed, the putative ...We report the recovery of a 7068-nt viral sequence from the ＂viral fossils＂ embedded in the genome of Alhagi sparsifolia, a typical desert plant. Although the full viral genome remains to be completed, the putative genome structure, the deduced amino acids and phylogenetic analysis unambiguously demonstrate that this viral sequence represents a novel species of the genus Badnavirus. The putative virus is tentatively termed Alhagi bacilliform virus （ABV）. Southern blotting and inverse polymerase chain reaction （PCR） data indicate that the ABV-related sequence is integrated into the A. sparsifolia genome, and probably does not give rise to functional episomal virus. Molecular evidence that the ABV sequence exists widely in A. sparsifolia is also presented. To our knowledge, this is the first endogenous badnavirus identified from plants in the Gobi desert, and may provide new clues on the evolution, geo- graphical distribution as well as the host range of the badnaviruses.展开更多
基金Projects(51275530,51535012) supported by the National Natural Science Foundation of ChinaProject(2011CB706800) supported by the National Basic Research Program of China
文摘Aiming at the issue of the grinding integrity of face-gear with worm wheel, the envelope mathematical model of shaper, worm wheel and face-gear is established based on theories of differential geometry and gear mesh. The judgment of completely grinding the face-gear with the avoidance of singularities is established, and the mathematical expression to show the reason why singularities appear is derived, through the research on the surface contact area and singularity rules of the worm thread surface. The disadvantage of current face-gear grinding method that only part of the working surface of the face-gear can be covered is analyzed and the influence of coefficient of judgment is studied through changing the design parameters.
基金Project(2019 YFB 2004700)supported by the National Key R&D Project of ChinaProject(HTL-O-19 K 02)supported by National Key Laboratory of Science and Technology on Helicopter Transmission,Nanjing University of Aeronautics and Astronautics,China。
文摘Worm grinding has been applied to manufacture gears to pursue high accuracy and fine surface finish.When the worm used to grind face gears is manufactured with multi-axis computer numerical control(CNC)machining,the machining accuracy is usually improved by increasing the number of tool paths with more time cost.Differently,this work proposes a generated method to improve the efficiency by dressing the worm surface with only one path,and a closed-loop manufacturing process is applied to ensure the machining accuracy.According to an advanced geometric analysis,the worm surface is practically approximated as a swept surface generated by a planar curve.Meanwhile,this curve is applied as the profile of a dressing wheel,which is used to dress the worm surface.The practical machining is carried out in a CNC machine tool,which was originally used to grind helical gears.Finally,a closed-loop manufacturing process including machining,measurement,and modification is proposed to compensate the machining errors.The proposed method is validated with simulations and practical experiments.
文摘The nitrogen source requirements for riboflavin production by ccpA mutant Bacillus subtilis 24A1/pMX45 were optimized using linear regression. The optimal medium components considered included 8% glucose as carbon source, 2% yeast powder, 0.05% MgSO4 ·7H2O, and four types of nitrogen sources ： 0.1% yeast extract, 2% soybean powder, 1% corn plasm, and 0.2% （ NH4 ） 2 HPO4 in shake flask tests. Predictive ellipsoid was applied to determining the response values under the optimal levels for riboflavin production and glucose consumption. The optimal concentrations of the four types of nitrogen sources can remedy ammonium assimilative defection of ccpA mutant. Under the optimal conditions, the riboflavin yield increases to more than 5.0 g/L and 8%, glucose can be consumed completely after 60 h.
基金Supported by the Key Teacher Foundation of Chongqing University (No. 717411067)
文摘In this paper, we established a finite element (FEM) model to analyze the dynamic characteristics of arch bridges. In this model, the effects of adjustment to the length of a suspender on its geometry stiffness matrix are stressed. The FEM equations of mechanics characteristics, natural frequency and main mode are set up based on the first order matrix perturbation theory. Applicantion of the proposed model to analyze a real arch bridge proved the improvement in the simulation precision of dynamical characteristics of the arch bridge by considering the effects of suspender length variation.
文摘Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L-1. The con centration of carbencillin (carb) was 500 mg L-1. 14 transgenic cauliflower pla nts were obtained. The putative transformants were assayed by PCR and Southern b lotting analysis. The results indicated that CpTI gene was transferred into caul iflower successfully.
基金Project supported by the National Natural Science Foundation of China(Nos.31370181 and 31570146)the Fujian Natural Science Funds for Distinguished Young Scholar(No.2014J06008),China
文摘We report the recovery of a 7068-nt viral sequence from the ＂viral fossils＂ embedded in the genome of Alhagi sparsifolia, a typical desert plant. Although the full viral genome remains to be completed, the putative genome structure, the deduced amino acids and phylogenetic analysis unambiguously demonstrate that this viral sequence represents a novel species of the genus Badnavirus. The putative virus is tentatively termed Alhagi bacilliform virus （ABV）. Southern blotting and inverse polymerase chain reaction （PCR） data indicate that the ABV-related sequence is integrated into the A. sparsifolia genome, and probably does not give rise to functional episomal virus. Molecular evidence that the ABV sequence exists widely in A. sparsifolia is also presented. To our knowledge, this is the first endogenous badnavirus identified from plants in the Gobi desert, and may provide new clues on the evolution, geo- graphical distribution as well as the host range of the badnaviruses.
