[Objective] The aim was to study on the electrotransformation of Thermotoga maritima MS8.[Method] Square waves,exponential waves and high voltage shock were used for the electrotransformation of T.maritima MS8,and the...[Objective] The aim was to study on the electrotransformation of Thermotoga maritima MS8.[Method] Square waves,exponential waves and high voltage shock were used for the electrotransformation of T.maritima MS8,and the obtained transformants were detected by PCR.[Result] A single square electric pulse could be applied to cell sample in 0.2 cm ET cuvettes by using a Bio-Rad Gene Pulser set at 150 V,25 ms at room temperature,but the transformation efficiency was very low.[Conclusion] This research may improve the transformation efficiency of T.maritima MS8.展开更多
To improve the operational efficiency of global optimization in engineering, Kriging model was established to simplify the mathematical model for calculations. Ducted coaxial-rotors aircraft was taken as an example an...To improve the operational efficiency of global optimization in engineering, Kriging model was established to simplify the mathematical model for calculations. Ducted coaxial-rotors aircraft was taken as an example and Fluent software was applied to the virtual prototype simulations. Through simulation sample points, the total lift of the ducted coaxial-rotors aircraft was obtained. The Kriging model was then constructed, and the function was fitted. Improved particle swarm optimization(PSO) was also utilized for the global optimization of the Kriging model of the ducted coaxial-rotors aircraft for the determination of optimized global coordinates. Finally, the optimized results were simulated by Fluent. The results show that the Kriging model and the improved PSO algorithm significantly improve the lift performance of ducted coaxial-rotors aircraft and computer operational efficiency.展开更多
基金Supported by Sub-Project of"973"(2004CB719600)National Natural Science Foundation Projects(30170511)~~
文摘[Objective] The aim was to study on the electrotransformation of Thermotoga maritima MS8.[Method] Square waves,exponential waves and high voltage shock were used for the electrotransformation of T.maritima MS8,and the obtained transformants were detected by PCR.[Result] A single square electric pulse could be applied to cell sample in 0.2 cm ET cuvettes by using a Bio-Rad Gene Pulser set at 150 V,25 ms at room temperature,but the transformation efficiency was very low.[Conclusion] This research may improve the transformation efficiency of T.maritima MS8.
基金Project(2013AA063903)supported by High-tech Research and Development Program of China
文摘To improve the operational efficiency of global optimization in engineering, Kriging model was established to simplify the mathematical model for calculations. Ducted coaxial-rotors aircraft was taken as an example and Fluent software was applied to the virtual prototype simulations. Through simulation sample points, the total lift of the ducted coaxial-rotors aircraft was obtained. The Kriging model was then constructed, and the function was fitted. Improved particle swarm optimization(PSO) was also utilized for the global optimization of the Kriging model of the ducted coaxial-rotors aircraft for the determination of optimized global coordinates. Finally, the optimized results were simulated by Fluent. The results show that the Kriging model and the improved PSO algorithm significantly improve the lift performance of ducted coaxial-rotors aircraft and computer operational efficiency.
