二叶亭四迷及其代表作《浮云》主人公文三命运管窥

二叶亭四迷[ふたばていしめい](1864—1909年),原名长谷川辰之助[はせがわたつのすけ],笔名二叶亭四迷。近代日本著名作家、俄罗斯文学翻译家。其长篇小说《浮云》被誉为日本近代现实主义文学的开山之作,且译介到我国受到广泛关注;《浮云》实质性地描述了一个普通小职员处于命运多舛的现实境地,他甚至连基本正常的生存需求都难以维继;《浮云》折射出现实社会背景下,普通人的生存困境,同时也凸显人间世态炎凉。

《永乐大典戏文三种》中“将”字句的处置式与致使式考察

以《戏文三种》为语料,对“将”字句的用法进行穷尽式地考察,可以发现,“将”字的主流用法是用作介词。从汉语史的角度,简要探讨了“将”字由动词发展为介词的语法化过程。并着重从语义、句式两个角度,对“将”字的介词用法进行了分析。

Studies on the Isolation, Identification and In Vitro Growth Rates of the Three Pathogenic Fungi from Panax notoginseng Cultivated in Wenshan Eparchy

Objective] The aim of this study was to simultaneously isolate and identify the main pathogenic fungi of the root rot, black spot and round spot from the Panax notoginseng plants cultivated in Wenshan Eparchy of Yunnan Province of China. [Method] The pathogenic fungi were isolated and purified by using potato dextrose agar （PDA） medium. The morphological identification was accomplished first according to the colony forms of the fungi when cultivated in vitro, then accord-ing to the symptom characteristics and colony forms of the re-isolated fungi in the reverse inoculation experiments. The molecular identification was performed accord-ing to the amplification and alignment of the internal transcribed space （ITS） se-quences of the fungi. The increases of the diameters and thickness of the colonies of the fungi cultivated in vitro were employed to indicate the growth rates of the fungi. [Results] The consistency of the colony forms and symptom characteristics and the 96%-99% similarities revealed in the ITS sequence alignments al proved that the main pathogenic fungi of the root rot, black spot and round spot of the P. notoginseng plants raised in Wenshan were Cylindrocarpon didymium, Alternaria panax and Mycocentrospora acerina, respectively. When cultivated in vitro in the same temperature, humidity and il umination, the increases of the colony diameters and thickness of C. didymium were the highest, fol owed by those of A. panax, then those of M. acerina. During different cultivation periods, the differences of the colony diameters and thickness of the three fungi al reached extremely significant level. However, at the same cultivation time, the differences of the diameters and thickness among the three fungi only reached significant level. [Conclusion] The main pathogenic fungi which result in the root rot, black spot and round spot of the P. notoginseng in Wenshan are C. didymium, A. panax and M. acerina, respec-tively. When these three diseases break out at the same time, the root rot wil spread fastest, fol owed orderly by the black spot and the round spot.

Forest Evapotranspiration in Pearl River Delta based on Remote Sensing Technology

Evapotranspiration ratio of six major forest types in the Pearl River Delta was studied in this paper, and the six forest types included eucalyptus forest, fruit forest, coniferous forest, shrub forest, coniferous forest, and broad-leaved mixed for- est. The mechanism of PT-T model was analyzed based on Priestley-Taylor ex- tended model, and the result showed that land surface temperature or temperature difference between day and night could be used to evaluate the evapotranspiration factor. The evapotranspiration of 6 forest types was estimated with MODIS data and TM data and weather information during 2008-2009. The result suggested that water consumption of different forest differs insignificantly, and the EF of eucalyptus is not high.

GRAPH GRAMMAR METHOD FOR 3-D CORNER DETECTION

Most of local feature descriptors assume that the scene is planar. In the real scene, the captured images come from the 3-D world. 3-D corner as a novel invariant feature is important for the image matching and the object detection, while automatically discriminating 3-D corners from ordinary corners is difficult. A novel method for 3-D corner detection is proposed based on the image graph grammar, and it can detect the 3-D features of corners to some extent. Experimental results show that the method is valid and the 3-D corner is useful for image matching.

Correlation of eco-hydrographic benefit and height increment of Robinia pseudoacacia stand with climatic environmental factors in Yellow River Delta Wetland of China

The relationship between eco-hydrographic benefit of forest vegetation and climatic environmental factors is one of the focuses in the research on environmental protection and ecosystem countermeasures in Wetland. The runoff, sediment and soil moisture rate dynamics in Robinia pseudoacacia plantation and its clearcut area were investigated in the natural runoff experiment plots in Yellow River Delta Wet- land, Shandong Province, China. The correlation of height increment ofR. pseudoacacia with nine climate factors such as light, water, heat, etc. was analyzed by stepwise regression analysis. The results showed that the amounts of runoff and sediment in clearcut area of R. pseudoacacia were 53.9%-150.8% and 172.8%-387.1% higher than that in Robinia pseudoacacia plantation, respectively. The runoff peak value in R. pseudoacacia stand was obviously lower than that in clerarcut area, meantime, the occurrence of runoffpeak in R. pseudoacacia stand was 25 min later than in its clerarcut area. The soil moisture rates in R. pseudoacacia stand and its clearcut varied periodically with annual rainfall precipitation in both dry season and humid season. The annual mean soil moisture rate in R. pseudoacacia stand was 23.3%-25.6% higher than that in its clearcut area. Meanwhile, a regression model reflecting the correlation between the height increment of R. pseudoacacia and climatic factors was developed by stepwise regression procedure method. It showed that the light was the most important factor for the height increment ofR. pseudoacacia, followed by water and heat factors.

郑玄注《三礼》方法略探

自郑玄注解《三礼》之后,始有《三礼》之名。郑玄注经,先是统观全局,提纲挈领,总体上纲举目张,以点带面,从而达到"解一卷而众篇明"的目的。并根据已有的研究成果,兼采今、古,既注重名物训诂,又注重章句之释,吸纳今文经学派和古文经学派互不兼容的注经方式。然后再具体而微,深入到经文的具体字词,兼用直训和义界的方法注解之。如此,从面到点,从全局到具体,郑玄注经犹如层层剥笋,既兼顾得当,有条不紊,又透彻深入,巨细无遗。

《王三》重文中的非异体现象刍议

宋濂跋本王仁啕《刊谬补缺切韵》（以下简称《王三》）收录了1600余组重文，这些重文是以记词功能相同为标准而录入《王三》的，并非全是异体字。从构形和功能两个层面对《王三》重文材料进行定性分析，发现除异体字外，还包含假借字、同源字、古今字、同义替换字等，这些重文与异体字有着本质区别。厘清《王三》中的这些非异体现象，有助于更全面深入地认识《王三》重文。

Tupu methods of spatial-temporal pattern on land use change:a case study in the Yellow River Delta

Comprehensive study on land-use change of spatial pattern and temporal process is the key component in LUCC study nowadays. Based on the theories and methods of Geo-information Tupu (Carto-methodology in Geo-information, CMGI), integration of spatial pattern and temporal processes of land-use change in the Yellow River Delta (YRD) are studied in the paper, which is supported by ERDAS and ARC/INFO software. The main contents include: (1) concept models of Tupu by spatial-temporal integration on land-use change, whose Tupu unit is synthesized by "Spatial·Attribute·Process" features and composed of relatively homogeneous geographical unit and temporal unit; (2) data sources and handling process, where four stages of spatial features in 1956, 1984, 1991, and 1996 are acquired; (3) integration of series of temporal-spatial Tupu, reconstruction series of "Arising" Tupu, spatial-temporal Process Tupu and the spatial temporal Pattern Tupu on land-use change by remap tables; (4) Pattern Tupu analysis on land-use change in YRD during 1956-1996; and (5) spatial difference of the Pattern Tupu analysis by dynamic Tupu units. The various landform units and seven sub-deltas generated by the Yellow River since 1855 are different. The Tupu analysis on land-use in the paper is a promising try on the comprehensive research of "spatial pattern of dynamic process" and "temporal process of spatial pattern" in LUCC research. The Tupu methodology would be a powerful and efficient tool on integrated studies of spatial pattern and temporal process in Geo-science.

Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor

To investigate a suitable long-term culture system and optimal cryopreservation of intestinal organoid to improve organoid-based therapy by acquiring large numbers of cells.METHODSCrypts were isolated from jejunum of C57BL/6 mouse. Two hundred crypts were cultured in organoid medium with either epidermal growth factor/Noggin/R-spondin1 (ENR) or ENR/CHIR99021/VPA (ENR-CV). For subculture, organoids cultured on day 7 were passaged using enzyme-free cell dissociation buffer (STEMCELL Technologies). The passage was performed once per week until indicated passage. For cryopreservation, undissociated and dissociated organoids were resuspended in freezing medium with or without Rho kinase inhibitor subjected to different treatment times. The characteristics of intestinal organoids upon extended passage and freeze-thaw were analyzed using EdU staining, methyl thiazolyl tetrazolium assay, qPCR and time-lapse live cell imaging.RESULTSWe established a three-dimensional culture system for murine small intestinal organoids using ENR and ENR-CV media. Both conditions yielded organoids with a crypt-villus architecture exhibiting Lgr5+ cells and differentiated intestinal epithelial cells as shown by morphological and biochemical analysis. However, during extended passage (more than 3 mo), a comparative analysis revealed that continuous passaging under ENR-CV conditions, but not ENR conditions induced phenotypic changes as observed by morphological transition, reduced numbers of Lgr5+ cells and inconsistent expression of markers for differentiated intestinal epithelial cell types. We also found that recovery of long-term cryopreserved organoids was significantly affected by the organoid state, i.e., whether dissociation was applied, and the timing of treatment with the Rho-kinase inhibitor Y-27632. Furthermore, the retention of typical morphological characteristics of intestinal organoids such as the crypt-villus structure from freeze-thawed cells was observed by live cell imaging.CONCLUSIONThe maintenance of the characteristics of intestinal organoids upon extended passage is mediated by ENR condition, but not ENR-CV condition. Identified long-term cryopreservation may contribute to the establishment of standardized cryopreservation protocols for intestinal organoids for use in clinical applications.

Strengthening the three-dimensional comprehensive observation system of multi-layer interaction on the Tibetan Plateau to cope with the warming and wetting trend

Changes in the water cycle on the Tibetan Plateau(TP)have a significant impact on local agricultural production and livelihoods and its downstream regions.Against the background of widely reported warming and wetting,the hydrological cycle has accelerated and the likelihood of extreme weather events and natural disasters occurring(i.e.,snowstorms,floods,landslides,mudslides,and ice avalanches)has also intensified,especially in the highelevation mountainous regions.Thus,an accurate estimation of the intensity and variation of each component of the water cycle is an urgent scientific question for the assessment of plateau environmental changes.Following the transformation and movement of water between the atmosphere,biosphere and hydrosphere,the authors highlight the urgent need to strengthen the three-dimensional comprehensive observation system(including the eddy covariance system;planetary boundary layer tower;profile measurements of temperature,humidity,and wind by microwave radiometers,wind profiler,and radiosonde system;and cloud and precipitation radars)in the TP region and propose a practical implementation plan.The construction of such a three-dimensional observation system is expected to promote the study of environmental changes and natural hazards prevention.

Discourse Markers of “Saying”:A Comparative Study

The present study compares the discourse markers(DMs) of “saying” in Mandarin Chinese ke yi shuo(lit.“can say”) and ying gai shuo(lit.“should say”) with their equivalents in English,by drawing the parallel corpora from The Selected Works of Deng Xiaoping(Volume III)(henceforth The Works),so as to uncover the pragmatic motivation for the variation in translating the DMs of“saying.” The analysis is conducted within a theoretical framework of speech act theory,by grouping the DMs according to their personal pronouns.Moreover,by comparing the uses of personal pronouns in English and Chinese,the current study confirms that The Works keeps the consistency between the two languages as well as with the characteristics and styles of Deng Xiaoping’s locution.It also finds that modalized DMs of “saying”(e.g.,“I should say”),as a parenthesis,lose their status as matrix clauses and help reduce the tone of affirmation.

Geomorphic Habitat Type, Drift Cell, Forage Fish and Juvenile Salmon： Are They Linked？

The role of geomorphic habitat type, drift cell scale, and geographic scale in defining fish use of nearshore habitats is poorly known, particularly for Pacific salmon and their prey. In this study, key areas of nearshore habitat in central and western Strait of Juan de Fuca were categorized by geomorphic habitat type and assessed for fish use within a degraded （Elwha） and intact comparative drift cells over a one year period. Juvenile Chinook and coho salmon were also sampled for genetic analysis to define regional dispersal patterns. Key findings are： （1） Ecological function of the area＇s nearshore is complex, with very strong seasonal variation in fish use both within and across GMHT （geomorphic habitat type）; （2） GMHT link to nearshore function for fish use differs depending on the fish species and time of year. Surf smelt and sand lance were the most abundant. And they were seasonally used embayed, spit, and bluff shorelines more than lower rivers. Juvenile Chinook, coho, and chum salmon occurred in much lower density than forage fish species, and used lower rivers more than other GMHTs; （3） When GMHTs were combined and analyzed at the drift cell scale, the degraded drift cell had different ecological patterns than the intact drift cell; （4） Cross regional juvenile fish use of nearshore is an important component of habitat use： juvenile Chinook and coho from as far away as the Columbia River Oregon and Klamath River California utilize central Strait of Juan de Fuca shorelines. Forage fish species may do so as well. Drift cell and cross regional scales are therefore most important for accurately defining nearshore ecological function, management, and restoration actions.

Giant retroperitoneal malignant schwannoma:A case report and review of literature

The retroperitoneal malignant schwannoma is an extremely rare pathological entity and sparsely reported in the literature.We reported a case of a 56-year-old man who was admitted to our hospital for giant retroperitoneal malignant schwannoma with 40 cm in diameter.The case did not accompanied by neurofibromatosis.We used spiral CT 3D reconstruction to identify the characters of the tumor and the invasion to the peripheral organs before surgery.The patient has been survived for more than 8 years after radical surgery.Its clinical presentation, radiological, histopathological features, and radical surgery were retrospective analysis.

Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease

AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds.

Spatial Variation of Dissolved Organic Carbon in Soils of Riparian Wetlands and Responses to Hydro-geomorphologic Changes in Sanjiang Plain, China

Spatial variation of dissolved organic carbon(DOC) in soils of riparian wetlands and responses to hydro-geomorphologic changes in the Sanjiang Plain were analyzed through in situ collecting soil samples in the Naoli River and the Bielahong River. The results showed that the average contents of DOC for soil layer of 0–100 cm were 730.6 mg/kg, 250.9 mg/kg, 423.0 mg/kg and 333.1 mg/kg respectively from riverbed to river terrace along the transverse directions of the Naoli watershed. The content of the soil DOC was the highest in the riverbed, lower in the high floodplain and much lower in the river terrace, and it was the lowest in the low floodplain. The difference in the content and vertical distribution of DOC between the riverbed and the three riparian wetlands was significant, while it was not significant among the low floodplain, the high floodplain and the river terrace. The variability of soil DOC was related to the hydrological connectivity between different landscape position of the riparian wetlands and the adjacent stream. Extremely significant correlations were observed between DOC and total organic carbon(TOC), total iron(TFe), ferrous iron(Fe(II)) whose correlation coefficients were 0.819, –0.544 and –0.709 in riparian wetlands of the Naoli River. With the increase of wetland destruction, soil p H increased and soil DOC content changed. The correlation coefficients between soil DOC and TOC, TFe, Fe(II) also changed into 0.759, –0.686 and –0.575 respectively in the Bielahong River. Under the impact of drainage ditches, the correlations between soil DOC and TFe, Fe(II) were not obvious, while the soil p H was weakly alkaline and was negatively correlated with soil DOC in the previous high floodplain. It indicates that riparian hydro-geomorphology is the main factor that could well explain this spatial variability of soil DOC, and the agricultural environmental hydraulic works like ditching also must be considered.

Methodology for Definition of the Three-Dimensional Geodesic Structures in the Olinda＇s Historical Site

It is shown to be a relevant study involving terrestrial methods of measurement, such as： forward and backward intersections, geometric leveling, trigonometric leveling with short distance targeted and spatial positioning GNSS methods, for the definition of field reference points and field-object points located in rough terrain. The geodesic structures were implemented in the Historic Site of Olinda employing GNSS （global navigation satellite system） receivers, total stations and digital level. The historical site of Olinda was recorded by UNESCO as Historical and Cultural Heritage of Humanity. The study area is located in the center of the busiest site with a quite roughly relief. This area has been studied since 2007 involving Research of Scientific Initiation and Pos-Graduation Course. This paper aims to present the realized experiments for the implementation and definition of geodesic structures in environments with very rough relief, including large old houses and historic monuments.

Infinitesimal Methods in the Works of Thabit ibn Qurra and L, Euler in Spherical Trigonometry

In one of his astronomical works the prominent arabic medieval scientists Thabit ibn Qurra （836-901） studied the visible motion of the Sun and found the points, where its velocity is maximum or minimum. He also lbund the points on the ecliptic, where this velocity is equal to the average velocity of the Sun over all the ecliptic. For this purpose he used the idea of infinitely small arcs and their ratios in different points of the circle. The great scientist Leonard Euler （1707-1783） introduced in his works on spherical trigonometry the line-element ds of the surface of the sphere, i.e. the differential of the arc length. He constructed the spherical trigonometry as an inner geometry on the surface of the sphere. He replaced the trigonometry lines, which were in use befbre him, by trigonometric functions.

Discretization Approach in Integrated Hydrologic Model for Surface and Groundwater Interaction

The commonly used discretization approaches for distributed hydrological models can be broadly categorized into four types,based on the nature of the discrete components:Regular Mesh,Triangular Irregular Networks(TINs),Representative Elementary Watershed(REWs) and Hydrologic Response Units(HRUs).In this paper,a new discretization approach for landforms that have similar hydrologic properties is developed and discussed here for the Integrated Hydrologic Model(IHM),a combining simulation of surface and groundwater processes,accounting for the interaction between the systems.The approach used in the IHM is to disaggregate basin parameters into discrete landforms that have similar hydrologic properties.These landforms may be impervious areas,related areas,areas with high or low clay or organic fractions,areas with significantly different depths-to-water-table,and areas with different types of land cover or different land uses.Incorporating discrete landforms within basins allows significant distributed parameter analysis,but requires an efficient computational structure.The IHM integration represents a new approach interpreting fluxes across the model interface and storages near the interface for transfer to the appropriate model component,accounting for the disparate discretization while rigidly maintaining mass conservation.The discretization approaches employed in IHM will provide some ideas and insights which are helpful to those researchers who have been working on the integrated models for surface-groundwater interaction.

CLOBAL CHARM OF THE CHANGJIANG RIVER DELTA

Based on the theory of geo-economy,under the new situation of global economy,information network and China’s entry into WTO,also with the holding of APEC (in 2001) and the International Exposition in the near future,the Changjiang (Yangtze) River Delta is striding toward the spectacular international multi-polar situation and becomes one of core regions with high-speed development. Facing the ocean and world all along,leading the progressive tides of the age and scintillating the splendor of the nation,she does advance with time. Through a long period of irrigation projects construction and intensive operation of lands in previous agricultural society,the artificial wetland ecosystem with a positive cycle had ever been formed in this region. At present,environmental pollution and urban expansion resulted from post-industrialization are being rectified. The delta will be the paradigm of industrial and agricultural modernization along the sustainable development road. With the rapid development of urbanization,she has been one of the regions with the highest density population and high urbanization level. Taking the Changjiang River estuary and the Hangzhou Bay as two parts,she is continuously strengthening and adjusting her interior structure,expanding mothball space and constructing the oriental modern "logistics center" to link the whole world. The butterfly-style urban system of the Changjiang River Delta is flying,probably engendering earthshaking "butterfly effect".