Traditional air conditioner used to be in local control mode. However, with the development of modern information technology, the control of air conditioner will have to support many more functions. As a first step ...Traditional air conditioner used to be in local control mode. However, with the development of modern information technology, the control of air conditioner will have to support many more functions. As a first step in such a development, we have implemented a remote monitoring system of unitary air conditioners, using the manufacturing message specification (MMS). MMS is an OSI application protocol designed for the remote control and monitoring of real devices. The functions supported are those that an operator would do normally at the console of the unitary air conditioner. Due to the accomplishment of UMCS (Unitary air conditioner MMS Control System), there will be a leap of the application of MMS and the control technology of unitary air conditioner.展开更多
[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important fac...[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important factors affecting PCR reaction system for random single-stranded DNA pool including Mg2＋ concentration, dNTP concentration, amount of Taq DNA polymerase, primer concentration and amount of random single-stranded DNA pool at four levels. Meanwhile, the annealing temperature and number of PCR reaction cycles were optimized to establish the optimal reaction system and PCR procedure. [Result] The optimal combination of PCR reaction system for random ssDNA pool was obtained, with a total system volume of 20 μl containing 2.0 μl of 10 × Buffer, 0.5 ng of random ssDNA pool, 2.5 mmol/L Mg2＋, 0.25 mmol/L dNTP Mixture, 0.6 μmol/L upstream and downstream primers and 1.5 U of Taq DNA polymerase; the optimal annealing temperature was 68 ℃ and the optimal number of cycles was 12. Under the above conditions, clear and stable bands with high specificity for random ssDNA pool were amplified. [Conclusion] This study laid the foundation for selection of parameters with higher specificity in SELEX technology.展开更多
文摘Traditional air conditioner used to be in local control mode. However, with the development of modern information technology, the control of air conditioner will have to support many more functions. As a first step in such a development, we have implemented a remote monitoring system of unitary air conditioners, using the manufacturing message specification (MMS). MMS is an OSI application protocol designed for the remote control and monitoring of real devices. The functions supported are those that an operator would do normally at the console of the unitary air conditioner. Due to the accomplishment of UMCS (Unitary air conditioner MMS Control System), there will be a leap of the application of MMS and the control technology of unitary air conditioner.
基金Supported by Central University Basic Research Operating Expenses Special Fund(XDJK2011C026)Southwest University Doctoral Fund(09BSR04)~~
文摘[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important factors affecting PCR reaction system for random single-stranded DNA pool including Mg2＋ concentration, dNTP concentration, amount of Taq DNA polymerase, primer concentration and amount of random single-stranded DNA pool at four levels. Meanwhile, the annealing temperature and number of PCR reaction cycles were optimized to establish the optimal reaction system and PCR procedure. [Result] The optimal combination of PCR reaction system for random ssDNA pool was obtained, with a total system volume of 20 μl containing 2.0 μl of 10 × Buffer, 0.5 ng of random ssDNA pool, 2.5 mmol/L Mg2＋, 0.25 mmol/L dNTP Mixture, 0.6 μmol/L upstream and downstream primers and 1.5 U of Taq DNA polymerase; the optimal annealing temperature was 68 ℃ and the optimal number of cycles was 12. Under the above conditions, clear and stable bands with high specificity for random ssDNA pool were amplified. [Conclusion] This study laid the foundation for selection of parameters with higher specificity in SELEX technology.
