In the study, analysis was made on present situation and development measures of geographical indications and cultural heritage protection of famous teas in Hubei Province. In addition, 8 related suggestions were prop...In the study, analysis was made on present situation and development measures of geographical indications and cultural heritage protection of famous teas in Hubei Province. In addition, 8 related suggestions were proposed as well.展开更多
With the accelerating development of modern residential districts and the improvement of living levels, the requirement of people on environment is increasingly enhanced as well as the demand for culture. Based on peo...With the accelerating development of modern residential districts and the improvement of living levels, the requirement of people on environment is increasingly enhanced as well as the demand for culture. Based on people-oriented principle, this design utilized Chinese 'Fu' (blessings) culture to express people’s eager for a better life and the atmosphere of good fortune. It also produced the image of ecological environment by designing water landscapes and plant landscapes, created active space like squares for activity and active water system and developed the characteristics of residential districts by designing landscape sketches and plant landscapes.展开更多
Huizhou-style gardens have been greatly influenced by Huizhou culture, their plant landscapes have also been integrated with various aspects of Huizhou culture, which demonstrated the ideology, historical and cultural...Huizhou-style gardens have been greatly influenced by Huizhou culture, their plant landscapes have also been integrated with various aspects of Huizhou culture, which demonstrated the ideology, historical and cultural connotation of the society in an abstract and impalpable way. In this study, Huizhou-style miniature landscapes, plants of virtual implications, flowers or fruit trees of special connotations are taken for examples to analyze the influence of Xin’an Neo-Confucianism on plant landscapes in Huizhou-style gardens, the demonstration of Huizhou businessmen’s aesthetic tastes, as well as folk cultures of Huizhou, which implies that plant landscapes in Huizhou-style gardens are those incorporating with humanistic spirits, ornamental and practical uses. This study aims to provide references for further researches in the present situation of ancient Huizhou areas, protection of ancient Huizhou-style gardens and construction of new-style Huizhou gardens.展开更多
A sequential extraction method for the fractionation of phosphorus (P) in lake sediments was used to analyze phos- phorus fractions of sediments taken from three large, shallow. eutrophic freshwater lakes of China-T...A sequential extraction method for the fractionation of phosphorus (P) in lake sediments was used to analyze phos- phorus fractions of sediments taken from three large, shallow. eutrophic freshwater lakes of China-Talhu Lake. Chaohu Lake, and Long.an Lake. All three lakes are located in the lower reaches of the Changjiang River (Yangtze River). In Taihu Lake and Chaohu Lake, algae blooms occurred every year, while Longgan Lake was a macrophyte-dominated lake. Results showed that exchangeable phosphorus fractions were much higher in the eutrophic lake sediments than in the macrophyte-flourishing lake sediment. Also, the ratio of Fe:P in the sediments of the algae-predomlnant lakes was generally much lower than that in the macrophyte-predominant lakes. Thus, the geochemical fractions of phosphorus in sediments had a closer relationship with the type of aquatic vegetation.展开更多
To expand knowledge on microbial communities of various metal-rich levels of mine drainage environments in Anhui province, China, the archaeal and bacterial diversities were examined using a PCR-based cloning approach...To expand knowledge on microbial communities of various metal-rich levels of mine drainage environments in Anhui province, China, the archaeal and bacterial diversities were examined using a PCR-based cloning approach. Eight acid mine water samples were collected from five areas in Tongling. Phylogenetic analyses revealed that bacteria mainly fell into ten divisions, which were Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi. Archaea fell into three phylogenetic divisions, Thermoplasma, Ferroplasma and Thermogymnomonas. The unweighted pair group method with arithmetic mean(UPGMA) cluster analysis based on the microbial communities’ compositions revealed that five samples shared similarity with the dominance of Meiothermus and Thermomonas. Two samples had the preponderant existence of Acidithiobacillus and Leptospirillum. The remaining sample owned higher microbial communities’ diversity with the Shannon-Weaver H up to 2.91. Canonical correlation analysis(CCA) suggested that microbial community structures had great association with p H and the concentration of Hg2+, Pb2+, Fe3+, Cl-, SO2- 4in water.展开更多
The grain size and palinology of sediment and the frequency of ^14C dada provide an integrated reconstruction of the Holocene warm-humid phases of the North China Plain. Two clear intense and long-lasting warm-humid p...The grain size and palinology of sediment and the frequency of ^14C dada provide an integrated reconstruction of the Holocene warm-humid phases of the North China Plain. Two clear intense and long-lasting warm-humid phases were identified by comprehensive research in this region. The first phase was dated back to the early Holocene (9 000-7 000 a BP), and the second was centered at 5 000-3 000 a BP. The warm-humid episode between 9 000 and 7 000 a BP was also recognized at other sites showing global climatic trends rather than local events. Compared with the concern to the warm-humid phase of the early Holocene, the second one was not paid enough attention in the last few decades. The compilation of the Holocene paleoclimate data suggests that perhaps the second warm-humid phase was pervasive in monsoon region of China. In perspective of environmental archaeology, much attention should be devoted to it, because the flourish and adaptation of the Neolithic cultures and the building up of the first state seem to corresponding to the general warm-humid climatic conditions of this period. In addition, a warm-humid interval at 7 20045 500 a BP was recognized by the grain size data from three sites. However, this warm-humid event was not shown in pollen assemblage and temporal distribution of ^14C data. Perhaps, the resolution for climatic reconstruction from pollen and temporal distribution of ^14C data cited here is relatively low and small-amplitude and short-period climatic events cannot be well reflected by the data. Due to the difference in locality and elevation of sampling site, as well as in resolution of proxy records, it is difficult to make precise correlation. Further work is needed in the future.展开更多
The secondary metabolites synthesized by plants are economically important chemical compounds in the agricultural and industrial areas such as food, perfumery and pharmaceutical sectors. In recent years, attempts for ...The secondary metabolites synthesized by plants are economically important chemical compounds in the agricultural and industrial areas such as food, perfumery and pharmaceutical sectors. In recent years, attempts for their production by in vitro plant cell and tissue cultures have been accelerated considerably. Colchicine, the principle secondary metabolite of Colchicum autumnale L. and Gloriosa superba L., is an important alkaloid that has poison effect used for treatment of various diseases and plant breeding studies. Presently, colchicine has been produced by using the seeds of C. autumnale L. and the tubers of G. superba L. through different chemical extraction methods. Applying in vitro plant cell and tissue cultures together with metabolic and genetic engineering techniques, large-scale production of colchicine can be achieved from the above two plant species.展开更多
Nitrogen (4, 10, 20 and 40g/m2) and phosphorus (1.2, 4.8 and 9.6g/m2) were applied to tanks to evaluating the effects of N and P additions on plant tissue nutrition and the biomass of two freshwater wetland plants in ...Nitrogen (4, 10, 20 and 40g/m2) and phosphorus (1.2, 4.8 and 9.6g/m2) were applied to tanks to evaluating the effects of N and P additions on plant tissue nutrition and the biomass of two freshwater wetland plants in the Sanjiang Plain of Northeast China, namely Carex lasiocarpa and Carex meyeriana. For C. lasiocarpa, the total N concentration (TN) of plant tissues under the treatment of 10g/m2 was lower compared with the other N treatments. Initially, C. lasiocarpa exhibited a significant increase of biomass as compared with the control value, reaching the maximum of 31.20±4.01g/tank under the treatment of 10g/m2, and then dropped to 18.02±1.53g/tank under the treatment of 40g/m2. For C. meyeriana, TN generally increased with increasing amount of N applied. High N applied produced more aboveground biomass than low N applied. C. meyeriana, as the accompanying species, can adapt itself to the wetland enriched by N, and it may replace C. lasiocarpa as the dominant species of wetland. The total P concentration (TP) in tissues of C. lasiocarpa increased with P addition. The aboveground biomass of C. lasiocarpa increased with P addition, and it changed from 18.77±3.29g/tank to 46.03±3.95g/tank. However, TP of tissue may accelerate the C. meyeriana development under the treatment of 1.2g/m2. P accumulation contributes to the dominance of C. lasiocarpa but limits the production of C. meyeriana, and the latter may disappear gradually from the wetland enriched by P. Increased input of N and P might have an influence on wetland plant community composition and structure, so the effects of nutrient inputs and accumulation should be considered to protect the freshwater wetland.展开更多
The effect of four bottom substrates, oyster shell powder(OP), sugarcane bagasse(SB), a mixture of OP and SB(OS) and fresh soil(FS), on the water quality and bacterial and zooplankton density of intensive shrimp(Litop...The effect of four bottom substrates, oyster shell powder(OP), sugarcane bagasse(SB), a mixture of OP and SB(OS) and fresh soil(FS), on the water quality and bacterial and zooplankton density of intensive shrimp(Litopenaeus vannamei) culture tanks without water change and the growth performance of cultured shrimp were compared in this study. At the end of a 110 days culturing trial, the total ammonium-N(TAN) of the water on SB and the nitrite nitrogen(NO2-N) on OS was significantly lower than that on the other substrates(P<0.05), which coincided with the high density of ammonium- and nitrite-oxidizing bacteria in the water on SB and OS, respectively. The concentration of chlorophyll a(Chl a) increased slowly on OP, SB and OS but remained low on FS. The density of total bacteria on OP, SB and OS was one order of magnitude higher than that on FS, and the density of zooplankton on SB and OS was significantly higher than that on FS or OP(P<0.05). The improved water quality and increased density of bacteria and zooplankton on SB and OS may have had a synergistic effect on shrimp culture, improving its growth performance(high survival rate and yield and low feed conversion rate). SB and OS were more effective for improving the growth performance of intensively cultured L. vannamei without water change than OP and FS. To our knowledge, this study presents the first evidence regarding the effect of different bottom substrates on intensive shrimp culture.展开更多
Distribution patterns of plant species are believed to be impacted by small-scale habitat heterogeneity. However, there have been few comparative studies examining how woody vegetation composition and diversity varies...Distribution patterns of plant species are believed to be impacted by small-scale habitat heterogeneity. However, there have been few comparative studies examining how woody vegetation composition and diversity varies with aspects of different orientations in the Trans-Himalayan region at a local scale. Here, we examined the effects of incoming solar radiation on variation in woody species composition and compared the diversity between the northeast- and southwest-facing slopes in a Trans-Himalayan valley of Nepal. We also examined the implicit interactions between slope orientation and land use in determining the compositional variations between the slopes. We selected two pairs of northeast- and southwest-facing slopes where the first pair has a similar land use and differs in exposure only(Pisang site) while the other pair has clear differences in land use in addition to slope exposure(Braka site). In each site, we sampled 72 plots(36 on each slope) in which the presence and absence of woody species, environmental variables, and disturbance were recorded. Correspondence Analysis(CA) results suggested that the woody species composition significantly varied between northeast- and southwest-facing slopes at both sites, and was significantly correlated with measured environmental variables such as radiation index, altitude, and canopy openness. In the Braka site,mean alpha diversity was significantly higher on southwest-facing slopes. In contrast, beta diversity and gamma diversity were greater on northeast-facing slopes at both sites. Our results suggest that topographic variables(e.g., radiation index) affect species composition between the slopes, likely due to their influence on small scale abiotic environmental variables. However, the effects of land use, such as livestock browsing/grazing may interact with the effects of slope exposure, effectively reducing differences in species composition within slopes but enhancing the differences in beta diversity between contrasting slopes in the Braka. We conclude that slope orientation and land use are important factors in structuring the woody species composition and diversity in the arid Trans-Himalayan region. We suggest that both environmental and land use variables should be taken into consideration in future studies on plant community structure along the cultural landscapes.展开更多
High enzymatic activity is required for laccase applications.Central composite design (CCD)-based response surface methodology (RSM) can effectively increase the enzymatic activity of Pleurotus ostreatus P40 in li...High enzymatic activity is required for laccase applications.Central composite design (CCD)-based response surface methodology (RSM) can effectively increase the enzymatic activity of Pleurotus ostreatus P40 in liquid substrate fermentation.Initial screening of the nutritional components was performed using a Plackett-Burman design.The variables,namely,bran,bagasse,Tween 80,and yeast extract,were found to have statistically significant effects on laccase activity.These variables were further optimized using CCD-based RSM.Optimal concentrations for the maximum laccase activity were 8.144 2 g/L bran,50 g/L bagasse,0.424 1 mL/L Tween 80,and 2.832 5 g/L yeast extract.Under optimized conditions,the maximum measured laccase activity reached 96 480 U/L,which was close to the predicted value (104 830 U/L) by RSM.Therefore,RSM can be used to optimize culture components for laccase activity from Pieurotus ostreatus P40.展开更多
Two biological nutrient removal (BNR) wastewater treatment plants (WWTPs) in Thailand were selected for study: the Nonghkaem WWTP and the Suvarnabhumi Airport WWTP. For each site the influents, effluents, and sup...Two biological nutrient removal (BNR) wastewater treatment plants (WWTPs) in Thailand were selected for study: the Nonghkaem WWTP and the Suvarnabhumi Airport WWTP. For each site the influents, effluents, and supernatant liquids from anaerobic sludge digesters were analyzed for total Kjeldahl nitrogen (TKN), total nitrogen (TN), total chemical oxygen demand (TCOD), biodegradable chemical oxygen demand (bCOD), and biochemical oxygen demand (BOD). Nitrogen removal efficiencies in the Nonghkaem WWTP and the Suvarnabhumi Airport WWTP were evaluated. Inadequate nitrogen removal at the Nonghkaem centralized WWTP was found during the summer period. Influent ratios of bCOD:N at the Nonghkaem plant and the Suvarnabhumi Airport plant were 2.42:1-5.45:1 and 4.1:1-6.5:1, respectively. The efficacy of addition of molasses as a carbon source for enriched denitrifying culture in a BNR process at Nonghkaem was studied. Fluorescent in situ hybridization technique (FISH) was used to identify specific nitrifying bacteria (Nitrosomonas spp., Nitrobacter spp. and Nitrospira spp.). Nitrospira spp. was the most prevalent species in the aeration tank at the Nonghkaem WWTP. This result from FISH suggests that there were significantly low oxygen and nitrite concentration in the aeration tank at the Nonghkaem WWTP during a period of low nitrogen removal.展开更多
Peculiarities of the tungsten deposits drainage flow chemical composition formation, the development of which was ceased almost 40 years ago, have been considered. Migration peculiarities of ore components have been c...Peculiarities of the tungsten deposits drainage flow chemical composition formation, the development of which was ceased almost 40 years ago, have been considered. Migration peculiarities of ore components have been covered, and forms of their migration have been calculated. Inertial characteristics of the surface flow contamination are shown.展开更多
Seasonal variations in the biochemical composition and the reproductive cycle of pen shell Atrina pectinata in Bohai Sea were investigated from May 2013 to April 2014. Histological analysis indicated that the reproduc...Seasonal variations in the biochemical composition and the reproductive cycle of pen shell Atrina pectinata in Bohai Sea were investigated from May 2013 to April 2014. Histological analysis indicated that the reproductive cycle of A. pectinata can be divided into two phases, inactive stage and gametogenesis, which were equally and significantly influenced by seawater temperature and food availability. Gametogenesis began in late autumn(October), and completed in June and July. Spawning took place in August, coinciding with the highest water temperature and the richest phytoplankton. The significantly high glycogen content in adductor muscles sustained throughout the late active and ripeness stages, but plummeted during spawning and inactive stages. The protein content in female gonads exhibited a synchronous increase along with oocyte diameter and lipid content, suggesting that the female gonads could accumulate protein and lipid for vitelline in A. pectinata. Furthermore, the RNA/DNA ratio was found to be a useful index to indicate the level of gonad maturation in both males and females. The findings of the present study provided a foundation for the fishery resource administration and the aquaculture development of this species.展开更多
Human beings do not live in nature, they live in culture. Contrary to biological evolution, which is based on genetic inheritance, cultural change is transmitted by instruction. Simple beginnings of culture have been ...Human beings do not live in nature, they live in culture. Contrary to biological evolution, which is based on genetic inheritance, cultural change is transmitted by instruction. Simple beginnings of culture have been observed in various animals. However, the much advanced human culture became possible by its transmission by language, and by its being cumulative. The concept of cultural change as a progressive process, popular by 18th and 19th centuries thinkers, is nowadays rejected, and the uniqueness of each culture stressed. Although, there are some similarities between biological evolution and cultural change, important differences between these processes are emphasized. The rapid rate of cultural change (especially its technological aspect), as compared to the rate of biological evolution, is noted. In addition, some cultural changes led to new phenomena, which in a time of crisis can be annulled. The possible consequences of the different rate of these two processes for humans as individuals, and for their societies are discussed.展开更多
Hydrocarbon contamination may affect the soil microbial community, in terms of both diversity and function. A laboratory experiment was set-up, with a semi-arid control soil and the same soil but artificially contamin...Hydrocarbon contamination may affect the soil microbial community, in terms of both diversity and function. A laboratory experiment was set-up, with a semi-arid control soil and the same soil but artificially contaminated with diesel oil, to follow changes in the dominant species of the microbial community in the hydrocarbon-polluted soil via proteomics. Analysis of the proteins extracted from enriched cultures growing in Luria-Bertani (LB) media showed a change in the microbial community. The majority of the proteins were related to gIycolysis pathways, structural or protein synthesis. The results showed a relative increase in the complexity of the soil microbial community with hydrocarbon contamination, especially after 15 days of incubation. Species such as Ralstonia solanacearum, Synechococcus elongatus and different Clostridium sp. were adapted to contamination, not appearing in the control soil, although Bacillus sp. dominated the growing in LB in any of the treatments. We conclude that the identification of microbial species in soil extracts by culture-dependent proteomics is able to partially explain the changes in the diversity of the soil microbial community in hydrocarbon polluted semi-arid soils, but this information is much more limited than that provided by molecular methods.展开更多
基金Supported by Project of Scientific and Technological Innovations by Ministry of Culture(2011021)Foundation Project of Humanities and Social Sciences of Education Department (11YJA850019)Project supported by "11th Five-Year Plan" of Hubei Socia Sciences Foundation ([2010]274)~~
文摘In the study, analysis was made on present situation and development measures of geographical indications and cultural heritage protection of famous teas in Hubei Province. In addition, 8 related suggestions were proposed as well.
文摘With the accelerating development of modern residential districts and the improvement of living levels, the requirement of people on environment is increasingly enhanced as well as the demand for culture. Based on people-oriented principle, this design utilized Chinese 'Fu' (blessings) culture to express people’s eager for a better life and the atmosphere of good fortune. It also produced the image of ecological environment by designing water landscapes and plant landscapes, created active space like squares for activity and active water system and developed the characteristics of residential districts by designing landscape sketches and plant landscapes.
文摘Huizhou-style gardens have been greatly influenced by Huizhou culture, their plant landscapes have also been integrated with various aspects of Huizhou culture, which demonstrated the ideology, historical and cultural connotation of the society in an abstract and impalpable way. In this study, Huizhou-style miniature landscapes, plants of virtual implications, flowers or fruit trees of special connotations are taken for examples to analyze the influence of Xin’an Neo-Confucianism on plant landscapes in Huizhou-style gardens, the demonstration of Huizhou businessmen’s aesthetic tastes, as well as folk cultures of Huizhou, which implies that plant landscapes in Huizhou-style gardens are those incorporating with humanistic spirits, ornamental and practical uses. This study aims to provide references for further researches in the present situation of ancient Huizhou areas, protection of ancient Huizhou-style gardens and construction of new-style Huizhou gardens.
基金Project supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (No.KZCX1-SW-12)
文摘A sequential extraction method for the fractionation of phosphorus (P) in lake sediments was used to analyze phos- phorus fractions of sediments taken from three large, shallow. eutrophic freshwater lakes of China-Talhu Lake. Chaohu Lake, and Long.an Lake. All three lakes are located in the lower reaches of the Changjiang River (Yangtze River). In Taihu Lake and Chaohu Lake, algae blooms occurred every year, while Longgan Lake was a macrophyte-dominated lake. Results showed that exchangeable phosphorus fractions were much higher in the eutrophic lake sediments than in the macrophyte-flourishing lake sediment. Also, the ratio of Fe:P in the sediments of the algae-predomlnant lakes was generally much lower than that in the macrophyte-predominant lakes. Thus, the geochemical fractions of phosphorus in sediments had a closer relationship with the type of aquatic vegetation.
基金Project(41171418)supported by the National Natural Science Foundation of China
文摘To expand knowledge on microbial communities of various metal-rich levels of mine drainage environments in Anhui province, China, the archaeal and bacterial diversities were examined using a PCR-based cloning approach. Eight acid mine water samples were collected from five areas in Tongling. Phylogenetic analyses revealed that bacteria mainly fell into ten divisions, which were Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi. Archaea fell into three phylogenetic divisions, Thermoplasma, Ferroplasma and Thermogymnomonas. The unweighted pair group method with arithmetic mean(UPGMA) cluster analysis based on the microbial communities’ compositions revealed that five samples shared similarity with the dominance of Meiothermus and Thermomonas. Two samples had the preponderant existence of Acidithiobacillus and Leptospirillum. The remaining sample owned higher microbial communities’ diversity with the Shannon-Weaver H up to 2.91. Canonical correlation analysis(CCA) suggested that microbial community structures had great association with p H and the concentration of Hg2+, Pb2+, Fe3+, Cl-, SO2- 4in water.
基金Supported by the Humanity and Social Sciences Research of Ministry of Education, China (No. 06JC780001)the Major Program of the National Natural Science Foundation of China (No. 40599420)
文摘The grain size and palinology of sediment and the frequency of ^14C dada provide an integrated reconstruction of the Holocene warm-humid phases of the North China Plain. Two clear intense and long-lasting warm-humid phases were identified by comprehensive research in this region. The first phase was dated back to the early Holocene (9 000-7 000 a BP), and the second was centered at 5 000-3 000 a BP. The warm-humid episode between 9 000 and 7 000 a BP was also recognized at other sites showing global climatic trends rather than local events. Compared with the concern to the warm-humid phase of the early Holocene, the second one was not paid enough attention in the last few decades. The compilation of the Holocene paleoclimate data suggests that perhaps the second warm-humid phase was pervasive in monsoon region of China. In perspective of environmental archaeology, much attention should be devoted to it, because the flourish and adaptation of the Neolithic cultures and the building up of the first state seem to corresponding to the general warm-humid climatic conditions of this period. In addition, a warm-humid interval at 7 20045 500 a BP was recognized by the grain size data from three sites. However, this warm-humid event was not shown in pollen assemblage and temporal distribution of ^14C data. Perhaps, the resolution for climatic reconstruction from pollen and temporal distribution of ^14C data cited here is relatively low and small-amplitude and short-period climatic events cannot be well reflected by the data. Due to the difference in locality and elevation of sampling site, as well as in resolution of proxy records, it is difficult to make precise correlation. Further work is needed in the future.
文摘The secondary metabolites synthesized by plants are economically important chemical compounds in the agricultural and industrial areas such as food, perfumery and pharmaceutical sectors. In recent years, attempts for their production by in vitro plant cell and tissue cultures have been accelerated considerably. Colchicine, the principle secondary metabolite of Colchicum autumnale L. and Gloriosa superba L., is an important alkaloid that has poison effect used for treatment of various diseases and plant breeding studies. Presently, colchicine has been produced by using the seeds of C. autumnale L. and the tubers of G. superba L. through different chemical extraction methods. Applying in vitro plant cell and tissue cultures together with metabolic and genetic engineering techniques, large-scale production of colchicine can be achieved from the above two plant species.
基金Under the auspices of the National Basic Research Program of China (No. 2004CB418502), the project of Chinese Academy of Sciences (No. KZCX3-SW-332)
文摘Nitrogen (4, 10, 20 and 40g/m2) and phosphorus (1.2, 4.8 and 9.6g/m2) were applied to tanks to evaluating the effects of N and P additions on plant tissue nutrition and the biomass of two freshwater wetland plants in the Sanjiang Plain of Northeast China, namely Carex lasiocarpa and Carex meyeriana. For C. lasiocarpa, the total N concentration (TN) of plant tissues under the treatment of 10g/m2 was lower compared with the other N treatments. Initially, C. lasiocarpa exhibited a significant increase of biomass as compared with the control value, reaching the maximum of 31.20±4.01g/tank under the treatment of 10g/m2, and then dropped to 18.02±1.53g/tank under the treatment of 40g/m2. For C. meyeriana, TN generally increased with increasing amount of N applied. High N applied produced more aboveground biomass than low N applied. C. meyeriana, as the accompanying species, can adapt itself to the wetland enriched by N, and it may replace C. lasiocarpa as the dominant species of wetland. The total P concentration (TP) in tissues of C. lasiocarpa increased with P addition. The aboveground biomass of C. lasiocarpa increased with P addition, and it changed from 18.77±3.29g/tank to 46.03±3.95g/tank. However, TP of tissue may accelerate the C. meyeriana development under the treatment of 1.2g/m2. P accumulation contributes to the dominance of C. lasiocarpa but limits the production of C. meyeriana, and the latter may disappear gradually from the wetland enriched by P. Increased input of N and P might have an influence on wetland plant community composition and structure, so the effects of nutrient inputs and accumulation should be considered to protect the freshwater wetland.
基金supported by National Science and Technology Supporting Program of the Twelfth Five-Year Plan of China (2011BAD13B10)the Special Fund for Agro-scientific Research in the Public Interest, China (201103034)
文摘The effect of four bottom substrates, oyster shell powder(OP), sugarcane bagasse(SB), a mixture of OP and SB(OS) and fresh soil(FS), on the water quality and bacterial and zooplankton density of intensive shrimp(Litopenaeus vannamei) culture tanks without water change and the growth performance of cultured shrimp were compared in this study. At the end of a 110 days culturing trial, the total ammonium-N(TAN) of the water on SB and the nitrite nitrogen(NO2-N) on OS was significantly lower than that on the other substrates(P<0.05), which coincided with the high density of ammonium- and nitrite-oxidizing bacteria in the water on SB and OS, respectively. The concentration of chlorophyll a(Chl a) increased slowly on OP, SB and OS but remained low on FS. The density of total bacteria on OP, SB and OS was one order of magnitude higher than that on FS, and the density of zooplankton on SB and OS was significantly higher than that on FS or OP(P<0.05). The improved water quality and increased density of bacteria and zooplankton on SB and OS may have had a synergistic effect on shrimp culture, improving its growth performance(high survival rate and yield and low feed conversion rate). SB and OS were more effective for improving the growth performance of intensively cultured L. vannamei without water change than OP and FS. To our knowledge, this study presents the first evidence regarding the effect of different bottom substrates on intensive shrimp culture.
基金Annapurna Conservation Area Project (ACAP)Norwegian State Educational Loan Fund (Lnekassen)+2 种基金Faculty of Mathematics and Natural Sciences,University of Bergen for financingfunded by Norwegian Research Council(project no.148910/730)supported by The Norwegian Council for Higher Education Programme for Development Research and Education(NUFU Project ID:PRO 04/2002)
文摘Distribution patterns of plant species are believed to be impacted by small-scale habitat heterogeneity. However, there have been few comparative studies examining how woody vegetation composition and diversity varies with aspects of different orientations in the Trans-Himalayan region at a local scale. Here, we examined the effects of incoming solar radiation on variation in woody species composition and compared the diversity between the northeast- and southwest-facing slopes in a Trans-Himalayan valley of Nepal. We also examined the implicit interactions between slope orientation and land use in determining the compositional variations between the slopes. We selected two pairs of northeast- and southwest-facing slopes where the first pair has a similar land use and differs in exposure only(Pisang site) while the other pair has clear differences in land use in addition to slope exposure(Braka site). In each site, we sampled 72 plots(36 on each slope) in which the presence and absence of woody species, environmental variables, and disturbance were recorded. Correspondence Analysis(CA) results suggested that the woody species composition significantly varied between northeast- and southwest-facing slopes at both sites, and was significantly correlated with measured environmental variables such as radiation index, altitude, and canopy openness. In the Braka site,mean alpha diversity was significantly higher on southwest-facing slopes. In contrast, beta diversity and gamma diversity were greater on northeast-facing slopes at both sites. Our results suggest that topographic variables(e.g., radiation index) affect species composition between the slopes, likely due to their influence on small scale abiotic environmental variables. However, the effects of land use, such as livestock browsing/grazing may interact with the effects of slope exposure, effectively reducing differences in species composition within slopes but enhancing the differences in beta diversity between contrasting slopes in the Braka. We conclude that slope orientation and land use are important factors in structuring the woody species composition and diversity in the arid Trans-Himalayan region. We suggest that both environmental and land use variables should be taken into consideration in future studies on plant community structure along the cultural landscapes.
基金National Science&Technology Pillar Program of China(No.2012BAC02B04)National Natural Science Foundation of China(No.41201306)
文摘High enzymatic activity is required for laccase applications.Central composite design (CCD)-based response surface methodology (RSM) can effectively increase the enzymatic activity of Pleurotus ostreatus P40 in liquid substrate fermentation.Initial screening of the nutritional components was performed using a Plackett-Burman design.The variables,namely,bran,bagasse,Tween 80,and yeast extract,were found to have statistically significant effects on laccase activity.These variables were further optimized using CCD-based RSM.Optimal concentrations for the maximum laccase activity were 8.144 2 g/L bran,50 g/L bagasse,0.424 1 mL/L Tween 80,and 2.832 5 g/L yeast extract.Under optimized conditions,the maximum measured laccase activity reached 96 480 U/L,which was close to the predicted value (104 830 U/L) by RSM.Therefore,RSM can be used to optimize culture components for laccase activity from Pieurotus ostreatus P40.
文摘Two biological nutrient removal (BNR) wastewater treatment plants (WWTPs) in Thailand were selected for study: the Nonghkaem WWTP and the Suvarnabhumi Airport WWTP. For each site the influents, effluents, and supernatant liquids from anaerobic sludge digesters were analyzed for total Kjeldahl nitrogen (TKN), total nitrogen (TN), total chemical oxygen demand (TCOD), biodegradable chemical oxygen demand (bCOD), and biochemical oxygen demand (BOD). Nitrogen removal efficiencies in the Nonghkaem WWTP and the Suvarnabhumi Airport WWTP were evaluated. Inadequate nitrogen removal at the Nonghkaem centralized WWTP was found during the summer period. Influent ratios of bCOD:N at the Nonghkaem plant and the Suvarnabhumi Airport plant were 2.42:1-5.45:1 and 4.1:1-6.5:1, respectively. The efficacy of addition of molasses as a carbon source for enriched denitrifying culture in a BNR process at Nonghkaem was studied. Fluorescent in situ hybridization technique (FISH) was used to identify specific nitrifying bacteria (Nitrosomonas spp., Nitrobacter spp. and Nitrospira spp.). Nitrospira spp. was the most prevalent species in the aeration tank at the Nonghkaem WWTP. This result from FISH suggests that there were significantly low oxygen and nitrite concentration in the aeration tank at the Nonghkaem WWTP during a period of low nitrogen removal.
基金Supported by RF Ministry of IndustryScience, grant No.1566. 2003. 05.
文摘Peculiarities of the tungsten deposits drainage flow chemical composition formation, the development of which was ceased almost 40 years ago, have been considered. Migration peculiarities of ore components have been covered, and forms of their migration have been calculated. Inertial characteristics of the surface flow contamination are shown.
基金supported by grants from the Scientific and Technical Supporting Program (No. 2011BAD13B01)the National Marine Public Welfare Research Program (No. 201305005)Doctoral Program of Ministry of Education of China (No. 20130132110009)
文摘Seasonal variations in the biochemical composition and the reproductive cycle of pen shell Atrina pectinata in Bohai Sea were investigated from May 2013 to April 2014. Histological analysis indicated that the reproductive cycle of A. pectinata can be divided into two phases, inactive stage and gametogenesis, which were equally and significantly influenced by seawater temperature and food availability. Gametogenesis began in late autumn(October), and completed in June and July. Spawning took place in August, coinciding with the highest water temperature and the richest phytoplankton. The significantly high glycogen content in adductor muscles sustained throughout the late active and ripeness stages, but plummeted during spawning and inactive stages. The protein content in female gonads exhibited a synchronous increase along with oocyte diameter and lipid content, suggesting that the female gonads could accumulate protein and lipid for vitelline in A. pectinata. Furthermore, the RNA/DNA ratio was found to be a useful index to indicate the level of gonad maturation in both males and females. The findings of the present study provided a foundation for the fishery resource administration and the aquaculture development of this species.
文摘Human beings do not live in nature, they live in culture. Contrary to biological evolution, which is based on genetic inheritance, cultural change is transmitted by instruction. Simple beginnings of culture have been observed in various animals. However, the much advanced human culture became possible by its transmission by language, and by its being cumulative. The concept of cultural change as a progressive process, popular by 18th and 19th centuries thinkers, is nowadays rejected, and the uniqueness of each culture stressed. Although, there are some similarities between biological evolution and cultural change, important differences between these processes are emphasized. The rapid rate of cultural change (especially its technological aspect), as compared to the rate of biological evolution, is noted. In addition, some cultural changes led to new phenomena, which in a time of crisis can be annulled. The possible consequences of the different rate of these two processes for humans as individuals, and for their societies are discussed.
基金Supported by the JAE-Program for Ph.D. Students of Spanish Research Council
文摘Hydrocarbon contamination may affect the soil microbial community, in terms of both diversity and function. A laboratory experiment was set-up, with a semi-arid control soil and the same soil but artificially contaminated with diesel oil, to follow changes in the dominant species of the microbial community in the hydrocarbon-polluted soil via proteomics. Analysis of the proteins extracted from enriched cultures growing in Luria-Bertani (LB) media showed a change in the microbial community. The majority of the proteins were related to gIycolysis pathways, structural or protein synthesis. The results showed a relative increase in the complexity of the soil microbial community with hydrocarbon contamination, especially after 15 days of incubation. Species such as Ralstonia solanacearum, Synechococcus elongatus and different Clostridium sp. were adapted to contamination, not appearing in the control soil, although Bacillus sp. dominated the growing in LB in any of the treatments. We conclude that the identification of microbial species in soil extracts by culture-dependent proteomics is able to partially explain the changes in the diversity of the soil microbial community in hydrocarbon polluted semi-arid soils, but this information is much more limited than that provided by molecular methods.