抗体(antibody)又称免疫球蛋白(immunoglobulin,Ig),是人体免疫反应的重要参与者.了解抗体的结构和结构动态特征,是理解人体免疫作用机理、修复或提高免疫能力、定向设计抗体以治疗各种疾病的基础.本文以人体IgG1抗体为对象,综述了使用...抗体(antibody)又称免疫球蛋白(immunoglobulin,Ig),是人体免疫反应的重要参与者.了解抗体的结构和结构动态特征,是理解人体免疫作用机理、修复或提高免疫能力、定向设计抗体以治疗各种疾病的基础.本文以人体IgG1抗体为对象,综述了使用透射电子显微学方法研究IgG1抗体结构方向的最新进展.详细介绍了使用逐个分子的电子断层三维重构技术(individual-particle electron tomography,IPET)对抗体进行结构研究的方法,包括样品制备、图像处理和数据分析等.并描述了利用该技术,在研究抗体结合肽分子后的结构形变和通过收集不同构象来研究抗体动态结构特征方面所取得的阶段性成果.最后,对尚待解决的关键问题与该技术未来的发展方向进行了讨论与展望.展开更多
The construction of complex stratigraphic surfaces is widely employed in many fields, such as petroleum exploration, geological modeling, and geological structure analysis. It also serves as an important foundation fo...The construction of complex stratigraphic surfaces is widely employed in many fields, such as petroleum exploration, geological modeling, and geological structure analysis. It also serves as an important foundation for data visualization and visual analysis in these fields. The existing surface construction methods have several deficiencies and face various difficulties, such as the presence of multitype faults and roughness of resulting surfaces. In this paper, a surface modeling method that uses geometric partial differential equations （PDEs） is introduced for the construction of stratigraphic surfaces. It effectively solves the problem of surface roughness caused by the irregularity of stratigraphic data distribution. To cope with the presence of multitype complex faults, a two-way projection algorithm between three- dimensional space and a two-dimensional plane is proposed. Using this algorithm, a unified method based on geometric PDEs is developed for dealing with multitype faults. Moreover, the corresponding geometric PDE is derived, and an algorithm based on an evolutionary solution is developed. The algorithm proposed for constructing spatial surfaces with real data verifies its computational efficiency and its ability to handle irregular data distribution. In particular, it can reconstruct faulty surfaces, especially those with overthrust faults.展开更多
There are many thrust-related structures occurring in the Paleozoic strata of the Niushou Mountain in the central part of Ningxia Hui Autonomous Region. The fault-related folds are the typical structures in this area....There are many thrust-related structures occurring in the Paleozoic strata of the Niushou Mountain in the central part of Ningxia Hui Autonomous Region. The fault-related folds are the typical structures in this area. Based on the analysis about these structures and their relationships, the processes by which these structures of the Miboshan Formation were formed are reconstructed, and the strata underwent about three stages of deformation: (1) horizontal shortening, (2) folding, and (3) thrusting. And the fact that the Niushou Mountain is the leading edge of an old thrust sheet was proved, the Niushou Mountain,the Daluo Mountain and the Xiaoluo Mountain together constitute the front part of this old thrust zone,so the Niushou Mountain and the Ordovician strata in the central and southern parts of Ningxia now are likely allochthons. In the period from middle Ordovician to Devonian, the areas of the central and southern Ningxia belonged to the back-arc foreland basin of North Qilianshan orogen, which was adjacent to the continent in the north. In the later part of the early Paleozoic period, the Niushou Mountain was formed after the closure of the back-arc foreland basin.展开更多
文摘抗体(antibody)又称免疫球蛋白(immunoglobulin,Ig),是人体免疫反应的重要参与者.了解抗体的结构和结构动态特征,是理解人体免疫作用机理、修复或提高免疫能力、定向设计抗体以治疗各种疾病的基础.本文以人体IgG1抗体为对象,综述了使用透射电子显微学方法研究IgG1抗体结构方向的最新进展.详细介绍了使用逐个分子的电子断层三维重构技术(individual-particle electron tomography,IPET)对抗体进行结构研究的方法,包括样品制备、图像处理和数据分析等.并描述了利用该技术,在研究抗体结合肽分子后的结构形变和通过收集不同构象来研究抗体动态结构特征方面所取得的阶段性成果.最后,对尚待解决的关键问题与该技术未来的发展方向进行了讨论与展望.
基金financially supported by the National Natural Science foundation of China(No.U1562218)
文摘The construction of complex stratigraphic surfaces is widely employed in many fields, such as petroleum exploration, geological modeling, and geological structure analysis. It also serves as an important foundation for data visualization and visual analysis in these fields. The existing surface construction methods have several deficiencies and face various difficulties, such as the presence of multitype faults and roughness of resulting surfaces. In this paper, a surface modeling method that uses geometric partial differential equations （PDEs） is introduced for the construction of stratigraphic surfaces. It effectively solves the problem of surface roughness caused by the irregularity of stratigraphic data distribution. To cope with the presence of multitype complex faults, a two-way projection algorithm between three- dimensional space and a two-dimensional plane is proposed. Using this algorithm, a unified method based on geometric PDEs is developed for dealing with multitype faults. Moreover, the corresponding geometric PDE is derived, and an algorithm based on an evolutionary solution is developed. The algorithm proposed for constructing spatial surfaces with real data verifies its computational efficiency and its ability to handle irregular data distribution. In particular, it can reconstruct faulty surfaces, especially those with overthrust faults.
基金supported by National Science Foundation(Grant No.4983402).
文摘There are many thrust-related structures occurring in the Paleozoic strata of the Niushou Mountain in the central part of Ningxia Hui Autonomous Region. The fault-related folds are the typical structures in this area. Based on the analysis about these structures and their relationships, the processes by which these structures of the Miboshan Formation were formed are reconstructed, and the strata underwent about three stages of deformation: (1) horizontal shortening, (2) folding, and (3) thrusting. And the fact that the Niushou Mountain is the leading edge of an old thrust sheet was proved, the Niushou Mountain,the Daluo Mountain and the Xiaoluo Mountain together constitute the front part of this old thrust zone,so the Niushou Mountain and the Ordovician strata in the central and southern parts of Ningxia now are likely allochthons. In the period from middle Ordovician to Devonian, the areas of the central and southern Ningxia belonged to the back-arc foreland basin of North Qilianshan orogen, which was adjacent to the continent in the north. In the later part of the early Paleozoic period, the Niushou Mountain was formed after the closure of the back-arc foreland basin.
