With the help of GIS tool of ARC/INFO, ARCVIEW and FRAGSTATS, the map of forest resource distribution of Heilongjiang Province was analyzed in 1896, 1949 and 1981. Using total area, mean patch size, patch density, coe...With the help of GIS tool of ARC/INFO, ARCVIEW and FRAGSTATS, the map of forest resource distribution of Heilongjiang Province was analyzed in 1896, 1949 and 1981. Using total area, mean patch size, patch density, coefficient of patch size variation, mean patch fractal dimension and mean shape index, we studied the change of forest landscape pattern and the change of each patch types in this region. As a result, the total area of forest landscape and mean patch size decreased sharply, the quantity and density of patches increased, the juxtaposition of patches weakened, the shape of patch tended to become regular, and the border of patch simplified. All these showed that the forest landscape of this area tended to fragment gradually, and the fragment of Korean pine forest is the severest. The diversity of whole forest landscape and the evenness of landscape types distribution reduced gradually. Human impact, instead of climate change and forest community succession, is the most important reason for such dramatic changes.展开更多
The independent influence of microstructural features on fracture toughness of TC21alloy with lamellar microstructure was investigated.Triple heat treatments were designed to obtain lamellar microstructures with diffe...The independent influence of microstructural features on fracture toughness of TC21alloy with lamellar microstructure was investigated.Triple heat treatments were designed to obtain lamellar microstructures with different parameters,which were characterized by OM and SEM.The size and content ofαplates were mainly determined by cooling rate from singleβphase field and solution temperature in two-phase field;while the precipitation behavior of secondaryαplatelets was dominantly controlled by aging temperature in two-phase field.The content and thickness ofαplates and the thickness of secondaryαplatelets were important microstructural features influencing the fracture toughness.Both increasing the content ofαplates and thickeningαplates(or secondaryαplatelets)could enhance the fracture toughness of TC21alloy.Based on energy consumption by the plastic zone of crack tip inαplates,a toughening mechanism for titanium alloys was proposed.展开更多
Pulsed field gel electrophoresis(PFGE) has been firstly introduced in characterization of the pathogenic fungi Penicillium marne f fei and Exophiala dermatitidis genomes.The numbers and sizes of their chromosomes have...Pulsed field gel electrophoresis(PFGE) has been firstly introduced in characterization of the pathogenic fungi Penicillium marne f fei and Exophiala dermatitidis genomes.The numbers and sizes of their chromosomes have been detected.Polymorphism was identified on the smallest chromosome of E.dermatitidis.The result shows that PFGE for characterization of large molecular DNA pathogenic fungi is very suitable,it is more simple and more efficacy.The result also shows the diversity of pathogenic fungi is relative common even in rare occurred pathogenic fungi such as E.dermatitidis.展开更多
AIM: To evaluate the value of CT-guided core-needle biopsy in diagnosis and classification of malignant lymphomas.METHODS: From January 1999 to October 2004, CT-guided core-needle biopsies were performed in 80 patient...AIM: To evaluate the value of CT-guided core-needle biopsy in diagnosis and classification of malignant lymphomas.METHODS: From January 1999 to October 2004, CT-guided core-needle biopsies were performed in 80 patients with suspected malignant lymphoma. Biopsies were performed with an 18-20 G biopsy-cut (CR Bard, Inc., Covington, GA,USA) needle driven by a spring-loaded Bard biopsy gun.RESULTS: A definite diagnosis and accurate histological subtype were obtained in 61 patients with a success rate of 76.25% (61/80). Surgical sampling was performed in 19 patients (23.75%) with non-diagnostic core-needle biopsies. The success rate of CT-guided core-needle biopsy varied with the histopathologic subtypes in our group.The relatively high success rates of core-needle biopsy were noted in diffuse large B-cell non-Hodgkin's lymphoma (NHL, 88.89%) and peripheral T-cell NHL (90%). However,the success rates were relatively low in anaplastic large cell (T/null cell) lymphoma (ALCL, 44.44%) and Hodgkin's disease (HD, 28.57%) in our group.CONCLUSION: CT-guided core-needle biopsy is a reliable means of diagnosing and classifying malignant lymphomas,and can be widely applied in the management of patients with suspected malignant lymphoma.展开更多
This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among ge-nomic DNA. cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells. DNA fragment...This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among ge-nomic DNA. cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells. DNA fragments encoding BR/HLH/LZ structure of Myc and Myn proteins were cloned in frame into pGEX-2T vec-tor respectively Fusion GST-Myc and GST-Myn synthe-sized in E.coli hosts showed affinity to CACGTG E-boxDNA and subsequently interacted with genomic fragments prepared through whole-genome-PCR. A PCR-assisted procedure which combines protein-DNA interaction and affinity chromatography was designed to enrich Myc/Myn bound DNA. At least two genomic DNA fragments ob- tained exhibit specifical binding capacity to Myc/Myn complex but not to GST alone. Significance of the work and of the technique itself as well as identification of the DNAs are discussed.展开更多
Objective To identify Mycobacterium marinum （M. marinum ） inducing misdiagnosis and treatment failure. Methods The lesional specimen of patient with cutaneous M. marinum were cultivated on L6wenstein-Jensen medium. ...Objective To identify Mycobacterium marinum （M. marinum ） inducing misdiagnosis and treatment failure. Methods The lesional specimen of patient with cutaneous M. marinum were cultivated on L6wenstein-Jensen medium. The isolate was identified by biochemical tests and polymerase chain reaction （PCR）-restriction fragment length polymorphism （RFLP） analysis of the hsp65 gene. Results Smooth and non- pigmented colonies were noted after incubation at 32 ℃ for 2 weeks. The isolate was acid-fast bacilli and confirmed as M. marinum by biochemical tests and PCR-RFLP. Conclusion For a correct diagnosis of cutaneous M. marinum infection, it is crucial for clinicians to have a high index of suspicion, obtain the history of exposure and trauma and understand growth characteristics of the organism. Compared with conventional biochemical techniques, PCR-RFLP analysis is a more rapid, accurate and reliable method for mycobacterial identification to species level.展开更多
Let G be a fc-regular connected vertex transitive graph. If G is not maximal restricted edge connected, then G has a (k- 1)-factor with components isomorphic to the same vertex transitive graph of order between k and ...Let G be a fc-regular connected vertex transitive graph. If G is not maximal restricted edge connected, then G has a (k- 1)-factor with components isomorphic to the same vertex transitive graph of order between k and 2k-3. This observation strenghen to some extent the corresponding result obtained by Watkins, which said that fc-regular vertex transitive graph G has a factor with components isomorphic to a vertex transitive graphs if G is not k connected.展开更多
Selecting differentially expressed genes(DEGs) is one of the most important tasks in microarray applications for studying multi-factor diseases including cancers.However,the small samples typically used in current mic...Selecting differentially expressed genes(DEGs) is one of the most important tasks in microarray applications for studying multi-factor diseases including cancers.However,the small samples typically used in current microarray studies may only partially reflect the widely altered gene expressions in complex diseases,which would introduce low reproducibility of gene lists selected by statistical methods.Here,by analyzing seven cancer datasets,we showed that,in each cancer,a wide range of functional modules have altered gene expressions and thus have high disease classification abilities.The results also showed that seven modules are shared across diverse cancers,suggesting hints about the common mechanisms of cancers.Therefore,instead of relying on a few individual genes whose selection is hardly reproducible in current microarray experiments,we may use functional modules as functional signatures to study core mechanisms of cancers and build robust diagnostic classifiers.展开更多
基金The research is supported by Study on the interaction of global change and terrestrial ecosystem in eastern China - 39899370 and the Northeast Forestry University research fund.
文摘With the help of GIS tool of ARC/INFO, ARCVIEW and FRAGSTATS, the map of forest resource distribution of Heilongjiang Province was analyzed in 1896, 1949 and 1981. Using total area, mean patch size, patch density, coefficient of patch size variation, mean patch fractal dimension and mean shape index, we studied the change of forest landscape pattern and the change of each patch types in this region. As a result, the total area of forest landscape and mean patch size decreased sharply, the quantity and density of patches increased, the juxtaposition of patches weakened, the shape of patch tended to become regular, and the border of patch simplified. All these showed that the forest landscape of this area tended to fragment gradually, and the fragment of Korean pine forest is the severest. The diversity of whole forest landscape and the evenness of landscape types distribution reduced gradually. Human impact, instead of climate change and forest community succession, is the most important reason for such dramatic changes.
文摘The independent influence of microstructural features on fracture toughness of TC21alloy with lamellar microstructure was investigated.Triple heat treatments were designed to obtain lamellar microstructures with different parameters,which were characterized by OM and SEM.The size and content ofαplates were mainly determined by cooling rate from singleβphase field and solution temperature in two-phase field;while the precipitation behavior of secondaryαplatelets was dominantly controlled by aging temperature in two-phase field.The content and thickness ofαplates and the thickness of secondaryαplatelets were important microstructural features influencing the fracture toughness.Both increasing the content ofαplates and thickeningαplates(or secondaryαplatelets)could enhance the fracture toughness of TC21alloy.Based on energy consumption by the plastic zone of crack tip inαplates,a toughening mechanism for titanium alloys was proposed.
文摘Pulsed field gel electrophoresis(PFGE) has been firstly introduced in characterization of the pathogenic fungi Penicillium marne f fei and Exophiala dermatitidis genomes.The numbers and sizes of their chromosomes have been detected.Polymorphism was identified on the smallest chromosome of E.dermatitidis.The result shows that PFGE for characterization of large molecular DNA pathogenic fungi is very suitable,it is more simple and more efficacy.The result also shows the diversity of pathogenic fungi is relative common even in rare occurred pathogenic fungi such as E.dermatitidis.
文摘AIM: To evaluate the value of CT-guided core-needle biopsy in diagnosis and classification of malignant lymphomas.METHODS: From January 1999 to October 2004, CT-guided core-needle biopsies were performed in 80 patients with suspected malignant lymphoma. Biopsies were performed with an 18-20 G biopsy-cut (CR Bard, Inc., Covington, GA,USA) needle driven by a spring-loaded Bard biopsy gun.RESULTS: A definite diagnosis and accurate histological subtype were obtained in 61 patients with a success rate of 76.25% (61/80). Surgical sampling was performed in 19 patients (23.75%) with non-diagnostic core-needle biopsies. The success rate of CT-guided core-needle biopsy varied with the histopathologic subtypes in our group.The relatively high success rates of core-needle biopsy were noted in diffuse large B-cell non-Hodgkin's lymphoma (NHL, 88.89%) and peripheral T-cell NHL (90%). However,the success rates were relatively low in anaplastic large cell (T/null cell) lymphoma (ALCL, 44.44%) and Hodgkin's disease (HD, 28.57%) in our group.CONCLUSION: CT-guided core-needle biopsy is a reliable means of diagnosing and classifying malignant lymphomas,and can be widely applied in the management of patients with suspected malignant lymphoma.
文摘This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among ge-nomic DNA. cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells. DNA fragments encoding BR/HLH/LZ structure of Myc and Myn proteins were cloned in frame into pGEX-2T vec-tor respectively Fusion GST-Myc and GST-Myn synthe-sized in E.coli hosts showed affinity to CACGTG E-boxDNA and subsequently interacted with genomic fragments prepared through whole-genome-PCR. A PCR-assisted procedure which combines protein-DNA interaction and affinity chromatography was designed to enrich Myc/Myn bound DNA. At least two genomic DNA fragments ob- tained exhibit specifical binding capacity to Myc/Myn complex but not to GST alone. Significance of the work and of the technique itself as well as identification of the DNAs are discussed.
文摘Objective To identify Mycobacterium marinum （M. marinum ） inducing misdiagnosis and treatment failure. Methods The lesional specimen of patient with cutaneous M. marinum were cultivated on L6wenstein-Jensen medium. The isolate was identified by biochemical tests and polymerase chain reaction （PCR）-restriction fragment length polymorphism （RFLP） analysis of the hsp65 gene. Results Smooth and non- pigmented colonies were noted after incubation at 32 ℃ for 2 weeks. The isolate was acid-fast bacilli and confirmed as M. marinum by biochemical tests and PCR-RFLP. Conclusion For a correct diagnosis of cutaneous M. marinum infection, it is crucial for clinicians to have a high index of suspicion, obtain the history of exposure and trauma and understand growth characteristics of the organism. Compared with conventional biochemical techniques, PCR-RFLP analysis is a more rapid, accurate and reliable method for mycobacterial identification to species level.
基金Supported by NNSF of China(10271105) Doctoral Foundation of Zhangzhou Normal College.
文摘Let G be a fc-regular connected vertex transitive graph. If G is not maximal restricted edge connected, then G has a (k- 1)-factor with components isomorphic to the same vertex transitive graph of order between k and 2k-3. This observation strenghen to some extent the corresponding result obtained by Watkins, which said that fc-regular vertex transitive graph G has a factor with components isomorphic to a vertex transitive graphs if G is not k connected.
基金supported by the National Natural Science Foundation of China (Grant Nos. 30170515,30370388 and 30970668)
文摘Selecting differentially expressed genes(DEGs) is one of the most important tasks in microarray applications for studying multi-factor diseases including cancers.However,the small samples typically used in current microarray studies may only partially reflect the widely altered gene expressions in complex diseases,which would introduce low reproducibility of gene lists selected by statistical methods.Here,by analyzing seven cancer datasets,we showed that,in each cancer,a wide range of functional modules have altered gene expressions and thus have high disease classification abilities.The results also showed that seven modules are shared across diverse cancers,suggesting hints about the common mechanisms of cancers.Therefore,instead of relying on a few individual genes whose selection is hardly reproducible in current microarray experiments,we may use functional modules as functional signatures to study core mechanisms of cancers and build robust diagnostic classifiers.
