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牛血液中一株新型环状病毒的分离与全基因组序列分析 被引量:16
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作者 杨恒 李占鸿 +5 位作者 张怡轩 高林 谢佳芮 廖德芳 吴健敏 李华春 《病毒学报》 CAS CSCD 北大核心 2018年第1期75-84,共10页
我们从广西壮族自治区哨兵动物牛上采集的血液样本中分离到一株病毒,暂将其命名为广西环状病毒(毒株号:V172/GX/2015)。病毒接种C6/36细胞后可产生明显的细胞病变,表现为细胞聚集、皱缩与脱落。高分辨率琼脂糖凝胶电泳显示,病毒基因... 我们从广西壮族自治区哨兵动物牛上采集的血液样本中分离到一株病毒,暂将其命名为广西环状病毒(毒株号:V172/GX/2015)。病毒接种C6/36细胞后可产生明显的细胞病变,表现为细胞聚集、皱缩与脱落。高分辨率琼脂糖凝胶电泳显示,病毒基因组由10节段的双链RNA组成,在凝胶上呈现"3-4-3"的带型特征。通过全长cDNA扩增与高通量测序方法获取V172/GX/2015毒株的全基因组序列。病毒基因组大小为19 889bp,基因节段的大小在3 996bp(Seg-1)至829bp(Seg-10)之间,可编码VP1至VP7等7种结构蛋白以及NS1至NS3等3种非结构蛋白。对环状病毒属保守的VP1、VP3(T2)与VP7(T13)蛋白氨基酸序列分析与系统发育树分析显示,V172/GX/2015与蚊传播环状病毒Yunnan orbivirus、Peruvian horse sickness virus以及Mobuck virus具有较近的亲缘关系,氨基酸序列相似度在31.4%至69.9%之间;V172/GX/2015在系统发育树上形成了一个独立于其它环状病毒的进化分支。本研究首次报道了一种新型环状病毒在牛上的分离与全基因组序列,研究结果将进一步丰富我们对环状病毒属病毒的认知,为开展新型环状病毒的流行病学调查与致病性研究提供基础。 展开更多
关键词 新型环状病毒 病毒分离 全基因组测序
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A Novel Aircraft Air Conditioning System with a Sterilization Unit by Ultra-High-Temperature Air Stream 被引量:3
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作者 SUN Zhi SUN Jianhong CHEN Siyu 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI CSCD 2020年第4期646-654,共9页
An aircraft cabin is a narrow,closed-space environment.To keep the air quality in cabin healthy for passengers,especially during an epidemic such as SARS-CoV-2(or 2019-nCoV)in 2020,a novel aircraft air conditioning sy... An aircraft cabin is a narrow,closed-space environment.To keep the air quality in cabin healthy for passengers,especially during an epidemic such as SARS-CoV-2(or 2019-nCoV)in 2020,a novel aircraft air conditioning system,called the ultra-high-temperature instantaneous sterilization air conditioning system(UHTACS),is proposed.Based on the proposed system,a simulation of the UHT-ACS is analysed in various flight states.In the UHT-ACS,the mixing air temperature of return and bleed air can reach temperature up to 148.8°C,which is high enough to kill bacilli and viruses in 2一8 s.The supply air temperature of the UHT-ACS in a mixing cavity is about 12 C in cooling mode both on the ground and in the air.The supply air temperature is about 42 C in heating mode.Compared with the air conditioning systems(ACS)of traditional aircraft the supply air temperatures of the UHT-ACS in the mixing cavity are in good agreement with those of a traditional ACS with 60%fresh air and 40%return air.Furthermore the air temperature at the turbine outlet of the UHT-ACS is higher than that of a traditional ACS which will help to reduce the risk of icing at the outlet.Therefore the UHT-ACS can operate normally in various flight states. 展开更多
关键词 air conditioning system(ACS) ultra-high-temperature instantaneous sterilization ultra-hightemperature instantaneous sterilization air conditioning system(UHT-ACS) return air 2019-nCoV
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