目的新型力学加载式生物反应器相同振动频率和不同振动应变作用于体外三维支架条件下培养的雄性大鼠成骨细胞,探究OPG/RANKL表达的影响。方法雄性大鼠成骨细胞培养、传代后分组:振动频率为1.0Hz,不同振动应变(A B C三组)及对照组(无振...目的新型力学加载式生物反应器相同振动频率和不同振动应变作用于体外三维支架条件下培养的雄性大鼠成骨细胞,探究OPG/RANKL表达的影响。方法雄性大鼠成骨细胞培养、传代后分组:振动频率为1.0Hz,不同振动应变(A B C三组)及对照组(无振动应变及振动频率)(D组)4组。应用RT-PCR和Western blot检测干预后OPG和RANKL mRNA和蛋白水平的表达。结果仅振动应变2000με(B组)作用于细胞后,细胞中OPGmRNA蛋白水平表达升高,RANKLmRNA及蛋白水平则降低(均P<0.05)。其余实验组未见阳性反应。结论通过新型力学加载式生物反应器(振动应变2000με振动频率1.0Hz)的压应力作用于细胞后可以正性调节OPG/RANKL轴的表达。展开更多
A novel anaerobic reactor, jet biogas inter-loop anaerobic fluidized bed （JBILAFB）, was designed and constructed. The start-up and performance of the reactor was investigated in the Process. of .artificial glucose w...A novel anaerobic reactor, jet biogas inter-loop anaerobic fluidized bed （JBILAFB）, was designed and constructed. The start-up and performance of the reactor was investigated in the Process. of .artificial glucose wastewater treatment. With the wastewater recycle ratio of 2.5 ： 1, the recycled wastewater with biogas could mix sludge and wastewater in the JBILAFB reactor completely. The start-up of the JBILAFB reactor could be completed in less than 70 d through maintenance of hydraulic retention time （HR^I＂） and stepwise increase of feed total organic carbon （TOC） concentration. After the start-up, with the volumetric TOC loadings of 14.3 kg·m ^-3·d^-1, the TOC removal ratio, the effluent pH, and the volatile fatty acids （VFA）/alkalinity of the JBILAFB reactor were more than 80%, close to 7.0 and less than 0.4, respectively. Moreover, CH4 was produced at more than 70% of the theoretical value, The reactor exhibited high stability under the condition of high volumetric TOC loading. Sludge granules in the JBILAFB reactor were developed during the start-up and their sizes were enlarged with the stepwise increase of volumetric TOC loadings from 0.8 kg.m^-3.d ^-1 to 14.3 kg.m^-3.d^-1. Granules, an offwhite color and a similar spherical shape, were mainly comprised of global-like bacteria. These had good methanogenic activity and settleability, which were formed probably through adhesion of the bacteria. Some inorganic metal compounds such as Fe, Ca, Mg, Al, etc. were advantageous to the formation of the granules.展开更多
文摘目的新型力学加载式生物反应器相同振动频率和不同振动应变作用于体外三维支架条件下培养的雄性大鼠成骨细胞,探究OPG/RANKL表达的影响。方法雄性大鼠成骨细胞培养、传代后分组:振动频率为1.0Hz,不同振动应变(A B C三组)及对照组(无振动应变及振动频率)(D组)4组。应用RT-PCR和Western blot检测干预后OPG和RANKL mRNA和蛋白水平的表达。结果仅振动应变2000με(B组)作用于细胞后,细胞中OPGmRNA蛋白水平表达升高,RANKLmRNA及蛋白水平则降低(均P<0.05)。其余实验组未见阳性反应。结论通过新型力学加载式生物反应器(振动应变2000με振动频率1.0Hz)的压应力作用于细胞后可以正性调节OPG/RANKL轴的表达。
基金Supported by the National Natural Science Foundation of China （No.50278036）, the Natural Science Foundation of Guangdong Province （No.04105951） and the National High Technology Research and Development Program of China （No.2006AA06Z378）.
文摘A novel anaerobic reactor, jet biogas inter-loop anaerobic fluidized bed （JBILAFB）, was designed and constructed. The start-up and performance of the reactor was investigated in the Process. of .artificial glucose wastewater treatment. With the wastewater recycle ratio of 2.5 ： 1, the recycled wastewater with biogas could mix sludge and wastewater in the JBILAFB reactor completely. The start-up of the JBILAFB reactor could be completed in less than 70 d through maintenance of hydraulic retention time （HR^I＂） and stepwise increase of feed total organic carbon （TOC） concentration. After the start-up, with the volumetric TOC loadings of 14.3 kg·m ^-3·d^-1, the TOC removal ratio, the effluent pH, and the volatile fatty acids （VFA）/alkalinity of the JBILAFB reactor were more than 80%, close to 7.0 and less than 0.4, respectively. Moreover, CH4 was produced at more than 70% of the theoretical value, The reactor exhibited high stability under the condition of high volumetric TOC loading. Sludge granules in the JBILAFB reactor were developed during the start-up and their sizes were enlarged with the stepwise increase of volumetric TOC loadings from 0.8 kg.m^-3.d ^-1 to 14.3 kg.m^-3.d^-1. Granules, an offwhite color and a similar spherical shape, were mainly comprised of global-like bacteria. These had good methanogenic activity and settleability, which were formed probably through adhesion of the bacteria. Some inorganic metal compounds such as Fe, Ca, Mg, Al, etc. were advantageous to the formation of the granules.
