对近年来全国各省市出现的雏鹅新型病毒性肠炎病毒(New type gosling viralenteritis virus,NGVEV)流行病学、临床症状、病理组织结构、病原学、病毒的检测和诊断、免疫预防、临床预防和治疗等方面的研究情况进行了论述。该病是由腺病...对近年来全国各省市出现的雏鹅新型病毒性肠炎病毒(New type gosling viralenteritis virus,NGVEV)流行病学、临床症状、病理组织结构、病原学、病毒的检测和诊断、免疫预防、临床预防和治疗等方面的研究情况进行了论述。该病是由腺病毒引起的一种尚未被认识的新病毒,目前仅有NGVEV高免血清的预防和治疗效果较好,其余药物和生物制剂都尚需要进一步研究和改良。这些研究结果可为进一步深入研究和开发有效的药物提供基础资料,进而可提高我国养鹅业的经济效益。展开更多
The characteristics changes of apoptosis of Duck Embryo Fibroblasts(DEF)cells induced by New type gosling viral enteritis virus,NGVEV)were observed by means of HE staining,electron microscopy and Annexin V-FITC/PI flu...The characteristics changes of apoptosis of Duck Embryo Fibroblasts(DEF)cells induced by New type gosling viral enteritis virus,NGVEV)were observed by means of HE staining,electron microscopy and Annexin V-FITC/PI fluorescent staining.During 24-48 h post infection(pi),the difference of morphological change between infected DEF cells and the mock infected cells was invisible.At 72 h pi,the nuclear chromatin was getting condensed through HE staining;apoptotic morphological change such as abnormal shape of the nucleus,condensation of the cytoplasm and chromatin were observed under electron microscope;and the early apoptotic cells(Annexin V-FITC positive and PI negative)were detected under fluorescence microscope.At 96-120 h pi,by means of HE staining and electron microscopy,the advanced morphological change of apoptosis such as formation of different kinds of apoptotic bodies,and shrink of the DEF cells and nucleus were detected;under fluorescence microscope the different stages of the apoptotic DEF can be easily distinguished:early apoptotic cells(Annexin V-FITC postive and pi negative),advanced or late apoptotic cells(both Annexin V-FITC and PI positive),necrosis cells or dead cells(Annexin V-FITC negative and PI positive).This investigation shows that NGVEV might induce apoptosis and form characteristic apoptotic morphological changes in the DEF cells.NGVEV inducement of apoptosis may be an important mechanism of efficient dissemination of virus progeny.展开更多
文摘对近年来全国各省市出现的雏鹅新型病毒性肠炎病毒(New type gosling viralenteritis virus,NGVEV)流行病学、临床症状、病理组织结构、病原学、病毒的检测和诊断、免疫预防、临床预防和治疗等方面的研究情况进行了论述。该病是由腺病毒引起的一种尚未被认识的新病毒,目前仅有NGVEV高免血清的预防和治疗效果较好,其余药物和生物制剂都尚需要进一步研究和改良。这些研究结果可为进一步深入研究和开发有效的药物提供基础资料,进而可提高我国养鹅业的经济效益。
文摘The characteristics changes of apoptosis of Duck Embryo Fibroblasts(DEF)cells induced by New type gosling viral enteritis virus,NGVEV)were observed by means of HE staining,electron microscopy and Annexin V-FITC/PI fluorescent staining.During 24-48 h post infection(pi),the difference of morphological change between infected DEF cells and the mock infected cells was invisible.At 72 h pi,the nuclear chromatin was getting condensed through HE staining;apoptotic morphological change such as abnormal shape of the nucleus,condensation of the cytoplasm and chromatin were observed under electron microscope;and the early apoptotic cells(Annexin V-FITC positive and PI negative)were detected under fluorescence microscope.At 96-120 h pi,by means of HE staining and electron microscopy,the advanced morphological change of apoptosis such as formation of different kinds of apoptotic bodies,and shrink of the DEF cells and nucleus were detected;under fluorescence microscope the different stages of the apoptotic DEF can be easily distinguished:early apoptotic cells(Annexin V-FITC postive and pi negative),advanced or late apoptotic cells(both Annexin V-FITC and PI positive),necrosis cells or dead cells(Annexin V-FITC negative and PI positive).This investigation shows that NGVEV might induce apoptosis and form characteristic apoptotic morphological changes in the DEF cells.NGVEV inducement of apoptosis may be an important mechanism of efficient dissemination of virus progeny.
