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用新法制备K88ac基因探针 被引量:1
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作者 张林元 邓小昭 《微生物学报》 CAS CSCD 北大核心 1989年第1期72-74,共3页
由于遗传工程技术的发展,基因探针被广泛应用。探针的DNA大都来自于大肠杆菌的重组质粒,这样制备的探针易污染该菌的染色体和载体DNA,作菌落杂交时易出现假阳性。为了克服这些缺点,用枯草杆菌质粒作载体克隆K88ac基因的EcoRI小片段。以... 由于遗传工程技术的发展,基因探针被广泛应用。探针的DNA大都来自于大肠杆菌的重组质粒,这样制备的探针易污染该菌的染色体和载体DNA,作菌落杂交时易出现假阳性。为了克服这些缺点,用枯草杆菌质粒作载体克隆K88ac基因的EcoRI小片段。以枯草杆菌重组质粒作探针,与含K88ac基因的菌株杂交呈阳性,而不含K88ac基因的菌株呈阴性,且阴阳性有明显区别。 展开更多
关键词 K88ac基因 基因探针 新法制备
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Research on technology innovation capability of equipment manufacturing industry in Shanghai
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作者 XUE Xia 《International English Education Research》 2017年第4期26-28,共3页
Equipment manufacturing industry is an important symbol to measure the industrial strength, scientific and technological innovation ability and international competitiveness of a country or region. Firstly, this paper... Equipment manufacturing industry is an important symbol to measure the industrial strength, scientific and technological innovation ability and international competitiveness of a country or region. Firstly, this paper analyzes the development status of equipment manufacturing industry in Shanghai, and then uses DEA to analyze the efficiency of technology innovation capability of equipment manufacturing industry in Shanghai. The results show that the equipment manufacturing industry in Shanghai has stronger overall drawing ability, and besides, the technology innovation ability of the transportation equipment manufacturing industry is weak, other equipment manufacturing sub industries have relatively strong ability of technological innovation. This paper analyzes the shortcomings of the transportation equipment manufacturing industry and gives guidance and puts forward corresponding suggestions and measures. 展开更多
关键词 Equipment manufacturing industry Technological innovation Data envelopment analysis
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