目的探讨TLR-2(Toll like receptor-2)信号途径对新生儿免疫系统在淋巴细胞增殖和调节性T细胞(Treg)扩增方面的影响及其与成人免疫系统的差别。方法应用TLR-2的配体-肽聚糖(PPG)和有丝分裂原-植物凝血素(PHA)刺激胎儿脐带血单核淋巴细胞...目的探讨TLR-2(Toll like receptor-2)信号途径对新生儿免疫系统在淋巴细胞增殖和调节性T细胞(Treg)扩增方面的影响及其与成人免疫系统的差别。方法应用TLR-2的配体-肽聚糖(PPG)和有丝分裂原-植物凝血素(PHA)刺激胎儿脐带血单核淋巴细胞(CBMC)和成人外周血单核淋巴细胞(PBMC),然后采用3H胸腺嘧啶掺入法测淋巴细胞增殖情况及流式细胞技术检测活化T细胞,Treg细胞扩增情况。结果PPG和PHA刺激后,CBMC淋巴细胞总体明显增殖(P<0.05),但PPG的刺激不能使活化T细胞和Treg细胞比例增加(P>0.05)。PPG和PHA刺激后,PBMC淋巴细胞总体明显增殖(P<0.05),且活化T细胞和Treg细胞比例均增加(P<0.05)。此外,PPG诱导的CBMC淋巴细胞增殖强度弱于PBMC(P<0.05)。结论TLR-2信号途径可以诱导新生儿淋巴细胞增殖但活化T细胞和Treg细胞比例不增加,与成人免疫系统的TLR-2信号途径存在差别。展开更多
Study results from different geographical areas provide some circumstantial evidence that, when compared with the general population, people who later in life develop multiple sclerosis (MS) have a pattern of birth ex...Study results from different geographical areas provide some circumstantial evidence that, when compared with the general population, people who later in life develop multiple sclerosis (MS) have a pattern of birth excess numbers in spring and late summer, which may disclose an association with MS predisposing environmental agents. To identify the presence of season-related cluster of MS birth in Sardinia we have designed a case-control study in the province of Sassari, Northern Sardinia, insular Italy, an area at very-high and increasing risk for MS. Mean birth incidence rate of people with MS (810 cases) on a three-and six-months basis were compared with that of two control populations: the MS unaffected siblings (1069), sharing genetic material with patients, and a representative number of births (247,612) of the general population of the study area. We found that the birth in months peaking in spring significantly represents one risk factor for future MS development. This seasonal deviation of MS births reveals an intriguing epidemiological overlap with common environmental agents, which may open a new scenario of hypothetical explanations for environmental factors perhaps affecting the CNS at the crucial time of myelination or shaping the newborn immune system.展开更多
文摘目的探讨TLR-2(Toll like receptor-2)信号途径对新生儿免疫系统在淋巴细胞增殖和调节性T细胞(Treg)扩增方面的影响及其与成人免疫系统的差别。方法应用TLR-2的配体-肽聚糖(PPG)和有丝分裂原-植物凝血素(PHA)刺激胎儿脐带血单核淋巴细胞(CBMC)和成人外周血单核淋巴细胞(PBMC),然后采用3H胸腺嘧啶掺入法测淋巴细胞增殖情况及流式细胞技术检测活化T细胞,Treg细胞扩增情况。结果PPG和PHA刺激后,CBMC淋巴细胞总体明显增殖(P<0.05),但PPG的刺激不能使活化T细胞和Treg细胞比例增加(P>0.05)。PPG和PHA刺激后,PBMC淋巴细胞总体明显增殖(P<0.05),且活化T细胞和Treg细胞比例均增加(P<0.05)。此外,PPG诱导的CBMC淋巴细胞增殖强度弱于PBMC(P<0.05)。结论TLR-2信号途径可以诱导新生儿淋巴细胞增殖但活化T细胞和Treg细胞比例不增加,与成人免疫系统的TLR-2信号途径存在差别。
文摘Study results from different geographical areas provide some circumstantial evidence that, when compared with the general population, people who later in life develop multiple sclerosis (MS) have a pattern of birth excess numbers in spring and late summer, which may disclose an association with MS predisposing environmental agents. To identify the presence of season-related cluster of MS birth in Sardinia we have designed a case-control study in the province of Sassari, Northern Sardinia, insular Italy, an area at very-high and increasing risk for MS. Mean birth incidence rate of people with MS (810 cases) on a three-and six-months basis were compared with that of two control populations: the MS unaffected siblings (1069), sharing genetic material with patients, and a representative number of births (247,612) of the general population of the study area. We found that the birth in months peaking in spring significantly represents one risk factor for future MS development. This seasonal deviation of MS births reveals an intriguing epidemiological overlap with common environmental agents, which may open a new scenario of hypothetical explanations for environmental factors perhaps affecting the CNS at the crucial time of myelination or shaping the newborn immune system.
