Study on the Developmental Changes of Muscular GHR mRNA Expression in Sheep

[ Objective] The study aimed to explore the expression of muscular growth hormone receptor gene （GHR） in sheep at the early stage of growth and development. [Method] The GHR mRNA expression levels in longissimus dorsal muscles of male Kazak sheep and Xinjiang fine wool sheep with different ages were quantitatively analyzed by real time PCR. [ Result] Sheep GHR mRNA expression level in longissimus dorsal muscle increased firstly followed by decline, and then kept steady until the end of the experiment, with the expression peak appearing on postnatal day 30. The GHR mRNA expression level of Kazak sheep was extremely lower than that of Xingjiang fine wool sheep from 2 to 90 days old （ P 〈0.01 ）. E Conclusionl Both age and breed had great effects on the expression of muscular GHR gene in sheep.

兔、羊脑泡型包虫病模型建立方法的比较

目的 探讨泡状棘球蚴（Em）新西兰兔、新疆细绵羊脑病模型构建方法,并评价其可行性及实用性.方法 将57只兔分为3组,13只羊分为2批次,采用经颅穿刺法、颈动脉穿刺法接种不同浓度Em六勾蚴及生发层混悬液,建立模型.应用临床型3.0T磁共振扫描仪及病理方法评价模型.结果 两种模型总建模成功率为16.67％;羊、兔成模率分别为36.36％、12.24％.经颅穿刺法成模率为20.83％;进针深度小于0.5 cm成模率为5.56％,进针深度0.5 ～ 1.0 cm成模率为30.00％;接种液浓度1×107/L成模率为16.22％,2×107/L成模率为36.37％.颈动脉穿刺法建模成功0只.兔模型30 d磁共振成像（MRI）可检测出病灶;90 d两种模型MRI检出率与病理检测一致.结论 颅骨直接穿刺接种感染Em,建立新西兰兔、新疆细绵羊泡型包虫病（AE）脑病模型具有可行性;能够模拟自然疫源性AE脑病.兔模型成模时间短,经济性高,适合早期病变观察;羊模型生活期长,脑容积大,适合临床及MRI完整的评价病理过程.