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安徽产石蒜属植物三种酶同工酶的分析 被引量:15
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作者 聂刘旺 张定成 +3 位作者 张海军 张小爱 柳李旺 张克云 《生物学杂志》 CAS CSCD 2000年第3期19-22,共4页
采用聚丙烯酰胺凝胶电泳技术分离同工酶 ,首次分析了安徽产石蒜属 6种植物十一个居群的过氧化物酶、酯酶、苹果酸脱氢酶三种酶同工酶 ,比较了居群间酶谱差异 ,并用数量分类学方法 ,对酶谱资料进行了聚类分析 (UPGMA法 )。结果表明 :1、... 采用聚丙烯酰胺凝胶电泳技术分离同工酶 ,首次分析了安徽产石蒜属 6种植物十一个居群的过氧化物酶、酯酶、苹果酸脱氢酶三种酶同工酶 ,比较了居群间酶谱差异 ,并用数量分类学方法 ,对酶谱资料进行了聚类分析 (UPGMA法 )。结果表明 :1、过氧化物酶、酯酶同工酶酶谱均显示出居群间的差异 ,而苹果酸脱氢酶同工酶酶谱居群间共性很大。 2、酶谱特征显示 ,三种酶系统在居群间或居群内均有多样性 ,但种间居群的酶谱差异明显大于种内居群的差异。 3、聚类结果可将 6种植物十一个居群分为两大类群 ( 2n =1 6组和 2n =2 2组 ) ,此与细胞及形态分类学结果相吻合。 4、根据同工酶的产生与进化的关系 ,本文初步探讨了种间亲缘关系。本文从分子水平上为石蒜属的分类及系统发育研究提供了有价值的资料。 展开更多
关键词 石蒜属 同工酶 聚类分析 新缘关系 系统发育
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Analysis of Genetic Relationships in Prunus domestica L. in Xinjiang Using ISSR Markers 被引量:1
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作者 孙琪 廖康 +5 位作者 耿文娟 刘娟 曼苏尔.那斯尔 刘欢 贾杨 曹倩 《Agricultural Science & Technology》 CAS 2015年第3期447-453,503,共8页
[Objective] This paper aimed to explore the genetic relationships among different types of Prunus domestica L. in Xinjiang. [Method] The inter-simple se- quence repeat (ISSR) markers were applied to analyze the gene... [Objective] This paper aimed to explore the genetic relationships among different types of Prunus domestica L. in Xinjiang. [Method] The inter-simple se- quence repeat (ISSR) markers were applied to analyze the genetic diversity and relationships among 30 individual plants from 5 types of P. domestica germpiasm resources in Xinjiang. [Result] A total of 317 bands were amplified by 16 selected IS- SR primers. Among the amplified bands, there were 246 polymorphic bands, accounting for 77.60% of the total. The Nei's gene diversity index (H) of individuals was 0.266 6. The average Shannon's information index (I) of individuals was 0.399 1. And most of the genetic similarity coefficients ranged from 0.555 2 to 0.996 8. It is indicated that the P. domestica germplasm resources have a certain genetic diversity in Xinjiang. The cluster analysis showed that, at the genetic similarity coefficient of 0.719, the 30 individuals were divided into 3 major groups, including a group of introduced European plum varieties, a group of Tacheng Smoked Plum and Tacheng Binzi and a group of Jiashi Smoked Plum and Wild European Plum. While at the genetic similarity coefficient of 0.949, Jiashi Smoked Plum and Wild European Plum were divided into two small groups. [Conclusion] There are very close genetic relationships between Jiashi Smoked Plum and Wild European Plum, and among the introduced European plum varieties, Tacheng Smoked Plum and Tacheng Binzi. 展开更多
关键词 XINJIANG Prunus domestica L. ISSR Genetic relationship
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