Aim The enhanced effect of Bushen (Kidney-tonifying) decoction (BS) oncultured PC12 cell proliferation and its antagonistic action on neurotoxicity induced by glutamatewere investigated by serum pharmacological method...Aim The enhanced effect of Bushen (Kidney-tonifying) decoction (BS) oncultured PC12 cell proliferation and its antagonistic action on neurotoxicity induced by glutamatewere investigated by serum pharmacological method of the Chinese material medica (CMM) in vitro.Methods The effect of BS on cultured PC12 cell activity and its antagonistic action on neurotoxicityinduced by glutamate was observed by MTT method. Flow cytometry and fluorescence microscopetechniques were employed to observe the antagonistic effect of BS on early period apoptosis of PC12cells induced by glutamate. Results The serum with BS was able to enhance activity of PC12 cells andexert antagonistic effect on glutamate-induced neurotoxicity. Meanwhile, these beneficial effectsproduced by BS were found to be the strongest in 20% concentration of in serum BS. Moreover, it caninhibit apoptosis of PC12 cells induced by glutamate , which occurs in the early period. ConclusionBS may exert a potential neuroprotective effect.展开更多
The increasing scale and complexity of power systems require high performance and high reliability of power system protection.Protective relaying based on directional comparison with power line carrier or microwave ch...The increasing scale and complexity of power systems require high performance and high reliability of power system protection.Protective relaying based on directional comparison with power line carrier or microwave channels is the most suitable protection scheme for long distance EHV transmission lines and is widely used in power systems.The key element of such protection is a directional relay used to discriminate the fault direction.In order to overcome the disadvantages of conventional directional relays,the authors of this paper put forward the directional comparison carrier protection based on the artificial neural network(ANN).The protection is extensively tested using electromagnetic transient program (EMTP) under various electric power system operating and fault conditions.It is proved that the directional comparison carrier protection based on ANN,which can recognize various fault patterns of the protected transmission line(such as fault direction,fault phases etc.)correctly in any kind of operating and fault conditions and the whole process,is satisfactory for EHV transmission line protection.展开更多
From the protection of Chinese white dolphin and living environment,and considering the industrial development in coastal areas,we put forward adjustment program for Jiangmen Chinese White Dolphin Provincial Nature Re...From the protection of Chinese white dolphin and living environment,and considering the industrial development in coastal areas,we put forward adjustment program for Jiangmen Chinese White Dolphin Provincial Nature Reserve. Meanwhile we analyzed the feasibility of this program by systematically focusing the effect of adjusted functional regions on Chinese white dolphin.展开更多
To ensure the safety of consumers, prolong the shelf life of moon cake and reduce the cost of manufacturing and marketing enterprises, the optimization of preservative formula and the shelf life of cantonese style moo...To ensure the safety of consumers, prolong the shelf life of moon cake and reduce the cost of manufacturing and marketing enterprises, the optimization of preservative formula and the shelf life of cantonese style moon cake were studied. Through single and compound formula preservative test, the results showed that potassium sorbate and sodium dehydroacetate could inhibit the microorganisms in the moon cake, but the inhibitory effect of sodium dehydroacetate was stronger than that of potassium sorbate; and the combination of potassium sorbate and sodium dehydroacetate had a synergistic effect, and the best formula is ＇potassium sorbate 0.44 g/kg mixed with sodium dehydroacetate 0.38 g/kg. A batch of moon cake was prepared with the best preservative formula, then the total number of colonies, molds and coliform group in the samples were detected by random sampling at irregular intervals. The total number of molds exceeded the limit on the 187^th d, and it indicated that the shelf life of the product was 187 d. Compared with the conventional 15-day shelf life, this research greatly prolongs the sheff life of cantonese style moon cake, and cut down the cost of moon cake enterprises. It is worthy of popularization and application.展开更多
AIM: To compare the preservation of non-heart- beating donor (NHBD) livers in cold histidine-trytophan- ketoglutarate (HTK) solution and extracorporeal liver perfusion (ECLP). METHODS: Livers harvested from health pig...AIM: To compare the preservation of non-heart- beating donor (NHBD) livers in cold histidine-trytophan- ketoglutarate (HTK) solution and extracorporeal liver perfusion (ECLP). METHODS: Livers harvested from health pigs were stored for 10 h in cold HTK solution (group A, n = 4) or perfused with oxygenated autologous blood at body temperature (group B, n = 4). Both groups were then tested on the circuit for 4 h. Bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of extracorporeal livers were tested in each group. Liver tissues from each group were examined at the end of reperfusion. RESULTS: At 1, 2, 3 and 4 h after reperfusion, bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of livers in group A were statistically different from those in group B (P < 0.05 or P < 0.01). CONCLUSION: ECLP is better than HTK solution to preserve NHBD livers. ECLP can assess the graft viabilitybefore liver transplantation.展开更多
To enhance the bandwidth efficiency of the guard interval （GI） assisted wireless communication system, an attractive scheme is proposed, which combines the functions of pilots and the GI together, so that the pilot ...To enhance the bandwidth efficiency of the guard interval （GI） assisted wireless communication system, an attractive scheme is proposed, which combines the functions of pilots and the GI together, so that the pilot resource used for estimating channel state is saved. Based on the proposed different known guard intervals （DKGI）, the time-domain channel estimation can be simply applied on the receiver side. After channel estimation, the receiver can employ the cyclic convolution restoring （CCR） function to reconstruct the cyclical convolution relationship between the signal and the channel, by which the receiver can also achieve good performance through the conventional 1-tap frequency domain equalization （FDE）.展开更多
Single-strand breaks （SSBs） can occur in cells either directly, or indirectly following initiation of base excision repair （BER）. SSBs generally have blocked termini lacking the conventional 5＇-phosphate and 3＇-...Single-strand breaks （SSBs） can occur in cells either directly, or indirectly following initiation of base excision repair （BER）. SSBs generally have blocked termini lacking the conventional 5＇-phosphate and 3＇-hydroxyl groups and require further processing prior to DNA synthesis and ligation. XRCC1 is devoid of any known enzymatic activity, but it can physically interact with other proteins involved in all stages of the overlapping SSB repair and BER pathways, including those that conduct the rate-limiting end-tailoring, and in many cases can stimulate their enzymatic activities. XRCC1^-/- mouse fibroblasts are most hypersensitive to agents that produce DNA lesions repaired by monofunctional glycosylase-initiated BER and that result in formation of indirect SSBs. A requirement for the deoxyribose phosphate lyase activity of DNA polymerase β （pol β） is specific to this pathway, whereas pol β is implicated in gap-filling during repair of many types of SSBs. Elevated levels of strand breaks, and diminished repair, have been demonstrated in MMS- treated XRCC1^-/-, and to a lesser extent in pol β^-/- cell lines, compared with wild-type cells. Thus a strong correlation is observed between cellular sensitivity to MMS and the ability of cells to repair MMS-induced damage. Exposure of wild-type and polβ^-/- cells to an inhibitor of PARP activity dramatically potentiates MMS-induced cytotoxicity. XRCC1^-/- cells are also sensitized by PARP inhibition demonstrating that PARP-mediated poly（ADP-ribosyl）ation plays a role in modulation of cytotoxicity beyond recruitment of XRCC 1 to sites of DNA damage.展开更多
A class of high resolution positivity preserving Boltzmann type difference schemes for one and two dimensional Euler equations is studied. First, the relation between Boltzmann and Euler equations is analyzed. By usi...A class of high resolution positivity preserving Boltzmann type difference schemes for one and two dimensional Euler equations is studied. First, the relation between Boltzmann and Euler equations is analyzed. By using a kind of special interpolation, the high resolution Boltzmann type difference scheme is constructed. Finally, numerical tests show that the schemes are effective and useful.展开更多
Poverty reduction and environmental protection are two global tasks for sustainable development.The study perspective has changed over time,from narrowly focusing on poverty reduction to comprehensively strengthening ...Poverty reduction and environmental protection are two global tasks for sustainable development.The study perspective has changed over time,from narrowly focusing on poverty reduction to comprehensively strengthening human-welfare.We reviewed key references targeting the theoretical content and practical approach relying on poverty,environment protection and ecosystem services.We discussed the contradicting views on the relationship of poverty and environmental degradation,and then illustrated the study progress of a cutting-age topic-ecosystem services which pave a way to address poverty reduction and environmental protection together.At last,we investigated essential factors that affect the development and environmental protection.Considering the evolution of the concept of poverty,we found that the environment has occupied an increasing proportion in the cognizance of poverty.The relationship between poverty and environmental degradation is regional uniqueness.In practical aspect,projects based on the management and valuation assessment of ecosystem services draw researchers′attention all over the world.The appropriate scale,essential economic incentives,morality,law and social equality are key factors affecting individuals′decisions which directly relate to the sustainable development.展开更多
Seismic inversion is a highly ill-posed problem, due to many factors such as the limited seismic frequency bandwidth and inappropriate forward modeling. To obtain a unique solution, some smoothing constraints, e.g., t...Seismic inversion is a highly ill-posed problem, due to many factors such as the limited seismic frequency bandwidth and inappropriate forward modeling. To obtain a unique solution, some smoothing constraints, e.g., the Tikhonov regularization are usually applied. The Tikhonov method can maintain a global smooth solution, but cause a fuzzy structure edge. In this paper we use Huber-Markov random-field edge protection method in the procedure of inverting three parameters, P-velocity, S-velocity and density. The method can avoid blurring the structure edge and resist noise. For the parameter to be inverted, the Huber- Markov random-field constructs a neighborhood system, which further acts as the vertical and lateral constraints. We use a quadratic Huber edge penalty function within the layer to suppress noise and a linear one on the edges to avoid a fuzzy result. The effectiveness of our method is proved by inverting the synthetic data without and with noises. The relationship between the adopted constraints and the inversion results is analyzed as well.展开更多
Background/aim： Currently, the liver is cold-preserved at 0-4 ℃ for experimental and clinical purposes. Here, we investigated whether milder hypothermia during the initial phase of the preservation period was benefi...Background/aim： Currently, the liver is cold-preserved at 0-4 ℃ for experimental and clinical purposes. Here, we investigated whether milder hypothermia during the initial phase of the preservation period was beneficial for liver viability upon reperfusion. Methods： In the first set of experiments, rat livers were preserved either conventionally in clinically used histidine-trypthopan-ketoglutarate （HTK） solution （Group A： 45 min and Group B： 24 h） or by slow cooling HTK solution （from 13 ℃ to 3 ℃） during the initial 45 min of preservation （Group C： 24 h）. In the second set of experiments, additional groups of livers were evaluated： Group BB-preservation according to Group B and Group CC-preservation according to Group C. Further, some livers were preserved at 13 ℃ for 24 h. Livers were then reperfused using a blood-free perfusion model. Results： Bile production was approximately 2-fold greater in Group C compared to Group B. Alanine transaminase （ALT） and aspartate transaminase （AST） release into perfusate were 2-3-fold higher in Group B compared to Group C. No significant differences were found in ALT and AST release between Group C and Group A. Livers in Group CC compared to Group BB exhibited significantly lower portal resistance, greater oxygen consumption and bromosulfophthalein excretion into bile and lower lactate dehydrogenase （LDH） release into perfusate. Histological evaluation of tissue sections in Group BB showed parenchymal dystrophy of hepatocytes, while dystrophy ofhepatocytes was absent in Group CC. Livers preserved at 13 ℃ for 24 h exhibited severe ischemic injury Conclusion： These results suggest that the conventional way of liver preservation is not suitable at least for rat livers and that slow cooling of HTK solution during the initial phase of cold storage can improve liver viability during reperfusion.展开更多
In more recent times, health-related quality of life (HRQOL) measurements have formed an important part of as- sessing the quality of routine care in general practice. For a measure to have clinical usefulness it must...In more recent times, health-related quality of life (HRQOL) measurements have formed an important part of as- sessing the quality of routine care in general practice. For a measure to have clinical usefulness it must not only be valid, appro- priate, reliable, responsive, and capable of being interpreted, but it must also be simple, fast to complete, easy to score, and provide useful clinical data. The Two-step method of choosing appropriate measures is introduced. Then through comparison of generic instruments with disease-specific instruments, we can conclude that sometimes a combination of generic and disease-specific HRQOL measures may be more appropriate for monitoring changes in a patient’s health status due to an intervention.展开更多
基金National Natural Science Foundation of Shanxi Province(No:19991091) and HiTech Resereh and Development Program of China (No:2004AA2Z3815)
文摘Aim The enhanced effect of Bushen (Kidney-tonifying) decoction (BS) oncultured PC12 cell proliferation and its antagonistic action on neurotoxicity induced by glutamatewere investigated by serum pharmacological method of the Chinese material medica (CMM) in vitro.Methods The effect of BS on cultured PC12 cell activity and its antagonistic action on neurotoxicityinduced by glutamate was observed by MTT method. Flow cytometry and fluorescence microscopetechniques were employed to observe the antagonistic effect of BS on early period apoptosis of PC12cells induced by glutamate. Results The serum with BS was able to enhance activity of PC12 cells andexert antagonistic effect on glutamate-induced neurotoxicity. Meanwhile, these beneficial effectsproduced by BS were found to be the strongest in 20% concentration of in serum BS. Moreover, it caninhibit apoptosis of PC12 cells induced by glutamate , which occurs in the early period. ConclusionBS may exert a potential neuroprotective effect.
文摘The increasing scale and complexity of power systems require high performance and high reliability of power system protection.Protective relaying based on directional comparison with power line carrier or microwave channels is the most suitable protection scheme for long distance EHV transmission lines and is widely used in power systems.The key element of such protection is a directional relay used to discriminate the fault direction.In order to overcome the disadvantages of conventional directional relays,the authors of this paper put forward the directional comparison carrier protection based on the artificial neural network(ANN).The protection is extensively tested using electromagnetic transient program (EMTP) under various electric power system operating and fault conditions.It is proved that the directional comparison carrier protection based on ANN,which can recognize various fault patterns of the protected transmission line(such as fault direction,fault phases etc.)correctly in any kind of operating and fault conditions and the whole process,is satisfactory for EHV transmission line protection.
基金Support by Key Project in Marine Science and Technology of Guangdong (No.A20099E01)Guangdong Provincial 908 Project ( No.GD908-02-05)~~
文摘From the protection of Chinese white dolphin and living environment,and considering the industrial development in coastal areas,we put forward adjustment program for Jiangmen Chinese White Dolphin Provincial Nature Reserve. Meanwhile we analyzed the feasibility of this program by systematically focusing the effect of adjusted functional regions on Chinese white dolphin.
基金Supported by Project of the Department of Education of Fujian Province(JB13317)~~
文摘To ensure the safety of consumers, prolong the shelf life of moon cake and reduce the cost of manufacturing and marketing enterprises, the optimization of preservative formula and the shelf life of cantonese style moon cake were studied. Through single and compound formula preservative test, the results showed that potassium sorbate and sodium dehydroacetate could inhibit the microorganisms in the moon cake, but the inhibitory effect of sodium dehydroacetate was stronger than that of potassium sorbate; and the combination of potassium sorbate and sodium dehydroacetate had a synergistic effect, and the best formula is ＇potassium sorbate 0.44 g/kg mixed with sodium dehydroacetate 0.38 g/kg. A batch of moon cake was prepared with the best preservative formula, then the total number of colonies, molds and coliform group in the samples were detected by random sampling at irregular intervals. The total number of molds exceeded the limit on the 187^th d, and it indicated that the shelf life of the product was 187 d. Compared with the conventional 15-day shelf life, this research greatly prolongs the sheff life of cantonese style moon cake, and cut down the cost of moon cake enterprises. It is worthy of popularization and application.
基金The National High Technology Research and Development Program of China (863 Program), No. 2001AA216071Guangdong Health Bureau Scientific Funds, No. 2006345
文摘AIM: To compare the preservation of non-heart- beating donor (NHBD) livers in cold histidine-trytophan- ketoglutarate (HTK) solution and extracorporeal liver perfusion (ECLP). METHODS: Livers harvested from health pigs were stored for 10 h in cold HTK solution (group A, n = 4) or perfused with oxygenated autologous blood at body temperature (group B, n = 4). Both groups were then tested on the circuit for 4 h. Bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of extracorporeal livers were tested in each group. Liver tissues from each group were examined at the end of reperfusion. RESULTS: At 1, 2, 3 and 4 h after reperfusion, bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of livers in group A were statistically different from those in group B (P < 0.05 or P < 0.01). CONCLUSION: ECLP is better than HTK solution to preserve NHBD livers. ECLP can assess the graft viabilitybefore liver transplantation.
基金The National High Technology Research and Deve-lopment Program of China (863Program)(No.2002AA123031).
文摘To enhance the bandwidth efficiency of the guard interval （GI） assisted wireless communication system, an attractive scheme is proposed, which combines the functions of pilots and the GI together, so that the pilot resource used for estimating channel state is saved. Based on the proposed different known guard intervals （DKGI）, the time-domain channel estimation can be simply applied on the receiver side. After channel estimation, the receiver can employ the cyclic convolution restoring （CCR） function to reconstruct the cyclical convolution relationship between the signal and the channel, by which the receiver can also achieve good performance through the conventional 1-tap frequency domain equalization （FDE）.
文摘Single-strand breaks （SSBs） can occur in cells either directly, or indirectly following initiation of base excision repair （BER）. SSBs generally have blocked termini lacking the conventional 5＇-phosphate and 3＇-hydroxyl groups and require further processing prior to DNA synthesis and ligation. XRCC1 is devoid of any known enzymatic activity, but it can physically interact with other proteins involved in all stages of the overlapping SSB repair and BER pathways, including those that conduct the rate-limiting end-tailoring, and in many cases can stimulate their enzymatic activities. XRCC1^-/- mouse fibroblasts are most hypersensitive to agents that produce DNA lesions repaired by monofunctional glycosylase-initiated BER and that result in formation of indirect SSBs. A requirement for the deoxyribose phosphate lyase activity of DNA polymerase β （pol β） is specific to this pathway, whereas pol β is implicated in gap-filling during repair of many types of SSBs. Elevated levels of strand breaks, and diminished repair, have been demonstrated in MMS- treated XRCC1^-/-, and to a lesser extent in pol β^-/- cell lines, compared with wild-type cells. Thus a strong correlation is observed between cellular sensitivity to MMS and the ability of cells to repair MMS-induced damage. Exposure of wild-type and polβ^-/- cells to an inhibitor of PARP activity dramatically potentiates MMS-induced cytotoxicity. XRCC1^-/- cells are also sensitized by PARP inhibition demonstrating that PARP-mediated poly（ADP-ribosyl）ation plays a role in modulation of cytotoxicity beyond recruitment of XRCC 1 to sites of DNA damage.
文摘A class of high resolution positivity preserving Boltzmann type difference schemes for one and two dimensional Euler equations is studied. First, the relation between Boltzmann and Euler equations is analyzed. By using a kind of special interpolation, the high resolution Boltzmann type difference scheme is constructed. Finally, numerical tests show that the schemes are effective and useful.
基金Under the auspices of National Natural Science Foundation of China(No.41230745,41171156)
文摘Poverty reduction and environmental protection are two global tasks for sustainable development.The study perspective has changed over time,from narrowly focusing on poverty reduction to comprehensively strengthening human-welfare.We reviewed key references targeting the theoretical content and practical approach relying on poverty,environment protection and ecosystem services.We discussed the contradicting views on the relationship of poverty and environmental degradation,and then illustrated the study progress of a cutting-age topic-ecosystem services which pave a way to address poverty reduction and environmental protection together.At last,we investigated essential factors that affect the development and environmental protection.Considering the evolution of the concept of poverty,we found that the environment has occupied an increasing proportion in the cognizance of poverty.The relationship between poverty and environmental degradation is regional uniqueness.In practical aspect,projects based on the management and valuation assessment of ecosystem services draw researchers′attention all over the world.The appropriate scale,essential economic incentives,morality,law and social equality are key factors affecting individuals′decisions which directly relate to the sustainable development.
基金supported by the National Basic Research Program of China(973 Program)(No.2013CB228603)National Science and Technology major projects(No.2011ZX05024 and 2011ZX05010)the National Natural Science Foundation of China(No.41174119)
文摘Seismic inversion is a highly ill-posed problem, due to many factors such as the limited seismic frequency bandwidth and inappropriate forward modeling. To obtain a unique solution, some smoothing constraints, e.g., the Tikhonov regularization are usually applied. The Tikhonov method can maintain a global smooth solution, but cause a fuzzy structure edge. In this paper we use Huber-Markov random-field edge protection method in the procedure of inverting three parameters, P-velocity, S-velocity and density. The method can avoid blurring the structure edge and resist noise. For the parameter to be inverted, the Huber- Markov random-field constructs a neighborhood system, which further acts as the vertical and lateral constraints. We use a quadratic Huber edge penalty function within the layer to suppress noise and a linear one on the edges to avoid a fuzzy result. The effectiveness of our method is proved by inverting the synthetic data without and with noises. The relationship between the adopted constraints and the inversion results is analyzed as well.
基金Project supported by the Ministry of Health of the Slovak Republicunder the project of Modulation of Heat Transfer in Isolated Liver (No.2005/32-SZU-10)the VEGA Grant (No.1/1158/04),Slo-vakia
文摘Background/aim： Currently, the liver is cold-preserved at 0-4 ℃ for experimental and clinical purposes. Here, we investigated whether milder hypothermia during the initial phase of the preservation period was beneficial for liver viability upon reperfusion. Methods： In the first set of experiments, rat livers were preserved either conventionally in clinically used histidine-trypthopan-ketoglutarate （HTK） solution （Group A： 45 min and Group B： 24 h） or by slow cooling HTK solution （from 13 ℃ to 3 ℃） during the initial 45 min of preservation （Group C： 24 h）. In the second set of experiments, additional groups of livers were evaluated： Group BB-preservation according to Group B and Group CC-preservation according to Group C. Further, some livers were preserved at 13 ℃ for 24 h. Livers were then reperfused using a blood-free perfusion model. Results： Bile production was approximately 2-fold greater in Group C compared to Group B. Alanine transaminase （ALT） and aspartate transaminase （AST） release into perfusate were 2-3-fold higher in Group B compared to Group C. No significant differences were found in ALT and AST release between Group C and Group A. Livers in Group CC compared to Group BB exhibited significantly lower portal resistance, greater oxygen consumption and bromosulfophthalein excretion into bile and lower lactate dehydrogenase （LDH） release into perfusate. Histological evaluation of tissue sections in Group BB showed parenchymal dystrophy of hepatocytes, while dystrophy ofhepatocytes was absent in Group CC. Livers preserved at 13 ℃ for 24 h exhibited severe ischemic injury Conclusion： These results suggest that the conventional way of liver preservation is not suitable at least for rat livers and that slow cooling of HTK solution during the initial phase of cold storage can improve liver viability during reperfusion.
文摘In more recent times, health-related quality of life (HRQOL) measurements have formed an important part of as- sessing the quality of routine care in general practice. For a measure to have clinical usefulness it must not only be valid, appro- priate, reliable, responsive, and capable of being interpreted, but it must also be simple, fast to complete, easy to score, and provide useful clinical data. The Two-step method of choosing appropriate measures is introduced. Then through comparison of generic instruments with disease-specific instruments, we can conclude that sometimes a combination of generic and disease-specific HRQOL measures may be more appropriate for monitoring changes in a patient’s health status due to an intervention.
