The method of regularization factor selection determines stability and accuracy of the regularization method. A formula of regularization factor was proposed by analyzing the relationship between the improved SVD and ...The method of regularization factor selection determines stability and accuracy of the regularization method. A formula of regularization factor was proposed by analyzing the relationship between the improved SVD and regularization method. The improved SVD algorithm and regularization method could adapt to low SNR. The regularization method is better than the improved SVD in the case that SNR is below 30 and the improved SVD is better than the regularization method when SNR is higher than 30. The regularization method with the regularization factor proposed in this paper can be better applied into low SNR (5〈SNR) NMR logging. The numerical simulations and real NMR data process results indicated that the improved SVD algorithm and regularization method could adapt to the low signal to noise ratio and reduce the amount of computation greatly. These algorithms can be applied in NMR logging.展开更多
The high-frequency(HF) communication is one of essential communication methods for military and emergency application. However, the selection of communication frequency channel is always a difficult problem as the cro...The high-frequency(HF) communication is one of essential communication methods for military and emergency application. However, the selection of communication frequency channel is always a difficult problem as the crowded spectrum, the time-varying channels, and the malicious intelligent jamming. The existing frequency hopping, automatic link establishment and some new anti-jamming technologies can not completely solve the above problems. In this article, we adopt deep reinforcement learning to solve this intractable challenge. First, the combination of the spectrum state and the channel gain state is defined as the complex environmental state, and the Markov characteristic of defined state is analyzed and proved. Then, considering that the spectrum state and channel gain state are heterogeneous information, a new deep Q network(DQN) framework is designed, which contains multiple sub-networks to process different kinds of information. Finally, aiming to improve the learning speed and efficiency, the optimization targets of corresponding sub-networks are reasonably designed, and a heterogeneous information fusion deep reinforcement learning(HIF-DRL) algorithm is designed for the specific frequency selection. Simulation results show that the proposed algorithm performs well in channel prediction, jamming avoidance and frequency channel selection.展开更多
Pregnane and Xenobiotic Receptor (PXR; or Steroid and Xenobiotic Receptor, SXR), a new member of the nuclear receptor superfamily, is thought to modulate a network of genes that are involved in xenobiotic metabolism a...Pregnane and Xenobiotic Receptor (PXR; or Steroid and Xenobiotic Receptor, SXR), a new member of the nuclear receptor superfamily, is thought to modulate a network of genes that are involved in xenobiotic metabolism and elimination. To further explore the role of PXR in body’s homeostatic mechanisms, we for the first time, report successful prokary- otic expression and purification of full-length PXR and preparation of polyclonal antibody against the whole protein. The full-length cDNA encoding a 434 amino acids protein was sub-cloned into prokaryotic expression vector, pET-30b and transformed into E. coli BL21(DE3) cells for efficient over expression. The inclusion body fraction, containing the expressed recombinant protein, was purified first by solubilizing in sarcosine extraction buffer and then by affinity column chromatography using Ni-NTA His-Bind matrix. The efficacy of anti-PXR antibody was confirmed by immunocytology, Western blot analysis, EMSA and immunohistochemistry. The antibody obtained was capable of detecting human and mouse PXR with high specificity and sensitivity. Immunofluorescence staining of COS-1 cells transfected with human or mouse PXR showed a clear nuclear localization. Results from immunohistochemistry showed that level of PXR in liver sections is immunologically detectable in the nuclei. Similar to exogenously transfected PXR, Western blot analysis of cell extract from HepG2 and COLO320DM cells revealed a major protein band for endogenous PXR having the expected molecular weight of 50 kDa. Relevance of other immunodetectable bands with reference to PXR isoforms and current testimony are evaluated. Advantages of antibody raised against full-length PXR protein for functional characterization of receptor is discussed and its application for clinical purposes is envisaged.展开更多
Polyketides have been widely used clinically due to their significant biological activities, but the needed structural and functional diversity cannot be achieved by common chemical synthetic methods. The tool of comb...Polyketides have been widely used clinically due to their significant biological activities, but the needed structural and functional diversity cannot be achieved by common chemical synthetic methods. The tool of combinatorial biosynthesis provides the possibility to produce "unnatural" natural drugs, which has achieved initial success. This paper provides an overview for the strategies of combinatorial biosynthesis in producing the structural and functional diversity of polyketides, including the redesign of metabolic flow, polyketide synthase(PKS) engineering, and PKS post-translational modification. Although encouraging progress has been made in the last decade, challenges still exist regarding the rational combinatorial biosynthesis of polyketides. In this review, the perspectives of polyketide combinatorial biosynthesis are also discussed.展开更多
Isoamylene from the Fischer-Tropsch syncrude can be transformed to valuable fuel oxygenate additives through an equilibrium limited etherification reaction with methanol. A reactive distillation process is established...Isoamylene from the Fischer-Tropsch syncrude can be transformed to valuable fuel oxygenate additives through an equilibrium limited etherification reaction with methanol. A reactive distillation process is established to increase isoamylene conversion. Facing the challenge of improving product purity at the same time, an equilibrium stage model based design methodology is proposed and illustrated step-by-step for converting the Fischer-Tropsch C_5 olefins to tert-amyl methyl ether(TAME) process by using Aspen Plus. Under the guide of the proposed methodology, the design leads to a TAME product purity of higher than 95% and an isoamylene conversion of higher than 90%. The etherification kinetics over Amberlyst 35 is also studied within a temperature range of 60 ℃ to 75 ℃ to shed more light on the feasibility of process development. The methodology provides an effective reactive distillation column design to achieve the target reactant conversion and product purity simultaneously.展开更多
The study of snow and ice melt (SIM) is important in water-scarce arid regions for the assessment of water supply and quality. These studies involve unique difficulties, especially in the calibration of hydro-models...The study of snow and ice melt (SIM) is important in water-scarce arid regions for the assessment of water supply and quality. These studies involve unique difficulties, especially in the calibration of hydro-models because there is no direct way to continuously measure the SIM at hydrostations. The recursive digital filter (RDF) and the isotopic hydro-geochemical method (IHM) were coupled to separate the SIM from eight observed series of alpine streamflows in northwestern China. Validation of the calibrated methods suggested a good capture of the SIM characteristics with fair accuracy in both space and time. Applications of the coupled methods in the upper reaches of the Hei River Basin (HRB) suggested a double peak curve of the SIM fraction to streamflow for the multi-component recharged (MCR) rivers, while a single peak curve was suggested for the rainfall-dominant recharged (RDR) rivers. Given inter-annual statistics of the separation, both types of the alpine rivers have experienced an obvious decrease of SIM since 196os. In the past 10 years, the SIM in the two types of rivers has risen to the levels of the 1970s, but has remained lower than the level of the 1960s. The study provided a considerable evidence to quantify the alpine SIMbased on the separation of observed data series at gauge stations. Application of the coupled method could be helpful in the calibration and validation of SIM-related hydro-models in alpine regions.展开更多
文摘The method of regularization factor selection determines stability and accuracy of the regularization method. A formula of regularization factor was proposed by analyzing the relationship between the improved SVD and regularization method. The improved SVD algorithm and regularization method could adapt to low SNR. The regularization method is better than the improved SVD in the case that SNR is below 30 and the improved SVD is better than the regularization method when SNR is higher than 30. The regularization method with the regularization factor proposed in this paper can be better applied into low SNR (5〈SNR) NMR logging. The numerical simulations and real NMR data process results indicated that the improved SVD algorithm and regularization method could adapt to the low signal to noise ratio and reduce the amount of computation greatly. These algorithms can be applied in NMR logging.
基金supported by Guangxi key Laboratory Fund of Embedded Technology and Intelligent System under Grant No. 2018B-1the Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province under Grant No. BK20160034+1 种基金the National Natural Science Foundation of China under Grant No. 61771488, No. 61671473 and No. 61631020in part by the Open Research Foundation of Science and Technology on Communication Networks Laboratory
文摘The high-frequency(HF) communication is one of essential communication methods for military and emergency application. However, the selection of communication frequency channel is always a difficult problem as the crowded spectrum, the time-varying channels, and the malicious intelligent jamming. The existing frequency hopping, automatic link establishment and some new anti-jamming technologies can not completely solve the above problems. In this article, we adopt deep reinforcement learning to solve this intractable challenge. First, the combination of the spectrum state and the channel gain state is defined as the complex environmental state, and the Markov characteristic of defined state is analyzed and proved. Then, considering that the spectrum state and channel gain state are heterogeneous information, a new deep Q network(DQN) framework is designed, which contains multiple sub-networks to process different kinds of information. Finally, aiming to improve the learning speed and efficiency, the optimization targets of corresponding sub-networks are reasonably designed, and a heterogeneous information fusion deep reinforcement learning(HIF-DRL) algorithm is designed for the specific frequency selection. Simulation results show that the proposed algorithm performs well in channel prediction, jamming avoidance and frequency channel selection.
文摘Pregnane and Xenobiotic Receptor (PXR; or Steroid and Xenobiotic Receptor, SXR), a new member of the nuclear receptor superfamily, is thought to modulate a network of genes that are involved in xenobiotic metabolism and elimination. To further explore the role of PXR in body’s homeostatic mechanisms, we for the first time, report successful prokary- otic expression and purification of full-length PXR and preparation of polyclonal antibody against the whole protein. The full-length cDNA encoding a 434 amino acids protein was sub-cloned into prokaryotic expression vector, pET-30b and transformed into E. coli BL21(DE3) cells for efficient over expression. The inclusion body fraction, containing the expressed recombinant protein, was purified first by solubilizing in sarcosine extraction buffer and then by affinity column chromatography using Ni-NTA His-Bind matrix. The efficacy of anti-PXR antibody was confirmed by immunocytology, Western blot analysis, EMSA and immunohistochemistry. The antibody obtained was capable of detecting human and mouse PXR with high specificity and sensitivity. Immunofluorescence staining of COS-1 cells transfected with human or mouse PXR showed a clear nuclear localization. Results from immunohistochemistry showed that level of PXR in liver sections is immunologically detectable in the nuclei. Similar to exogenously transfected PXR, Western blot analysis of cell extract from HepG2 and COLO320DM cells revealed a major protein band for endogenous PXR having the expected molecular weight of 50 kDa. Relevance of other immunodetectable bands with reference to PXR isoforms and current testimony are evaluated. Advantages of antibody raised against full-length PXR protein for functional characterization of receptor is discussed and its application for clinical purposes is envisaged.
基金supported by the Major Research Plan of Tianjin(No.16YFXTSF00460)
文摘Polyketides have been widely used clinically due to their significant biological activities, but the needed structural and functional diversity cannot be achieved by common chemical synthetic methods. The tool of combinatorial biosynthesis provides the possibility to produce "unnatural" natural drugs, which has achieved initial success. This paper provides an overview for the strategies of combinatorial biosynthesis in producing the structural and functional diversity of polyketides, including the redesign of metabolic flow, polyketide synthase(PKS) engineering, and PKS post-translational modification. Although encouraging progress has been made in the last decade, challenges still exist regarding the rational combinatorial biosynthesis of polyketides. In this review, the perspectives of polyketide combinatorial biosynthesis are also discussed.
基金financial support from the National High Technology Research and Development Program 863(2011AA05A204)National Natural Science Foundation of China(U1361202)
文摘Isoamylene from the Fischer-Tropsch syncrude can be transformed to valuable fuel oxygenate additives through an equilibrium limited etherification reaction with methanol. A reactive distillation process is established to increase isoamylene conversion. Facing the challenge of improving product purity at the same time, an equilibrium stage model based design methodology is proposed and illustrated step-by-step for converting the Fischer-Tropsch C_5 olefins to tert-amyl methyl ether(TAME) process by using Aspen Plus. Under the guide of the proposed methodology, the design leads to a TAME product purity of higher than 95% and an isoamylene conversion of higher than 90%. The etherification kinetics over Amberlyst 35 is also studied within a temperature range of 60 ℃ to 75 ℃ to shed more light on the feasibility of process development. The methodology provides an effective reactive distillation column design to achieve the target reactant conversion and product purity simultaneously.
基金supported by the following grants:National Key Research and Development Program of China (Grant No. 2009CB421306)the NSFC Project (Grant Nos. 41001014, 51209119) NSFC Projects (Grant Nos. 41240002, 91225301)+1 种基金the NSFC Key Project (Grant No. 91125010)the MAIRS Project funded by the NASA LCLUC Program (Grant No. NNX08AH50G)
文摘The study of snow and ice melt (SIM) is important in water-scarce arid regions for the assessment of water supply and quality. These studies involve unique difficulties, especially in the calibration of hydro-models because there is no direct way to continuously measure the SIM at hydrostations. The recursive digital filter (RDF) and the isotopic hydro-geochemical method (IHM) were coupled to separate the SIM from eight observed series of alpine streamflows in northwestern China. Validation of the calibrated methods suggested a good capture of the SIM characteristics with fair accuracy in both space and time. Applications of the coupled methods in the upper reaches of the Hei River Basin (HRB) suggested a double peak curve of the SIM fraction to streamflow for the multi-component recharged (MCR) rivers, while a single peak curve was suggested for the rainfall-dominant recharged (RDR) rivers. Given inter-annual statistics of the separation, both types of the alpine rivers have experienced an obvious decrease of SIM since 196os. In the past 10 years, the SIM in the two types of rivers has risen to the levels of the 1970s, but has remained lower than the level of the 1960s. The study provided a considerable evidence to quantify the alpine SIMbased on the separation of observed data series at gauge stations. Application of the coupled method could be helpful in the calibration and validation of SIM-related hydro-models in alpine regions.