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HPLC无孔径载体柱用于快速分离蛋白质和多肽 被引量:6
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作者 张丽华 屠红 +1 位作者 吴高德 夏其昌 《高技术通讯》 CAS CSCD 1995年第2期45-47,共3页
介绍了一种新型的高效液相色谱柱(无孔径载体柱)的结构特点及其在蛋白质、多肽及遗传工程产品分析中的应用。在相同的洗脱条件下,与常规柱相比,无孔径载体柱高效、快速,特别是它能分离分子量较大和疏水性较强的蛋白质。此外,就无... 介绍了一种新型的高效液相色谱柱(无孔径载体柱)的结构特点及其在蛋白质、多肽及遗传工程产品分析中的应用。在相同的洗脱条件下,与常规柱相比,无孔径载体柱高效、快速,特别是它能分离分子量较大和疏水性较强的蛋白质。此外,就无孔径载体柱在使用过程中对仪器的要求作了阐述,还讨论了温度对柱效的影响以及灵敏度与梯度时间的关系等。 展开更多
关键词 无孔径载体柱 遗传工程 蛋白 多肽 分离 HPLC
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近场扫描干涉无孔径显微术
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作者 王佳 刘秀梅 李达成 《光学技术》 EI CAS CSCD 1997年第6期12-15,共4页
本文介绍了国外研究的一种新型近场扫描无孔径干涉显微镜的原理及结构。这种显微镜的分辨率可达到1nm,开辟了在亚纳米尺度成像和进行光谱分析的可能性,特别是在化学和生物学研究领域具有潜在的应用前景。
关键词 近场光学 干涉显微镜 无孔径显微术 显微镜
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反射式无孔径近场Raman研究(英文)
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作者 WX Sun ZW Shen 《光散射学报》 2002年第4期245-251,共7页
近场扫描光学显微技术与Raman光谱技术的结合能够在纳米尺度下提供化学 /结构信息 ,这对很多应用都是至关重要的 ,比如硅器件 ,纳米器件 ,量子点及生物样品单分子研究。本文报导了采用无孔径探针的近场Raman研究。我们的系统有两大特征 ... 近场扫描光学显微技术与Raman光谱技术的结合能够在纳米尺度下提供化学 /结构信息 ,这对很多应用都是至关重要的 ,比如硅器件 ,纳米器件 ,量子点及生物样品单分子研究。本文报导了采用无孔径探针的近场Raman研究。我们的系统有两大特征 :1 近场Raman的增强是通过金属探针上的银镀层实现的 ,无需样品准备 ;2 系统在反射模式下工作 ,适用于任何样品。这两点对实际应用是至关重要的。我们首次在实际硅器件上用 1秒积分时间获得了 1维近场Raman映射和 2维近场Raman图象。我们首次展示了由于积分时间短 ,该技术可用于成象用途。因此 ,这是近场扫描Raman研究中的一次巨大进步。此外 ,我们系统中采用的金属探针可同时用于AFM及电学特性成象 ,比如电阻 ,电容 ,这些是器件应用中的重要参数。 展开更多
关键词 拉曼光谱 近场光学 无孔径探针
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A Novel Approach to Synthesizing Porous ZnO Films: Inorganic Chelating Sol-Gel Method 被引量:1
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作者 杨立荣 靳正国 +1 位作者 步邵静 程志捷 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2004年第3期241-246,共6页
Porous ZnO films are synthesized by inorganic chelating sol-gel method,which is a novel sol-gel technique using zinc nitrate as starting materials and citric acid as the chelating reagent.The crystal structure,surface... Porous ZnO films are synthesized by inorganic chelating sol-gel method,which is a novel sol-gel technique using zinc nitrate as starting materials and citric acid as the chelating reagent.The crystal structure,surface morphology,porous and optical properties of the deposited films are investigated.X-ray diffraction pattern analysis shows that crystal structure of the ZnO films is hexagonal wurtzite.Scanning electron microscopy (SEM) shows that the ZnO film is porous.The curve of pore size distribution has two peak values at about 2.02nm and 4.97nm and BET surface area of the ZnO film is 27.57m2/g.In addition,the transmittance spectrum gives a high transmittance of 85% in the visible region and optical bandgap of the ZnO film (fired at 500℃) is 3.25eV. 展开更多
关键词 porous ZnO film inorganic chelating sol-gel method pore size distribution PROPERTIES
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眼镜、放大镜、显微镜及显微术
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《中国光学》 EI CAS 1998年第5期98-98,共1页
TH742 98053478近场扫描干涉无孔径显微术=Near—field opticalscanning interferometric apertureless mi-croseopy[刊,中]/王佳,刘秀梅,李达成(清华大学精密仪器系。北京(100084))∥光学技术。—1997,(6).—12—15介绍了国外研究的一... TH742 98053478近场扫描干涉无孔径显微术=Near—field opticalscanning interferometric apertureless mi-croseopy[刊,中]/王佳,刘秀梅,李达成(清华大学精密仪器系。北京(100084))∥光学技术。—1997,(6).—12—15介绍了国外研究的一种新型近场扫描无孔径干涉显微镜的原理及结构。这种显微镜的分辨率可达到1nm,开辟了在亚纳米尺度成象和进行光谱分析的可能性,特别是在光学和生物学研究领域具有潜在的应用前景。图6参3(方舟)TH742.64 展开更多
关键词 显微术 电子显微镜 光子扫描隧道显微镜 无孔径 近场扫描 干涉显微镜 光谱分析 强衰场 精密仪器 纳米尺度
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Research on 3D marine electromagnetic interferometry with synthetic sources for suppressing the airwave interference 被引量:1
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作者 张建国 武欣 +2 位作者 齐有政 黄玲 方广有 《Applied Geophysics》 SCIE CSCD 2013年第4期373-383,510,共12页
In order to suppress the airwave noise in marine controlled-source electromagnetic (CSEM) data, we propose a 3D deconvolution (3DD) interferometry method with a synthetic aperture source and obtain the relative an... In order to suppress the airwave noise in marine controlled-source electromagnetic (CSEM) data, we propose a 3D deconvolution (3DD) interferometry method with a synthetic aperture source and obtain the relative anomaly coefficient (RAC) of the EM field reflection responses to show the degree for suppressing the airwave. We analyze the potential of the proposed method for suppressing the airwave, and compare the proposed method with traditional methods in their effectiveness. A method to select synthetic source length is derived and the effect of the water depth on RAC is examined via numerical simulations. The results suggest that 3DD interferometry method with a synthetic source can effectively suppress the airwave and enhance the potential of marine CSEM to hydrocarbon exploration. 展开更多
关键词 marine CSEM reflection response airwave synthetic aperture method 3D deconvolution interferometry up- and down-going field decomposition
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UWB SAR RFI SUPPRESSION BASED ON REGION OF SUPPORT IN 2-D WAVENUMBER DOMAIN 被引量:2
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作者 Jin Tian Zhou Zhimin Song Qian Chang Wenge 《Journal of Electronics(China)》 2007年第4期503-508,共6页
Radio Frequency Interference (RFI) degrades the quality of focused Ultra-WideBand Syn- thetic Aperture Radar (UWB SAR) images. From both the theoretical analysis and real data valida- tion, it is concluded that target... Radio Frequency Interference (RFI) degrades the quality of focused Ultra-WideBand Syn- thetic Aperture Radar (UWB SAR) images. From both the theoretical analysis and real data valida- tion, it is concluded that target echo and RFI have different Region Of Support (ROS) in 2-D fast- time wavenumber and aperture wavenumber domain. Consequently, a novel adaptive filter is pro- posed according to the Wiener optimum criterion on the distinct ROS characteristics of target echo and RFI. Compared with the notch filter and the Least Mean Square (LMS) adaptive filter in previ- ous literatures, the proposed method is more computationally efficient with satisfactory suppression results. In terms of Signal-to-Interference Ratio Improvement (SIRI) and processing time, the per- formance of the proposed adaptive filter is verified with the field data collected with a UWB SAR system. 展开更多
关键词 Ultra-WideBand (UWB) Synthetic Aperture Radar (SAR) Radio Frequency Interference (RFI) suppression Wiener criterion
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Effect of Fiber Properties on Nonwovens' Pore Structures with Fractal Geometry Analysis
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作者 杨旭红 李栋高 《Journal of Donghua University(English Edition)》 EI CAS 2005年第4期103-108,共6页
Nonwovens' pore structures are very important to their mechanical and physical properties. And the pore structures are influenced by the fiber properties and fibers arrangement in web. In this paper, the fractal geom... Nonwovens' pore structures are very important to their mechanical and physical properties. And the pore structures are influenced by the fiber properties and fibers arrangement in web. In this paper, the fractal geometry, in combination with computer image anaysis, is used to express the irregularity of pore size distribution in nonwovens, and the effect of fiber properties on fractal dimension of pore size distribution is discussed by using simulated images which are composed of nonlinear staple fibers. The results show that the fiber properties, such as crimp, diameter, angular distribution, and especially the number of fibers prominently influence the pore structure. 展开更多
关键词 NONWOVENS fiber properties fractal dimension of pore size distribution simulated images.
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针尖增强拉曼光谱术原理与系统设计关键 被引量:6
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作者 王瑞 郝凤欢 +3 位作者 张明倩 王佳 郁鉴源 田芊 《激光与光电子学进展》 CSCD 北大核心 2010年第3期58-67,共10页
最近10年,针尖增强拉曼光谱技术(TERS)以其极高的空间分辨率和探测灵敏度受到了人们的重视。针尖增强拉曼光谱技术为纳米科学与生命科学等学科的研究提供了强有力的工具,使得纳米尺度光谱学研究成为现实。简述了针尖增强拉曼光谱技术的... 最近10年,针尖增强拉曼光谱技术(TERS)以其极高的空间分辨率和探测灵敏度受到了人们的重视。针尖增强拉曼光谱技术为纳米科学与生命科学等学科的研究提供了强有力的工具,使得纳米尺度光谱学研究成为现实。简述了针尖增强拉曼光谱技术的基本原理,着重讨论了针尖增强拉曼光谱系统的结构、设计方法和评价指标。介绍了针尖增强拉曼光谱技术的最新应用和研究进展,并对目前面对的前沿问题和今后的发展方向进行了分析。 展开更多
关键词 光谱学 针尖增强拉曼光谱 近场光学 无孔径近场光学显微镜
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Determining the oil weighting factor of oil-water emulsion based on the fractional Weierstrass scattering model
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作者 LI Yang TAO Ran SHENG XinQing 《Science China Earth Sciences》 SCIE EI CAS 2014年第8期1780-1789,共10页
The formation of oil-water emulsion often occurs when oil is spilled into the ocean. Oil weighting factor of oil-water emulsion is one of the most important parameters for emergent oil-spill microwave monitoring. A ne... The formation of oil-water emulsion often occurs when oil is spilled into the ocean. Oil weighting factor of oil-water emulsion is one of the most important parameters for emergent oil-spill microwave monitoring. A new method is proposed here to evaluate the oil weighting factor based on fractional Weierstrass scattering model. By using the proposed method, we analyze the Uninhabited Aerial Vehicle Synthetic Aperture Radar(UAVSAR) L-band fully polarimetric data acquired during 2010 Deepwater Horizon oil spill disaster event in the Gulf of Mexico. The result shows that our method performs well in evaluating oil weighting factor of oil-covered area. 展开更多
关键词 oil spill Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR) Deepwater Horizon (DWH) fractionalWeierstrass scattering model
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