岷县当归无机元素分析及重金属安全研究

目的 分析岷县当归中Li、Be、Na、Mg、Al、K、Ca等28种无机元素的含量及重金属安全状况,建立岷县当归无机元素指纹图谱.方法 采用超级微波-电感耦合等离子体质谱法测定岷县当归中无机元素含量.结果 岷县当归中28种无机元素均有检出,各元素含量差异很大.平均含量最高的是K(106722.680 mg/kg),最低的为Cd(0.021 mg/kg);重金属Cu、As、Cd、Hg、Pb的含量分别为4.941、0.540、0.021、0.028、0.749 mg/kg占《药用植物及制剂进口绿色行业标准》中相应元素限量(Cu≤20.000 mg/kg,As≤2.000 mg/kg,Cd≤0.300 mg/kg,Hg≤0.200 mg/kg,Pb≤5.000 mg/kg)的24.7%、27.2%、7.0%、14.0%、15.0%;28种无机元素含量分布具有相似峰形.结论 目前,岷县当归重金属积累较少,建立的无机元素指纹图谱,从无机元素角度进一步完善岷县当归指纹图谱数据库,为岷县当归的产地溯源技术的建立提供谱图支持.

Wavy Distribution of Trace Elements in Phytolacca Samples from Different Areas

To study the distribution of 25 elements, i.e. Be, Cd, Ce, Cr, Cu, Dy, Er,Eu, Gd, Ge, Ho, La, Lu, Mo, Nd, Pb, Pr, Sm, Sr, Tb, Tl, Tm, Y, Yb and Zn in Phytolacca, atraditional Chinese medicinal herb, collected from nine areas of P. R. China. Methods Twenty-fiveelements in Phytolacca including essential elements, toxic elements and rare earth elements intraditional Chinese medicinal herbs from different areas were analyzed by ICP-MS. Results The 25elements in Phytolacca were determined by ICP-MS under optimized conditions. The detection limitswere 0.003 -0.71 ng·mL^(-1). The recoveries were 88% - 118% . The relative standard deviations ofthe measurements were 1.7% - 13.3%. Conclusion The determined concentrations of elements inPhytolacca acinosa Roxb vary from one area to another; however, the distribution tendency ofelements in all the samples is similar. The distribution tendency of rare earth elements inPhytolacca acinosa Roxb is consistent with that in nature.

Inorganic Elements in Tuber of Chinese Herbal Medicine Radix Pseudostellariae from Guizhou Province

By analyzing the contents of inorganic elements in Radix Pseudostellariae from Guizhou Province, the aim was to assess the present quality of Radix Pseu- dostellariae, set limited standards of heavy metals, establish element fingerprints, and find out the characteristic elements. ICP-MS was used to measure the content of inorganic elements and map the element fingerprints. Additionally, WM/T2-2004 was applied to evaluate the quality of heavy metal elements, and the characteristic elements were determined by principal component analysis. The results showed that the contents of inorganic elements in Radix Pseudostellariae were between 0.057 and 959 mg/kg with the coefficients of variation ranging from 0.134 to 1.478, and the contents of Cd, As, Pb, and Hg were below the Standard of WM/T2-2004 in 90% of Radix Pseudostellariae. The standard limits of heavy metals in Radix Pseu- dostellariae were Cr≤6.5 mg/kg, Cu≤10 mg/kg, As≤2.0 mg /kg, Cd≤0.3 mg/kg, Hg≤0.15 mg/kg, and Pb≤4.0 mg/kg. The features of the inorganic eJements finger- prints could provide theoretical basis of identifying the quality of Radix Pseudostel- lariae and distinguishing Radix Pseudostellariae from other Chinese herbal medicines. The characteristic inorganic elements of Radix Pseudostellariae were found to be Cd, Cu, Co, Zn, Fe, Ca, Mg, and AI. Radix Pseudostellariae from Guizhou Province contained abundant inorganic elements, and the contents of heavy metals were below the evaluation criterion. The study provided a reference for the future development of the limiting values of heaw metals in Radix Pseudostellariae.

Inorganic Elemental Determinations of Marine Traditional Chinese Medicine Meretricis concha from Jiaozhou Bay:The construction of Inorganic Elemental Fingerprint Based on Chemometric Analysis

The goal of this paper is to explore the relationship between the inorganic elemental fingerprint and the geographical origin identification ofMeretricis concha, which is a commonly used marine traditional Chinese medicine （TCM） for the treatment of asthma and scald bums. For that, the inorganic elemental contents ofMeretricis concha from five sampling points in Jiaozhou Bay have been determined by means of inductively coupled plasma optical emission spectrometry, and the comparative investigations based on the contents of 14 inorganic elements （A1, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Se and Zn） of the samples from Jiaozhou Bay and the previous reported Rushan Bay were performed. It has been found that the samples from the two bays are ap- proximately classified into two kinds using hierarchical cluster analysis, and a four-factor model based on principle component analysis could explain approximately 75% of the detection data, also linear discriminant analysis can be used to develop a prediction model to distinguish the samples from Jiaozhou Bay and Rushan Bay with accuracy of about 93%. The results of the present investi- gation suggested that the inorganic elemental fingerprint based on the combination of the measured elemental content and chemom- etric analysis is a promising approach for verifying the geographical origin ofMeretricis concha, and this strategy should be valuable for the authenticity discrimination of some marine TCM.

基于无机元素分析的当归烟熏加工工艺优选

目的:探讨不同烟熏条件加工的当归药材中15种无机元素的含量及分布特征,确定最佳烟熏加工工艺。方法:采用ICP-MS测定31个烟熏当归样品中8种重金属元素含量,AAS测定其他7种无机元素含量,利用IBM SPSS 21.0及SIMCA-P 11.5软件进行统计分析。结果:不同烟熏条件加工的当归药材中无机元素特征性指纹表现为K>Na>Cr>Cu的趋势;烟熏温度是影响烟熏当归药材中无机元素分布的最关键因素;最佳烟熏加工工艺为50℃烟熏8 h。结论:调控温度和时间进行烟熏加工的当归药材安全性良好。