目的通过实时无标记细胞分析法(real time cell analysis,RTCA)与MTT法的比较研究,探讨RTCA法用于医疗器械细胞毒性常规检测的可行性。方法首先,通过筛选细胞接种密度建立基于L929细胞的RTCA细胞毒性检测方法。然后,使用不同浓度的DMSO...目的通过实时无标记细胞分析法(real time cell analysis,RTCA)与MTT法的比较研究,探讨RTCA法用于医疗器械细胞毒性常规检测的可行性。方法首先,通过筛选细胞接种密度建立基于L929细胞的RTCA细胞毒性检测方法。然后,使用不同浓度的DMSO稀释液、标准对照材料浸提液、56批真实世界的医疗器械产品,从简单到复杂分别比较RTCA法与MTT法用于细胞毒性检测结果的一致性,并且汇总多次试验的阴性对照和阳性对照结果,比较两种方法的可重复性。结果RTCA法检测不同浓度的DMSO稀释液没有剂量效应关系,检测标准对照材料浸提液与MTT法结果完全一致并高度相关(R=0.9898),检测56批医疗器械产品与MTT法结果一致率为80.4%,仅中度相关(R=0.6183)。RTCA法的可重复性不比MTT法好。结论RTCA法能有效检测医疗器械细胞毒性,但是部分产品可能因溶出物干扰检测结果而不适用。RTCA法作为一种新型的医疗器械细胞毒性检测方法的适用性还需要进一步研究。展开更多
A bidirectional labeling method was established to distinguish the proportions of HCO3- and CO2 utiliza- tion pathways of microalgae in Lake Hongfeng. The method was based on microalgae cultured in a medium by adding ...A bidirectional labeling method was established to distinguish the proportions of HCO3- and CO2 utiliza- tion pathways of microalgae in Lake Hongfeng. The method was based on microalgae cultured in a medium by adding equal concentrations of NaH13CO3 with different 613C values simultaneously. The inorganic carbon sources were quantified according to the stable carbon isotope composition in the treated microalgae. The effects of extracellular carbonic anhydrase （CAex） on the HCO3 and CO2 utilization pathways were distinguished using acetazolamide, a potent membrane-impermeable carbonic anhydrase inhibitor. The results show utilization of the added HCO3- was only 8% of the total carbon sources in karst lake. The proportion of the HCO3- utilization path- way was 52% of total inorganic carbon assimilation. Therefore, in the natural water of the karst area, the microalgae used less bicarbonate that preexisted in the aqueous medium than CO2 derived from the atmosphere. CAex increased the utilization of inorganic carbon from the atmosphere. The microalgae with CAex had greater carbon sequestration capacity in this karst area.展开更多
A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles(PNA/Ag NPs) has been initially proposed for specific recognition of m RNA.Making use of the controlled silver nanoparticles aggrega...A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles(PNA/Ag NPs) has been initially proposed for specific recognition of m RNA.Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc m RNA detection can be achieved. Moreover, the PNA/Ag NPs platform can undergo color change in response to target c-Myc m RNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.展开更多
文摘目的通过实时无标记细胞分析法(real time cell analysis,RTCA)与MTT法的比较研究,探讨RTCA法用于医疗器械细胞毒性常规检测的可行性。方法首先,通过筛选细胞接种密度建立基于L929细胞的RTCA细胞毒性检测方法。然后,使用不同浓度的DMSO稀释液、标准对照材料浸提液、56批真实世界的医疗器械产品,从简单到复杂分别比较RTCA法与MTT法用于细胞毒性检测结果的一致性,并且汇总多次试验的阴性对照和阳性对照结果,比较两种方法的可重复性。结果RTCA法检测不同浓度的DMSO稀释液没有剂量效应关系,检测标准对照材料浸提液与MTT法结果完全一致并高度相关(R=0.9898),检测56批医疗器械产品与MTT法结果一致率为80.4%,仅中度相关(R=0.6183)。RTCA法的可重复性不比MTT法好。结论RTCA法能有效检测医疗器械细胞毒性,但是部分产品可能因溶出物干扰检测结果而不适用。RTCA法作为一种新型的医疗器械细胞毒性检测方法的适用性还需要进一步研究。
基金supported by the National Natural Sciences Foundation of China (U1612441)Foundation of Guizhou Province ([2014] 2131)Doctor Foundation of Guizhou Normal University (0514014)
文摘A bidirectional labeling method was established to distinguish the proportions of HCO3- and CO2 utiliza- tion pathways of microalgae in Lake Hongfeng. The method was based on microalgae cultured in a medium by adding equal concentrations of NaH13CO3 with different 613C values simultaneously. The inorganic carbon sources were quantified according to the stable carbon isotope composition in the treated microalgae. The effects of extracellular carbonic anhydrase （CAex） on the HCO3 and CO2 utilization pathways were distinguished using acetazolamide, a potent membrane-impermeable carbonic anhydrase inhibitor. The results show utilization of the added HCO3- was only 8% of the total carbon sources in karst lake. The proportion of the HCO3- utilization path- way was 52% of total inorganic carbon assimilation. Therefore, in the natural water of the karst area, the microalgae used less bicarbonate that preexisted in the aqueous medium than CO2 derived from the atmosphere. CAex increased the utilization of inorganic carbon from the atmosphere. The microalgae with CAex had greater carbon sequestration capacity in this karst area.
基金the National Natural Science Foundation of China (21305058, 21205056, 21075058 and 21503104)Tai-Shan Scholar Research Fund of Shandong Province
文摘A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles(PNA/Ag NPs) has been initially proposed for specific recognition of m RNA.Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc m RNA detection can be achieved. Moreover, the PNA/Ag NPs platform can undergo color change in response to target c-Myc m RNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.
