The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lip...The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.展开更多
Water-saving irrigation methods have been increasingly used for vegetable cultivation in greenhouse or plastic film house. However, there is limited information concerning the effect of water-saving irrigation methods...Water-saving irrigation methods have been increasingly used for vegetable cultivation in greenhouse or plastic film house. However, there is limited information concerning the effect of water-saving irrigation methods on soil phosphorus （P） behavior. In this experiment, drip and subsurface irrigation methods were applied, with furrow irrigation method as control, in Mollic Gleysols. Soil P distribution throughout the depth was significantly affected by irrigation methods. Total, Olsen, organic and inorganic P contents were greater in the topsoil （0-10 and 10-20 cm） than in the subsoil （20-30, 30-40, 40-50 and 50-60 cm）. The Olsen P content throughout 0-60 cm layer under drip and subsurface irrigation treatments was lower than that under the furrow irrigation treatment. However, the total, organic and inorganic P contents from 20 to 60 cm under drip irrigation treatment were higher than or close to those under furrow irrigation treatment, but were lower under subsurface irrigation treatment than under furrow irrigation treatment. Under subsurface irrigation treatment, the contents of total, organic and inorganic P at the 0-10 cm layer were 78.0%, 1.3% and 3.7% greater than those at the 10-20 cm layer, respectively. But Olsen P content at the 10-20 cm layer was 5.7% larger than that at the 0-10 cm layer. These suggested that soil P behavior could be manipulated by soil water management to some extent. 在展开更多
基金National Natural Science Foundation of China(No.29876031)
文摘The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.
基金Supported by the Liaoning Provincial Education Commission,China(No.413101206)
文摘Water-saving irrigation methods have been increasingly used for vegetable cultivation in greenhouse or plastic film house. However, there is limited information concerning the effect of water-saving irrigation methods on soil phosphorus （P） behavior. In this experiment, drip and subsurface irrigation methods were applied, with furrow irrigation method as control, in Mollic Gleysols. Soil P distribution throughout the depth was significantly affected by irrigation methods. Total, Olsen, organic and inorganic P contents were greater in the topsoil （0-10 and 10-20 cm） than in the subsoil （20-30, 30-40, 40-50 and 50-60 cm）. The Olsen P content throughout 0-60 cm layer under drip and subsurface irrigation treatments was lower than that under the furrow irrigation treatment. However, the total, organic and inorganic P contents from 20 to 60 cm under drip irrigation treatment were higher than or close to those under furrow irrigation treatment, but were lower under subsurface irrigation treatment than under furrow irrigation treatment. Under subsurface irrigation treatment, the contents of total, organic and inorganic P at the 0-10 cm layer were 78.0%, 1.3% and 3.7% greater than those at the 10-20 cm layer, respectively. But Olsen P content at the 10-20 cm layer was 5.7% larger than that at the 0-10 cm layer. These suggested that soil P behavior could be manipulated by soil water management to some extent. 在
