Objectives To evaluate the relationship between microdeletion or mutation on the Y chromosome and Chinese patients with idiopathic azoospermia and severe oligozoospermia and to establish a molecular detection method....Objectives To evaluate the relationship between microdeletion or mutation on the Y chromosome and Chinese patients with idiopathic azoospermia and severe oligozoospermia and to establish a molecular detection method.Methods Microdeletion or mutation detection at the AZFa (sY84 and USP9Y), AZFb, AZFc/DAZ and SRY regions of the Y chromosome. Seventy-three azoospermia and 28 severe oligozoospermia patients were evaluated using PCR and PCR-SSCP techniques.Results Twelve of 101 patients (12%) with the AZFc/DAZ microdeletion were found, including 8 with azoospermia (11%) and 4 with severe oligozoospermia (14.3%), and 1 patient had a AZFb and AZFc/DAZ double deletion. No deletions in the AZFa or SRY regions were found. No deletions in AZFa, AZFb, AZFc/DAZ or SRY regions were found in 60 normal men who had produced one or more children.Conclusions Microdeletion on the Y chromosome, especially at its AZFc/DAZ regions, may be a major cause of azoospermia and severe oligozoospermia leading to male infertility in China. It is recommended that patients have genetic counseling and microdeletion detection on the Y chromosome before intracytoplasmic sperm injection.展开更多
A k-colouring （not necessarily proper） of vertices of a graph is called acyclic, if for every pair of distinct colours i and j the subgraph induced by the edges whose endpoints have colours i and j is acyclic. We co...A k-colouring （not necessarily proper） of vertices of a graph is called acyclic, if for every pair of distinct colours i and j the subgraph induced by the edges whose endpoints have colours i and j is acyclic. We consider acyclie k-eolourings such that each colour class induces a graph with a given （hereditary） property. In particular, we consider aeyclic k-eolourings in which each colour class induces a graph with maximum degree at most t, which are referred to as acyclic t-improper k-colourings. The acyelic t-improper chromatic number of a graph G is the smallest k for which there exists an acyclic t-improper k-colouring of G. We focus on acyclic colourings of graphs with maximum degree 4. We prove that 3 is an upper bound for the acyclic 3-improper chromatic number of this class of graphs. We also provide a non-trivial family of graphs with maximum degree 4 whose acyclic 3-improper chromatic number is at most 2, namely, the graphs with maximum average degree at most 3. Finally, we prove that any graph G with A（G） ≤ 4 can be acyelically coloured with 4 colours in such a way that each colour class induces an acyclic graph with maximum degree at most 3.展开更多
The magnetic properties and magnetocaloric effects of amorphous and crystalline Gd55Co35Ni10 ribbons are investigated.A main phase with a Ho 12 Co 7-type monoclinic structure(space group P21/c) and a minor phase with ...The magnetic properties and magnetocaloric effects of amorphous and crystalline Gd55Co35Ni10 ribbons are investigated.A main phase with a Ho 12 Co 7-type monoclinic structure(space group P21/c) and a minor phase with a Ho4Co3-type hexagonal structure(space group P63/m) are obtained for crystalline ribbon after annealing.The amorphous ribbons order ferromagnetically and undergo a second-order transition at 192 K.For crystalline Gd55Co35Ni10 ribbons,two magnetic phase transitions occur at 158 and 214 K,respectively.The peak value of-△SM under a field change of 0-5 T is 6.5 J/kg K at 192 K for amorphous Gd55Co35Ni10 ribbons.A relatively large magnetic entropy change(～5.0 J/kg K) under a field change of 0-5 T for the crystalline Gd55Co35Ni10 ribbons is obtained in the temperature interval range of 154-214 K.The large platform of magnetic entropy change and the negligible thermal/magnetic hysteresis loss mean the crystalline Gd55Co35Ni10 compound can satisfy the requirement of the Ericsson-type refrigerator working in the temperature range from 154K to 214K.展开更多
文摘Objectives To evaluate the relationship between microdeletion or mutation on the Y chromosome and Chinese patients with idiopathic azoospermia and severe oligozoospermia and to establish a molecular detection method.Methods Microdeletion or mutation detection at the AZFa (sY84 and USP9Y), AZFb, AZFc/DAZ and SRY regions of the Y chromosome. Seventy-three azoospermia and 28 severe oligozoospermia patients were evaluated using PCR and PCR-SSCP techniques.Results Twelve of 101 patients (12%) with the AZFc/DAZ microdeletion were found, including 8 with azoospermia (11%) and 4 with severe oligozoospermia (14.3%), and 1 patient had a AZFb and AZFc/DAZ double deletion. No deletions in the AZFa or SRY regions were found. No deletions in AZFa, AZFb, AZFc/DAZ or SRY regions were found in 60 normal men who had produced one or more children.Conclusions Microdeletion on the Y chromosome, especially at its AZFc/DAZ regions, may be a major cause of azoospermia and severe oligozoospermia leading to male infertility in China. It is recommended that patients have genetic counseling and microdeletion detection on the Y chromosome before intracytoplasmic sperm injection.
基金supported by the Minister of Science and Higher Education of Poland(Grant No.JP2010009070)
文摘A k-colouring （not necessarily proper） of vertices of a graph is called acyclic, if for every pair of distinct colours i and j the subgraph induced by the edges whose endpoints have colours i and j is acyclic. We consider acyclie k-eolourings such that each colour class induces a graph with a given （hereditary） property. In particular, we consider aeyclic k-eolourings in which each colour class induces a graph with maximum degree at most t, which are referred to as acyclic t-improper k-colourings. The acyelic t-improper chromatic number of a graph G is the smallest k for which there exists an acyclic t-improper k-colouring of G. We focus on acyclic colourings of graphs with maximum degree 4. We prove that 3 is an upper bound for the acyclic 3-improper chromatic number of this class of graphs. We also provide a non-trivial family of graphs with maximum degree 4 whose acyclic 3-improper chromatic number is at most 2, namely, the graphs with maximum average degree at most 3. Finally, we prove that any graph G with A（G） ≤ 4 can be acyelically coloured with 4 colours in such a way that each colour class induces an acyclic graph with maximum degree at most 3.
基金supported by the Guangdong Provincial Science and Technology Program(Grant Nos.2010B050300008,2009B090300273 and 2007B010600043)the Guangzhou Municipal Science and Technology Program(Grant No.12F582080022)+1 种基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry (Grant No.x2clB7120290)the Fundamental Research Funds for the Central Universities(Grant Nos.2011ZM0014 and 2012ZZ0013)
文摘The magnetic properties and magnetocaloric effects of amorphous and crystalline Gd55Co35Ni10 ribbons are investigated.A main phase with a Ho 12 Co 7-type monoclinic structure(space group P21/c) and a minor phase with a Ho4Co3-type hexagonal structure(space group P63/m) are obtained for crystalline ribbon after annealing.The amorphous ribbons order ferromagnetically and undergo a second-order transition at 192 K.For crystalline Gd55Co35Ni10 ribbons,two magnetic phase transitions occur at 158 and 214 K,respectively.The peak value of-△SM under a field change of 0-5 T is 6.5 J/kg K at 192 K for amorphous Gd55Co35Ni10 ribbons.A relatively large magnetic entropy change(～5.0 J/kg K) under a field change of 0-5 T for the crystalline Gd55Co35Ni10 ribbons is obtained in the temperature interval range of 154-214 K.The large platform of magnetic entropy change and the negligible thermal/magnetic hysteresis loss mean the crystalline Gd55Co35Ni10 compound can satisfy the requirement of the Ericsson-type refrigerator working in the temperature range from 154K to 214K.
