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改变灭菌包外包装对延长无菌周期的可行性研究
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作者 聂淑娟 王梅新 +3 位作者 阿依夏木.斯马衣 马槟娜 李海燕 季萍 《新疆医学》 2002年第2期89-90,共2页
高压蒸汽灭菌法是对临床耐高温器械物品进行灭菌处理的一种常用且可靠的方法,但是经此方法灭菌的物品有效期仅有1~2周,我院多年来一直以1周作为衡量物品过期的标准,然而这一标准对临床一些必备而使用率较低的器械包来讲,不仅会因反复... 高压蒸汽灭菌法是对临床耐高温器械物品进行灭菌处理的一种常用且可靠的方法,但是经此方法灭菌的物品有效期仅有1~2周,我院多年来一直以1周作为衡量物品过期的标准,然而这一标准对临床一些必备而使用率较低的器械包来讲,不仅会因反复清洗、高压而增加损耗,而且在很大程度上造成了医院资源的浪费.据此我们进行了认真大胆的尝试,即在原有灭菌包包装的基础上套一层无菌全封闭聚乙烯塑料袋,并与传统包装法之间进行实验性对比,结果令人满意,现介绍如下. 展开更多
关键词 包外包装 无菌周期 可行性研究
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Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice 被引量:10
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作者 Yuan-fei ZHOU Xian-yin ZHANG Qing-zhong XUE 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2011年第6期436-447,共12页
Pei'ai64S, an indica sterile variety with photoperiod and thermo-sensitive genic male sterile (PTGMS) genes, has been widely exploited for commercial seed production for "two-line" hybrid rice in China. One PTGMS... Pei'ai64S, an indica sterile variety with photoperiod and thermo-sensitive genic male sterile (PTGMS) genes, has been widely exploited for commercial seed production for "two-line" hybrid rice in China. One PTGMS gene from Pei'ai64S, pmsl(t), was mapped by a strategy of bulked-extreme and recessive-class approach with simple sequence repeat (SSR) and insert and deletion (In-Del) markers. Using linkage analysis for the F2 mapping population consisting of 320 completely male sterile individuals derived from a cross between Pei'ai64S and 93-11 (indica restorer) lines, the pmsl(t) gene was delimited to the region between the RM21242 (0.2 cM) and YF11 (0.2 cM) markers on the short arm of chromosome 7. The interval containing the pmsl(t) locus, which was co-segregated with RM6776, is a 101.1 kb region based on the Nipponbare rice genome. Fourteen predicted loci were found in this region by the Institute for Genomic Research (TIGR) Genomic Annotation. Based on the function of the locus LOC_Os07g12130 by bioinformatics analysis, it is predicted to encode a protein containing a Myb-like DNA-binding domain, and may process the transcript with thermosensory response. The reverse transcription-polymerase chain reaction (RT-PCR) results revealed that the mRNA levels of LOC_Os07g12130 were altered in different photoperiod and temperature treatments. Thus, the LOC_Os07g12130 locus is the most likely candidate gene for pmsl(t). These results may facilitate not only using the molecular marker assisted selection of PTGMS genes, but also cloning of the pmsl(t) gene itself. 展开更多
关键词 Super hybrid rice pmsl(t) Photoperiod and thermo-sensitive male sterile gene Genetic mapping
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