鱼腥草组织培养中材料灭菌的研究

采用不同的灭菌措施,包括不同的灭菌剂、浓度,对鱼腥草的带芽茎段进行灭菌处理,研究其污染率和污染速率的变化,寻找建立无菌培养系最适宜的方法。结果表明:最适宜的灭菌措施为:外植体茎+70%酒精浸30s+0.1%升汞10-12min+青霉素+链霉素15min。

美国山核桃组培中材料灭菌的研究

采用不同的灭菌措施,包括不同的灭菌剂、浓度和处理顺序,对美国山核桃带芽茎段进行灭菌处理,研究其污染率和污染速率的变化,寻找建立无菌培养系最适宜的方法。结果表明,外植体材料最适宜的灭菌处理方法为:先将外植体在70%酒精中浸30s,再将其用0.2%升汞液(加数滴吐温80)浸15min,无菌水冲洗10次,最后接种。培养基中添加适量活性炭,且前期暗培养是提高材料无菌率的有利保障。另外,培养基中加入青霉素、链霉素各100mg·L-1可明显提高材料的无菌率。

不同营养条件下竹叶眼子菜NH_4^+-N吸收动力学的初步研究

以竹叶眼子菜(Potamogeton malaianus)无菌系种苗为试验材料,研究了不同水体营养浓度水平(低营养:TN0.213 mg.L-1,TP 0.0093 mg.L-1;中营养:TN 0.71 mg.L-1,TP 0.031 mg.L-1;高营养:TN 7.1 mg.L-1,TP0.31 mg.L-1)对其生长与NH4+-N的吸收动力学参数的影响。结果表明,不同浓度水体营养对竹叶眼子菜生长的影响较小,而NH4+-N的吸收动力学参数有显著差异。竹叶眼子菜在高、中和低营养培养条件下的NH4+-N最大吸收速率Vmax分别为41.1、29.1、21.1μmol.g-1.h-1,米氏常数Km分别为0.356、0.306、0.122 mmol.L-1。竹叶眼子菜营养吸收动力学与其生长环境关系紧密,在低浓度生长环境中时,竹叶眼子菜可以通过降低Km值来提高对营养离子的亲和力以满足营养需求;在高浓度生长环境中,该植物通过增大吸收潜力来适应高营养。

Study on Tissue Culture and Effective Clone Establishment of Hemistepta lyrata Bunge

[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions needed in calluses induction and differentiation, adventitious bud differentiation and radication, test tube seedling cutting and transplantation were studied. [ Result] The results showed that the optimum medium for granulated calluses induction from tender stem was MS ＋ BA 0.3 mg/L ＋2,4-D 1 -1.5 mg/L, for granulated calluses and adventitious bud differentiation was MS ＋ AgNO31.5 mg/L ＋ BA 0.4 mg/L ＋ NAA 0.1 mg/L. 1/2 MS ＋ IAA 0.6 mg/L was suitable for test tube seedling rooting and regeneration, and cinder was used as transplantation and cutting substrate. [ Conclusion]This study will provide the scientific reference for choosing the feasible medium in tissue culture of Hemistepta lyrata Bunge.

Study on Shoot Tip Culture of Illciaceae Ornamental Plants

[Objective] This study aimed to establish a technology system for tissue culture and rapid propagation of Illciaceae ornamental plants. [Method] Effects of medium components and anti-browning agents on the survival and growth of shoot tips were investigated by using apical buds of IItciaceae plant Haierlian as experiment material and MS as basic medium. [Result] The results showed that apical buds at the early germination period in spring were the most suitable explants for tissue culture of IIIciaceae plant Haierlian. Sterilization with 0.1% HgCI2 for 6 min achieved the best effect, while conventional surface-sterilization with ethanol would affect the survival of explants. The optimal medium for primary culture was MS-D （with modifications in major elements and organic components） ＋ anti-browning agents （equa~ volume） ＋ 2.0 mg/L of 6-BA ＋ 0.5 mg/L of NAA. The optimal subculture medi- um was MS-F （with modifications in inorganic and organic components） ＋ anti-brown- ing agents （equal volume） ＋ 2.0 mg/L of 6-BA ＋ 0.1 mg/L of NAA. [Conclusion] This study laid the foundation for establishment of tissue culture and rapid propagation technology system for Haierlian.

辽细辛组培快繁技术研究

辽细辛是道地中药材,传统繁育方式繁殖系数低,无法满足市场需求。因此,进行了辽细辛组培快繁技术研究试验。结果表明,使用MS+2.5 mg/L6-BA+0.3 mg/LNAA的培养基诱导辽细辛愈伤组织效果较好,以MS+1.0 g/L6-BA+0.1 mg/LNAA为培养基能更好地诱导出辽细辛的不定芽;1/2MS+0.4 mg/LNAA+20 g/L蔗糖是较适合辽细辛的生根培养基。