In present paper, one of the T-DNA insertional embryonic lethal mutant of Arabidopsis is identifled and designated as acd mutant. The embryo development of this mutant is arrested in globular stage. The cell division ...In present paper, one of the T-DNA insertional embryonic lethal mutant of Arabidopsis is identifled and designated as acd mutant. The embryo development of this mutant is arrested in globular stage. The cell division pattern is abnormal during early embryogenesis and results in disturbed cellular differentiation. Most of mutant embryos are finally degenerated and aborted in globular stage. However, a few of them still can germinate in agar plate and produce seedlings with shorter hypocotyl and distorted shoot meristem. To understand the molecular basis of the phenotype of this mutant, the joint fragment of T-DNA/plant DNA is isolated by plasmid rescue and Dig-labeled as probe for cDNA library screening. According to the sequence analysis and similarity searching, a 936bp cDNA sequence (EMBL accession#: Y12555) from selected positive clone shows a 99.8 % (923/925bp) sequence homology with Alanyl-tRNA Synthetase (A1aRS) gene of Arabidopsis thaliana. Furthermore, the data of in sitll hybridization experiment indicate that the expression of AlaRS gene is weak in early embryogenesis and declines along with globular embryo ’development’ in this mutant.Accordingly, the reduced expression of AlaRS gene maybe closely related to the morphological changes in early embryogenesis of this lethal mutant.展开更多
Bucky ball(Buc)is involved in germ plasm(GP)assembly during early zebrafish development by regulating GP mRNA expression via an unknown mechanism.The present study demonstrates that an m^(6)A reader Igf2bp3 interacts ...Bucky ball(Buc)is involved in germ plasm(GP)assembly during early zebrafish development by regulating GP mRNA expression via an unknown mechanism.The present study demonstrates that an m^(6)A reader Igf2bp3 interacts and colocalizes with Buc in the GP.Similar to the loss of Buc,the genetic deletion of maternal igf2bp3 in zebrafish leads to abnormal GP assembly and insufficient germ cell specification,which can be partially restored by the injection of igf2 bp3 mRNA.Igf2bp3 binds to m^(6)A-modified GPorganizer and GP mRNAs in an m^(6)A-dependent manner and prevents their degradation.These findings indicate that the functions of Igf2bp3,a direct effector protein of Buc,in GP mRNA expression and GP assembly involve m^(6)A-dependent regulation;these results emphasize a critical role of m^(6)A modification in the process of GP assembly.展开更多
文摘In present paper, one of the T-DNA insertional embryonic lethal mutant of Arabidopsis is identifled and designated as acd mutant. The embryo development of this mutant is arrested in globular stage. The cell division pattern is abnormal during early embryogenesis and results in disturbed cellular differentiation. Most of mutant embryos are finally degenerated and aborted in globular stage. However, a few of them still can germinate in agar plate and produce seedlings with shorter hypocotyl and distorted shoot meristem. To understand the molecular basis of the phenotype of this mutant, the joint fragment of T-DNA/plant DNA is isolated by plasmid rescue and Dig-labeled as probe for cDNA library screening. According to the sequence analysis and similarity searching, a 936bp cDNA sequence (EMBL accession#: Y12555) from selected positive clone shows a 99.8 % (923/925bp) sequence homology with Alanyl-tRNA Synthetase (A1aRS) gene of Arabidopsis thaliana. Furthermore, the data of in sitll hybridization experiment indicate that the expression of AlaRS gene is weak in early embryogenesis and declines along with globular embryo ’development’ in this mutant.Accordingly, the reduced expression of AlaRS gene maybe closely related to the morphological changes in early embryogenesis of this lethal mutant.
基金supported by the National Key R&D Program of China(2018YFD0901201 and 2019YFA0802801)China Agricultural Research System(CARS-46)+3 种基金the National Natural Science Foundation of China(31870820)the Innovative Research Group Program of Hubei Province(2020CFA017)the Medical Science Advancement Program of Wuhan University(TFJC2018004)the Fundamental Research Funds for the Central Universities(2042019kf0207)。
文摘Bucky ball(Buc)is involved in germ plasm(GP)assembly during early zebrafish development by regulating GP mRNA expression via an unknown mechanism.The present study demonstrates that an m^(6)A reader Igf2bp3 interacts and colocalizes with Buc in the GP.Similar to the loss of Buc,the genetic deletion of maternal igf2bp3 in zebrafish leads to abnormal GP assembly and insufficient germ cell specification,which can be partially restored by the injection of igf2 bp3 mRNA.Igf2bp3 binds to m^(6)A-modified GPorganizer and GP mRNAs in an m^(6)A-dependent manner and prevents their degradation.These findings indicate that the functions of Igf2bp3,a direct effector protein of Buc,in GP mRNA expression and GP assembly involve m^(6)A-dependent regulation;these results emphasize a critical role of m^(6)A modification in the process of GP assembly.
