目的:建立旱田草药材的定性定量方法,考察广西不同产地、不同采收时节的药材质量。方法:采用TLC法对旱田草进行定性鉴别,展开剂为乙酸丁酯-甲醇-甲酸-水(6∶1.5∶1.5∶1);采用HPLC法对旱田草中的毛蕊花糖苷进行含量测定,使用Waters Sun ...目的:建立旱田草药材的定性定量方法,考察广西不同产地、不同采收时节的药材质量。方法:采用TLC法对旱田草进行定性鉴别,展开剂为乙酸丁酯-甲醇-甲酸-水(6∶1.5∶1.5∶1);采用HPLC法对旱田草中的毛蕊花糖苷进行含量测定,使用Waters Sun Fire C18色谱柱(4.6 mm!250 mm,5μm),柱温35℃,以甲醇-0.4%磷酸溶液(32∶68)为流动相,流速1.0 m L·min^(-1),检测波长334 nm;参照中国药典2010年版一部测定水分、灰分和浸出物。结果:薄层色谱中斑点清晰,分离度好;毛蕊花糖苷质量浓度在2.002~40.04μg·m L^(-1)(r=1.000 0)范围内线性关系良好,加样回收率为98.61%(RSD=1.7%,n=6);所测的12批样品含量在0.196~16.76 mg·g^(-1)之间。结论:本文建立的方法可用于瑶药旱田草的质量控制。展开更多
为探究des(rhamnosyl)verbascoside体外抗乙型肝炎病毒(HBV)的活性作用和机制,本研究以des(rhamnosyl)verbascoside为实验药物对HepG2.2.15细胞进行干预,实验分为药物干预组和对照组,采用串联质谱标签(Tandem Mass Tag,TMT)蛋白质组学...为探究des(rhamnosyl)verbascoside体外抗乙型肝炎病毒(HBV)的活性作用和机制,本研究以des(rhamnosyl)verbascoside为实验药物对HepG2.2.15细胞进行干预,实验分为药物干预组和对照组,采用串联质谱标签(Tandem Mass Tag,TMT)蛋白质组学方法对提取的总蛋白进行分析。结果表明,共筛选得到300个差异表达蛋白,其中有109个上调蛋白,191个下调蛋白。基因本体论(Gene Ontology,GO)分析结果显示,差异蛋白主要参与DNA复制(DNA replication)、鞘糖脂代谢(Glycosphingolipid metabolic process)、细胞增殖(Cell proliferation)、寡糖分解代谢(Oligosaccharide catabolic process)等生物学过程,以及DNA聚合酶活性(DNA polymerase activity)、丝氨酸型羧肽酶活性(Serine type carboxypeptidase activity)、DNA引物酶活性(DNA primase activity)等分子功能。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析结果显示,差异蛋白主要参与细胞代谢(Metabolism)、遗传信息传导(Genetic information processing)、生物系统通路(Organismal systems)等相关信号通路。亚细胞定位分析表明,差异蛋白大多定位在细胞质和细胞核。本研究共筛选出13个与抗HBV密切相关的蛋白。通过定量蛋白组学初步揭示des(rhamnosyl)verbascoside可能通过增加HGF、SORT1、MAN2B1,减少PRIM1、PRIM2、POLA1、POLD3、POLD2、POLD1、POLE、ERCC2、LAMC1、SDC1等蛋白表达来起到体外抗HBV的作用。展开更多
[Objective] The aim was to explore the prevention effects of pyrazosul-furon-ethyl·quinclorac 50% WP on stem and leaf of dry direct seeded rice. [Method] Totaling 7 drug doses were set in the test and the effect...[Objective] The aim was to explore the prevention effects of pyrazosul-furon-ethyl·quinclorac 50% WP on stem and leaf of dry direct seeded rice. [Method] Totaling 7 drug doses were set in the test and the effects were observed 5, 10, 15 and 30 d after drug application, with the remained weeds surveyed 15 and 30 d after drug application. [Result] The test showed pyrazosulfuron-ethyl·quinclorac 50%WP was safe to rice as treatment agent of stem and leaf, and rice yield increased dramatical y, with the growth rate in 2.1%-49.3%. After the drug application, the prevention effects on Echinochloa crusgal i, Ludwimlia prostrata, Monochoria vamli-nalis, and Zong grass were 92.2%, 96.6%, 93.5%, and 94.8%, and the effects on fresh weight were 94.8%, 97.1%, 93.3% and 94.9%, respectively. [Conclusion] Pyra-zosulfuron-ethyl·quinclorac 50% WP can be taken as treatment agents of stem and leaf of dry direct seeded rice.展开更多
文摘目的:建立旱田草药材的定性定量方法,考察广西不同产地、不同采收时节的药材质量。方法:采用TLC法对旱田草进行定性鉴别,展开剂为乙酸丁酯-甲醇-甲酸-水(6∶1.5∶1.5∶1);采用HPLC法对旱田草中的毛蕊花糖苷进行含量测定,使用Waters Sun Fire C18色谱柱(4.6 mm!250 mm,5μm),柱温35℃,以甲醇-0.4%磷酸溶液(32∶68)为流动相,流速1.0 m L·min^(-1),检测波长334 nm;参照中国药典2010年版一部测定水分、灰分和浸出物。结果:薄层色谱中斑点清晰,分离度好;毛蕊花糖苷质量浓度在2.002~40.04μg·m L^(-1)(r=1.000 0)范围内线性关系良好,加样回收率为98.61%(RSD=1.7%,n=6);所测的12批样品含量在0.196~16.76 mg·g^(-1)之间。结论:本文建立的方法可用于瑶药旱田草的质量控制。
文摘为探究des(rhamnosyl)verbascoside体外抗乙型肝炎病毒(HBV)的活性作用和机制,本研究以des(rhamnosyl)verbascoside为实验药物对HepG2.2.15细胞进行干预,实验分为药物干预组和对照组,采用串联质谱标签(Tandem Mass Tag,TMT)蛋白质组学方法对提取的总蛋白进行分析。结果表明,共筛选得到300个差异表达蛋白,其中有109个上调蛋白,191个下调蛋白。基因本体论(Gene Ontology,GO)分析结果显示,差异蛋白主要参与DNA复制(DNA replication)、鞘糖脂代谢(Glycosphingolipid metabolic process)、细胞增殖(Cell proliferation)、寡糖分解代谢(Oligosaccharide catabolic process)等生物学过程,以及DNA聚合酶活性(DNA polymerase activity)、丝氨酸型羧肽酶活性(Serine type carboxypeptidase activity)、DNA引物酶活性(DNA primase activity)等分子功能。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析结果显示,差异蛋白主要参与细胞代谢(Metabolism)、遗传信息传导(Genetic information processing)、生物系统通路(Organismal systems)等相关信号通路。亚细胞定位分析表明,差异蛋白大多定位在细胞质和细胞核。本研究共筛选出13个与抗HBV密切相关的蛋白。通过定量蛋白组学初步揭示des(rhamnosyl)verbascoside可能通过增加HGF、SORT1、MAN2B1,减少PRIM1、PRIM2、POLA1、POLD3、POLD2、POLD1、POLE、ERCC2、LAMC1、SDC1等蛋白表达来起到体外抗HBV的作用。
文摘[Objective] The aim was to explore the prevention effects of pyrazosul-furon-ethyl·quinclorac 50% WP on stem and leaf of dry direct seeded rice. [Method] Totaling 7 drug doses were set in the test and the effects were observed 5, 10, 15 and 30 d after drug application, with the remained weeds surveyed 15 and 30 d after drug application. [Result] The test showed pyrazosulfuron-ethyl·quinclorac 50%WP was safe to rice as treatment agent of stem and leaf, and rice yield increased dramatical y, with the growth rate in 2.1%-49.3%. After the drug application, the prevention effects on Echinochloa crusgal i, Ludwimlia prostrata, Monochoria vamli-nalis, and Zong grass were 92.2%, 96.6%, 93.5%, and 94.8%, and the effects on fresh weight were 94.8%, 97.1%, 93.3% and 94.9%, respectively. [Conclusion] Pyra-zosulfuron-ethyl·quinclorac 50% WP can be taken as treatment agents of stem and leaf of dry direct seeded rice.
