Twenty-four male Kazak sheep and 30 Xinjiang fine wool sheep at different ages were selected to investigate the development-dependent expression levels of fatty acid synthase (FAS) gene and hormone-sensitive lipase ...Twenty-four male Kazak sheep and 30 Xinjiang fine wool sheep at different ages were selected to investigate the development-dependent expression levels of fatty acid synthase (FAS) gene and hormone-sensitive lipase (HSL) gene in muscle and their effects on the contents of intramuscular fat (IMF). Longissimus dorsal muscle was sampled to measure IMF and total RNA was extracted to determine FAS and HSL mRNA expression levels by real-time PCR. The results showed that: l) The IMF content increased continuously with growth and showed significant differences (P 〈 0.05) between different age groups in male Kazak sheep, but in Xinjiang fine wool sheep there was no such difference observed. Furthermore, the IMF contents in Kazak were much higher (P 〈 0.01) than that of the other breed from day 30 to 90. 2) FAS mRNA expression level was the highest (P 〈 0.05) on day 0 in Kazak sheep and then declined with growth, in the other breed the gene showed a d‘ecline-rise-decline-rise' expression manner as the animals grew. HSL mRNA expression level had a similar model in two breeds, in Kazak sheep it was the highest on day 0 (P 〈 0.05) and in Xinjiang fine wool sheep on day 30 (P 〈 0.01), then both decreased after this term. 3) In male Kazak sheep, FAS and HSL mRNA expression level were both negatively related to IMF content (r= -0.485 (P = 0.02), r= -0.423 (P = 0.05)), and the ratio of FAS/HSL expression exhibited significantly negatively related IMF contents. In male Xinjiang sheep, there were no obvious relationship between FAS and HSL expression and IMF content (P 〉 0.05).展开更多
The solvents and substituents of two similar fluorescent sensors for cyanide, 7-diethylamino- 3-formylcoumarin (sensor a) and 7-diethylamino-3-(2-nitrovinyl)coumarin (sensor b), are proposed to account for their...The solvents and substituents of two similar fluorescent sensors for cyanide, 7-diethylamino- 3-formylcoumarin (sensor a) and 7-diethylamino-3-(2-nitrovinyl)coumarin (sensor b), are proposed to account for their distinct sensing mechanisms and experimental phenomena. The time-dependent density functional theory has been applied to investigate the ground states and the first singlet excited electronic states of the sensor as well as their possible Michael reaction products with cyanide, with a view to monitoring their geometries and photophysieal properties. The theoretical study indicates that the protic water solvent could lead to final Michael addition product of sensor a in the ground state, while the aprotic acetonitrile solvent could lead to carbanion as the final product of sensor b. Furthermore, the Michael reaction product of sensor a has been proved to have a torsion structure in its first singlet excited state. Correspondingly, sensor b also has a torsion structure around the nitrovinyl moiety in its first singlet excited state, while not in its carbanion structure. This could explain the observed strong fluorescence for sensor a and the quenching fluorescence for the sensor b upon the addition of the cyanide anions in the relevant sensing mechanisms.展开更多
To explore the thermal responses under the non-thermal equilibrium cold environmental conditions,a laboratory study was conducted in climate chamber.The local skin temperatures and thermal sensation of 20 subjects wer...To explore the thermal responses under the non-thermal equilibrium cold environmental conditions,a laboratory study was conducted in climate chamber.The local skin temperatures and thermal sensation of 20 subjects were recorded at 10 min intervals for 90 min under air temperatures of 7.4,9.1,11 and 15 °C.The results show that both local skin temperatures and mean skin temperature decrease not only with the drop of ambient air temperature but also with the exposure time.Local thermal sensation and overall the thermal sensation have the similar temperature-varying and time-varying characteristics.Predicted mean vote(PMV) model cannot correctly predict the thermal sensation under non-thermal equilibrium cold environment.The correlation between local thermal sensation and local skin temperatures shows that thermal sensation is closely related to skin temperature.Skin temperature is an effective indicator of thermal sensation.A linear relationship model between overall thermal sensation and mean skin temperature,considering both ambient temperature and exposure time,was established in the non-thermal equilibrium cold environment,which makes the evaluation of thermal sensation more objective.展开更多
We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli (type I) has been exposed to the dipolar magnetic field force (400, 800, 1200 and 1600 Gausses) which pr...We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli (type I) has been exposed to the dipolar magnetic field force (400, 800, 1200 and 1600 Gausses) which prepared locally with incubation for different period times (24, 48 and 72 hrs) at 37℃. The effects were evaluated by optical density (OD) at 600 nm determining their growth density incorporation with negative control and depending of McFarland turbidity standard (0.5), in addition to its susceptibility to various antibiotics. Results illustrate different forces of magnetic field decreased the growth rate of E. coli in particular at 24 hrs incubation comparing with unexposed or control samples. The magnetic field increased the logarithmic phase within 4-6 hrs of treatment but decreased after 16 to 18 hrs. Furthermore, changes in the antibiotic sensitivity were observed after exposure period of 6 hrs since E. coli cells became more sensitive to certain antibiotics. While after a 16 hrs exposure period, it became more resistant to the same antibiotics comparing with control groups.展开更多
An all-fiber sensor based on a cascaded optical fiber device is proposed and demonstrated, and its sensor head is composed of a core-offset Mach-Zehnder interferometer(MZI) and a long-period fiber grating(LPFG). In th...An all-fiber sensor based on a cascaded optical fiber device is proposed and demonstrated, and its sensor head is composed of a core-offset Mach-Zehnder interferometer(MZI) and a long-period fiber grating(LPFG). In the experiment, two dips shaped by the intermodulation between the interference fringe of MZI and the resonant wavelength of LPFG are monitored. Experimental results show that temperature sensitivities of two dips are 0.060 7 nm/°C and 0.056 3 pm/°C, and the refractive index(RI) sensitivities are –18.025 nm/RIU and –55.06 nm/RIU, respectively. The simultaneous measurement of the temperature and external RI is demonstrated based on the sensitive matrix. Its low fabrication cost, simple configuration and high sensitivity make this sensor have potential applications in chemical and biological sensing.展开更多
We designed a novel core-suspended capillary fiber that the core was suspended in the air hole and close to the inner surface of the capillary, and experimentally demonstrated its fabrication technology. In addition, ...We designed a novel core-suspended capillary fiber that the core was suspended in the air hole and close to the inner surface of the capillary, and experimentally demonstrated its fabrication technology. In addition, a method for linking a single mode fiber and a core-suspended fiber was proposed based on splicing and tapering at the fusion point between the two fibers. By combining with the optical time domain reflectometer technology, we constructed a distributed gas sensor system to monitor greenhouse gas based on this novel fiber.展开更多
We propose a fully distributed fusion system combining phase-sensitive optical time-domain reflectometry(Φ-OTDR) and OTDR for synchronous vibration and loss measurement by setting an ingenious frequency sweep rate(FS...We propose a fully distributed fusion system combining phase-sensitive optical time-domain reflectometry(Φ-OTDR) and OTDR for synchronous vibration and loss measurement by setting an ingenious frequency sweep rate(FSR) of the optical source. The relationships between FSR, probe pulse width and repeat period are given to balance the amplitude fluctuation of OTDR traces, the dead zone probability and the measurable frequency range of vibration events. In the experiment, we achieve synchronous vibration and loss measurement with FSR of 40 MHz/s, the proble pulse width of 100 ns and repeat rate of 0.4 ms. The fluctuation of OTDR trace is less than 0.45 dB when the signalto-noise ratio(SNR) is over 12 dB for a captured vibration event located at 9.1 km. The proposed method can be used for not only detection but also early warning of damage events in optical communication networks.展开更多
This work presented a demonstration of the potential for a fiber based cavity ring-down (CRD) using an optical time-domain reflectometer (OTDR). The OTDR was used to send the impulses down into about 20km of a sta...This work presented a demonstration of the potential for a fiber based cavity ring-down (CRD) using an optical time-domain reflectometer (OTDR). The OTDR was used to send the impulses down into about 20km of a standard single optical fiber, at the end of which the fiber cavity ring-down was placed. The OTDR measured no appreciable losses, so other CRDs multiplexed could be spliced in parallel along the same optical fiber. To demonstrate the behavior and sensitivity of the proposed configuration, a displacement sensor based on a fiber taper with a diameter of 50 μm was placed inside the fiber loop, and the induced losses were measured on the CRD signal -- a sensitivity of 11.8 + 0.5 μs/mm was achieved. The dynamic range of the sensing head used in this configuration was about 2 mm. Finally, this work was also compared with different works published in the literature.展开更多
In this paper, a model of photonic crystal temperature sensor based on crystal microcavity in a straight photonic crystal waveguide is proposed. The transmission characteristics of light in the sensor under different ...In this paper, a model of photonic crystal temperature sensor based on crystal microcavity in a straight photonic crystal waveguide is proposed. The transmission characteristics of light in the sensor under different temperatures are simulated by using finite-difference time-domain (FDTD) method. The thermal expansion and thermal-optic effects of silicon are taken into account. The results show that the resonant wavelength of microcavity increases linearly as the temperature rising. The wavelength shift along with temperature is 6.6 pm /℃.展开更多
基金National Natural Sciences Foundation of China (No. 30671503)Youth Science and Technology Innovation Foundation (No. KJ05011)SRT Program (No. 0605A09) of Nanjing Agriculture University.
文摘Twenty-four male Kazak sheep and 30 Xinjiang fine wool sheep at different ages were selected to investigate the development-dependent expression levels of fatty acid synthase (FAS) gene and hormone-sensitive lipase (HSL) gene in muscle and their effects on the contents of intramuscular fat (IMF). Longissimus dorsal muscle was sampled to measure IMF and total RNA was extracted to determine FAS and HSL mRNA expression levels by real-time PCR. The results showed that: l) The IMF content increased continuously with growth and showed significant differences (P 〈 0.05) between different age groups in male Kazak sheep, but in Xinjiang fine wool sheep there was no such difference observed. Furthermore, the IMF contents in Kazak were much higher (P 〈 0.01) than that of the other breed from day 30 to 90. 2) FAS mRNA expression level was the highest (P 〈 0.05) on day 0 in Kazak sheep and then declined with growth, in the other breed the gene showed a d‘ecline-rise-decline-rise' expression manner as the animals grew. HSL mRNA expression level had a similar model in two breeds, in Kazak sheep it was the highest on day 0 (P 〈 0.05) and in Xinjiang fine wool sheep on day 30 (P 〈 0.01), then both decreased after this term. 3) In male Kazak sheep, FAS and HSL mRNA expression level were both negatively related to IMF content (r= -0.485 (P = 0.02), r= -0.423 (P = 0.05)), and the ratio of FAS/HSL expression exhibited significantly negatively related IMF contents. In male Xinjiang sheep, there were no obvious relationship between FAS and HSL expression and IMF content (P 〉 0.05).
基金This work was supported by the National Key Basic Research Special Foundation (No.2007CB815202 and No.2009CB220010) and the National Natural Science Foundation of China (No.20833008).
文摘The solvents and substituents of two similar fluorescent sensors for cyanide, 7-diethylamino- 3-formylcoumarin (sensor a) and 7-diethylamino-3-(2-nitrovinyl)coumarin (sensor b), are proposed to account for their distinct sensing mechanisms and experimental phenomena. The time-dependent density functional theory has been applied to investigate the ground states and the first singlet excited electronic states of the sensor as well as their possible Michael reaction products with cyanide, with a view to monitoring their geometries and photophysieal properties. The theoretical study indicates that the protic water solvent could lead to final Michael addition product of sensor a in the ground state, while the aprotic acetonitrile solvent could lead to carbanion as the final product of sensor b. Furthermore, the Michael reaction product of sensor a has been proved to have a torsion structure in its first singlet excited state. Correspondingly, sensor b also has a torsion structure around the nitrovinyl moiety in its first singlet excited state, while not in its carbanion structure. This could explain the observed strong fluorescence for sensor a and the quenching fluorescence for the sensor b upon the addition of the cyanide anions in the relevant sensing mechanisms.
基金Project(50838009) supported by the National Natural Science Foundation of China
文摘To explore the thermal responses under the non-thermal equilibrium cold environmental conditions,a laboratory study was conducted in climate chamber.The local skin temperatures and thermal sensation of 20 subjects were recorded at 10 min intervals for 90 min under air temperatures of 7.4,9.1,11 and 15 °C.The results show that both local skin temperatures and mean skin temperature decrease not only with the drop of ambient air temperature but also with the exposure time.Local thermal sensation and overall the thermal sensation have the similar temperature-varying and time-varying characteristics.Predicted mean vote(PMV) model cannot correctly predict the thermal sensation under non-thermal equilibrium cold environment.The correlation between local thermal sensation and local skin temperatures shows that thermal sensation is closely related to skin temperature.Skin temperature is an effective indicator of thermal sensation.A linear relationship model between overall thermal sensation and mean skin temperature,considering both ambient temperature and exposure time,was established in the non-thermal equilibrium cold environment,which makes the evaluation of thermal sensation more objective.
文摘We studied the biological effects of different magnetic fields. Identified bacterial strain Escherichia coli (type I) has been exposed to the dipolar magnetic field force (400, 800, 1200 and 1600 Gausses) which prepared locally with incubation for different period times (24, 48 and 72 hrs) at 37℃. The effects were evaluated by optical density (OD) at 600 nm determining their growth density incorporation with negative control and depending of McFarland turbidity standard (0.5), in addition to its susceptibility to various antibiotics. Results illustrate different forces of magnetic field decreased the growth rate of E. coli in particular at 24 hrs incubation comparing with unexposed or control samples. The magnetic field increased the logarithmic phase within 4-6 hrs of treatment but decreased after 16 to 18 hrs. Furthermore, changes in the antibiotic sensitivity were observed after exposure period of 6 hrs since E. coli cells became more sensitive to certain antibiotics. While after a 16 hrs exposure period, it became more resistant to the same antibiotics comparing with control groups.
基金supported by the National High Technology Research and Development Program(No.2013AA014200)the National Natural Science Foundation of China(No.61107052)+1 种基金the Natural Science Foundation of Tianjin(No.14JCYBJC16500)the Tianjin University's Science and Technology Development Fund Projects(No.2012)
文摘An all-fiber sensor based on a cascaded optical fiber device is proposed and demonstrated, and its sensor head is composed of a core-offset Mach-Zehnder interferometer(MZI) and a long-period fiber grating(LPFG). In the experiment, two dips shaped by the intermodulation between the interference fringe of MZI and the resonant wavelength of LPFG are monitored. Experimental results show that temperature sensitivities of two dips are 0.060 7 nm/°C and 0.056 3 pm/°C, and the refractive index(RI) sensitivities are –18.025 nm/RIU and –55.06 nm/RIU, respectively. The simultaneous measurement of the temperature and external RI is demonstrated based on the sensitive matrix. Its low fabrication cost, simple configuration and high sensitivity make this sensor have potential applications in chemical and biological sensing.
基金This work was supported by the Key Laboratory Program for in-Fiber Integrated Optics of the Education Ministry of China, partially supported by the National Nature Science Foundation of China (No. 41174161) and by China Postdoctoral Science Foundation Grant (2013M531013).
文摘We designed a novel core-suspended capillary fiber that the core was suspended in the air hole and close to the inner surface of the capillary, and experimentally demonstrated its fabrication technology. In addition, a method for linking a single mode fiber and a core-suspended fiber was proposed based on splicing and tapering at the fusion point between the two fibers. By combining with the optical time domain reflectometer technology, we constructed a distributed gas sensor system to monitor greenhouse gas based on this novel fiber.
基金supported by the National Natural Science Foundation of China(Nos.61405090,61307096 and 61540017)
文摘We propose a fully distributed fusion system combining phase-sensitive optical time-domain reflectometry(Φ-OTDR) and OTDR for synchronous vibration and loss measurement by setting an ingenious frequency sweep rate(FSR) of the optical source. The relationships between FSR, probe pulse width and repeat period are given to balance the amplitude fluctuation of OTDR traces, the dead zone probability and the measurable frequency range of vibration events. In the experiment, we achieve synchronous vibration and loss measurement with FSR of 40 MHz/s, the proble pulse width of 100 ns and repeat rate of 0.4 ms. The fluctuation of OTDR trace is less than 0.45 dB when the signalto-noise ratio(SNR) is over 12 dB for a captured vibration event located at 9.1 km. The proposed method can be used for not only detection but also early warning of damage events in optical communication networks.
文摘This work presented a demonstration of the potential for a fiber based cavity ring-down (CRD) using an optical time-domain reflectometer (OTDR). The OTDR was used to send the impulses down into about 20km of a standard single optical fiber, at the end of which the fiber cavity ring-down was placed. The OTDR measured no appreciable losses, so other CRDs multiplexed could be spliced in parallel along the same optical fiber. To demonstrate the behavior and sensitivity of the proposed configuration, a displacement sensor based on a fiber taper with a diameter of 50 μm was placed inside the fiber loop, and the induced losses were measured on the CRD signal -- a sensitivity of 11.8 + 0.5 μs/mm was achieved. The dynamic range of the sensing head used in this configuration was about 2 mm. Finally, this work was also compared with different works published in the literature.
基金surpported by the National 863 Project of China (No.2007AA03Z413)the National Nature Science Foundation of China (No.60727004)the Project of Education Office of Shanxi Province of China (No.09JS041)
文摘In this paper, a model of photonic crystal temperature sensor based on crystal microcavity in a straight photonic crystal waveguide is proposed. The transmission characteristics of light in the sensor under different temperatures are simulated by using finite-difference time-domain (FDTD) method. The thermal expansion and thermal-optic effects of silicon are taken into account. The results show that the resonant wavelength of microcavity increases linearly as the temperature rising. The wavelength shift along with temperature is 6.6 pm /℃.