AIM: To compare the ligase detection reaction (LDR) and real-time PCR for detection of low abundant YMDD mutants in patients with chronic hepatitis B infection.METHODS: Mixtures of plasmids and serum samples from 52 c...AIM: To compare the ligase detection reaction (LDR) and real-time PCR for detection of low abundant YMDD mutants in patients with chronic hepatitis B infection.METHODS: Mixtures of plasmids and serum samples from 52 chronic hepatitis B patients with low abundant lamivudine-resistant mutations were tested with LDR and real-time PCR. Time required and reagent cost for both assays were evaluated.RESULTS: Real-time PCR detected 100, 50, 10, 1 and 0.1% of YIDD plasmid, whereas LDR detected 100, 50, 10, 1, 0.1, and 0.01% of YIDD plasmid, in mixtures with YMDD plasmid of 106 copies/mL. Among the 52 clinical serum samples, completely concordant results were obtained for all samples by both assays, and 39 YIDD, 9 YVDD, and 4 YIDD/YVDD were detected. Cost and time required for LDR and real-time PCR are 60/80 CNY (8/10.7 US dollars) and 4.5/2.5 h, respectively.CONCLUSION: LDR and real-time PCR are both sensitive and inexpensive methods for monitoring low abundant YMDD mutants during lamivudine therapy in patients with chronic hepatitis B. LDR is more sensitive and less expensive, while real-time PCR is more rapid.展开更多
Arboviruses represent a serious problem to public health and agriculture worldwide. Fast, accurate identification of the viral agents of arbovirus-associated disease is essential for epidemiological surveillance and l...Arboviruses represent a serious problem to public health and agriculture worldwide. Fast, accurate identification of the viral agents of arbovirus-associated disease is essential for epidemiological surveillance and laboratory investigation. We developed a cost-effective, rapid, and highly sensitive one-step "triplex RT-PCR enzyme hybridization" assay for simultaneous detections of Japanese Encephallitis virus (JEV, Flaviviridae), Getah virus (GETV, Togaviridae), and Tahyna virus (TAHV, Bunyaviridae) using three pairs of primers to amplify three target sequences in one RT-PCR reaction. The analytical sensitivity of this assay was 1 PFU/mL for JEV, 10 PFU/mL for GETV, and 10 PFU/mL for TAHV. This assay is significantly more rapid and less expensive than the traditional serological detection and single RT-PCR reaction methods. When "triplex RT-PCR enzyme hybridization" was applied to 29 cerebrospinal fluid (CSF) samples that were JEV-positive by normal RT-PCR assay, all samples were strongly positive for JEV, but negative for GETV and TAHV, demonstrating a good sensitivity, specificity, and performance at CSF specimen detection.展开更多
To solve the slow congestion detection and rate convergence problems in the existing rate control based fair data collection schemes, a new fair data collection scheme is proposed, which is named the improved scheme w...To solve the slow congestion detection and rate convergence problems in the existing rate control based fair data collection schemes, a new fair data collection scheme is proposed, which is named the improved scheme with fairness or ISWF for short. In ISWF, a quick congestion detection method, which combines the queue length with traffic changes of a node, is used to solve the slow congestion detection problem, and a new solution, which adjusts the rate of sending data of a node by monitoring the channel utilization rate, is used to solve the slow convergence problem. At the same time, the probability selection method is used in ISWF to achieve the fairness of channel bandwidth utilization. Experiment and simulation results show that ISWF can effectively reduce the reaction time in detecting congestion and shorten the rate convergence process. Compared with the existing tree-based fair data collection schemes, ISWF can achieve better fairness in data collection and reduce the transmission delay effectively, and at the same time, it can increase the average network throughput by 9.1% or more.展开更多
Based on existing low-frequency water-filled impedance tube testing facilities, which is a part of the Low Frequency Facility of the Naval Undersea Warfare Center in Beijing, an improved water-filled pulse tube method...Based on existing low-frequency water-filled impedance tube testing facilities, which is a part of the Low Frequency Facility of the Naval Undersea Warfare Center in Beijing, an improved water-filled pulse tube method is presented in this short paper. This proposed study is significantly different from the conventional pulse tube method because of the capability for a single plane damped sine pulse wave to generate in the water-filled pulse tube with a regular waveform and short duration time of about 1ms. During the generation process of the pulse, an inverse filter principle was adopted to compensate the transducer response. The effect of the characteristics of tube termination can be eliminated through the generation process of the pulse. Reflection coefficient from a water/air interface was measured to verify the proposed method. When compared with the expected theoretical values, a relatively good agreement can be obtained in the low frequency range of 500-2 000 Hz.展开更多
We label-free and real-time detected three interaction processes of antigen-antibodies,Human Immunoglobulin G(IgG),Rabbit IgG,and Mouse IgG as the targets,and Goat Anti-human IgG,Goat Anti-rabbit IgG,and Goat Anti-mou...We label-free and real-time detected three interaction processes of antigen-antibodies,Human Immunoglobulin G(IgG),Rabbit IgG,and Mouse IgG as the targets,and Goat Anti-human IgG,Goat Anti-rabbit IgG,and Goat Anti-mouse IgG as the probe,by the Oblique-incidence Reflectivity Difference(OIRD) method.The interaction dynamic curves of the OIRD signal,corresponding to the interaction processes of antigen-antibodies,are generated.The reaction times from beginning to equilibrium state are about 1800,900,and 1200 s for Human IgG,Rabbit IgG,and Mouse IgG,respectively.The experimental results demonstrate that the OIRD method not only can distinguish biomolecular interactions,but also can be used in real-time detection of interactions and dynamic processes of biomolecules.展开更多
Underground contamination by non-aqueous phase liquids (NAPLs) becomes increasingly serious. Rapid and reliable detection of contaminated zone and degree is the first step to site remediation. In this paper, diesel an...Underground contamination by non-aqueous phase liquids (NAPLs) becomes increasingly serious. Rapid and reliable detection of contaminated zone and degree is the first step to site remediation. In this paper, diesel and fine sand are used as experiment materials to investigate the applicability of using time-domain reflectometry (TDR) to detect LNAPLs contamination. The major work includes: measurement of dielectric constant and electrical conductivity for the diesel-water-air-sand mixtures; measurement of reflection waveform and dielectric constant for specimens with a diesel contaminated layer being sandwiched in sand. The experimental results show the followings: A significant decrease in both dielectric constant and electrical conductivity is observed for the diesel-water-air-sand mixtures when diesel displaces the pore water, and the content of diesel can be calculated by the model; insignificant change in dielectric properties is measured when diesel only displaces the pore gas; when the diesel contaminated sand is sandwiched between two saturated sand layers, the interfaces of the diesel contaminated layer can be identified by analyzing the reflection waveform; for field application, TDR method is valid for the case that LNAPLs seep into saturated sand layer, and the applicability of TDR method in vadose zone depends on the initial saturation of the sand layer. The findings obtained in this paper provide a guidance for the use of TDR for the field investigation of NAPLs contaminated site.展开更多
Selected Mouse IgG of 1 mg/mL as target was fabricated on microarray for 500 sample dots.Label-free and real-time reaction dynamic processes were detected between the microarrays with Goat Anti-mouse IgG of 0.02 mg/mL...Selected Mouse IgG of 1 mg/mL as target was fabricated on microarray for 500 sample dots.Label-free and real-time reaction dynamic processes were detected between the microarrays with Goat Anti-mouse IgG of 0.02 mg/mL using the obliqueincidence reflectivity difference(OIRD)method.We obtained the reaction results and the reaction dynamic curves of 500 protein dots.In addition,we also used label-free detection of protein microarrays of 10080 sample dots,including BSA and different concentrations of Mouse IgG and Rabbit IgG,by OIRD.The obtained reaction results between the protein microarray with 1 mg/mL Goat Anti-mouse IgG and 1 mg/mL Goat Anti-rabbit IgG are reported herein.Experimental results show that OIRD can be not only label-free high-throughput detection method for biological microarrays but also label-free real-time detection in the interaction processes of biomolecules.展开更多
文摘AIM: To compare the ligase detection reaction (LDR) and real-time PCR for detection of low abundant YMDD mutants in patients with chronic hepatitis B infection.METHODS: Mixtures of plasmids and serum samples from 52 chronic hepatitis B patients with low abundant lamivudine-resistant mutations were tested with LDR and real-time PCR. Time required and reagent cost for both assays were evaluated.RESULTS: Real-time PCR detected 100, 50, 10, 1 and 0.1% of YIDD plasmid, whereas LDR detected 100, 50, 10, 1, 0.1, and 0.01% of YIDD plasmid, in mixtures with YMDD plasmid of 106 copies/mL. Among the 52 clinical serum samples, completely concordant results were obtained for all samples by both assays, and 39 YIDD, 9 YVDD, and 4 YIDD/YVDD were detected. Cost and time required for LDR and real-time PCR are 60/80 CNY (8/10.7 US dollars) and 4.5/2.5 h, respectively.CONCLUSION: LDR and real-time PCR are both sensitive and inexpensive methods for monitoring low abundant YMDD mutants during lamivudine therapy in patients with chronic hepatitis B. LDR is more sensitive and less expensive, while real-time PCR is more rapid.
基金NIH Grant (2U54AI057160-06)Development Grant of State Key Laboratory for Infectious Disease Prevention and Control (2008SKLID105)
文摘Arboviruses represent a serious problem to public health and agriculture worldwide. Fast, accurate identification of the viral agents of arbovirus-associated disease is essential for epidemiological surveillance and laboratory investigation. We developed a cost-effective, rapid, and highly sensitive one-step "triplex RT-PCR enzyme hybridization" assay for simultaneous detections of Japanese Encephallitis virus (JEV, Flaviviridae), Getah virus (GETV, Togaviridae), and Tahyna virus (TAHV, Bunyaviridae) using three pairs of primers to amplify three target sequences in one RT-PCR reaction. The analytical sensitivity of this assay was 1 PFU/mL for JEV, 10 PFU/mL for GETV, and 10 PFU/mL for TAHV. This assay is significantly more rapid and less expensive than the traditional serological detection and single RT-PCR reaction methods. When "triplex RT-PCR enzyme hybridization" was applied to 29 cerebrospinal fluid (CSF) samples that were JEV-positive by normal RT-PCR assay, all samples were strongly positive for JEV, but negative for GETV and TAHV, demonstrating a good sensitivity, specificity, and performance at CSF specimen detection.
基金supported in part by the National Natural Science Foundation of China under Grants No. 61103178, No. 60803151the Research Fund for the Doctoral Program of Higher Education of China under Grant No. 20096102120045
文摘To solve the slow congestion detection and rate convergence problems in the existing rate control based fair data collection schemes, a new fair data collection scheme is proposed, which is named the improved scheme with fairness or ISWF for short. In ISWF, a quick congestion detection method, which combines the queue length with traffic changes of a node, is used to solve the slow congestion detection problem, and a new solution, which adjusts the rate of sending data of a node by monitoring the channel utilization rate, is used to solve the slow convergence problem. At the same time, the probability selection method is used in ISWF to achieve the fairness of channel bandwidth utilization. Experiment and simulation results show that ISWF can effectively reduce the reaction time in detecting congestion and shorten the rate convergence process. Compared with the existing tree-based fair data collection schemes, ISWF can achieve better fairness in data collection and reduce the transmission delay effectively, and at the same time, it can increase the average network throughput by 9.1% or more.
基金Supported by the National Natural Science Foundation of China under Grant No. 11204242 China Postdoctoral Foundation under Grant No. 2011M501477
文摘Based on existing low-frequency water-filled impedance tube testing facilities, which is a part of the Low Frequency Facility of the Naval Undersea Warfare Center in Beijing, an improved water-filled pulse tube method is presented in this short paper. This proposed study is significantly different from the conventional pulse tube method because of the capability for a single plane damped sine pulse wave to generate in the water-filled pulse tube with a regular waveform and short duration time of about 1ms. During the generation process of the pulse, an inverse filter principle was adopted to compensate the transducer response. The effect of the characteristics of tube termination can be eliminated through the generation process of the pulse. Reflection coefficient from a water/air interface was measured to verify the proposed method. When compared with the expected theoretical values, a relatively good agreement can be obtained in the low frequency range of 500-2 000 Hz.
基金supported by the National Basic Research Program of China (Grant No. 2007CB935701)
文摘We label-free and real-time detected three interaction processes of antigen-antibodies,Human Immunoglobulin G(IgG),Rabbit IgG,and Mouse IgG as the targets,and Goat Anti-human IgG,Goat Anti-rabbit IgG,and Goat Anti-mouse IgG as the probe,by the Oblique-incidence Reflectivity Difference(OIRD) method.The interaction dynamic curves of the OIRD signal,corresponding to the interaction processes of antigen-antibodies,are generated.The reaction times from beginning to equilibrium state are about 1800,900,and 1200 s for Human IgG,Rabbit IgG,and Mouse IgG,respectively.The experimental results demonstrate that the OIRD method not only can distinguish biomolecular interactions,but also can be used in real-time detection of interactions and dynamic processes of biomolecules.
基金supported by the National High Technology Research and Development Program of China ("863" Project) (Grant No.2012AA062601)the National Natural Science Foundation of Major International Cooperation Projects (Grant No. 51010008)
文摘Underground contamination by non-aqueous phase liquids (NAPLs) becomes increasingly serious. Rapid and reliable detection of contaminated zone and degree is the first step to site remediation. In this paper, diesel and fine sand are used as experiment materials to investigate the applicability of using time-domain reflectometry (TDR) to detect LNAPLs contamination. The major work includes: measurement of dielectric constant and electrical conductivity for the diesel-water-air-sand mixtures; measurement of reflection waveform and dielectric constant for specimens with a diesel contaminated layer being sandwiched in sand. The experimental results show the followings: A significant decrease in both dielectric constant and electrical conductivity is observed for the diesel-water-air-sand mixtures when diesel displaces the pore water, and the content of diesel can be calculated by the model; insignificant change in dielectric properties is measured when diesel only displaces the pore gas; when the diesel contaminated sand is sandwiched between two saturated sand layers, the interfaces of the diesel contaminated layer can be identified by analyzing the reflection waveform; for field application, TDR method is valid for the case that LNAPLs seep into saturated sand layer, and the applicability of TDR method in vadose zone depends on the initial saturation of the sand layer. The findings obtained in this paper provide a guidance for the use of TDR for the field investigation of NAPLs contaminated site.
基金supported by the National Basic Research Program of China(Grant No.2007CB35710)
文摘Selected Mouse IgG of 1 mg/mL as target was fabricated on microarray for 500 sample dots.Label-free and real-time reaction dynamic processes were detected between the microarrays with Goat Anti-mouse IgG of 0.02 mg/mL using the obliqueincidence reflectivity difference(OIRD)method.We obtained the reaction results and the reaction dynamic curves of 500 protein dots.In addition,we also used label-free detection of protein microarrays of 10080 sample dots,including BSA and different concentrations of Mouse IgG and Rabbit IgG,by OIRD.The obtained reaction results between the protein microarray with 1 mg/mL Goat Anti-mouse IgG and 1 mg/mL Goat Anti-rabbit IgG are reported herein.Experimental results show that OIRD can be not only label-free high-throughput detection method for biological microarrays but also label-free real-time detection in the interaction processes of biomolecules.