目的探讨尿激酶溶解血凝块的最佳用药剂量、用药时机及药物最佳作用时间，为临床一次性清除血肿提供实验依据。方法采用2个（4×4）拉丁方设计，分析UK剂量[（1万U／ml、2万U／ml、3万U／ml、4万U／ml）]、用药时机（抽血后2、4、8...目的探讨尿激酶溶解血凝块的最佳用药剂量、用药时机及药物最佳作用时间，为临床一次性清除血肿提供实验依据。方法采用2个（4×4）拉丁方设计，分析UK剂量[（1万U／ml、2万U／ml、3万U／ml、4万U／ml）]、用药时机（抽血后2、4、8、16h）和药物作用时间（加UK后2、4、8、16h）对溶凝效果的影响。制备家兔脑内血肿模型，对比观察不同剂量尿激酶（10000U vs 30000U）及等量生理盐水对脑组织形态学的影响。结果UK剂量对溶凝体积的差异有统计学意义（F=9、58，P=0．0105），尿激酶10000U组的溶凝体积明显低于20000U组、30000U组或40000U组，而后3组间的差异无统计学意义。用药时机和药物作用时间对溶凝体积的差异均无统计学意义（F=1．83，P=0．2423；F=2．39，P=0．1673）。光学显微镜和电子显微镜结果显示，3组标本均出现脑出血后急性期的形态学改变，未见其他的细胞形态和超微结构方面的异常改变。结论UK剂量是影响溶凝效果的关键因素，20000～30000U／ml的UK为最佳安全剂量。延迟16h用药不影响溶凝效果，UK作用2h足以发挥溶凝效用。展开更多
The roles of Zn content and thermo-mechanical treatment in affecting microstructures and mechanical properties of Mg-x%Zn-1%Mn（mass fraction,x=4,5,6,7,8,9） wrought Mg alloys were investigated.The microstructure was ...The roles of Zn content and thermo-mechanical treatment in affecting microstructures and mechanical properties of Mg-x%Zn-1%Mn（mass fraction,x=4,5,6,7,8,9） wrought Mg alloys were investigated.The microstructure was extremely refined by dynamic recrystallization（DRC） during extrusion.With increasing Zn content,the DRC grains tended to grow up,at the same time,more second phase streamlines would be present,which restricted the further growing.During solution treatment,the DRC grains would rapidly grow up;however,higher Zn content could hinder the grain boundary expanding,which results in finer ultimate grains.MgZn2 dispersoid particles which are coherent with the matrix would precipitate from the supersaturated solid solution during the one-step aging process,and nano-sized GP zones formed during the pre-aging stage of the two-step aging provide a huge amount of effective nuclei for the MgZn2 phases formed in the second stage,which makes the MgZn2 particles much finer and more dispersed.The mechanical properties of as-extruded samples were not so sensitive to the variation of Zn content,the tensile strength fluctuates between 300 and 320 MPa,and the elongation maintains a high value between 11% and 14%.The strength of aged samples rises as a parabolic curve with increasing Zn content,specifically,the tensile strength of one-step aged samples rises from 278 to 374 MPa,and that of two-step aged ones rises from 284 to 378 MPa,yet the elongation of all aged samples is below 8%.When Zn content exceeds its solid solution limit in Mg-Zn system（6.2%,mass fraction）,the strength rises slowly but the elongation deteriorates sharply,so a Mg-Zn-Mn alloy with 6% Zn possesses the best mechanical properties,that is,the tensile strengths after one-and two-step aging are 352 and 366 MPa,respectively,and the corresponding elongations are 7.98% and 5.2%,respectively.展开更多
目的探讨重组人促红细胞生成素(rh EPO)对人肝癌细胞株(human hepatoma cell line,Hep G2)裸鼠皮下移植瘤的生长影响,及其与促红细胞生成素(EPO)、促红细胞生成素受体(EPOR)、内皮生长因子(VEGF)、Bcl-2(B淋巴细胞/白血病-2基因)表达的...目的探讨重组人促红细胞生成素(rh EPO)对人肝癌细胞株(human hepatoma cell line,Hep G2)裸鼠皮下移植瘤的生长影响,及其与促红细胞生成素(EPO)、促红细胞生成素受体(EPOR)、内皮生长因子(VEGF)、Bcl-2(B淋巴细胞/白血病-2基因)表达的时效和量效关系。方法在BALB/C裸鼠皮下接种人肝癌细胞(Hep G2)建立模型,成瘤后随机将其分为rh EPO低剂量组、高剂量组,阴性对照组(PBS组),分组给药,分别于第2周和第4周观察各组肿瘤生长情况,采用HE染色观察肿瘤组织病理情况,应用real-time PCR技术检测肿瘤组织中EPO、EPOR、VEGF、Bcl-2的m RNA水平。结果对照组的肿瘤体积增长幅度均高于实验组肿瘤体积增长幅度;real-time PCR的结果显示:1与对照组比较,第2周高剂量组和低剂量组EPO m RNA表达降低,差异无统计学意义(P>0.05);EPOR m RNA表达降低(P<0.05);VEGF、Bcl-2 m RNA表达均略升高,但差异无统计学意义(P>0.05)。第4周高剂量组和低剂量组EPO、EPOR m RNA较对照组表达降低(P<0.05);EPO高剂量组的VEGF、Bcl-2 m RNA较对照组表达降低(P<0.05),EPO低剂量组的VEGF、Bcl-2 m RNA较对照组表达降低,但差异无统计学意义(P>0.05)。2第2周高剂量组4个基因表达均较低剂量组降低,但差异无统计学意义(P>0.05)。第4周高剂量组EPO、EPOR m RNA表达较低剂量组略有升高,但差异无统计学意义(P>0.05);高剂量组VEGF、Bcl-2 m RNA表达较低剂量组降低,但差异无统计学意义(P>0.05)。3EPO高剂量组第4周与第2周比较4个基因的表达均有所下降,但差异无统计学意义(P>0.05);EPO低剂量组第4周与第2周比较EPO m RNA表达下降(P<0.05),EPOR、VEGF、Bcl-2m RNA表达有所下降,但差异无统计学意义(P>0.05)。结论不同剂量的rh EPO及rh EPO作用时间的不同对Hep G2肿瘤组织的VEGF、Bcl-2 m RNA表达均无明显影响,无直接证据显示rh EPO可引发Hep G2皮下移植瘤的生长。展开更多
The advantages of OPV （organic photovoltaic） are low cost, little pollution and flexible. But challenge for OPV manufacture still is lacking of accurately performance measurement due to capacitance issue. Firstly, c...The advantages of OPV （organic photovoltaic） are low cost, little pollution and flexible. But challenge for OPV manufacture still is lacking of accurately performance measurement due to capacitance issue. Firstly, characterization of OPV requires considering the slowly temporal response due to capacitance effect, and the relative I-V （current-voltage） curves are strongly dependent on the voltage sweep direction, even for the sweep time only in few seconds or less. Secondly, the IPCE （incident photon-to-electron conversion efficiency） also shows the slowly temporal response due to capacitance effect and is dependent on the wavelength of the incident light. Furthermore, the related features for measuring I-V curves are more sensitive with temperature due to non-linear characteristics issue, but current IPCE spectra of OPV are similar to that happened in conventional crystalline Si or amorphous silicon devices. In this work, we developed a RTOSM （real-time one-sweep method） applied both in I-V and IPCE to analysis different electronic transport materials, and result showed this new approach proposed a good way to slow down testing time and having better accuracy for OPV measurement by eliminating acceptance effect instantly.展开更多
文摘目的探讨尿激酶溶解血凝块的最佳用药剂量、用药时机及药物最佳作用时间，为临床一次性清除血肿提供实验依据。方法采用2个（4×4）拉丁方设计，分析UK剂量[（1万U／ml、2万U／ml、3万U／ml、4万U／ml）]、用药时机（抽血后2、4、8、16h）和药物作用时间（加UK后2、4、8、16h）对溶凝效果的影响。制备家兔脑内血肿模型，对比观察不同剂量尿激酶（10000U vs 30000U）及等量生理盐水对脑组织形态学的影响。结果UK剂量对溶凝体积的差异有统计学意义（F=9、58，P=0．0105），尿激酶10000U组的溶凝体积明显低于20000U组、30000U组或40000U组，而后3组间的差异无统计学意义。用药时机和药物作用时间对溶凝体积的差异均无统计学意义（F=1．83，P=0．2423；F=2．39，P=0．1673）。光学显微镜和电子显微镜结果显示，3组标本均出现脑出血后急性期的形态学改变，未见其他的细胞形态和超微结构方面的异常改变。结论UK剂量是影响溶凝效果的关键因素，20000～30000U／ml的UK为最佳安全剂量。延迟16h用药不影响溶凝效果，UK作用2h足以发挥溶凝效用。
基金Project(2007CB613700)supported by the National Basic Research Program of ChinaProject(2007BAG06B04)supported by the National Science and Technology Pillar Program During the 11th Five-Year Plan Period,China+1 种基金Project(50725413)supported by the National Natural Science Foundation of ChinaProject(CDJXS10132202)supported by the Fundamental Research Funds for the Central Universities,China
文摘The roles of Zn content and thermo-mechanical treatment in affecting microstructures and mechanical properties of Mg-x%Zn-1%Mn（mass fraction,x=4,5,6,7,8,9） wrought Mg alloys were investigated.The microstructure was extremely refined by dynamic recrystallization（DRC） during extrusion.With increasing Zn content,the DRC grains tended to grow up,at the same time,more second phase streamlines would be present,which restricted the further growing.During solution treatment,the DRC grains would rapidly grow up;however,higher Zn content could hinder the grain boundary expanding,which results in finer ultimate grains.MgZn2 dispersoid particles which are coherent with the matrix would precipitate from the supersaturated solid solution during the one-step aging process,and nano-sized GP zones formed during the pre-aging stage of the two-step aging provide a huge amount of effective nuclei for the MgZn2 phases formed in the second stage,which makes the MgZn2 particles much finer and more dispersed.The mechanical properties of as-extruded samples were not so sensitive to the variation of Zn content,the tensile strength fluctuates between 300 and 320 MPa,and the elongation maintains a high value between 11% and 14%.The strength of aged samples rises as a parabolic curve with increasing Zn content,specifically,the tensile strength of one-step aged samples rises from 278 to 374 MPa,and that of two-step aged ones rises from 284 to 378 MPa,yet the elongation of all aged samples is below 8%.When Zn content exceeds its solid solution limit in Mg-Zn system（6.2%,mass fraction）,the strength rises slowly but the elongation deteriorates sharply,so a Mg-Zn-Mn alloy with 6% Zn possesses the best mechanical properties,that is,the tensile strengths after one-and two-step aging are 352 and 366 MPa,respectively,and the corresponding elongations are 7.98% and 5.2%,respectively.
文摘目的探讨重组人促红细胞生成素(rh EPO)对人肝癌细胞株(human hepatoma cell line,Hep G2)裸鼠皮下移植瘤的生长影响,及其与促红细胞生成素(EPO)、促红细胞生成素受体(EPOR)、内皮生长因子(VEGF)、Bcl-2(B淋巴细胞/白血病-2基因)表达的时效和量效关系。方法在BALB/C裸鼠皮下接种人肝癌细胞(Hep G2)建立模型,成瘤后随机将其分为rh EPO低剂量组、高剂量组,阴性对照组(PBS组),分组给药,分别于第2周和第4周观察各组肿瘤生长情况,采用HE染色观察肿瘤组织病理情况,应用real-time PCR技术检测肿瘤组织中EPO、EPOR、VEGF、Bcl-2的m RNA水平。结果对照组的肿瘤体积增长幅度均高于实验组肿瘤体积增长幅度;real-time PCR的结果显示:1与对照组比较,第2周高剂量组和低剂量组EPO m RNA表达降低,差异无统计学意义(P>0.05);EPOR m RNA表达降低(P<0.05);VEGF、Bcl-2 m RNA表达均略升高,但差异无统计学意义(P>0.05)。第4周高剂量组和低剂量组EPO、EPOR m RNA较对照组表达降低(P<0.05);EPO高剂量组的VEGF、Bcl-2 m RNA较对照组表达降低(P<0.05),EPO低剂量组的VEGF、Bcl-2 m RNA较对照组表达降低,但差异无统计学意义(P>0.05)。2第2周高剂量组4个基因表达均较低剂量组降低,但差异无统计学意义(P>0.05)。第4周高剂量组EPO、EPOR m RNA表达较低剂量组略有升高,但差异无统计学意义(P>0.05);高剂量组VEGF、Bcl-2 m RNA表达较低剂量组降低,但差异无统计学意义(P>0.05)。3EPO高剂量组第4周与第2周比较4个基因的表达均有所下降,但差异无统计学意义(P>0.05);EPO低剂量组第4周与第2周比较EPO m RNA表达下降(P<0.05),EPOR、VEGF、Bcl-2m RNA表达有所下降,但差异无统计学意义(P>0.05)。结论不同剂量的rh EPO及rh EPO作用时间的不同对Hep G2肿瘤组织的VEGF、Bcl-2 m RNA表达均无明显影响,无直接证据显示rh EPO可引发Hep G2皮下移植瘤的生长。
文摘The advantages of OPV （organic photovoltaic） are low cost, little pollution and flexible. But challenge for OPV manufacture still is lacking of accurately performance measurement due to capacitance issue. Firstly, characterization of OPV requires considering the slowly temporal response due to capacitance effect, and the relative I-V （current-voltage） curves are strongly dependent on the voltage sweep direction, even for the sweep time only in few seconds or less. Secondly, the IPCE （incident photon-to-electron conversion efficiency） also shows the slowly temporal response due to capacitance effect and is dependent on the wavelength of the incident light. Furthermore, the related features for measuring I-V curves are more sensitive with temperature due to non-linear characteristics issue, but current IPCE spectra of OPV are similar to that happened in conventional crystalline Si or amorphous silicon devices. In this work, we developed a RTOSM （real-time one-sweep method） applied both in I-V and IPCE to analysis different electronic transport materials, and result showed this new approach proposed a good way to slow down testing time and having better accuracy for OPV measurement by eliminating acceptance effect instantly.
