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大鼠游泳运动后骨骼肌UCP_3mRNA表达的时相性变化 被引量:6
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作者 何玉秀 张雪琳 姚玉霞 《中国运动医学杂志》 CAS CSCD 北大核心 2005年第3期289-292,共4页
目的:探讨有氧游泳运动对大鼠骨骼肌UCP3mRNA表达水平的影响,以及运动后骨骼肌UCP3mRNA表达的时相性变化规律。方法:SD雄性大鼠4 8只随机分为对照组、游泳结束后即刻组、运动后1小时组、3小时组、6小时组和12小时组。采用RT -PCR法测定... 目的:探讨有氧游泳运动对大鼠骨骼肌UCP3mRNA表达水平的影响,以及运动后骨骼肌UCP3mRNA表达的时相性变化规律。方法:SD雄性大鼠4 8只随机分为对照组、游泳结束后即刻组、运动后1小时组、3小时组、6小时组和12小时组。采用RT -PCR法测定各组大鼠骨骼肌UCP3mRNA表达水平。结果:6 0分钟有氧游泳运动后大鼠骨骼肌UCP3mRNA表达增加,且运动结束后即刻和恢复期1、3、6和12小时UCP3mRNA表达呈时相性变化,较对照组分别增加15 5 . 8% ,173. 6 % ,2 5 2 . 4 % ,16 5. 8%和12 .9 1%。结论:(1)有氧游泳运动可能通过提高运动时和运动后恢复期大鼠骨骼肌UCP3mRNA的表达,增加能量消耗。(2 )运动后骨骼肌UCP3mRNA过表达的时相性变化可能受多种因素影响。 展开更多
关键词 肥胖 解偶联蛋白 游泳运动 运动后恢复期 mRNA表达 SD雄性大鼠 时相性变化 UCP3 骨骼肌 RT-PCR法
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大鼠游泳运动后骨骼肌PGC-1α mRNA表达的时相性变化 被引量:4
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作者 赵婷婷 丁树哲 《上海体育学院学报》 CSSCI 北大核心 2006年第3期41-44,共4页
采用实验法,检测一次长时游泳运动对大鼠骨骼肌PGC-1αmRNA表达水平的影响,以及运动后骨骼肌PGC-1αmRNA表达的时相性变化规律。将SD雄性大鼠随机分为对照组、运动后3 h组、运动后6 h组1、2 h组1、8 h组和24 h组、运动4 d后18 h组。采用... 采用实验法,检测一次长时游泳运动对大鼠骨骼肌PGC-1αmRNA表达水平的影响,以及运动后骨骼肌PGC-1αmRNA表达的时相性变化规律。将SD雄性大鼠随机分为对照组、运动后3 h组、运动后6 h组1、2 h组1、8 h组和24 h组、运动4 d后18 h组。采用SYBRGreenⅠ逆转录实时荧光定量PCR方法,测定各组大鼠骨骼肌PGC-1αmRNA表达水平。结果:PGC-1α基因在运动后6 h组、运动4 d后18 h组的表达水平高于对照组及其他组。结论:PGC-1αmRNA在游泳运动后表达的改变是有时相性的;游泳运动可以刺激骨骼肌线粒体生物发生相关因子的表达,从而调节线粒体的功能。 展开更多
关键词 大鼠 游泳运动 骨骼肌 PGC-1αmRNA表达 时相性变化 实时荧光定量PCR方法 线粒体
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体外培养的新生大鼠成骨细胞形态及功能的时相性变化 被引量:1
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作者 张克勤 陈家伟 +2 位作者 朱兰兰 周惠英 王美莲 《江苏医药》 CAS CSCD 北大核心 2001年第9期660-662,共3页
目的 探索大鼠成骨细胞 (OB)在体外不同时间的形态及功能变化。方法 在为期 2 8天的细胞培养中动态观察了新生大鼠头盖骨OB的活细胞形态变化 ,并与其皮肤成纤维细胞 (FB)的形态变化作了比较 ,测定了OB中Ⅰ型原胶原羧基端前肽 (PICP)... 目的 探索大鼠成骨细胞 (OB)在体外不同时间的形态及功能变化。方法 在为期 2 8天的细胞培养中动态观察了新生大鼠头盖骨OB的活细胞形态变化 ,并与其皮肤成纤维细胞 (FB)的形态变化作了比较 ,测定了OB中Ⅰ型原胶原羧基端前肽 (PICP)、骨钙素 (BGP)和碱性磷酸酶(ALP)的表达量变化。结果 OB与FB在早期散在分布时形态相似 ,汇合以后两者逐渐出现差异 ;OB在 2 8天才可用VonKossa法染到明显钙盐沉着 ;PICP、BGP分别于初始植入后第 7、19天有表达高峰 ,ALP在 12~ 16天和≥ 2 8天有两个表达高峰。 展开更多
关键词 成骨细胞 大鼠 形态学 功能 体外培养 时相性变化
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大鼠有氧运动力竭过程中内皮素的时相性变化
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作者 朱磊 刘洪珍 《体育科学研究》 2009年第2期64-66,共3页
为了揭示有氧运动力竭过程中大鼠血清内皮素浓度时相性变化的规律,采用文献资料法、实验分析法、数理统计法等研究方法,对有氧运动力竭及恢复过程中大鼠进行了实验研究。研究表明:运动1 h和运动2 h内皮素含量下降;运动3 h、力竭即刻和... 为了揭示有氧运动力竭过程中大鼠血清内皮素浓度时相性变化的规律,采用文献资料法、实验分析法、数理统计法等研究方法,对有氧运动力竭及恢复过程中大鼠进行了实验研究。研究表明:运动1 h和运动2 h内皮素含量下降;运动3 h、力竭即刻和力竭后恢复2 h内皮素表达增加;力竭恢复12 h,内皮素恢复至安静水平。研究认为大鼠有氧运动力竭过程中,血清中ET浓度会随着运动时间而改变,因此,在不同运动时相ET对于心血管调节效应不同。 展开更多
关键词 力竭运动 内皮素 时相性变化
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Airborne Pollen Grains Of Afyon, Turkey 被引量:3
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作者 Adem BICAKCI 1, Süheyla ERGUN 2, Sevcan TATLIDIL 1, Hulusi MALYER 1, Sabri ?ZYURT 2, Ahmet AKKAYA 3, Nihat SAPAN 5 Department of Biology, Faculty of Science, University of Uludag, Bursa, Turkey 2. Department of Biology, Faculty of Science, University of Afyon Kocatepe, Afyon Turkey +1 位作者 3. Department of Pulmonology, Faculty of Medicine, University of Süleyman Demirel, Isparta, Turkey 4. Division of Pediatric Allergy, Faculty of Medicine, University of Uludag, Bursa, Turkey) 《Acta Botanica Sinica》 CSCD 2002年第11期1371-1375,共5页
The airborne pollen grains of Afyon have been studied for a two_year period (1999-2000) with a Durham sampler. A total of 14 367 pollen grains belonging to 40 taxa have been identified and recorded with some unidentif... The airborne pollen grains of Afyon have been studied for a two_year period (1999-2000) with a Durham sampler. A total of 14 367 pollen grains belonging to 40 taxa have been identified and recorded with some unidentified ones. Of them, 6 732 were identified in 1999 and 7 635 in 2000. Of the total pollen grains, 69.67% were arboreal, 26.64% non_arboreal and 3.68 % unidentified. The majority of the investigated pollen grains were from Pinus, Gramineae, Cupressaceae, Platanus , Chenopodiaceae/Amaranthaceae, Quercus, Ailanthus, Moraceae, Juglans , Salix, Cedrus and Rosaceae. The highest level of pollen grains was in May. 展开更多
关键词 TURKEY Afyon pollen calendar pollen grains
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The Project Siberian High in CMIP5 Models
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作者 LI Fei GAO Yong-Qi 《Atmospheric and Oceanic Science Letters》 CSCD 2015年第4期179-184,共6页
The Siberian high(SH)experienced a decline from the 1970s to 1990s and a recovery in recent years.The evolution of the SH under global warming is unclear.In this study,41 Coupled Model Intercomparison Project Phase 5(... The Siberian high(SH)experienced a decline from the 1970s to 1990s and a recovery in recent years.The evolution of the SH under global warming is unclear.In this study,41 Coupled Model Intercomparison Project Phase 5(CMIP5)climate models are evaluated in terms of their ability to simulate the temporal evolution of the SH in the 19th and 20th centuries and the spatial pattern of the SH during 1981–2005.The results show that 12models can capture the temporal evolution of the SH center intensity(SHCI)for 1872–2005.The linear correlation coefficient between the SHCI from the Twentieth Century Reanalysis and the simulated SHCI from the multi-model ensemble(MME)of the 12 models is 0.3 on annual and inter-annual scales(above the 99%confidence level).On decadal and multi-decadal time scales,the MME also captures the pronounced reduction(between 1981–2000and 1881–1900 period)and the recovery(during1991–2005)of the SH intensity.Finally,the future evolution of the SH is investigated using the MME of the 12models under the+4.5 and+8.5 W m-2 Representative Concentration Pathway(RCP)scenarios(RCP4.5 and RCP8.5).It is shown that the SHCI,similar to the SHCI in the 20th century,has no significant long-term trend in the 21st century under global warming(RCP8.5 scenario).At the end of 21st century(2081–2100),the SH shows stronger interannual variability than the SH at the end of20th century(1981–2000).The increased interannual variability likely favors the increased interannual variability in winter air temperature over midlatitude Eurasia at the end of 21st century. 展开更多
关键词 Siberian high global warming CLIMATOLOGY interannual variability CMIP5
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Fragmented condensates of singly trapped dipolar Bose gases
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作者 LU HaiYan YI Su 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2012年第9期1535-1540,共6页
By employing the method of the multiconfigurational time-dependent Hartree for bosons,we investigate the ground state properties of a singly trapped dipolar gas of spinless bosons.We show that the repulsive interactio... By employing the method of the multiconfigurational time-dependent Hartree for bosons,we investigate the ground state properties of a singly trapped dipolar gas of spinless bosons.We show that the repulsive interactions favor the formation of the fragmented ground state.In particular,we find the formation of the fragmented state is mainly due to the interaction energies associated with the one-and two-particle exchanges between orbitals.We also obtain the stability diagram of the system and find that the stability of the system is significantly enhanced by the appearance of the fragmentation. 展开更多
关键词 fragmentation Bose-Einsein condensate dipole-dipole interation
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