Carbon fiber nanoelectrodes(200-300 nm) were firstly used to amperometricaly monitor the dopamine release from single PC12 cells with temporal resolution and especially more higher spatial resolution than those obtain...Carbon fiber nanoelectrodes(200-300 nm) were firstly used to amperometricaly monitor the dopamine release from single PC12 cells with temporal resolution and especially more higher spatial resolution than those obtained by using microelectrodes. When the nanoelectrode was in a distance 1 μm above the PC12 cell, only one peak signal corresponding to a single vesicle exocytotic event was detected caused from the stimulation with 1 mmol/L nicotine. The spatial difference of exocytosis was also detected by placing the electrode onto the different locations of the cell body, the results have demostrated that the spatial distribution of dopamine in cells is not uniform and the time for stimulating secretion is very different. Nanoelectrodes electrochemical method can provide a powerful tool for observing the temporal and spatial characteristics of the secretion from single cells directly.展开更多
The stability of the surrounding rock mass around cross tunnel in the right bank slope of Dagangshan hydropower station, in the southwestern China, was analyzed by microseismic monitoring as well as numerical simulati...The stability of the surrounding rock mass around cross tunnel in the right bank slope of Dagangshan hydropower station, in the southwestern China, was analyzed by microseismic monitoring as well as numerical simulations. The realistic failure process analysis code （abbreviated as RFPA3D） was employed to reproduce the initiation, propagation, coalescence and interactions of micro-fractures, the evolution of associated stress fields and acoustic emission （AE） activities during the whole failure processes of the surrounding rock mass around cross tunnel. Combined with microseismic activities by microseismic monitoring on the fight bank slope, the spatial-temporal evolution and the micro-fracture precursor characteristics during the complete process of progressive failure of the surrounding rock mass around cross tunnel were discussed and the energy release law of the surrounding rock mass around the cross tunnel was obtained. The result shows that the precursor characteristic of microfractures occurring in rock mass is an effective approach to early warn catastrophic damage of rock mass around cross tunnel. Moreover, the heterogeneity of rock mass is the source and internal cause of the failure precursor of rock mass.展开更多
With long-term marine surveys and research,and especially with the development of new marine environment monitoring technologies,prodigious amounts of complex marine environmental data are generated,and continuously i...With long-term marine surveys and research,and especially with the development of new marine environment monitoring technologies,prodigious amounts of complex marine environmental data are generated,and continuously increase rapidly.Features of these data include massive volume,widespread distribution,multiple-sources,heterogeneous,multi-dimensional and dynamic in structure and time.The present study recommends an integrative visualization solution for these data,to enhance the visual display of data and data archives,and to develop a joint use of these data distributed among different organizations or communities.This study also analyses the web services technologies and defines the concept of the marine information gird,then focuses on the spatiotemporal visualization method and proposes a process-oriented spatiotemporal visualization method.We discuss how marine environmental data can be organized based on the spatiotemporal visualization method,and how organized data are represented for use with web services and stored in a reusable fashion.In addition,we provide an original visualization architecture that is integrative and based on the explored technologies.In the end,we propose a prototype system of marine environmental data of the South China Sea for visualizations of Argo floats,sea surface temperature fields,sea current fields,salinity,in-situ investigation data,and ocean stations.An integration visualization architecture is illustrated on the prototype system,which highlights the process-oriented temporal visualization method and demonstrates the benefit of the architecture and the methods described in this study.展开更多
文摘Carbon fiber nanoelectrodes(200-300 nm) were firstly used to amperometricaly monitor the dopamine release from single PC12 cells with temporal resolution and especially more higher spatial resolution than those obtained by using microelectrodes. When the nanoelectrode was in a distance 1 μm above the PC12 cell, only one peak signal corresponding to a single vesicle exocytotic event was detected caused from the stimulation with 1 mmol/L nicotine. The spatial difference of exocytosis was also detected by placing the electrode onto the different locations of the cell body, the results have demostrated that the spatial distribution of dopamine in cells is not uniform and the time for stimulating secretion is very different. Nanoelectrodes electrochemical method can provide a powerful tool for observing the temporal and spatial characteristics of the secretion from single cells directly.
基金Projects(50820125405, 51004020, 51174039, 4112265) supported by the National Natural Science Foundation of ChinaProject(201104563) supported by the China Postdoctoral Science Foundation+3 种基金Project(2011CB013503) supported by the National Basic Research Program of ChinaProject(51274053) supported by the Fundamental Research Funds for the Central Universities of ChinaProject(200960) supported by the Foundation for the Author of National Excellent Doctoral Dissertation of ChinaProject(NECT-09-0258) supported by the New Century Excellent Talents in University of China
文摘The stability of the surrounding rock mass around cross tunnel in the right bank slope of Dagangshan hydropower station, in the southwestern China, was analyzed by microseismic monitoring as well as numerical simulations. The realistic failure process analysis code （abbreviated as RFPA3D） was employed to reproduce the initiation, propagation, coalescence and interactions of micro-fractures, the evolution of associated stress fields and acoustic emission （AE） activities during the whole failure processes of the surrounding rock mass around cross tunnel. Combined with microseismic activities by microseismic monitoring on the fight bank slope, the spatial-temporal evolution and the micro-fracture precursor characteristics during the complete process of progressive failure of the surrounding rock mass around cross tunnel were discussed and the energy release law of the surrounding rock mass around the cross tunnel was obtained. The result shows that the precursor characteristic of microfractures occurring in rock mass is an effective approach to early warn catastrophic damage of rock mass around cross tunnel. Moreover, the heterogeneity of rock mass is the source and internal cause of the failure precursor of rock mass.
基金Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (No.KZCX1-YW-12-04)the National High Technology Research and Development Program of China (863 Program) (Nos.2009AA12Z148,2007AA092202)Support for this study was provided by the Institute of Geographical Sciences and the Natural Resources Research,Chinese Academy of Science (IGSNRR,CAS) and the Institute of Oceanology, CAS
文摘With long-term marine surveys and research,and especially with the development of new marine environment monitoring technologies,prodigious amounts of complex marine environmental data are generated,and continuously increase rapidly.Features of these data include massive volume,widespread distribution,multiple-sources,heterogeneous,multi-dimensional and dynamic in structure and time.The present study recommends an integrative visualization solution for these data,to enhance the visual display of data and data archives,and to develop a joint use of these data distributed among different organizations or communities.This study also analyses the web services technologies and defines the concept of the marine information gird,then focuses on the spatiotemporal visualization method and proposes a process-oriented spatiotemporal visualization method.We discuss how marine environmental data can be organized based on the spatiotemporal visualization method,and how organized data are represented for use with web services and stored in a reusable fashion.In addition,we provide an original visualization architecture that is integrative and based on the explored technologies.In the end,we propose a prototype system of marine environmental data of the South China Sea for visualizations of Argo floats,sea surface temperature fields,sea current fields,salinity,in-situ investigation data,and ocean stations.An integration visualization architecture is illustrated on the prototype system,which highlights the process-oriented temporal visualization method and demonstrates the benefit of the architecture and the methods described in this study.
