This study aims to explore the effect of GA_(3) concentration and soaking time on seed germination characteristics of blueberry.To be specific,big(B),medium-sized(M),and small(S)blueberry seeds were soaked in 0,100,20...This study aims to explore the effect of GA_(3) concentration and soaking time on seed germination characteristics of blueberry.To be specific,big(B),medium-sized(M),and small(S)blueberry seeds were soaked in 0,100,200,and 300 mg/L GA_(3) solution for 18 h and 200 mg/L GA_(3) solution for 0,24,and 48 h,respectively.The results showed that 300 mg/L GA_(3) had significant effect on the germination of B and M seeds(P<0.01)and enabled the germination 4~5 d and 7~8 d earlier,respectively.Soaking with 200 mg/L GA_(3) for 24 h was conducive to the germination of B and M seeds(P<0.05).However,GA_(3) concentration and soaking time had no obviously promoting effect on the germination of S seeds.The interactions between GA_(3) concentration and seed size and between soaking time and seed size were significant(P<0.01).展开更多
In order to solve the problem of low emergence rate of Bupleurum chinense seeds, and screen out the best germination conditions, the seeds of Bupleurum chinense L. were treated with different soaking time, germinatio...In order to solve the problem of low emergence rate of Bupleurum chinense seeds, and screen out the best germination conditions, the seeds of Bupleurum chinense L. were treated with different soaking time, germination temperature and exogenous regulating substances to improve the germination rate. The results showed that the germination rate of fresh water at 12 h was significantly higher than that at 8 h and 36 h; The germination rate of seeds treated with constant temperature 15℃ and variable temperature 25 ℃/15 ℃ (day/night) was significantly higher than that of other temperature treatments. Seed soaking time and culture temperature also influenced seed germination start day and peak day. Different concentrations of regulatory substances could promote seeds germination. The germination rate of seeds induced by 0.50 mg/L 6-BA was the highest, the start day and peak day were 4 days and 5 days earlier than control check (CK) respectively.展开更多
In order to simultaneously measure the initiation toughness of pure mode Ⅰ and mode Ⅱ cracks in one specimen,a large-size double-cracked concave-convex plate(DCCP)specimen configuration was proposed.Impacting tests ...In order to simultaneously measure the initiation toughness of pure mode Ⅰ and mode Ⅱ cracks in one specimen,a large-size double-cracked concave-convex plate(DCCP)specimen configuration was proposed.Impacting tests were implemented in the drop plate impact device.Strain gauges were employed to measure impact loads and crack initiation time.The corresponding numerical model was established by using the dynamic finite difference program AUTODYN,and the experimental-numerical method and ABAQUS code were utilized to obtain the initial fracture toughness of the crack.Using experiments and numerical research,we concluded that the DCCP specimen is suitable for measuring the initial fracture toughness of pure mode Ⅰ and mode Ⅱ cracks at the same time;the dynamic initiation toughness increases with the increase of loading rate and the crack initiation time decreases with increasing loading rate;the initiation toughness of mode Ⅱ crack is 0.5 times that of mode Ⅰ crack when subjected to the same loading rate.For the pre-crack in the vicinity of the bottom of a sample,when its length increases from 20 to 100 mm,the dynamic initiation toughness of the pure mode Ⅰ crack gradually decreases,and the longer the lower crack length is,the easier the crack would initiate,but the dynamic initiation toughness of pure mode Ⅱ crack varies little.展开更多
Ustilaginoidea virens is the causal agent of a serious disease of rice. To reveal the relationship between germination and the 3'-5'-cyclic adenosine monophosphate(c AMP) content from the dormant(black or gree...Ustilaginoidea virens is the causal agent of a serious disease of rice. To reveal the relationship between germination and the 3'-5'-cyclic adenosine monophosphate(c AMP) content from the dormant(black or green-black) and non-dormant(yellow)chlamydospore in Ustiloginoidea virens,this study adopts ultrasonic-bath method and high-performance liquid chromatography(HPLC) method, for extraction c AMP content of the different color chlamydospore. The results demonstrated that, as for the freshly chlamydospores collected from the false smut balls during their germination, c AMP content of yellow chlamydospore appeared a slight growth during 0-12 h, and showed a rapid declining as the germination time extended(12-48 h). Above all, in yellow chlamydospores, the germination rate and the content of c AMP presented a very notably negative correlation(|r|=0.861 9>r0.01=0.834), but the correlation between germination rate and content of c AMP was not obvious in black chlamydospores. The germination rate and the content of c AMP presented a notably positive correlation under different storage period(0, 2, 4, 6, 8,10 months) of the two color chlamydospores(the yellow of chlamydospore: |r | = 0.785 1 > r0.05= 0.707; the black of chlamydospore: |r| = 0.957 9 > r0.05= 0.707).展开更多
The effect of NaCI and proline on somatic embryos formation and germination of date palm (Phoenix dactylifera L.) cv. Barhee was studied. Quarters of shoot tips were cultured on MS solid growth media supplied with d...The effect of NaCI and proline on somatic embryos formation and germination of date palm (Phoenix dactylifera L.) cv. Barhee was studied. Quarters of shoot tips were cultured on MS solid growth media supplied with different concentrations of salt (NaCI) (0.5%, 1%, 1.5%, 2%, 2.5% and 3%, respectively) and proline (25, 50 and 75 mg L1) either individually or in combination. Results showed that the addition of NaC1 (starting at 1%) caused a significant increase in the cylindrical embryos formation time, while their lengths and fresh weights were decreased at salt concentrations (0.5% and 2%). No significant effect was noticed on the formation time by using proline except at concentration of 25 mg L^-1 which caused an increase in their lengths and fresh weights. The interaction between all salt concentrations and proline (25 mg LL) caused a significant increase in the formation time as well as in their lengths and fresh weights. The addition of NaC1 to the media caused a significant effect on the germination time and a reduction on the percentages of somatic embryo germination starting at 2% and the following ones. A reduction in the lengths of radicals and the plumules of the formed embryos was noticed at 1.5% NaCI. Proline addition reduced the initial time and increased the germination percentages as well as the lengths of radicals and plumules. The interaction between salt and proline treatments caused a significant reduction in the initiation time and an increase in the germination percentages and lengths of radicals and plumules.展开更多
Aims Germination is the earliest life-history transition of a plant species.It determines the ecological breadth and geographic ranges of a species and has major effects on its invasion potential.The largest spread of...Aims Germination is the earliest life-history transition of a plant species.It determines the ecological breadth and geographic ranges of a species and has major effects on its invasion potential.The largest spread of the invasive salt-marsh cordgrass Spartina alterniflora in China,where it extends to latitudes lower than its native range in North America,provides an opportunity to examine germination trait variation across latitudes within and among its invasive and native ranges.Methods We studied seed germination traits of S.alterniflora using seeds collected from 10 locations across latitudes in its invasive range(China,20°–40°N)and 16 locations across latitudes in its native range(USA,27°–43°N)in growth chambers with 0 PSU sterilized distilled water.We further evaluated how climate and tide range in the original locations influenced germination traits.Important Findings Native populations showed higher(~10%)germination percentage and significantly higher(~20%)germination index than invasive populations did,but invasive populations germinated significantly earlier(~3 days)than native populations.Germination percentage and germination index increased with latitude in the invasive range but decreased with latitude in the native range.The mean germination time decreased with latitude in the invasive range and paralleled that in the native range.Germination percentage and germination index were negatively correlated with mean daily temperature(Tmean),mean daily maximum temperature(Tmax)and mean daily minimum temperature(Tmin),and inversely correlated with Tmean,Tmax and Tmin in the native range.However,the mean germination time was positively correlated with Tmean,Tmax and Tmean in both ranges.Our results demonstrate that invasive and native populations have evolved different latitudinal clines in germination percentage and index,but the mean germination time of the invasive population mirrored the latitudinal cline observed in the native range,suggesting that germination strategy across latitudes may change during invasion process.展开更多
文摘This study aims to explore the effect of GA_(3) concentration and soaking time on seed germination characteristics of blueberry.To be specific,big(B),medium-sized(M),and small(S)blueberry seeds were soaked in 0,100,200,and 300 mg/L GA_(3) solution for 18 h and 200 mg/L GA_(3) solution for 0,24,and 48 h,respectively.The results showed that 300 mg/L GA_(3) had significant effect on the germination of B and M seeds(P<0.01)and enabled the germination 4~5 d and 7~8 d earlier,respectively.Soaking with 200 mg/L GA_(3) for 24 h was conducive to the germination of B and M seeds(P<0.05).However,GA_(3) concentration and soaking time had no obviously promoting effect on the germination of S seeds.The interactions between GA_(3) concentration and seed size and between soaking time and seed size were significant(P<0.01).
文摘In order to solve the problem of low emergence rate of Bupleurum chinense seeds, and screen out the best germination conditions, the seeds of Bupleurum chinense L. were treated with different soaking time, germination temperature and exogenous regulating substances to improve the germination rate. The results showed that the germination rate of fresh water at 12 h was significantly higher than that at 8 h and 36 h; The germination rate of seeds treated with constant temperature 15℃ and variable temperature 25 ℃/15 ℃ (day/night) was significantly higher than that of other temperature treatments. Seed soaking time and culture temperature also influenced seed germination start day and peak day. Different concentrations of regulatory substances could promote seeds germination. The germination rate of seeds induced by 0.50 mg/L 6-BA was the highest, the start day and peak day were 4 days and 5 days earlier than control check (CK) respectively.
基金Projects(U19A2098,1210021843)supported by the National Natural Science Foundation of ChinaProject(2021SCU12130)supported by Fundamental Research Funds for the Central Universities,China+1 种基金Project(2021YJ0511)supported by the Sichuan Science and Technology Program,ChinaProjects(DESEYU202205,DESE202005)supported by the Open Fund of Key Laboratory of Deep Earth Science and Engineering,China。
文摘In order to simultaneously measure the initiation toughness of pure mode Ⅰ and mode Ⅱ cracks in one specimen,a large-size double-cracked concave-convex plate(DCCP)specimen configuration was proposed.Impacting tests were implemented in the drop plate impact device.Strain gauges were employed to measure impact loads and crack initiation time.The corresponding numerical model was established by using the dynamic finite difference program AUTODYN,and the experimental-numerical method and ABAQUS code were utilized to obtain the initial fracture toughness of the crack.Using experiments and numerical research,we concluded that the DCCP specimen is suitable for measuring the initial fracture toughness of pure mode Ⅰ and mode Ⅱ cracks at the same time;the dynamic initiation toughness increases with the increase of loading rate and the crack initiation time decreases with increasing loading rate;the initiation toughness of mode Ⅱ crack is 0.5 times that of mode Ⅰ crack when subjected to the same loading rate.For the pre-crack in the vicinity of the bottom of a sample,when its length increases from 20 to 100 mm,the dynamic initiation toughness of the pure mode Ⅰ crack gradually decreases,and the longer the lower crack length is,the easier the crack would initiate,but the dynamic initiation toughness of pure mode Ⅱ crack varies little.
基金supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China(Class tutors,Grant No.20114320110008)the Scientific Research Fund of Hunan Provincial Education Department,China(Grant No.11A052,No.12JJ4028)Inquiry Learning and Innovative Experiment Projects of Hunan Provincial Undergraduate(SCX1219)
文摘Ustilaginoidea virens is the causal agent of a serious disease of rice. To reveal the relationship between germination and the 3'-5'-cyclic adenosine monophosphate(c AMP) content from the dormant(black or green-black) and non-dormant(yellow)chlamydospore in Ustiloginoidea virens,this study adopts ultrasonic-bath method and high-performance liquid chromatography(HPLC) method, for extraction c AMP content of the different color chlamydospore. The results demonstrated that, as for the freshly chlamydospores collected from the false smut balls during their germination, c AMP content of yellow chlamydospore appeared a slight growth during 0-12 h, and showed a rapid declining as the germination time extended(12-48 h). Above all, in yellow chlamydospores, the germination rate and the content of c AMP presented a very notably negative correlation(|r|=0.861 9>r0.01=0.834), but the correlation between germination rate and content of c AMP was not obvious in black chlamydospores. The germination rate and the content of c AMP presented a notably positive correlation under different storage period(0, 2, 4, 6, 8,10 months) of the two color chlamydospores(the yellow of chlamydospore: |r | = 0.785 1 > r0.05= 0.707; the black of chlamydospore: |r| = 0.957 9 > r0.05= 0.707).
文摘The effect of NaCI and proline on somatic embryos formation and germination of date palm (Phoenix dactylifera L.) cv. Barhee was studied. Quarters of shoot tips were cultured on MS solid growth media supplied with different concentrations of salt (NaCI) (0.5%, 1%, 1.5%, 2%, 2.5% and 3%, respectively) and proline (25, 50 and 75 mg L1) either individually or in combination. Results showed that the addition of NaC1 (starting at 1%) caused a significant increase in the cylindrical embryos formation time, while their lengths and fresh weights were decreased at salt concentrations (0.5% and 2%). No significant effect was noticed on the formation time by using proline except at concentration of 25 mg L^-1 which caused an increase in their lengths and fresh weights. The interaction between all salt concentrations and proline (25 mg LL) caused a significant increase in the formation time as well as in their lengths and fresh weights. The addition of NaC1 to the media caused a significant effect on the germination time and a reduction on the percentages of somatic embryo germination starting at 2% and the following ones. A reduction in the lengths of radicals and the plumules of the formed embryos was noticed at 1.5% NaCI. Proline addition reduced the initial time and increased the germination percentages as well as the lengths of radicals and plumules. The interaction between salt and proline treatments caused a significant reduction in the initiation time and an increase in the germination percentages and lengths of radicals and plumules.
基金the National Natural Science Foundation of China(31770464,31470485,41276078,32001234)China Postdoctoral Science Foundation(2019M662239,2020T130363).
文摘Aims Germination is the earliest life-history transition of a plant species.It determines the ecological breadth and geographic ranges of a species and has major effects on its invasion potential.The largest spread of the invasive salt-marsh cordgrass Spartina alterniflora in China,where it extends to latitudes lower than its native range in North America,provides an opportunity to examine germination trait variation across latitudes within and among its invasive and native ranges.Methods We studied seed germination traits of S.alterniflora using seeds collected from 10 locations across latitudes in its invasive range(China,20°–40°N)and 16 locations across latitudes in its native range(USA,27°–43°N)in growth chambers with 0 PSU sterilized distilled water.We further evaluated how climate and tide range in the original locations influenced germination traits.Important Findings Native populations showed higher(~10%)germination percentage and significantly higher(~20%)germination index than invasive populations did,but invasive populations germinated significantly earlier(~3 days)than native populations.Germination percentage and germination index increased with latitude in the invasive range but decreased with latitude in the native range.The mean germination time decreased with latitude in the invasive range and paralleled that in the native range.Germination percentage and germination index were negatively correlated with mean daily temperature(Tmean),mean daily maximum temperature(Tmax)and mean daily minimum temperature(Tmin),and inversely correlated with Tmean,Tmax and Tmin in the native range.However,the mean germination time was positively correlated with Tmean,Tmax and Tmean in both ranges.Our results demonstrate that invasive and native populations have evolved different latitudinal clines in germination percentage and index,but the mean germination time of the invasive population mirrored the latitudinal cline observed in the native range,suggesting that germination strategy across latitudes may change during invasion process.