An improved in-plane insensitive double-aperture digital speckle shearing interferometric technique is proposed to measure the first derivative of out-of-plane displacement （slope）. The temporal phase-shifting metho...An improved in-plane insensitive double-aperture digital speckle shearing interferometric technique is proposed to measure the first derivative of out-of-plane displacement （slope）. The temporal phase-shifting method is used for the quantitative analysis of fringes. The designed system employs a double-aperture arrange- ment placed in front of the imaging lens. A glass wedge covers one of the two apertures to introduce a laterally shear. The experimental specimen is a circular aluminum plate, clamped along its edge and subjected to both out- of-plane deflection and in-plane rotation. Experimental results show that the fringes obtained from the proposed optical configuration represent pure slope contour distributions, and that the contributions from the in-plane dis- placement components are completely eliminated. Theoretical and experimental results are in good agreement.展开更多
OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell ...OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell linesand used SELDI-TOF-MS to detect the differential expressionof the proteins in the 2 cell lines.The proteomic spectra weregenerated using weak cation exchange chips.The biomarkerswere validated by analyzing serum proteins or peptides in ovariancancer patients,relapsed ovarian cancer patients,patients withbenign ovarian tumors,and healthy people.RESULTS Four proteins in the culture supernatant fromHO-8910PM cells were up-regulated,relative to the culturesupernatant of HO-8910 cells.One protein (3,144 Da m/z value)was up-regulated in both the cell lysis solution and in the culturesupernatant of HO-8910PM cells.In addition,expression of the3,144 Da m/z protein differed significantly between serum fromthe 26 ovarian cancer patients,from the 22 relapsed ovarianpatients and from the 37 healthy women (P<0.01).However,therewas no difference between patients with benign ovarian tumorsand healthy people (P>0.5).CONCLUSION Ovarian cancer cell lines with high or lowmetastatic potential have distinct protein profiles.Protein 3,144Da m/z could be a useful biomarker for diagnosing ovarian cancermetastasis.展开更多
Spiking deconvolution is a standard Wiener Levinson algorithm. The autocorrelation of the design time gate is computed and there is a specified taper on the design gate before the autoeorrelation is done. The standard...Spiking deconvolution is a standard Wiener Levinson algorithm. The autocorrelation of the design time gate is computed and there is a specified taper on the design gate before the autoeorrelation is done. The standard equations are set up, prewhitening is added to the zero lag value of the autocorrelation and the matrix is inverted to derive the spiking operator. In this study, the authors describe a technique for performing spiking deconvolution on prestack time migration （PSTM） data, to test the effect of operator length and percent prewhitening in spiking deconvolution and apply spiking deconvolution trace by trace, with operator lengths 15ms, 10 ms and 5 ms when percent prewhitening 0% , 40ms and 60ms for percent prewhitening 1%. The results show when prewhitening is 0% the shorter operator gives better results, but when value of prewhitening is bigger than 0% it is better to use longer operator lengths.展开更多
Land plants in natural soil form intimate relationships with the diverse root bacterial microbiota. A growing body of evidence shows that these microbes are important for plant growth and health. Root microbiota compo...Land plants in natural soil form intimate relationships with the diverse root bacterial microbiota. A growing body of evidence shows that these microbes are important for plant growth and health. Root microbiota composition has been widely studied in several model plants and crops; however, little is known about how root microbiota vary throughout the plant's life cycle under field conditions. We performed longitudinal dense sampling in field trials to track the time-series shift of the root microbiota from two representative rice cultivars in two separate locations in China. We found that the rice root microbiota varied dramatically during the vegetative stages and stabilized from the beginning of the reproductive stage, after which the root microbiota underwent relatively minor changes until rice ripening. Notably, both rice genotype and geographical location influenced the patterns of root microbiota shift that occurred during plant growth. The relative abundance of Deltaproteobacteria in roots significantly increased overtime throughout the entire life cycle of rice, while that of Betaproteobacteria, Firmicutes, and Gammaproteobacteria decreased. By a machine learning approach, we identified biomarker taxa and established a model to correlate root microbiota with rice resident time in the field(e.g., Nitrospira accumulated from 5 weeks/tillering in field-grown rice). Our work provides insights into the process of rice root microbiota establishment.展开更多
We demonstrate a photon counting laser ranging experiment with a four-channel single-photon detector(SPD). The multi-channel SPD improve the counting rate more than 4×10~7 cps, which makes possible for the distan...We demonstrate a photon counting laser ranging experiment with a four-channel single-photon detector(SPD). The multi-channel SPD improve the counting rate more than 4×10~7 cps, which makes possible for the distance measurement performed even in daylight. However, the time-correlated single-photon counting(TCSPC) technique cannot distill the signal easily while the fast moving targets are submersed in the strong background. We propose a dynamic TCSPC method for fast moving targets measurement by varying coincidence window in real time. In the experiment, we prove that targets with velocity of 5 km/s can be detected according to the method, while the echo rate is 20% with the background counts of more than 1.2×10~7 cps.展开更多
We investigate the effect of phase shift on the perfect state transfer through two parallel one-dimensional ring-shaped spin chains. We find that the total success probability can be significantly enhanced by phase sh...We investigate the effect of phase shift on the perfect state transfer through two parallel one-dimensional ring-shaped spin chains. We find that the total success probability can be significantly enhanced by phase shift control when the communication channel consists of two odd chains. The average time to gain unit success probability is discussed, showing that a proper phase shift can be used to enhance the efficiency of state transmission.展开更多
基金Supported by the Fundamental Research Funds for the Central Universitiesthe Funding for Outstanding Doctoral Dissertation in Nanjing University of Aeronautics and Astronautics(BCXJ11-01)~~
文摘An improved in-plane insensitive double-aperture digital speckle shearing interferometric technique is proposed to measure the first derivative of out-of-plane displacement （slope）. The temporal phase-shifting method is used for the quantitative analysis of fringes. The designed system employs a double-aperture arrange- ment placed in front of the imaging lens. A glass wedge covers one of the two apertures to introduce a laterally shear. The experimental specimen is a circular aluminum plate, clamped along its edge and subjected to both out- of-plane deflection and in-plane rotation. Experimental results show that the fringes obtained from the proposed optical configuration represent pure slope contour distributions, and that the contributions from the in-plane dis- placement components are completely eliminated. Theoretical and experimental results are in good agreement.
基金supported by grants from the Medical Scientific Research Foundation and Bureau of Health Care for Senior Officials of Zhejiang Province,China (No.2007B026)the National Natural Science Foundation of China (No.0471819)
文摘OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell linesand used SELDI-TOF-MS to detect the differential expressionof the proteins in the 2 cell lines.The proteomic spectra weregenerated using weak cation exchange chips.The biomarkerswere validated by analyzing serum proteins or peptides in ovariancancer patients,relapsed ovarian cancer patients,patients withbenign ovarian tumors,and healthy people.RESULTS Four proteins in the culture supernatant fromHO-8910PM cells were up-regulated,relative to the culturesupernatant of HO-8910 cells.One protein (3,144 Da m/z value)was up-regulated in both the cell lysis solution and in the culturesupernatant of HO-8910PM cells.In addition,expression of the3,144 Da m/z protein differed significantly between serum fromthe 26 ovarian cancer patients,from the 22 relapsed ovarianpatients and from the 37 healthy women (P<0.01).However,therewas no difference between patients with benign ovarian tumorsand healthy people (P>0.5).CONCLUSION Ovarian cancer cell lines with high or lowmetastatic potential have distinct protein profiles.Protein 3,144Da m/z could be a useful biomarker for diagnosing ovarian cancermetastasis.
文摘Spiking deconvolution is a standard Wiener Levinson algorithm. The autocorrelation of the design time gate is computed and there is a specified taper on the design gate before the autoeorrelation is done. The standard equations are set up, prewhitening is added to the zero lag value of the autocorrelation and the matrix is inverted to derive the spiking operator. In this study, the authors describe a technique for performing spiking deconvolution on prestack time migration （PSTM） data, to test the effect of operator length and percent prewhitening in spiking deconvolution and apply spiking deconvolution trace by trace, with operator lengths 15ms, 10 ms and 5 ms when percent prewhitening 0% , 40ms and 60ms for percent prewhitening 1%. The results show when prewhitening is 0% the shorter operator gives better results, but when value of prewhitening is bigger than 0% it is better to use longer operator lengths.
基金supported by the“Strategic Priority Research Program”of the Chinese Academy of Sciences(XDB11020700)CPSF-CAS Joint Foundation for Excellent Postdoctoral Fellows(2016LH00012)+1 种基金Strategic Priority Research Program of the Chinese Academy of Sciences(QYZDB-SSW-SMC021)the National Natural Science Foundation of China(31772400)
文摘Land plants in natural soil form intimate relationships with the diverse root bacterial microbiota. A growing body of evidence shows that these microbes are important for plant growth and health. Root microbiota composition has been widely studied in several model plants and crops; however, little is known about how root microbiota vary throughout the plant's life cycle under field conditions. We performed longitudinal dense sampling in field trials to track the time-series shift of the root microbiota from two representative rice cultivars in two separate locations in China. We found that the rice root microbiota varied dramatically during the vegetative stages and stabilized from the beginning of the reproductive stage, after which the root microbiota underwent relatively minor changes until rice ripening. Notably, both rice genotype and geographical location influenced the patterns of root microbiota shift that occurred during plant growth. The relative abundance of Deltaproteobacteria in roots significantly increased overtime throughout the entire life cycle of rice, while that of Betaproteobacteria, Firmicutes, and Gammaproteobacteria decreased. By a machine learning approach, we identified biomarker taxa and established a model to correlate root microbiota with rice resident time in the field(e.g., Nitrospira accumulated from 5 weeks/tillering in field-grown rice). Our work provides insights into the process of rice root microbiota establishment.
基金supported by the National Natural Science Foundation of China(No.11374105)
文摘We demonstrate a photon counting laser ranging experiment with a four-channel single-photon detector(SPD). The multi-channel SPD improve the counting rate more than 4×10~7 cps, which makes possible for the distance measurement performed even in daylight. However, the time-correlated single-photon counting(TCSPC) technique cannot distill the signal easily while the fast moving targets are submersed in the strong background. We propose a dynamic TCSPC method for fast moving targets measurement by varying coincidence window in real time. In the experiment, we prove that targets with velocity of 5 km/s can be detected according to the method, while the echo rate is 20% with the background counts of more than 1.2×10~7 cps.
基金Supported by National Natural Science Foundation of China under Grant Nos.11005099,60677044the Fundamental Research Funds for the Central Universities under Grant No.201013037
文摘We investigate the effect of phase shift on the perfect state transfer through two parallel one-dimensional ring-shaped spin chains. We find that the total success probability can be significantly enhanced by phase shift control when the communication channel consists of two odd chains. The average time to gain unit success probability is discussed, showing that a proper phase shift can be used to enhance the efficiency of state transmission.
