The function of protein in long-range biological electron transfer is a question of debate. We report some preliminary results in femtosecond spectroscopic study of photosynthetic bacterial light-harvesting antenna co...The function of protein in long-range biological electron transfer is a question of debate. We report some preliminary results in femtosecond spectroscopic study of photosynthetic bacterial light-harvesting antenna complex assembled onto TiO2 nanoparticle with an average size of 8 nm in diameter. Crystal structure shows that photosynthetic bacterial antenna complex LH2 has a ring-like structure composed by alpha- and beta-apoprotein helices. The alpha- and beta-transmembrance helices construct two concentric cylinders with pigments bacteriochlorophyll a (Bchl a) and carotenoid (Car) buried inside the protein. We attempt to insert TiO2 nanoparticle into the cavity of the inner cylindrical hollow of LH2 to investigate the nature of the electron transfer between the excited-state Bchl a and the TiO2 nanoparticle. A significant decrease in the ground state bleaching recovery time constant for Bchl a at 850 run (B850) in respect to that of the Bchl a in free LH2 has been observed. By using the relation of distance-dependent long-range electron transfer rate in protein, the distance between the donor B850 and the acceptor TiO2 nanoparticle has been estimated, which is about 0.6 nm. The proposed method of assembling proteins onto wide-gap semiconductor nanoparticle can be a promising way to determine the role of the protein playing in biological electron transfer processes.展开更多
The quality factor Q, which reflects the energy attenuation of seismic waves in subsurface media, is a diagnostic tool for hydrocarbon detection and reservoir characterization. In this paper, we propose a new Q extrac...The quality factor Q, which reflects the energy attenuation of seismic waves in subsurface media, is a diagnostic tool for hydrocarbon detection and reservoir characterization. In this paper, we propose a new Q extraction method based on the energy ratio before and after the wavelet attenuation, named the energy-ratio method（ERM）. The proposed method uses multipoint signal data in the time domain to estimate the wavelet energy without invoking the source wavelet spectrum, which is necessary in conventional Q extraction methods, and is applicable to any source wavelet spectrum; however, it requires high-precision seismic data. Forward zero-offset VSP modeling suggests that the ERM can be used for reliable Q inversion after nonintrinsic attenuation（geometric dispersion, refl ection, and transmission loss） compensation. The application to real zero-offset VSP data shows that the Q values extracted by the ERM and spectral ratio methods are identical, which proves the reliability of the new method.展开更多
Laboratory tests were conducted to study the effects of curing time, cement ratio and seawater pressure on cement soil deterioration formed at simulative marine soft clay sites. Deterioration depth was determined on t...Laboratory tests were conducted to study the effects of curing time, cement ratio and seawater pressure on cement soil deterioration formed at simulative marine soft clay sites. Deterioration depth was determined on the basis of characteristics of penetration resistance and penetration depth curves, and the deterioration depth of cement soil with the cement ratio of 7%, reached 31.8 mm after 720 d. Results of research indicated that deterioration extended quickly under seawater environment and the deterioration depth increased with the prolonging curing time. In addition, the water pressure could speed up deterioration. With the increase of cement content, the strength of cement soil increased obviously. At the same time, the deterioration depth decreased significantly. The concentration of calcium ion in the cement stabilized soil increased with the increase of depth, while that of magnesium ion gradually decreased. The variations were consistent with energy dispersive spectrometer(EDS)analysis results, and the calcium concentration with depth was in a good consistency with strength distribution at long term. The results showed that the deterioration became more serious with the curing time, and it was related to calcium leaching.展开更多
The mono-frequency peak luminosity and the corresponding photon energy of the time-integrated （L~, Eg） and peak time （L~, ffp） vfv spectra were derived for a sample of 38 redshift-known Fermi GRBs by fitting the s...The mono-frequency peak luminosity and the corresponding photon energy of the time-integrated （L~, Eg） and peak time （L~, ffp） vfv spectra were derived for a sample of 38 redshift-known Fermi GRBs by fitting the spectra with the Band function. It was found that Ept is generally consistent with Ep, and Lp is averagely three times larger than Lp. The slope of the Lp1 -Etp relation was consistent with that of the Lps - Eps relation. The photon indices in the peak time spectrum, particularly, the index of the low energy end was, were statistically larger than that in the time-integrated spectrum. These results indicate that Lp and Ep are dominated by Lp1 and Ept, respectively. The difference of the spectral indices between the time-integrated and peak time spectra may be because of the overlap effect of a series of time-resolved spectra within a GRB. Our simulations, which were based on the observed spectral evolution and correlation between the energy flux and the peak energy within individual GRBs support our speculations. The Lt - Et, relation may be less contaminated by the overla!a effect, and it would may be an intrinsic feature of radiation ohvsics.展开更多
文摘The function of protein in long-range biological electron transfer is a question of debate. We report some preliminary results in femtosecond spectroscopic study of photosynthetic bacterial light-harvesting antenna complex assembled onto TiO2 nanoparticle with an average size of 8 nm in diameter. Crystal structure shows that photosynthetic bacterial antenna complex LH2 has a ring-like structure composed by alpha- and beta-apoprotein helices. The alpha- and beta-transmembrance helices construct two concentric cylinders with pigments bacteriochlorophyll a (Bchl a) and carotenoid (Car) buried inside the protein. We attempt to insert TiO2 nanoparticle into the cavity of the inner cylindrical hollow of LH2 to investigate the nature of the electron transfer between the excited-state Bchl a and the TiO2 nanoparticle. A significant decrease in the ground state bleaching recovery time constant for Bchl a at 850 run (B850) in respect to that of the Bchl a in free LH2 has been observed. By using the relation of distance-dependent long-range electron transfer rate in protein, the distance between the donor B850 and the acceptor TiO2 nanoparticle has been estimated, which is about 0.6 nm. The proposed method of assembling proteins onto wide-gap semiconductor nanoparticle can be a promising way to determine the role of the protein playing in biological electron transfer processes.
基金supported by the Major Project of the Ministry of Science and Technology of China(No.2011ZX05024-001-01)National Nature Science Foundation of China(No.41140033)
文摘The quality factor Q, which reflects the energy attenuation of seismic waves in subsurface media, is a diagnostic tool for hydrocarbon detection and reservoir characterization. In this paper, we propose a new Q extraction method based on the energy ratio before and after the wavelet attenuation, named the energy-ratio method（ERM）. The proposed method uses multipoint signal data in the time domain to estimate the wavelet energy without invoking the source wavelet spectrum, which is necessary in conventional Q extraction methods, and is applicable to any source wavelet spectrum; however, it requires high-precision seismic data. Forward zero-offset VSP modeling suggests that the ERM can be used for reliable Q inversion after nonintrinsic attenuation（geometric dispersion, refl ection, and transmission loss） compensation. The application to real zero-offset VSP data shows that the Q values extracted by the ERM and spectral ratio methods are identical, which proves the reliability of the new method.
基金Supported by the National Natural Science Foundation of China(No.50779062)
文摘Laboratory tests were conducted to study the effects of curing time, cement ratio and seawater pressure on cement soil deterioration formed at simulative marine soft clay sites. Deterioration depth was determined on the basis of characteristics of penetration resistance and penetration depth curves, and the deterioration depth of cement soil with the cement ratio of 7%, reached 31.8 mm after 720 d. Results of research indicated that deterioration extended quickly under seawater environment and the deterioration depth increased with the prolonging curing time. In addition, the water pressure could speed up deterioration. With the increase of cement content, the strength of cement soil increased obviously. At the same time, the deterioration depth decreased significantly. The concentration of calcium ion in the cement stabilized soil increased with the increase of depth, while that of magnesium ion gradually decreased. The variations were consistent with energy dispersive spectrometer(EDS)analysis results, and the calcium concentration with depth was in a good consistency with strength distribution at long term. The results showed that the deterioration became more serious with the curing time, and it was related to calcium leaching.
基金supported by the National Basic Research Program of China(Grant No.2014CB845800)the National Natural Science Foundation of China(Grant No.11025313)+1 种基金Guangxi Science Foundation(Grant No.2013GXNSFFA019001)Key Laboratory for the Structure and Evolution of Celestial Objects of Chinese Academy of Sciences,and the Strategic Priority Research Program"The Emergence of Cosmological Structures"of the Chinese Academy of Sciences(Grant No.XDB09000000)
文摘The mono-frequency peak luminosity and the corresponding photon energy of the time-integrated （L~, Eg） and peak time （L~, ffp） vfv spectra were derived for a sample of 38 redshift-known Fermi GRBs by fitting the spectra with the Band function. It was found that Ept is generally consistent with Ep, and Lp is averagely three times larger than Lp. The slope of the Lp1 -Etp relation was consistent with that of the Lps - Eps relation. The photon indices in the peak time spectrum, particularly, the index of the low energy end was, were statistically larger than that in the time-integrated spectrum. These results indicate that Lp and Ep are dominated by Lp1 and Ept, respectively. The difference of the spectral indices between the time-integrated and peak time spectra may be because of the overlap effect of a series of time-resolved spectra within a GRB. Our simulations, which were based on the observed spectral evolution and correlation between the energy flux and the peak energy within individual GRBs support our speculations. The Lt - Et, relation may be less contaminated by the overla!a effect, and it would may be an intrinsic feature of radiation ohvsics.
