Cloud computing and storage services allow clients to move their data center and applications to centralized large data centers and thus avoid the burden of local data storage and maintenance.However,this poses new ch...Cloud computing and storage services allow clients to move their data center and applications to centralized large data centers and thus avoid the burden of local data storage and maintenance.However,this poses new challenges related to creating secure and reliable data storage over unreliable service providers.In this study,we address the problem of ensuring the integrity of data storage in cloud computing.In particular,we consider methods for reducing the burden of generating a constant amount of metadata at the client side.By exploiting some good attributes of the bilinear group,we can devise a simple and efficient audit service for public verification of untrusted and outsourced storage,which can be important for achieving widespread deployment of cloud computing.Whereas many prior studies on ensuring remote data integrity did not consider the burden of generating verification metadata at the client side,the objective of this study is to resolve this issue.Moreover,our scheme also supports data dynamics and public verifiability.Extensive security and performance analysis shows that the proposed scheme is highly efficient and provably secure.展开更多
Background Approximately 10%of patients with gastric cancer(GC)have a genetic predisposition toward the disease.However,there is scant knowledge regarding germline mutations in predisposing genes in the Chinese GC pop...Background Approximately 10%of patients with gastric cancer(GC)have a genetic predisposition toward the disease.However,there is scant knowledge regarding germline mutations in predisposing genes in the Chinese GC population.This study aimed to determine the spectrum and distribution of predisposing gene mutations among Chinese GC patients known to have hereditary high-risk factors for cancer.Methods A total of 40 GC patients from 40 families were recruited from seven medical institutions in China.Next-generation sequencing was performed on 171 genes associated with cancer predisposition.For probands carrying pathogenic/likely pathogenic germline variants,Sanger sequencing was applied to validate the variants in the probands as well as their relatives.Results According to sequencing results,25.0%(10/40)of the patients carried a combined total of 10 pathogenic or likely pathogenic germline variants involving nine different genes:CDH1(n=1),MLH1(n=1),MSH2(n=1),CHEK2(n=1),BLM(n=1),EXT2(n=1),PALB2(n=1),ERCC2(n=1),and SPINK1(n=2).In addition,129 variants of uncertain significance were identified in 27 patients.Conclusions This study indicates that approximately one in every four Chinese GC patients with hereditary high risk factors may harbor pathogenic/likely pathogenic germline alterations in cancer-susceptibility genes.The results further indicate a unique genetic background for GC among Chinese patients.展开更多
基金the National Natural Science Foundation of China，the National Basic Research Program of China ("973" Program) the National High Technology Research and Development Program of China ("863" Program)
文摘Cloud computing and storage services allow clients to move their data center and applications to centralized large data centers and thus avoid the burden of local data storage and maintenance.However,this poses new challenges related to creating secure and reliable data storage over unreliable service providers.In this study,we address the problem of ensuring the integrity of data storage in cloud computing.In particular,we consider methods for reducing the burden of generating a constant amount of metadata at the client side.By exploiting some good attributes of the bilinear group,we can devise a simple and efficient audit service for public verification of untrusted and outsourced storage,which can be important for achieving widespread deployment of cloud computing.Whereas many prior studies on ensuring remote data integrity did not consider the burden of generating verification metadata at the client side,the objective of this study is to resolve this issue.Moreover,our scheme also supports data dynamics and public verifiability.Extensive security and performance analysis shows that the proposed scheme is highly efficient and provably secure.
文摘Background Approximately 10%of patients with gastric cancer(GC)have a genetic predisposition toward the disease.However,there is scant knowledge regarding germline mutations in predisposing genes in the Chinese GC population.This study aimed to determine the spectrum and distribution of predisposing gene mutations among Chinese GC patients known to have hereditary high-risk factors for cancer.Methods A total of 40 GC patients from 40 families were recruited from seven medical institutions in China.Next-generation sequencing was performed on 171 genes associated with cancer predisposition.For probands carrying pathogenic/likely pathogenic germline variants,Sanger sequencing was applied to validate the variants in the probands as well as their relatives.Results According to sequencing results,25.0%(10/40)of the patients carried a combined total of 10 pathogenic or likely pathogenic germline variants involving nine different genes:CDH1(n=1),MLH1(n=1),MSH2(n=1),CHEK2(n=1),BLM(n=1),EXT2(n=1),PALB2(n=1),ERCC2(n=1),and SPINK1(n=2).In addition,129 variants of uncertain significance were identified in 27 patients.Conclusions This study indicates that approximately one in every four Chinese GC patients with hereditary high risk factors may harbor pathogenic/likely pathogenic germline alterations in cancer-susceptibility genes.The results further indicate a unique genetic background for GC among Chinese patients.
