【目的】在海南省文昌市椰子大观园露地种植的春羽(Philodendron bipinnatifidum Schott ex Endl.)上发现一种叶斑病,明确了该病害的病原物和部分生物学特性。【方法】通过分离培养和接种试验,经传统形态学及分子生物学方法鉴定了致病菌...【目的】在海南省文昌市椰子大观园露地种植的春羽(Philodendron bipinnatifidum Schott ex Endl.)上发现一种叶斑病,明确了该病害的病原物和部分生物学特性。【方法】通过分离培养和接种试验,经传统形态学及分子生物学方法鉴定了致病菌,采用十字交叉法和血球计数板法测定该病原菌的生物学特性。【结果】该病原菌鉴定为胶孢炭疽菌(Colletotrichum gloeosporioides),菌丝初期为白色,后转为青灰色,产生红色孢子堆。分生孢子椭圆形,大小为6.14~14.69μm×2.96~6.49μm。菌丝生长最适温度为25℃,最适pH值为8~9;产孢最适温度为30℃,最适pH为9。此菌能利用多种碳源,但利用能力有差异,最适菌丝生长的碳源为蔗糖;最适氮源为硝酸钠,硝酸铵和硫酸铵作为氮源培养基明显不利于菌丝生长。【结论】首次对春羽炭疽病病原进行分离鉴定,该病原菌最适生长温度为25℃,pH为9,最适碳氮源分别为蔗糖和硝酸钠。展开更多
A new triterpenoid saponin, namely pomatoside A (3) 13beta, 28-epoxy-16-oleananone-3-O-[alpha-L-rhamnopyranosyl-(1 -->6)-O-beta-D-glucopyranosyl-(1 -->4)-O-beta-D-glucopyranosyl-(1 -->6)-O-beta-D-glucopyranos...A new triterpenoid saponin, namely pomatoside A (3) 13beta, 28-epoxy-16-oleananone-3-O-[alpha-L-rhamnopyranosyl-(1 -->6)-O-beta-D-glucopyranosyl-(1 -->4)-O-beta-D-glucopyranosyl-(1 -->6)-O-beta-D-glucopyranosyl-(1 -->2)]-beta-D-glucopyranoside, together with twelve known compounds, was isolated for the first time from Pomatosace filicula Maxim., a monotypic endemic plant, grown in the Qinghai-Xizang Plateau of China. Their structures were elucidated by spectroscopic analyses and chemical methods, especially 2D-NMR.展开更多
文摘A new triterpenoid saponin, namely pomatoside A (3) 13beta, 28-epoxy-16-oleananone-3-O-[alpha-L-rhamnopyranosyl-(1 -->6)-O-beta-D-glucopyranosyl-(1 -->4)-O-beta-D-glucopyranosyl-(1 -->6)-O-beta-D-glucopyranosyl-(1 -->2)]-beta-D-glucopyranoside, together with twelve known compounds, was isolated for the first time from Pomatosace filicula Maxim., a monotypic endemic plant, grown in the Qinghai-Xizang Plateau of China. Their structures were elucidated by spectroscopic analyses and chemical methods, especially 2D-NMR.