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水稻质体发育过程中DNA分子动态变化的初步研究
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作者 莫永胜 张玉麟 《自然杂志》 1990年第10期699-700,共2页
有关高等植物质体发育过程中DNA分子的动态变化,迄今仅在小麦中有报道。本文报道我们应用DNA专一荧光染料DAPI(4′,6-diamidino-2-phenylindole)同质体DNA的结合。
关键词 DNA 荧光染料 DAPI 显微光度法 亲缘性 微观察 暗培养 致密结构 三维结构 试验方
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Efficacy of Photogem(Hematoporphyrin Derivative) as a Photoactivatable Larvicide against Aedes aegypti (Diptera: Culicidae) Larvae
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作者 Larissa Marila de Souza Natalia Mayumi Inada +3 位作者 Sebastiao Pratavieira Juliano Jose Corbi Cristina Kurachi Vanderlei Salvador Bagnato 《Journal of Life Sciences》 2017年第2期74-81,共8页
Dengue vector is responsible for millions of deaths every year and has caused disastrous impacts on health systems. The continuous use of chemical insecticides, such as carbamates, pyrethroids and organophosphates gen... Dengue vector is responsible for millions of deaths every year and has caused disastrous impacts on health systems. The continuous use of chemical insecticides, such as carbamates, pyrethroids and organophosphates generates resistant populations of the mosquito, therefore, new control methods must be investigated. The joint action of the population and guidelines for preventing the reproduction of the mosquito associated with the use of photoactivatable insecticides can be the alternative for the control of epidemiological outbreaks in affected regions. In this study, the photo-larvicidal activity of Photogem^(PG), a derivative of hematoporphyrin, was investigated against 2nd-early 3rd instar of Aedes aegypti larvae (Diptera: Culicidae) under different lighting conditions (artificial lighting system and sunlight). The dynamics of PG accumulation was characterized by CLSM (confocal laser scanning microscopy) and total time PG eliminationin solution was investigated by ultraviolet-visible spectrophotometry. The maximum photo-activity of PG was observed in 0.5 h under sunlight exposure which achieved 100% larval mortality. Fluorescence images showed a uniform distribution of PG along the digestive tract. PG remained stable in the sunlight for 48 h and in an artificial lighting system for longer periods, therefore, it can be used for the control ofAedes aegypti larvae as a new alternative to chemical insecticides. The method is considered environmentally friendly due to its rapid degradation in the presence of light. Further studies are required, so that the potential of the technique can be explored in real breeding places. 展开更多
关键词 Photoactivatable larvicide PG hematoporphyrin derivative Aedes aegypti natural lighting artificial lighting.
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UV-Vis Spectrophotometry and AFM Observation of Diethyl Dithiophosphate Adsorption on Chalcopyrite at Varied pHs 被引量:1
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作者 Himawan T. B. M. Petrus Tsuyoshi Hirajima +1 位作者 Keiko Sasaki Hideyuki Okamoto 《Journal of Chemistry and Chemical Engineering》 2011年第11期1006-1013,共8页
Adsorption of diehtyl dithiophosphate on chalcopyrite has been studied using UV-visible spectroscopy at pH values of 4, 6, and 9. It was found that the adsorption of diethyl dithiophosphate decreased with the increasi... Adsorption of diehtyl dithiophosphate on chalcopyrite has been studied using UV-visible spectroscopy at pH values of 4, 6, and 9. It was found that the adsorption of diethyl dithiophosphate decreased with the increasing pH treatments. The inhibition of diethyl dithiophosphate adsorption was found prominent in higher pH as a result of metal hydroxide species formation onto chalcopyrite surface. First order kinetic has been proposed and represented the adsorption mechanism. Flotation test using Hallimond tube has also been conducted and the results were consistent with the proposed mechanism. Furthermore, the morphological changes of the treated chalcopyrite were observed using Atomic Force Microscopy (AFM) showing the propensity of growth of islands, the new surfaces as products of the reaction. The hydrophobicity was measured in the form of force of adhesion. The results resembled with the approached mechanism. 展开更多
关键词 CHALCOPYRITE diethyl dithiophosphate ADSORPTION kinetic study AFM FLOATABILITY
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3D Surface Morphology Measurement and Auto-focusing System
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作者 CHEN Qi ZANG Huai-pei 《Semiconductor Photonics and Technology》 CAS 2005年第3期208-211,共4页
When interference microscope measures the surface rough of the micromechanical device, as soon as the work distance of interference microscope and the depth of field is shortened, the interference images become slur f... When interference microscope measures the surface rough of the micromechanical device, as soon as the work distance of interference microscope and the depth of field is shortened, the interference images become slur for the measured object if there has small interference after clear focus. The auto-focusing system is introduced into the interference microscope, the system can obtain high definition interference image rapidly,and can improve the measuring velocity and measuring precision. The system is characterized by auto-focusing range of ±150 μm, auto-focusing precision of ±0.3 μm, auto-focusing time of 4~8 s. 展开更多
关键词 3D surface morphology Interference microscope AUTO-FOCUSING
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细胞移动抑制试验比较两株卡介菌种
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作者 刘纯谦 于淑萍 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 1990年第3期166-168,共3页
关键词 卡介苗 细胞移动抑制 显微光度法
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